RESUMEN
The hyperpolarization-activated current (I(h)) has been implicated in nociception/pain, but its expression levels in nociceptors remained unknown. We recorded I(h) magnitude and properties by voltage clamp from dorsal root ganglion (DRG) neurons in vivo, after classifying them as nociceptive or low-threshold-mechanoreceptors (LTMs) and as having C-, Aδ- or Aα/ß-conduction velocities (CVs). For both nociceptors andLTMs, I(h) amplitude and I(h) density (at -100 mV) were significantly positively correlated with CV.Median I(h) magnitudes and I(h) density in neuronal subgroupswere respectively:muscle spindle afferents(MSAs):-4.6 nA,-33 pA pF(-1); cutaneous Aα/ß LTMs: -2.2 nA, -20 pA pF(-1); Aß-nociceptors: -2.6 nA, -21 pA pF(-1); both Aδ-LTMs and nociceptors: -1.3 nA, â¼-14 pA pF(-1); C-LTMs: -0.4 nA, -7.6 pA pF(-1); and C-nociceptors: -0.26 nA, -5 pApF(-1). I(h) activation slow time constants (slow τ values) were strongly correlated with fast τ values; both were shortest in MSAs. Most neurons had τ values consistent with HCN1-related I(h); others had τ values closer to HCN1+HCN2 channels, or HCN2 in the presence of cAMP. In contrast, median half-activation voltages (V(0.5)) of -80 to -86 mV for neuronal subgroups suggest contributions of HCN2 to I(h). τ values were unrelated to CV but were inversely correlated with I(h) and I(h) density for all non-MSA LTMs, and for Aδ-nociceptors. From activation curves â¼2-7% of I(h)would be activated at normal membrane potentials. The high I(h) may be important for excitability of A-nociceptors (responsible for sharp/pricking-type pain) and Aα/ß-LTMs (tactile sensations and proprioception). Underlying HCN expression in these subgroups therefore needs to be determined. Altered high I(h) may be important for excitability of A-nociceptors (responsible for sharp/pricking-type pain) and Aα/ß-LTMs (tactile sensations and proprioception). Underlying HCN expression in these subgroups therefore needs to be determined. Altered Ih expression and/or properties (e.g. in chronic/pathological pain states) may influence both nociceptor and LTM excitability.expression and/or properties (e.g. in chronic/pathological pain states) may influence both nociceptor and LTM excitability.
Asunto(s)
Canales Catiónicos Regulados por Nucleótidos Cíclicos/fisiología , Ganglios Espinales/fisiología , Canales Iónicos/fisiología , Canales de Potasio/fisiología , Potenciales de Acción/fisiología , Animales , Femenino , Ganglios Espinales/efectos de los fármacos , Canales Regulados por Nucleótidos Cíclicos Activados por Hiperpolarización , Nociceptores/fisiología , Dolor/fisiopatología , Pirimidinas/farmacología , Ratas , Ratas WistarRESUMEN
Reactive oxygen species (ROS) are produced as by-products of oxidative metabolism and occur in the heart during ischemia and coronary artery reperfusion. The effects of ROS on the electrophysiological properties of intracardiac neurons were investigated in the intracardiac ganglion (ICG) plexus in situ and in dissociated neurons from neonatal and adult rat hearts using the whole-cell patch clamp recording configuration. Bath application of ROS donors, hydrogen peroxide (H(2)O(2)) and tert-butyl hydroperoxide (t-BHP) hyperpolarized, and increased the action potential duration of both neonatal and adult ICG neurons. This action was also recorded in ICG neurons in an adult in situ ganglion preparation. H(2)O(2) and t-BHP also inhibited voltage-gated calcium channel (VGCC) currents and shifted the current-voltage (I-V) relationship to more hyperpolarized potentials. In contrast, H(2)O(2) increased the amplitude of the delayed rectifier K(+) current in neonatal ICG neurons. In neonatal ICG neurons, bath application of either superoxide dismutase (SOD) or catalase, scavengers of ROS, prior to H(2)O(2) attenuated the hyperpolarizing shift but not the inhibition of VGCC by H(2)O(2). In contrast, in adult ICG neurons, application of SOD alone had no effect upon either VGCC current amplitude or the I-V relationship, whereas application of SOD prior to H(2)O(2) exposure abolished both the H(2)O(2)-mediated hyperpolarizing shift and inhibition. These data indicate that ROS alter depolarization-activated Ca(2+) and K(+) conductances which underlie neuronal excitability of ICG neurons. This affects action potential duration and therefore probably modifies autonomic control of the heart during ischemia/reperfusion.
Asunto(s)
Ganglios Autónomos/citología , Neuronas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Potenciales de Acción/efectos de los fármacos , Factores de Edad , Animales , Animales Recién Nacidos , Compuestos de Bario/farmacología , Fenómenos Biofísicos/efectos de los fármacos , Bloqueadores de los Canales de Calcio/farmacología , Cloruros/farmacología , Relación Dosis-Respuesta a Droga , Estimulación Eléctrica , Femenino , Peróxido de Hidrógeno/farmacología , Activación del Canal Iónico/efectos de los fármacos , Miocardio , Neuronas/efectos de los fármacos , Neuronas/fisiología , Oxidantes/farmacología , Técnicas de Placa-Clamp/métodos , Bloqueadores de los Canales de Potasio/farmacología , Ratas , Ratas Wistar , Tetraetilamonio/farmacología , terc-Butilhidroperóxido/farmacologíaRESUMEN
Transient outward currents in rat saphenous arterial myocytes were studied using the perforated configuration of the patch-clamp method. When myocytes were bathed in a Na-gluconate solution containing TEA to block large-conductance Ca2+-activated K+ (BK) currents, depolarizing pulses positive to +20 mV from a holding potential of -100 mV induced fast transient outward currents. The activation and inactivation time constants of the current were voltage dependent, and at +40 mV were 3.6 +/- 0.8 ms and 23.9 +/- 6.4 ms (n = 4), respectively. The steady-state inactivation of the transient outward current was steeply voltage dependent (z = 1.7), with 50% of the current inactivated at -55 mV. The current was insensitive to the A-type K+ channel blocker 4-AP (1-5 mM), and was modulated by external Ca, decreasing to approximately 0.85 of control values upon raising Ca2+ from 1 to 10 mM, and increasing approximately 3-fold upon lowering it to 0.1 mM. Transient outward currents were also recorded following replacement of internal K+ with either Na+ or Cs+, raising the possibility that the current was carried by monovalent ions passing through voltage-gated Ca2+ channels. This hypothesis was supported by the finding that the transient outward current had the same inactivation rate as the inward Ba2+ current, and that both currents were effectively blocked by the L-type Ca2+ channel blocker, nifedipine and enhanced by the agonist BAYK8644.
Asunto(s)
Canales de Calcio/fisiología , Potenciales de la Membrana/fisiología , Miocitos del Músculo Liso/fisiología , Animales , Arterias/fisiopatología , Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Electrofisiología , Técnicas In Vitro , Masculino , Potenciales de la Membrana/efectos de los fármacos , Miocitos del Músculo Liso/efectos de los fármacos , Ratas , Ratas Wistar , Vena Safena , Sensibilidad y EspecificidadRESUMEN
The expression and properties of ionic channels were investigated in dissociated neurons from neonatal and adult rat intracardiac ganglia. Changes in the hyperpolarization-activated and ATP-sensitive K+ conductances during postnatal development and their role in neuronal excitability were examined. The hyperpolarization-activated nonselective cation current, Ih, was observed in all neurons studied and displayed slow time-dependent rectification. An inwardly rectifying K+ current, IK(IR), was present in a population of neurons from adult but not neonatal rats and was sensitive to block by extracellular Ba2+ Using the perforated-patch recording configuration, an ATP-sensitive K+ (KATP) conductance was identified in > or = 50% of intracardiac neurons from adult rats. Levcromakalim evoked membrane hyperpolarization, which was inhibited by the sulphonylurea drugs, glibenclamide and tolbutamide. Exposure to hypoxic conditions also activated a membrane current similar to that induced by levcromakalim and was inhibited by glibenclamide. Changes in the complement of ion channels during postnatal development may underlie observed differences in the function of intracardiac ganglion neurons during maturation. Furthermore, activation of hyperpolarization-activated and KATP channels in mammalian intracardiac neurons may play a role in neural regulation of the mature heart and cardiac function during ischaemia-reperfusion.
Asunto(s)
Envejecimiento/fisiología , Animales Recién Nacidos/fisiología , Sistema de Conducción Cardíaco/fisiología , Neuronas/fisiología , Adenosina Trifosfato/fisiología , Animales , Cationes/metabolismo , Conductividad Eléctrica , Sistema de Conducción Cardíaco/citología , Iones , Potasio/fisiología , Canales de Potasio/fisiología , Canales de Potasio de Rectificación Interna/fisiología , Ratas , Sodio/fisiologíaRESUMEN
The hyperpolarization-activated nonselective cation current, I(h), was investigated in neonatal and adult rat intracardiac neurons. I(h) was observed in all neurons studied and displayed slow time-dependent rectification. I(h) was isolated by blockade with external Cs(+) (2 mM) and was inhibited irreversibly by the bradycardic agent, ZD 7288. Current density of I(h) was approximately twofold greater in neurons from neonatal (-4.1 pA/pF at -130 mV) as compared with adult (-2.3 pA/pF) rats; however, the reversal potential and activation parameters were unchanged. The reversal potential and amplitude of I(h) was sensitive to changes in external Na(+) and K(+) concentrations. An inwardly rectifying K(+) current, I(K(IR)), was also present in intracardiac neurons from adult but not neonatal rats and was blocked by extracellular Ba(2+). I(K(IR)) was present in approximately one-third of the adult intracardiac neurons studied, with a current density of -0.6 pA/pF at -130 mV. I(K(IR)) displayed rapid activation kinetics and no time-dependent rectification consistent with the rapidly activating, inward K(+) rectifier described in other mammalian autonomic neurons. I(K(IR)) was sensitive to changes in external K(+), whereby raising the external K(+) concentration from 3 to 15 mM shifted the reversal potential by approximately +36 mV. Substitution of external Na(+) had no effect on the reversal potential or amplitude of I(K(IR)). I(K(IR)) density increases as a function of postnatal development in a population of rat intracardiac neurons, which together with a concomitant decrease in I(h) may contribute to changes in the modulation of neuronal excitability in adult versus neonatal rat intracardiac ganglia.
Asunto(s)
Ganglios Parasimpáticos/citología , Ganglios Parasimpáticos/crecimiento & desarrollo , Corazón/inervación , Neuronas/fisiología , Factores de Edad , Animales , Animales Recién Nacidos , Cardiotónicos/farmacología , Cesio/farmacología , Conductividad Eléctrica , Técnicas In Vitro , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Técnicas de Placa-Clamp , Potasio/farmacocinética , Pirimidinas/farmacología , Ratas , TemperaturaRESUMEN
The effects of verapamil on the large conductance Ca-activated K (BK) channel from rat aortic smooth muscle cells were examined at the single channel level. Micromolar concentrations of verapamil produced a reversible flickering block of the BK channel activity. Kinetic analysis showed that verapamil decreased markedly the time constants of the open states, without any significant change in the time constants of the closed states. The appearance of an additional closed state-specifically, a nonconducting, open-blocked state--was also observed, whose time constant would reflect the mean residence time of verapamil on the channel. These observations are indicative of a state-dependent, open-channel block mechanism. Dedicated kinetic (group) analysis confirmed the state-dependent block exerted by verapamil. D600 (gallopamil), the methoxy derivative of verapamil, was also tested and found to exert a similar type of block, but with a higher affinity than verapamil. The permanently charged and membrane impermeant verapamil analogue D890 was used to address other important features of verapamil block, such as the sidedness of action and the location of the binding site on the channel protein. D890 induced a flickering block of BK channels similar to that observed with verapamil only when applied to the internal side of the membrane, indicating that D890 binds to a site accessible from the cytoplasmic side. Finally, the voltage dependence of D890 block was assessed. The experimental data fitted with a Langmuir equation incorporating the Woodhull model for charged blockers confirms that the D890-binding site is accessed from the internal mouth of the BK channel, and locates it approximately 40% of the membrane voltage drop along the permeation pathway.
Asunto(s)
Antiarrítmicos/farmacología , Músculo Liso Vascular/citología , Músculo Liso Vascular/efectos de los fármacos , Técnicas de Placa-Clamp , Canales de Potasio Calcio-Activados , Canales de Potasio/metabolismo , Verapamilo/farmacología , Animales , Aorta/anatomía & histología , Sitios de Unión , Galopamilo/análogos & derivados , Galopamilo/farmacología , Cinética , Canales de Potasio de Gran Conductancia Activados por el Calcio , Masculino , Bloqueadores de los Canales de Potasio , Ratas , Ratas WistarRESUMEN
Recordings of membrane potential (Em) and intracellular [Cl-] ([Cl-]i) were made from the smooth muscle of human umbilical and placental arteries, using double-barrelled, ion-sensitive microelectrodes. In both arteries, [Cl-]i was above equilibrium with Em. In the umbilical artery, [Cl-]i was 33.8+/-0.9 mM (+/-SD, n=19) and Em -54.9+/-1.3 mV and in the placental artery respectively 35.1+/-0.7 mM (n=17) and -50.6+/-0.9 mV. In both arteries, [Cl-]i was reduced and Em hyperpolarised significantly by successive additions of 100 microM 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulphonic acid (DIDS), 10 microM bumetanide and 1 mM acetazolamide, thus revealing the presence of Cl-/HCO3- exchange, (Na+K+Cl) cotransport and "pump III". In the presence of all three inhibitors, [Cl-]i was in equilibrium with Em. As in earlier studies on rat arterial smooth and cardiac muscle, pump III was unaffected by DIDS, bumetanide, metolazone and the removal of Na+, partly inhibited by chlorothiazide and fully inhibited by ethacrynic acid. The results are discussed in terms of the possibility that of chloride accumulating systems may regulate vasomotor tone in the foetoplacental unit.
Asunto(s)
Cloruros/metabolismo , Músculo Liso Vascular/metabolismo , Placenta/irrigación sanguínea , Arterias Umbilicales/metabolismo , Ácido 4,4'-Diisotiocianostilbeno-2,2'-Disulfónico/farmacología , Acetazolamida/farmacología , Antiportadores/antagonistas & inhibidores , Antiportadores/metabolismo , Arterias/metabolismo , Transporte Biológico Activo , Bumetanida/farmacología , Proteínas Portadoras/antagonistas & inhibidores , Proteínas Portadoras/metabolismo , Antiportadores de Cloruro-Bicarbonato , Medios de Cultivo , Femenino , Humanos , Microelectrodos , Embarazo , Sodio/administración & dosificación , Simportadores de Cloruro de Sodio-PotasioRESUMEN
Interest in the functions of intracellular chloride expanded about twenty years ago but mostly this referred to tissues other than smooth muscle. On the other hand, accumulation of chloride above equilibrium seems to have been recognised more readily in smooth muscle. Experimental data is used to show by calculation that the Donnan equilibrium cannot account for the chloride distribution in smooth muscle but it can in skeletal muscle. The evidence that chloride is normally above equilibrium in smooth muscle is discussed and comparisons are made with skeletal and cardiac muscle. The accent is on vascular smooth muscle and the mechanisms of accumulation and dissipation. The three mechanisms by which chloride can be accumulated are described with some emphasis on calculating the driving forces, where this is possible. The mechanisms are chloride/bicarbonate exchange, (Na+K+Cl) cotransport and a novel entity, "pump III", known only from own work. Their contributions to chloride accumulation vary and appear to be characteristic of individual smooth muscles. Thus, (Na+K+Cl) always drives chloride inwards, chloride/bicarbonate exchange is always present but does not always do it and "pump III" is not universal. Three quite different biophysical approaches to assessing chloride permeability are considered and the calculations underlying them are worked out fully. Comparisons with other tissues are made to illustrate that low chloride permeability is a feature of smooth muscle. Some of the functions of the high intracellular chloride concentrations are considered. This includes calculations to illustrate its depolarising influence on the membrane potential, a concept which, experience tells us, some people find confusing. The major topic is the role of chloride in the regulation of smooth muscle contractility. Whilst there is strong evidence that the opening of the calcium-dependent chloride channel leads to depolarisation, calcium entry and contraction in some smooth muscles, it appears that chloride serves a different function in others. Thus, although activation and inhibition of (Na+K+Cl) cotransport is associated with contraction and relaxation respectively, the converse association of inhibition and contraction has been seen. Nevertheless, inhibition of chloride/bicarbonate exchange and "pump III" and stimulation of (K+Cl) cotransport can all cause relaxation and this suggests that chloride is always involved in the contraction of smooth muscle. The evidence that (Na+K+Cl) cotransport more active in experimental hypertension is discussed. This is a common but not universal observation. The information comes almost exclusively from work on cultured cells, usually from rat aorta. Nevertheless, work on smooth muscle freshly isolated from hypertensive rats confirms that (Na+K+Cl) cotransport is activated in hypertension but there are several other differences, of which the depolarisation of the membrane potential may be the most important.Finally, a simple calculation is made which indicates as much as 40% of the energy put into the smooth muscle cell membrane by the sodium pump is necessary to drive (Na+K+Cl) cotransport. Notwithstanding the approximations in this calculation, this suggests that chloride accumulation is energetically expensive. Presumably, this is related to the apparently universal role of chloride in contraction.
Asunto(s)
Cloruros/metabolismo , Músculo Liso/metabolismo , Animales , Bicarbonatos/metabolismo , Fenómenos Biofísicos , Biofisica , Proteínas Portadoras/metabolismo , Metabolismo Energético , Concentración de Iones de Hidrógeno , Hipertensión/metabolismo , Técnicas In Vitro , Transporte Iónico , Contracción Muscular/fisiología , Potasio/metabolismo , Ratas , Sodio/metabolismo , Simportadores de Cloruro de Sodio-PotasioRESUMEN
We used the patch-clamp method to characterize the BK channel in freshly isolated myocytes from the saphenous branch of the rat femoral artery. Single-channel recordings revealed that the BK channel had a conductance of 187 pS in symmetrical 150 mM KCl, was blocked by external tetraethylammonium (TEA) with a KD(TEA) of approx. 300 microM at +40 mV, and by submicromolar charybdotoxin (CTX). The sensitivity of the BK channel to Ca was especially high (KD(ca) approx. 0.1 microM at +60 mV) compared to skeletal muscle and neuronal tissues. We also investigated the macroscopic K current, which under certain conditions is essentially sustained by BK channels. This conclusion is based on the findings that the macroscopic current activated upon depolarization follows a single exponential time course and is virtually fully blocked by 100 nM CTX and 5 mM external TEA. We made use of this occurrence to assess the voltage and Ca dependence of the macroscopic BK current. In intact myocytes, the BK channel showed a strong and voltage-dependent reduction of the outward current (62% at +40 mV), most likely due to block by intracellular Ba and polyamines. The results obtained from macroscopic and unitary current indicate that approx. 2.5% of the BK channels are active under physiological conditions, sustaining approx. 20 pA of outward current. Given the high input resistance of these cells, few BK channels are required to open in order to cause a significant membrane hyperpolarization, and thus function to limit the contraction resulting from acute increases in intravascular pressure, or in response to hypertensive pathologies.
Asunto(s)
Calcio/farmacología , Músculo Liso Vascular/fisiología , Canales de Potasio Calcio-Activados , Canales de Potasio/efectos de los fármacos , Canales de Potasio/fisiología , Animales , Presión Sanguínea , Caribdotoxina/farmacología , Conductividad Eléctrica , Arteria Femoral/química , Arteria Femoral/fisiología , Cinética , Canales de Potasio de Gran Conductancia Activados por el Calcio , Masculino , Músculo Liso Vascular/química , Técnicas de Placa-Clamp , Ratas , Ratas Wistar , Tetraetilamonio/farmacología , Resistencia VascularRESUMEN
The inhibition by loop diuretics of K efflux (tracer (86)Rb) from the rat femoral arterial smooth muscle was measured in normotension and in DOCA-salt hypertension. The sensitivity sequence (bumetanide > piretanide > furosemide) was the characteristic pharmacological profile of (Na+K+Cl) cotransport. In hypertension, cotransport activity was 46% greater than in normotension and the sensitivity to loop diuretics was threefold less. Intracellular ¿K and the Na, K and Cl permeability ratios and electrogenic Na pump activity were assessed electrophysiologically in normotension and hypertension. ¿K(i) was lower in hypertension (173 mM) than normotension (198 mM) but the other parameters (P(Na/Cl) = 0.14, P(Cl)/P(K) = 0.19 and electrogenic pump = -8.3 mV in normotension) were not significantly different. Ionic permeabilities to Na, K and Cl were significantly lower in hypertension than normotension. Plasma ¿Na, but not ¿K, was higher in hypertension than normotension. The conclusion is that increased activation of (Na+K+Cl) cotransport in hypertension plays a major role in the elevation of ¿Cl(i) and depolarisation of the membrane potential in vascular smooth muscle in DOCA-salt hypertension. The role of (Na+K+Cl) cotransport in vascular smooth muscle in this model of hypertension is discussed in relation to ¿Cl(i), depolarisation of the membrane potential and contraction and in relation to cell growth.
Asunto(s)
Cloruros/metabolismo , Desoxicorticosterona , Hipertensión/metabolismo , Músculo Liso Vascular/metabolismo , Potasio/metabolismo , Sodio/metabolismo , Animales , Bumetanida/farmacología , Permeabilidad de la Membrana Celular/efectos de los fármacos , Permeabilidad de la Membrana Celular/fisiología , Diuréticos/farmacología , Eritrocitos/química , Furosemida/farmacología , Hipertensión/inducido químicamente , Hipertensión/fisiopatología , Transporte Iónico , Masculino , Plasma/química , Ratas , Ratas Sprague-Dawley , Sulfonamidas/farmacologíaRESUMEN
UNLABELLED: We investigated the effects of L-glutamine (0-20 mM) on cardiac function. The isolated perfused working rat heart (left atrial and aortic pressures of 5 and 70 cm H2O, respectively) was subjected to 20 min of normothermic low-flow ischaemia followed by reperfusion for 35 min. In the absence of glutamine, ischaemia-reperfusion caused an immediate significant (P < 0.01) fall in cardiac output from 46 to 20 ml/min, with a further deterioration to 17 ml/min at 35 min reperfusion. Ischaemia also caused a significant (P < 0.05) fall in myocardial glutamate from 2.6 to 1.8 mumol/g wet weight; and ischaemia-reperfusion caused significant (each P < 0.05) diminutions of myocardial ATP from 3.5 to 1.0 mumol/g wet weight and phosphocreatine from 4.8 to 1.5 mumol/g wet weight and resulted in significant (P < 0.05) accumulation of myocardial lactate from 0.9 to 4.3 mumol/g wet weight. Glutamine, present throughout the perfusion protocol (i.e. prior to ischaemia), at or above 1.25 mM, prevented the post-ischaemic diminution of cardiac output and the deleterious changes in myocardial metabolites. Post-ischaemic treatment with glutamine at 2.5 mM completely prevented the post-ischaemic diminution of cardiac output and restored the myocardial metabolites to normal. CONCLUSIONS: Glutamine may be suitable as a cardioprotective and rescue agent. These effects may be mediated by maintenance of myocardial glutamate, ATP and phosphocreatine: and prevention of lactate accumulation.
Asunto(s)
Glutamina/farmacología , Isquemia Miocárdica/prevención & control , Daño por Reperfusión Miocárdica/prevención & control , Miocardio/metabolismo , Adenosina Trifosfato/metabolismo , Alanina/metabolismo , Animales , Gasto Cardíaco , Glutamatos/metabolismo , Glutaminasa/metabolismo , Glutamina/metabolismo , Lactatos/metabolismo , Masculino , Isquemia Miocárdica/metabolismo , Isquemia Miocárdica/terapia , Fosfocreatina/metabolismo , Ratas , Ratas Sprague-Dawley , Agua/metabolismoRESUMEN
1. Intracellular [Cl-] ([Cl-]i) was measured with ion-selective microelectrodes in rat femoral arterial smooth muscle in normotensive controls and after the induction of deoxycorticosterone acetate/salt hypertension. 2. Linear regression of [Cl-]i and time after the induction of hypertension showed good correlation (r = 0.96) for 5-6 weeks, as [Cl-]i increased from 30 +/- 1 mmol/l (mean +/- SD, n = 16), to 49 +/- 2 mmol/l (n = 9, P < 0.0001). 3. Arterial systolic blood pressure also increased linearly (r = 0.97) for 5-6 weeks as hypertension developed from 122 +/- 1 mmHg (n = 20) to 187 +/- 7 mmHg (n = 14): there was consequently a linear relationship between [Cl-]i and arterial systolic blood pressure (r = 0.96). 4. The increase in [Cl-]i was partly because Na(+)-K(+)-Cl- co-transport activity, estimated from the fall in [Cl-]i caused by bumetanide, was greater in hypertension (18 mmol/l) than in normotension (10 mmol/l). This finding, and the depolarization of the membrane potential in hypertension (-56 +/- 3 mV compared with -64 +/- 4 mV in normotension; P < 0.0001), confirms previous studies. 5. The increase in [Cl-]i was also partly due to greater activity of an Na(+)- and HCO3(-)-independent, acetazolamide-sensitive inward Cl- transport system; thus acetazolamide reduced [Cl-]i by 7 mmol/l in normotension and by 16 mmol/l in hypertension. 6. In Cl(-)-free media, the membrane potential in normotension (-59 +/- 5 mV) was not significantly different from that in hypertension (-60 +/- 4 mV). 7. The role of [Cl-]i in the depolarization of the membrane potential in hypertension is discussed.
Asunto(s)
Canales de Cloruro/metabolismo , Hipertensión/metabolismo , Músculo Liso Vascular/metabolismo , Acetazolamida/farmacología , Animales , Aniones , Bumetanida/farmacología , Canales de Cloruro/efectos de los fármacos , Desoxicorticosterona , Diuréticos/farmacología , Masculino , Potenciales de la Membrana , Microelectrodos , Músculo Liso Vascular/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Cloruro de Sodio , Estadísticas no ParamétricasRESUMEN
The electrical characteristics of isolated neonatal rat intracardiac neurons were examined at 22 and 37 degrees C using the perforated-patch whole cell recording technique. The mean resting membrane potential was -52.0 mV at 37 degrees C and exhibited no temperature dependence. Lowering the temperature from 37 to 22 degrees C decreased the mean input resistance from 854 to 345 Momega, respectively, and reduced the membrane time constant approximately threefold yielding a Q10 of 2.1. Hyperpolarizing current pulses induced time-dependent rectification of the voltage response in all neurons at both temperatures. This behavior was previously not observed in dialyzed neurons and was reversibly blocked by external Cs+ (2 mM) but not Ba2+ (1 mM). Voltage-clamp studies of isolated neurons revealed a hyperpolarization-activated inward current. This inwardly rectifying conductance was isolated from other membrane currents using external Cs+. The time and voltage dependence of this current is consistent with Ih and contributes to the passive electrical properties of rat intracardiac neurons. In >90% of the neurons studied, depolarizing currents evoked firing of multiple, adapting, action potentials at 22 degrees C. The number of action potentials increased with current strength producing a mean discharge of 5.1 (+100 pA, 1 s pulse), which was attenuated at 37 degrees C to a mean of 1.4. The amplitude and kinetics of the slow, muscarine-sensitive inward and outward currents (IM) were highly temperature dependent. Lowering the temperature from 37 to 22 degrees C reduced the steady-state current amplitude by approximately one-third and the rate of deactivation of IM by six- to ninefold at all voltages examined. The average Q10 for the time constant of deactivation of IM was 3.7 +/- 0.3 (mean +/- SE). Acetylcholine (ACh) induced tonic discharges in response to depolarizing currents (+100 pA, 1 s pulse) at both temperatures. This effect of ACh was inhibited by the muscarinic receptor antagonists, pirenzepine (100 nM), and mL-toxin (60 nM). At 37 degrees C, a mean discharge of 1.5 was increased to 23.5 in the presence of ACh. A similar switch from phasic to tonic discharge was also produced by the potassium channel inhibitors, Ba2+ (1 mM) and uridine-5'-triphosphate (UTP; 100 microM), whereas cadmium, 4-aminopyridine, apamin, charybdotoxin, and dendrotoxin did not alter discharge activity. The pharmacological sensitivity profile and temperature dependence of the active membrane properties are consistent with the muscarine-sensitive potassium current (IM) regulating the discharge activity in rat intracardiac neurons.
Asunto(s)
Cationes/farmacología , Corazón/efectos de los fármacos , Potenciales de la Membrana/efectos de los fármacos , Neuronas/fisiología , Sistema Nervioso Parasimpático/efectos de los fármacos , Acetilcolina/farmacología , Animales , Bario/farmacología , Células Cultivadas , Potenciales de la Membrana/fisiología , Canales de Potasio/efectos de los fármacos , RatasRESUMEN
The intracellular Cl concentration ([Cl]i) in rat cardiac ventricular muscle, measured with double-barreled microelectrodes in vitro, was 21.3 +/- 1.5 (SD) mM [number of observations (n) = 46]. With the Na-K-Cl cotransport inhibitor bumetanide (10 microM), it fell to 13.4 +/- 1.4 mM (n = 27), and with 1 mM acetazolamide, it fell further, to 7.2 +/- 1.5 mM (n = 5), close to equilibrium with the membrane potential. In the absence of Na, [Cl]i was 15.9 +/- 1.4 mM (n = 8), and with 1 mM acetazolamide, it fell to 6.5 +/- 0.6 mM (n = 4), again close to equilibrium. The bumetanide- and Na-insensitive components of inward Cl pumping were inhibited by chlorothiazide and ethacrynic acid but were unaffected by the Na-Cl cotransport inhibitor metolazone. There was inhibition of Na-K-Cl cotransport by chlorothiazide = acetazolamide > metolazone. The anion exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid and HCO3 had no effect on [Cl]i in any condition. Thus Cl accumulation in the rat ventricle is fully accounted for by two systems, namely, Na-K-Cl cotransport and an Na-independent, possibly primary active, process.
Asunto(s)
Cloruros/metabolismo , Miocardio/metabolismo , Sodio/fisiología , Simportadores , Acetazolamida/farmacología , Animales , Aniones/metabolismo , Proteínas Portadoras/antagonistas & inhibidores , Ácido Etacrínico/farmacología , Ventrículos Cardíacos , Membranas Intracelulares/efectos de los fármacos , Membranas Intracelulares/metabolismo , Intercambio Iónico , Masculino , Potenciales de la Membrana , Miocardio/citología , Concentración Osmolar , Ratas , Ratas Sprague-Dawley , Sodio/farmacología , Simportadores del Cloruro de Sodio , Función Ventricular/efectos de los fármacosRESUMEN
We have investigated the neutral and basic amino acid transporter (NBAT)-induced transport of L-histidine in Xenopus laevis oocytes. Transport of L-histidine (pH 7.5) was electrogenic and Na+-dependent with a 14-fold increase in L-histidine- (1 mM) evoked current (I(His) = -14.7 +/- 1.5 nA) in NBAT-expressing oocytes compared with native (water-injected or uninjected) oocytes (-1.0 +/- 0.2 nA); the Na+-dependent histidine transport showed a stoichiometry of 1:1 (histidine:sodium). I(His) was stereospecific at pH 7.5 and saturable in both NaCl and tetramethylammonium chloride media. L-Histidine (1 mM) at pH 8.5, at which histidine is uncharged, evoked an Na+-independent outward current (11 +/- 1.2 nA) in NBAT-expressing oocytes. The total inward 0.1 mM I(His) increased from -9 +/- 0.8 nA at pH 7.5 to -19 +/- 2.6 nA at pH 6.5, at which histidine is predominantly cationic. The increase in I(His) from pH 7.5 to 6.5 was found to be almost entirely due to the Na+-independent component. At pH 7.5, L-histidine weakly inhibited the Na+-independent L-arginine uptake; however, this inhibition was much stronger (>90%) at pH 6.5. L-Histidine transport, at pH 7.5, is stimulated by NBAT expression, but unlike L-phenylalanine or L-arginine transport, L-histidine transport is Na+-dependent and stereoselective. The induction of Na+-dependent L-histidine transport in NBAT-expressing oocytes provides new evidence that NBAT stimulates functionally distinct amino acid transporters including Na+-dependent L-histidine and Na+-independent L-arginine and L-phenylalanine transporters. The parallel induction of two different mechanisms argues that NBAT is not an amino acid transporter itself but, instead, is a transport-activating protein for a range of amino acid translocases.
Asunto(s)
Sistemas de Transporte de Aminoácidos Básicos , Sistemas de Transporte de Aminoácidos Neutros , Aminoácidos/fisiología , Proteínas Portadoras/fisiología , Histidina/metabolismo , Animales , Arginina/metabolismo , Transporte Biológico , Conductividad Eléctrica , Concentración de Iones de Hidrógeno , Potenciales de la Membrana , Oocitos , Técnicas de Placa-Clamp , Proteínas Recombinantes , Sodio/fisiología , Estereoisomerismo , Xenopus laevisRESUMEN
1. Double-barrelled ion-selective microelectrodes were used to examine the effects of exogenous noradrenaline upon the membrane potential (Em) and intracellular chloride concentration ([Cl]i) of arterial smooth muscle from the saphenous branch of the femoral artery of the rat. 2. After treatment with 0.6 mM 6-hydroxydopamine (to functionally denervate the tissue), exogenous noradrenaline (5 nM) caused repeatable depolarization of Em from -63.7 +/- 2.4 mV (s.d., n = 18) to -53.8 +/- 3.4 mV (P < 0.0001) and increases in [Cl]i from 31.0 +/- 0.5 mM to 42.5 +/- 2.2 mM (P < 0.0001). 3. In the presence of 10 microM bumetanide (an inhibitor of (Na-K-Cl) cotransport), 5 nM noradrenaline caused a depolarization of Em of 3.0 +/- 3.2 mV, and a rise in [Cl]i of 4.5 +/- 2.5 mM. 4. In the presence of bumetanide and 1 mM acetazolamide (used as an inhibitor of a Na-independent inward Cl pump), noradrenaline had no effect on Em or [Cl]i. 5. In the absence of extracellular chloride, the rise in apparent [Cl]i in response to 5 nM noradrenaline was abolished but there was a depolarization of 2.0 +/- 3.9 mV. 6. These results are consistent with the stimulation of (Na-K-Cl) cotransport and a Na-independent Cl pump by exogenous noradrenaline and with the consequent increase in [Cl]i and shift in ECl potentiating the depolarization caused by noradrenaline. The possibility that modulation of [Cl]i may be a general mechanism of Em regulation is discussed.
Asunto(s)
Cloruros/metabolismo , Músculo Liso Vascular/efectos de los fármacos , Norepinefrina/farmacología , Acetazolamida/farmacología , Animales , Bumetanida/farmacología , Proteínas Portadoras/antagonistas & inhibidores , Proteínas Portadoras/metabolismo , Diuréticos/farmacología , Arteria Femoral , Técnicas In Vitro , Masculino , Potenciales de la Membrana/efectos de los fármacos , Músculo Liso Vascular/metabolismo , Músculo Liso Vascular/fisiología , Ratas , Ratas Sprague-Dawley , Simportadores de Cloruro de Sodio-PotasioRESUMEN
Monophasic compound action potentials were recorded from Rana sciatic nerves. Three distinct peaks were observed and designated A alpha, A delta and C. All peaks were abolished by replacement of the external medium with Na(+)-free solution. However, the C peak alone was unaffected by external application of 1 microM tetrodotoxin (TTX), both A peaks were completely suppressed. The C peak was also the most resistant to chronic depolarization caused by increased external K+. K+ (17.6 mM) solution reduced peak areas to 5 +/- 4, 27 +/- 11 and 63 +/- 14% of control for A alpha, A delta and C components. The C peak was therefore Na(+)-dependent, TTX-resistant and K(+)-depolarization resistant. These attributes are similar to those described for somatal TTX-resistant Na+ channels in other species. But, application of 1 microM TTX to a K(+)-depolarized nerve caused a further reduction in C peak area, suggestive of a voltage-dependent block by TTX similar to that reported for cardiac muscle Na+ channels.
Asunto(s)
Potasio/farmacología , Nervio Ciático/efectos de los fármacos , Nervio Ciático/fisiología , Tetrodotoxina/fisiología , Potenciales de Acción/efectos de los fármacos , Animales , Electrofisiología , Fibras Nerviosas/fisiología , Fibras Nerviosas Mielínicas/fisiología , Rana pipiens , Rana temporaria , Sodio/farmacologíaRESUMEN
Merkel cells in human buccal mucosa and hard palate possess the carbonic anhydrase I isoenzyme (CAI). CAI colocalized immunocytochemically with a range of Merkel cell cytokeratins, namely CK 7, 8, 18, 19 and 20. No other cells in the oral epithelium were immunoreactive for the CAI antibody. The presence of the enzyme may be related to the function of sensory receptors that produce a sustained response to a maintained mechanical stimulus.
Asunto(s)
Anhidrasas Carbónicas/análisis , Células de Merkel/enzimología , Mucosa Bucal/citología , Mucosa Bucal/enzimología , Adulto , Anciano , Femenino , Humanos , Inmunohistoquímica , Queratinocitos/enzimología , Masculino , Persona de Mediana EdadRESUMEN
The chronotropic actions of Na+, K+, Cl- cotransport were investigated by studying the effects of the loop diuretics bumetanide and furosemide, specific inhibitors of the cotransporter, on an isolated rat sino-atrial node preparation. Application of bumetanide decreased the cycle length from 0.334 s (+/- 0.087 S.D.) to 0.279 s (+/- 0.083, n = 16, P = 6.5 x 10(-6)) in Hepes-buffered physiological salt solution (PSS). Similar decreases were recorded in bicarbonate-buffered PSS. Chloride channel blockers indicate that the tachycardia evoked by loop diuretics is not due their blocking of chloride channels. Thus, 4,4'-dinitrostilbene-2,2'-disulphonic acid (DNDS) and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) had a negative chronotropic action and 2-[(2-cyclopentyl-6,7-dichloro-2,3-dihydro-2-methyl-1-oxo-1H-inden -5-yl) oxy] acetic acid (IAA-94) produced no change in cycle length. Pharmacological manoeuvres indicate that the positive chronotropic action of loop diuretics is associated with catecholamine release. The positive chronotropic action of bumetanide was inhibited by the beta-adrenoceptor antagonists, propranolol and atenolol, but was unaffected by atropine.
Asunto(s)
Bumetanida/farmacología , Proteínas Portadoras/antagonistas & inhibidores , Cloruros/metabolismo , Diuréticos/farmacología , Frecuencia Cardíaca/efectos de los fármacos , Potasio/metabolismo , Sodio/metabolismo , Animales , Proteínas Portadoras/fisiología , Masculino , Ratas , Ratas Sprague-Dawley , Nodo Sinoatrial/efectos de los fármacos , Nodo Sinoatrial/fisiología , Simportadores de Cloruro de Sodio-PotasioRESUMEN
To elucidate the electrical events associated with the movement of amino acids by the neutral and basic amino acid transporter (NBAT)-encoded protein (Yan, N., Mosckovitz, R., Gerber, L.D., Mathew, S., Murty, V.V. V.S., Tate, S.S., and Udenfriend, S. (1994) Proc. Natl. Acad. Sci. USA 91, 7548-7552), we have investigated the membrane potential and current changes associated with the increased transport of amino acids across the cell membrane of NBAT cRNA-injected Xenopus laevis oocytes. Superfusion of 0.05 mM L-phenylalanine, in current-clamped NBAT-injected oocytes, caused a hyperpolarization (8.5 +/- 0.9 mV), but superfusion of L-arginine caused a depolarization (18.3 +/- 1.3 mV). In voltage-clamped (-60 mV) oocytes, superfusion of L-phenylalanine evoked a sodium- and chloride-independent, saturable (Km = 0.34 +/- 0.02 mM, Imax = 31.3 +/- 0.5 nA), outward current. This outward current was reduced in the presence of high external [K] and was barium-sensitive. Outward currents were also evoked by L-leucine, L-glutamine, L-alanine, D-phenylalanine, and L-beta-phenylalanine. Superfusion of L-arginine evoked a saturable (Km = 0.09 +/- 0.02 mM, Imax = -29.2 +/- 1.3 nA) inward current; L-lysine and D-arginine also evoked inward currents. L-Glutamate and beta-alanine failed to evoke any currents. Effluxes of L-[3H]phenylalanine and L-[3H]arginine were trans-stimulated in the presence of either amino acid. Flux-current comparisons indicated amino acid:charge movement stoichiometry of 1:1 for both neutral and cationic amino acids. These findings indicate that the amino acid transport activity(ies) expressed in NBAT cRNA-injected oocytes is electrogenic by a mechanism including the outward movement of a net positive charge (potassium ion or cationic amino acid) in exchange for uptake of a neutral amino acid.