RESUMEN
The human gene AGTRL1 is an angiotensin II receptor-like gene expressed in vasculature, which acts as the receptor for the small peptide APELIN, and a co-receptor for Human Immunodeficiency Virus. Mammalian AGTRL1 has been shown to modulate cardiac contractility, venous and arterial dilation, and endothelial cell migration in vitro, but no role in the development of the vasculature, or other tissues, has been described. We report the identification and expression of the zebrafish ortholog of the human gene AGTRL1. Zebrafish agtrl1a is first expressed before epiboly in dorsal precursors. During epiboly it is expressed in the enveloping layer, yolk syncytial layer and migrating mesendoderm. During segmentation stages, expression is observed in epithelial structures such as adaxial cells, border cells of the newly formed somites, developing lens, otic vesicles and venous vasculature.
Asunto(s)
Epitelio/embriología , Regulación del Desarrollo de la Expresión Génica , Receptor de Angiotensina Tipo 1/genética , Proteínas de Pez Cebra/genética , Pez Cebra/embriología , Secuencia de Aminoácidos , Animales , Blástula/metabolismo , Vasos Sanguíneos/embriología , Vasos Sanguíneos/metabolismo , Fase de Segmentación del Huevo/metabolismo , Clonación Molecular , Embrión no Mamífero/metabolismo , Epitelio/metabolismo , Gástrula/metabolismo , Humanos , Hibridación in Situ , Mesodermo/metabolismo , Datos de Secuencia Molecular , Filogenia , Alineación de Secuencia , Somitos/citología , Somitos/metabolismo , Pez Cebra/anatomía & histología , Pez Cebra/genéticaRESUMEN
In the honey bee, an eminently fertile queen with up to 200 ovarioles per ovary monopolizes colony level reproduction. In contrast, worker bees have only few ovarioles and are essentially sterile. This phenotype divergence is a result of caste-specifically modulated juvenile hormone and ecdysteroid titers in larval development. In this study we employed a differential-display reverse transcription (DDRT)-PCR protocol to detect ecdysteroid-regulated gene expression during a critical phase of caste development. We identified a Ftz-F1 homolog and a Cut-like transcript. Ftz-F1 could be a putative element of the metamorphic ecdysone response cascade of bees, whereas Cut-like proteins are described as transcription factors involved in maintaining cellular differentiation states. The downregulation of both factors can be interpreted as steps in the metamorphic degradation of ovarioles in worker-bee ovaries.