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1.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;48(6): 486-492, 06/2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-748219

RESUMEN

The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.


Asunto(s)
Animales , Masculino , Ratones , Expresión Génica , Intrones , Factor II del Crecimiento Similar a la Insulina/genética , MicroARNs , Obstrucción Ureteral/genética , Obstrucción Ureteral/patología , Western Blotting , Citocinas/genética , Modelos Animales de Enfermedad , Fibrosis/genética , Riñón/patología , Reacción en Cadena en Tiempo Real de la Polimerasa , Factores de Tiempo
2.
Braz J Med Biol Res ; 48(6): 486-92, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-25831208

RESUMEN

The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfß, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.


Asunto(s)
Expresión Génica , Factor II del Crecimiento Similar a la Insulina/genética , Intrones , MicroARNs , Obstrucción Ureteral/genética , Obstrucción Ureteral/patología , Animales , Western Blotting , Citocinas/genética , Modelos Animales de Enfermedad , Fibrosis/genética , Riñón/patología , Masculino , Ratones , Reacción en Cadena en Tiempo Real de la Polimerasa , Factores de Tiempo
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