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1.
Biochemistry ; 36(23): 7050-7, 1997 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-9188703

RESUMEN

Many bacterial hemoproteins involved in heme acquisition have been isolated recently, comprising outer membrane receptors and extracellular heme-binding protein. The mechanisms by which these proteins extract heme have not been described up to now. One such protein, HasA, which can bind free heme as well as capture it from hemoglobin, is secreted by the Gram-negative bacteria Serratia marcescens under iron deficiency conditions. The fact that HasA does not present sequence similarities with other known hemoproteins suggests that it possesses a new type of heme binding site. This work describes the main physicochemical properties of HasA, essential for understanding its function. HasA is a monomer of 19 kDa that binds one b heme per molecule with high affinity. The electron paramagnetic resonance spectra indicate that the heme iron is in a low-spin ferric state and that the two iron axial ligands are His and His-. The low oxidation-reduction potential value (-550 mV vs standard hydrogen electrode) of the heme bound to HasA suggests that heme could be exposed to the solvent. According to circular dichroism data, the binding of heme does not seem to modify the conformation of HasA.


Asunto(s)
Proteínas Bacterianas/aislamiento & purificación , Proteínas Portadoras , Hemo/metabolismo , Hierro/metabolismo , Proteínas de la Membrana/aislamiento & purificación , Secuencia de Aminoácidos , Animales , Proteínas Bacterianas/química , Bovinos , Cromatografía en Gel , Dicroismo Circular , Espectroscopía de Resonancia por Spin del Electrón , Cinética , Proteínas de la Membrana/química , Datos de Secuencia Molecular , Unión Proteica , Espectrometría de Fluorescencia , Espectrofotometría Ultravioleta
2.
Talanta ; 43(7): 1125-30, 1996 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-18966589

RESUMEN

A permselective-membrane pyrolytic graphite electrode was used to study the electrochemistry of [2Fe-2S] ferredoxin entrapped between the membrane and the graphite surface, in the presence of poly(l-Lysine). Factors influencing the electrode response [such as the concentration of poly(l-lysine), ionic strength and pH] were explored. The analytical performance of this permselective-membrane electrode, which allows 20 pmol of ferredoxin to be detected, was examined.

3.
Biochim Biophys Acta ; 1273(1): 51-61, 1996 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-8573595

RESUMEN

Three c-type cytochromes were isolated and characterized from the sulfate reducer Desulfovibrio africanus. A basic tetraheme cytochrome c3 of molecular mass 16 kDa was previously described and we have extended its characterization. Two other c3-type cytochromes, not previously observed, have also been characterized. These include an acidic tetraheme cytochrome c3 of molecular mass 15 kDa and an octaheme dimeric cytochrome c3 with a native size of 35 kDa. This is the first report of the presence of two distinct tetraheme cytochromes c3 in a Desulfovibrio species. The physico-chemical properties of the three cytochromes, including optical properties, iron content, cysteine and histidine content, N-terminal amino sequence and redox properties, are characteristic of cytochrome c3 family. The acidic tetraheme cytochrome c3 exhibited similar midpoint potential values for all four hemes (Em1 = -210 mV; Em2 = -240 mV; Em3 = -260 mV; Em4 = -270 mV), whereas in the basic tetraheme cytochrome c3 one heme had a much more positive potential than the others (Em1 = -90 mV; Em2 = -260 mV; Em3 = -280 mV; Em4 = -290 mV). The acidic tetraheme cytochrome c3 exhibited unique properties including amino-acid composition and poor reactivity towards hydrogenase. However, it is readily reduced by this enzyme in the presence of the basic cytochrome c3. The weak reactivity of the acidic tetraheme cytochrome c3 towards hydrogenase has been correlated with its low content of basic residues.


Asunto(s)
Grupo Citocromo c/química , Desulfovibrio/enzimología , Secuencia de Aminoácidos , Aminoácidos/análisis , Grupo Citocromo c/aislamiento & purificación , Electroquímica , Hemo/análisis , Datos de Secuencia Molecular , Peso Molecular , Oxidación-Reducción , Espectrofotometría , Especificidad por Sustrato
4.
FEBS Lett ; 373(3): 280-4, 1995 Oct 16.
Artículo en Inglés | MEDLINE | ID: mdl-7589483

RESUMEN

The cytochrome c3 superfamily includes Desulfovibrio polyheme cytochromes c. We report the characteristic thermal stability parameters of the Desulfovibrio desulfuricans Norway (D.d.N.) cytochromes c3 (M(r) 13,000 and M(r) 26,000) and the Desulfovibrio vulgaris Hildenborough (D.v.H.) cytochrome c3 (M(r) 13,000) and high molecular mass cytochrome c (Hmc), as obtained with the help of electronic spectroscopy, voltammetric techniques and differential scanning calorimetry. The polyheme cytochromes are denatured over a wide range of temperatures: the D.v.H. cytochrome c3 is highly thermostable (Td = 121 degrees C) contrary to the D.d.N. protein (Td = 73 degrees C). The thermostability of the polyheme cytochromes is redox state dependent. The results are discussed in the light of the structural and functional relationships within the cytochrome c3 superfamily.


Asunto(s)
Grupo Citocromo c/metabolismo , Desulfovibrio/química , Rastreo Diferencial de Calorimetría , Grupo Citocromo c/química , Hemo/metabolismo , Oxidación-Reducción , Desnaturalización Proteica , Análisis Espectral , Temperatura , Termodinámica
5.
J Biol Chem ; 269(9): 6340-6, 1994 Mar 04.
Artículo en Inglés | MEDLINE | ID: mdl-8119983

RESUMEN

Cytochrome c3 (M(r) 13,000) is a tetrahemic cytochrome in which the four heme iron atoms are coordinated by 2 histidine residues at the axial positions. The presence of several oxidoreduction centers in the same molecule raises the question of their coupling. To investigate this mechanism, four single mutations were introduced in cytochrome c3 by site-directed mutagenesis, leading to the replacement of each histidine, the sixth axial ligand of the heme iron atom, by a methionine residue. Characterization of the new set of molecules using biochemical and biophysical techniques was carried out. The novel methionine was correctly coordinated to the iron atom of hemes 3 and 4 in H25M and H70M cytochromes c3, respectively, and this coordination induced a large increase in the oxidoreduction potential of the mutated heme. In contrast, in the case of H22M and H35M cytochromes c3, in which the corresponding methionine is in an oxidized form, only slight changes in redox potential values were observed. In H22M, H25M, and H35M cytochromes c3, two conformations of the molecule were possible, in which the methionine is either free or coordinated to the iron atom. The rate constants for the electron exchange reactions between the cytochrome mutants and the hydrogenase were measured using electrochemical techniques. Distinct behaviors were revealed depending on the mutation. The values of the rate constants for the electron exchange reactions are interpreted in terms of intramolecular electron exchange among the four hemes of the cytochrome.


Asunto(s)
Grupo Citocromo c/metabolismo , Desulfovibrio/metabolismo , Hemo/metabolismo , Secuencia de Bases , Sitios de Unión , Clonación Molecular , Grupo Citocromo c/biosíntesis , Grupo Citocromo c/química , Desulfovibrio/genética , Desulfovibrio vulgaris/metabolismo , Escherichia coli , Ligandos , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Oligodesoxirribonucleótidos , Oxidación-Reducción , Plásmidos , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Relación Estructura-Actividad
6.
Biochimie ; 76(7): 605-13, 1994.
Artículo en Inglés | MEDLINE | ID: mdl-7893812

RESUMEN

C-type cytochromes are classified into two main groups: i) cytochromes which give fast electrochemical responses at the conventional electrodes in the absence of any promoter (eg multi-heme cytochromes c3); ii) cytochromes which need the presence of promoters or the use of modified electrodes to exhibit fast electrochemical responses (eg one-heme mitochondrial cytochrome c). In the latter case, careful design of electrode surface and composition of the solution are required for the attainment of rapid and reversible electron-exchange reactions. Some general considerations are given on the 'electrochemical model'. In particular, binding interactions between the electrode and the protein can take place in a similar manner to that occurring between physiological partner proteins. Electrochemistry when coupled to other physical techniques can give more complete insights in the relationship between the redox properties, structure and function of c-type cytochromes. In particular, in the case of polyheme cytochromes, promising results are expected from the study of site-directed mutagenesis-modified cytochrome c3.


Asunto(s)
Grupo Citocromo c/química , Grupo Citocromo c/metabolismo , Animales , Electroquímica , Transporte de Electrón , Hemo/metabolismo , Caballos , Cinética , Mitocondrias Cardíacas/metabolismo , Oxidación-Reducción , Pseudomonas aeruginosa/metabolismo , Bacterias Reductoras del Azufre/metabolismo
7.
Biochem Biophys Res Commun ; 189(2): 633-9, 1992 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-1335243

RESUMEN

In order to understand the electron transfer mechanisms for the [Fe] and [Ni-Fe] hydrogenases, a kinetic study of cytochrome c3 reduction has been undertaken. Cyclic voltammetry and controlled-potential amperometry techniques have been used to investigate the intermolecular electron-transfer reaction between cytochrome c3 and [Fe] hydrogenase from Desulfovibrio vulgaris Hildenborough. Electron-transfer cross-reactions between [Fe] or [Ni-Fe-Se] hydrogenase and cytochrome c3 from Desulfovibrio vulgaris Hildenborough or Desulfovibrio desulfuricans Norway have been studied. Some structural implications are considered from these experimental data.


Asunto(s)
Grupo Citocromo c/metabolismo , Desulfovibrio/metabolismo , Hidrogenasas/metabolismo , Hierro/metabolismo , Níquel/metabolismo , Selenio/metabolismo , Desulfovibrio vulgaris/metabolismo , Electroquímica/métodos , Transporte de Electrón , Cinética , Oxidación-Reducción
8.
J Biol Chem ; 267(24): 16851-8, 1992 Aug 25.
Artículo en Inglés | MEDLINE | ID: mdl-1324913

RESUMEN

The nature of the axial ligands of a heme group is an important factor in maintaining the oxidation-reduction potential of a c-type cytochrome. Cytochrome c3 from Desulfovibrio vulgaris Hildenborough contains four bis-histidinyl coordinated hemes with low oxidation-reduction potentials. Site-directed mutagenesis was used to generate a mutant in which histidine 70, the sixth axial ligand of heme 4, has been replaced by a methionine. The mutant protein was expressed in Desulfovibrio desulfuricans G200 at a level similar to the wild type cytochrome. A model for the three-dimensional structure of D. vulgaris Hildenborough cytochrome c3 was generated on the basis of the crystal structure of D. vulgaris Miyazaki cytochrome c3 in order to investigate the effects of the H70M mutation. The model, together with NMR data, suggested that methionine 70 has effectively replaced histidine 70 as the sixth axial ligand of heme 4 without significant alteration of the structure. A large increase of at least 200 mV of one of the four oxidation-reduction potentials was observed by electrochemistry and is interpreted in terms of structure/potential relationships.


Asunto(s)
Grupo Citocromo c/química , Hemo/metabolismo , Mutagénesis Sitio-Dirigida , Secuencia de Aminoácidos , Secuencia de Bases , Sitios de Unión , Grupo Citocromo c/genética , Grupo Citocromo c/metabolismo , Desulfovibrio vulgaris/genética , Desulfovibrio vulgaris/metabolismo , Vectores Genéticos , Ligandos , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Datos de Secuencia Molecular , Oligodesoxirribonucleótidos , Conformación Proteica , Mapeo Restrictivo
9.
Biochemistry ; 31(12): 3281-8, 1992 Mar 31.
Artículo en Inglés | MEDLINE | ID: mdl-1313289

RESUMEN

The gene of high molecular weight, multiheme cytochrome c (Hmc) from the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough has been overexpressed in Desulfovibrio desulfuricans G200. The recombinant protein has been purified. Its molecular weight (65,600), amino acid composition, and NH2-terminal sequence were found to be identical to those of the wild-type protein. The recombinant protein has been spectroscopically characterized (optical spectrum, EPR, circular dichroism) and compared to the wild-type protein. We have found 16 hemes per molecule by iron analysis and the pyridine hemochrome test. Both high- and low-spin features were observed in the EPR spectrum. A detailed spin quantitation analysis indicates 1 or 2 high-spin hemes and 14 or 15 low-spin hemes per molecule. The redox potentials of the hemes determined by voltammetric techniques gave an average of three different values, 0, -100, and -250 mV (versus NHE), for the wild-type and the recombinant cytochrome. The low potential values are similar to the values observed for the bis(histidinyl) coordinated hemes of cytochrome c3. A comparison of the arrangement of heme binding sites and coordinated histidines in the amino acid sequences of cytochrome c3 and Hmc has shown that the latter contains four domains, three of which are complete c3-like domains, while the fourth represents an incomplete c3-like domain which may contain His-Met coordinated hemes. These data are in agreement with the detailed study of the number and types of hemes reported in this paper.


Asunto(s)
Grupo Citocromo c/genética , Desulfovibrio vulgaris/genética , Vectores Genéticos , Secuencia de Aminoácidos , Aminoácidos/análisis , Fenómenos Químicos , Química Física , Dicroismo Circular , Grupo Citocromo c/biosíntesis , Grupo Citocromo c/química , Desulfovibrio vulgaris/enzimología , Electroquímica , Espectroscopía de Resonancia por Spin del Electrón , Genes Bacterianos , Hemo/análisis , Hierro/análisis , Datos de Secuencia Molecular , Peso Molecular , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación
10.
Biochemistry ; 30(48): 11459-65, 1991 Dec 03.
Artículo en Inglés | MEDLINE | ID: mdl-1660303

RESUMEN

The electrochemical behavior of tetraheme cytochrome c554 from Nitrosomonas europaea has been studied by thin-layer spectroelectrochemistry, cyclic voltammetry, differential pulse voltammetry, and alternating current voltammetry. Three redox couples were detected. Midpoint potentials for the high-m intermediate-, and low-potential couples are +47, -147, and -276 mV, respectively, from the spectroelectrochemical measurements and +50, -120, and -225 mV, respectively, from the voltammetry measurements. A coulometric titration shows that two electrons are taken up by the high-potential couple and one each is taken up by the intermediate- and low-potential couples. Results from the spectroelectrochemical titration at carefully chosen wavelengths indicate that the intermediate- and low-potential couples obey simple Nernstian behavior. The electrochemical behavior of the high-potential couple is apparently not truly Nernstian but is most consistent with two sites exhibiting slight positive cooperativity. Spectral changes associated with the three couples reveal distinctive features in the reduced-minus-oxidized difference spectra. The difference spectrum of the high-potential pair of hemes suggest a mixture of a high-spin heme and a low-spin heme with maxima at 424 and 432 nm. The difference spectrum of the intermediate-potential heme is low spin with a split Soret with maxima at 414 and 424 nm. The difference spectrum of the low-potential heme also shows a split Soret with maxima at 418 and 432 nm.


Asunto(s)
Grupo Citocromo c/química , Hemo/química , Nitrosomonas/química , Electroquímica , Oxidación-Reducción , Potenciometría , Espectrofotometría , Compuestos de Sulfhidrilo/farmacología
11.
Biochem Biophys Res Commun ; 179(1): 605-10, 1991 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-1652960

RESUMEN

The octaheme cytochrome c3 (Mr 26000) from Desulfovibrio desulfuricans Norway was studied using cyclic voltammetry at the pyrolytic graphite electrode. The kinetics of reduction of the octaheme cytochrome c3 (Mr 26000) from D. desulfuricans Norway by the Ni-Fe-Se hydrogenase purified from the same organism was investigated by an electrochemical method. From cyclic voltammetry experiments a value of 8.108M-1S-1 was obtained for the second order homogenous rate constant of the electron transfer between the two proteins. Results are compared with similar experiments performed on the electron exchange between the tetrahemic cytochrome c3 (Mr 13000) and hydrogenase.


Asunto(s)
Grupo Citocromo c/metabolismo , Desulfovibrio/metabolismo , Hidrogenasas/metabolismo , Grupo Citocromo c/aislamiento & purificación , Transporte de Electrón , Hemo/análisis , Hemo/metabolismo , Hidrogenasas/aislamiento & purificación , Cinética , Peso Molecular , Concentración Osmolar
12.
Biochem Biophys Res Commun ; 159(2): 670-6, 1989 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-2539120

RESUMEN

Cytochrome c3 (Mr 26,000) has been characterized in Desulfovibrio vulgaris (Hildenborough) and its properties compared with polyhemic cytochromes c isolated from the same organism and from D. desulfuricans (Norway). It can be described as an octaheme cytochrome c3 constituted of two identical subunits. Absorption spectrum is similar to cytochrome c3 (Mr 13,000) and individual redox potentials have an average value of -180 mV.3 The N terminal sequence is compared with an homologous cytochrome isolated from D. desulfuricans Norway.


Asunto(s)
Grupo Citocromo c/aislamiento & purificación , Desulfovibrio/análisis , Secuencia de Aminoácidos , Aminoácidos/aislamiento & purificación , Punto Isoeléctrico , Sustancias Macromoleculares , Datos de Secuencia Molecular , Peso Molecular , Oxidación-Reducción , Especificidad de la Especie , Ultracentrifugación
13.
Biochem Biophys Res Commun ; 147(2): 818-23, 1987 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-2820417

RESUMEN

In view of the assignment of the four redox potentials values to the four heme groups in the crystallographic structure of Desulfovibrio desulfuricans Norway cytochrome c3, a biochemical approach is reported. A singly modified cytochrome c3 on arginine 73 has been prepared. The study of the redox properties of the modified cytochrome by electrochemistry together with the graphic modelisation of the molecule allow to assign the highest redox potential (-165 mV) to the heme 4 in the three dimensional structure.


Asunto(s)
Arginina , Grupo Citocromo c , Desulfovibrio/análisis , Hemo , Fenómenos Químicos , Química , Gráficos por Computador , Electroquímica , Espectroscopía de Resonancia Magnética , Oxidación-Reducción
14.
Biochem Biophys Res Commun ; 147(3): 1289-94, 1987 Sep 30.
Artículo en Inglés | MEDLINE | ID: mdl-2822044

RESUMEN

The kinetics of the reduction of the Desulfovibrio desulfuricans Norway cytochrome c3 by its physiological partner hydrogenase, in the presence of hydrogen, was investigated by an electrochemical method; from cyclic voltammetry experiments a value of 3 X 10(7) M-1 s-1 was obtained for the second-order rate constant. Results are discussed in terms of specific interactions between physiological partner proteins.


Asunto(s)
Grupo Citocromo c/fisiología , Desulfovibrio/fisiología , Hidrogenasas/metabolismo , Electroquímica , Transporte de Electrón , Cinética , Oxidación-Reducción
15.
J Bacteriol ; 159(3): 1040-6, 1984 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-6090384

RESUMEN

A c3 type cytochrome has been purified from the thermophilic, non-spore-forming, sulfate-reducing bacterium Thermodesulfobacterium commune. The purified protein was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gel filtration, and isoelectric focusing. A pI of 6.83 was observed. The molecular weight of the cytochrome was estimated to be ca. 13,000 from both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The hemoprotein exhibited absorption maxima at 530, 408.5, and 351 nm in the oxidized form and 551.5 (alpha band), 522.5 (beta band), and 418.5 nm (gamma band) in the reduced form. The extinction coefficients of T. commune cytochrome c3 were 130,000, 74,120, and 975,000 M-1 cm-1 at 551.5, 522.5, and 418.5 nm, respectively. It contains four hemes per molecule, on the basis of both the iron estimation and the extinction coefficient value of its pyridine hemochrome. The amino acid composition showed the presence of eight cysteine residues involved in heme binding. T. commune cytochrome c3 had low threonine, serine, and glycine contents and high glutamic acid and hydrophobic residue contents. The electrochemical study of T. commune cytochrome c3 by cyclic voltammetry and differential pulse polarography has shown that the cytochrome system behaves like a reversible system. Four redox potential values at Eh1 = -0.140 +/- 0.010 V, Eh2 = Eh3 = Eh4 = -0.280 +/- 0.010 V have been determined. T. commune cytochrome c3, which acts as the physiological electron carrier of hydrogenase, is similar in most respects to the multiheme low-potential cytochrome c3 which is characteristic of the genus Desulfovibrio.


Asunto(s)
Grupo Citocromo c/aislamiento & purificación , Aminoácidos/análisis , Grupo Citocromo c/metabolismo , Electroforesis en Gel de Poliacrilamida , Hemo/análisis , Métodos , Peso Molecular , Espectrofotometría
16.
Biochem Biophys Res Commun ; 120(2): 384-9, 1984 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-6329165

RESUMEN

Individual redox potential values are determined for different cytochromes c3. These polyhaemic cytochromes can be divided into two groups: one characterized by only one marked reduction step, the other one giving at least two well-marked reduction steps corresponding to redox potential values ranging from - 0.120 to - 0.400 V. Correlations between potential values and structural data are discussed.


Asunto(s)
Grupo Citocromo c , Fenómenos Químicos , Química , Desulfovibrio/análisis , Oxidación-Reducción , Polarografía
17.
Biochem Biophys Res Commun ; 113(2): 526-30, 1983 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-6307291

RESUMEN

Redox properties of cytochrome c553(550) from Desulfovibrio desulfuricans Norway (Eo' = 0.04 + 0.02 V/NHE) and cytochrome c551 from P. aeruginosa (Eo = 0.25 +/- 0.02 V/NHE) are compared with those of some monohemic c-type cytochromes. The pK value for the equilibrium between the pH-dependent forms of cytochrome c553(550) (pK = 10.3 +/- 0.1) has been also determined. It is to be noted that the difference between redox potentials can extend to nearly 250 mV, though the axial heme ligands are identical. Structural reasons have to be invoked to explain these variations.


Asunto(s)
Proteínas Bacterianas , Grupo Citocromo c/aislamiento & purificación , Desulfovibrio/enzimología , Pseudomonas aeruginosa/enzimología , Fenómenos Químicos , Química , Concentración de Iones de Hidrógeno , Punto Isoeléctrico , Oxidación-Reducción , Espectrofotometría
18.
Biophys Chem ; 17(2): 131-7, 1983 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-6301577

RESUMEN

The effect of urea denaturation on the electroactivity of horse heart cytochrome c has been studied by differential pulse polarography and cyclic voltammetry at a gold electrode; the gold electrode was activated by 4,4'-bipyridine. Essentially, two redox couples with E'01 approximately equal to 0.25 V and E'02 approximately equal to -0.05 V (vs. normal hydrogen electrode) have been detected. The experimental results have been interpreted on the basis of the existence of equilibria between native and denatured electroactive forms; transitory species have been assumed to appear on reduction. The scheme that we have proposed agrees well with the conclusions obtained previously by other authors on conformational changes. Moreover, the advantage of electrochemical techniques in investigating the denaturation process has been underlined.


Asunto(s)
Grupo Citocromo c/metabolismo , Miocardio/metabolismo , Urea/farmacología , Animales , Electroquímica , Caballos , Cinética , Polarografía , Desnaturalización Proteica
20.
Biophys Chem ; 16(1): 51-9, 1982 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-6291659

RESUMEN

The interaction of cytochrome c with a paraffin-wax-impregnated spectroscopic graphite electrode (WISGE) was studied in a medium consisting of 0.1 M potassium phosphate, pH 7.0, by means of differential pulse and cyclic voltammetry. Ferricytochrome c yields on voltammograms a single cathodic peak C around a potential of -0.3 V (vs. Ag/AgCl) and two anodic peaks AI and AII around the potentials of 0.66 and 0.89 V, respectively. Cathodic peak C corresponds to a catalytic reaction during which ferricytochrome c is reduced to ferrocytochrome c: ferricytochrome c is then regenerated by chemical oxidation of ferrocytochrome c by oxygen adsorbed at the WISGE surface. The first, more negative anodic peak AI corresponds to anodic electrochemical oxidation of tyrosine residues, whereas the second, more positive anodic peak (peak AII) corresponds to an anodic reaction of haemin. Voltammetry at a WISGE may provide a valuable technique for obtaining data about cytochrome c properties on electrically charged surface.


Asunto(s)
Grupo Citocromo c/metabolismo , Miocardio/metabolismo , Animales , Electroquímica , Electrodos , Grafito , Caballos , Cinética , Potenciometría , Proteínas/metabolismo
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