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1.
Presse Med ; 34(15): 1082-3, 2005 Sep 10.
Artículo en Francés | MEDLINE | ID: mdl-16334886

RESUMEN

INTRODUCTION: Primary epidural lymphoma is generally manifested by isolated epidural involvement. On rare occasions it can lead to cauda equina syndrome. CASE: A 56-year-old man was hospitalized for lumbago, weakness in the legs and sphincter problems. A dorsolumbar MRI revealed that the anterior and posterior peridural space from D11 to L3 was filled by a mass compressing the marrow and compacting the roots. The L2 to L4 laminectomy revealed an extradural tissue leading to the diagnosis of large B-cell lymphoma. Metastatic workup demonstrated unremarkable findings. DISCUSSION: Primary epidural lymphoma accounts for less than 10% of epidural tumours and less than 1% of non-Hodgkin lymphomas. Its clinical manifestations are related to the tumoral process or involvement of medullar vessels. It can be, but rarely is, manifested by cauda equina syndrome. Diagnosis is based on histological analysis of tissue taken during the decompression laminectomy. Prognosis is relatively good.


Asunto(s)
Neoplasias Epidurales/complicaciones , Linfoma de Células B/complicaciones , Polirradiculopatía/etiología , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Terapia Combinada , Ciclofosfamida/uso terapéutico , Doxorrubicina/uso terapéutico , Neoplasias Epidurales/diagnóstico , Neoplasias Epidurales/tratamiento farmacológico , Neoplasias Epidurales/radioterapia , Humanos , Laminectomía , Vértebras Lumbares , Linfoma de Células B/diagnóstico , Linfoma de Células B/tratamiento farmacológico , Linfoma de Células B/radioterapia , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Polirradiculopatía/diagnóstico , Prednisona/uso terapéutico , Pronóstico , Dosificación Radioterapéutica , Inducción de Remisión , Vincristina/uso terapéutico
2.
J Bacteriol ; 181(16): 4961-8, 1999 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10438768

RESUMEN

We have developed a method for identifying essential genes by using an in vitro transposition system, with a small (975 bp) insertional element containing an antibiotic resistance cassette, and mapping these inserts relative to the deduced open reading frames of Haemophilus influenzae by PCR and Southern analysis. Putative essential genes are identified by two methods: mutation exclusion or zero time analysis. Mutation exclusion consists of growing an insertional library and identifying open reading frames that do not contain insertional elements: in a growing population of bacteria, insertions in essential genes are excluded. Zero time analysis consists of monitoring the fate of individual insertions after transformation in a growing culture: the loss of inserts in essential genes is observed over time. Both methods of analysis permit the identification of genes required for bacterial survival. Details of the mutant library construction and the mapping strategy, examples of mutant exclusion, and zero time analysis are presented.


Asunto(s)
Mapeo Cromosómico/métodos , Genoma Bacteriano , Haemophilus influenzae/genética , Southern Blotting , Cromosomas Bacterianos , Cartilla de ADN , Elementos Transponibles de ADN , ADN Bacteriano/análisis , Biblioteca de Genes , Genes Bacterianos , Mutagénesis Insercional , Reacción en Cadena de la Polimerasa , Transformación Genética
3.
Appl Microbiol Biotechnol ; 47(4): 398-404, 1997 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-9163954

RESUMEN

A search for an abundant and economical source of isoflavones, particularly genistein, led to the discovery that the erythromycin-producing organism Saccharopolyspora erythraea also produces this promising new cancer-prevention agent. Erythromycin fermentation is a large-scale, soybean-based process used world-wide for the commercial production of this medically important antibiotic. Results from this study indicate that genistin (the glucoside form of genistein), which is added to the fermentation in the soybean media, was converted to genistein through the action of a beta-glucosidase produced by the organism. Genistein was co-extracted with erythromycin from the fermentation broth, then separated from erythromycin during the second step of the purification process for the production of erythromycin.


Asunto(s)
Isoflavonas/aislamiento & purificación , Isoflavonas/metabolismo , Saccharopolyspora/metabolismo , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada , Medios de Cultivo/metabolismo , ADN-Topoisomerasas de Tipo II/metabolismo , Electroforesis en Gel de Agar , Eritromicina/biosíntesis , Fermentación , Genisteína , Isoflavonas/química , Espectrometría de Masas , Estructura Molecular , Glycine max/metabolismo , beta-Glucosidasa/metabolismo
4.
Phys Med Biol ; 35(6): 703-15, 1990 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-2195559

RESUMEN

This study shows how two layers which are superposed in two low angle x-ray projections can be reconstructed separately without having any a priori knowledge of their shape. Similar to stereoscopic imaging, the projections of the two layers appear at different positions in the two superposition images. Matching one of the layers allows its elimination by subtraction. The resulting difference signal is the so-called 'movement artefact' of the second, mismatched layer. A filter method is developed to reconstruct this layer from the spectrum of its artefact within the Fourier domain. The second layer is reconstructed in an analogous way. So, for example, the bones of the left and the right side of the human skull can be imaged separately. This is used to reduce image artefacts caused by patient movements in subtraction angiography and tumourfluoroscopy of the brain.


Asunto(s)
Procesamiento de Imagen Asistido por Computador , Cráneo/diagnóstico por imagen , Humanos , Radiografía , Técnica de Sustracción
5.
J Bacteriol ; 172(4): 1749-61, 1990 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-2156801

RESUMEN

The Rhodobacter sphaeroides genome contains two unlinked genetic regions each encoding numerous proteins involved in CO2 fixation which include phosphoribulokinases (prkA and prkB), ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcLS and rbcR) (P. L. Hallenbeck and S. Kaplan, Photosynth. Res. 19:63-71, 1988; F. R. Tabita, Microbiol. Rev. 52:155-189, 1988), and two open reading frames linked to rbcLS and rbcR, namely, cfxA and cfxB, respectively (P. L. Hallenbeck, R. Lerchen, P. Hessler, and S. Kaplan, J. Bacteriol. 172:1736-1748). In this study, we examined the unique role(s) of each phosphoribulokinase activity in the regulation of CO2 fixation. Strains were constructed which contain null mutations in prkA and/or prkB. Studies utilizing these strains suggested that CO2 fixation plays an essential role in attaining the cellular redox balance necessary for photoheterotrophic growth. The presence of an external electron acceptor can negate the requirement for CO2 for photoheterotrophic growth. Each form of phosphoribulokinase and ribulose 1,5-bisphosphate carboxylase/oxygenase was shown to have distinct roles in CO2 metabolism when cells were exposed to extremes in CO2 levels. Evidence is also presented which unequivocally demonstrated that regulation of the expression of the enzymes involved in CO2 metabolism is effective at the transcriptional level. Although the two regions of the DNA involved in CO2 fixation are physically unlinked, each region of the DNA can have a profound effect on the expression of the other region of the DNA.


Asunto(s)
Dióxido de Carbono/metabolismo , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos , Fosfotransferasas (Aceptor de Grupo Alcohol) , Fosfotransferasas/genética , Fotosíntesis , Rhodobacter sphaeroides/genética , Cromosomas Bacterianos , Escherichia coli/genética , Prueba de Complementación Genética , Vectores Genéticos , Cinética , Mutación , Fosfotransferasas/metabolismo , Plásmidos , Mapeo Restrictivo , Rhodobacter sphaeroides/enzimología , Rhodobacter sphaeroides/crecimiento & desarrollo , Ribulosa-Bifosfato Carboxilasa/genética , Transcripción Genética
6.
J Bacteriol ; 172(4): 1736-48, 1990 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-2108123

RESUMEN

The Rhodobacter sphaeroides genome contains two unlinked genetic regions each encoding a series of proteins involved in CO2 fixation which include phosphoribulokinase (prkA and prkB) and ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcLS and rbcR) (P. L. Hallenbeck and S. Kaplan, Photosynth. Res. 19:63-71, 1988; F. R. Tabita, Microbiol. Rev. 52:155-189, 1988). We examined the effect of CO2 in the presence and absence of an alternate electron acceptor, dimethyl sulfoxide, on the expression of rbcR and rbcLS in photoheterotrophically grown R. sphaeroides. The expression of both rbcR and rbcLS was shown to depend on the CO2 concentration when succinate was used as the carbon source. It was also demonstrated that CO2 fixation is critical for photoheterotrophic growth but could be replaced by the alternative reduction of dimethyl sulfoxide to dimethyl sulfide. Dimethyl sulfoxide severely depressed both rbcR and rbcLS expression in cells grown photoheterotrophically at CO2 concentrations of 0.05% or greater. However, cells grown photoheterotrophically in the absence of exogenous CO2 but in the presence of dimethyl sulfoxide had intermediate levels of expression of rbcL and rbcR, suggesting partially independent control by limiting CO2 tension. We also present evidence for the existence of two gene products, namely, CfxA and CfxB, which are encoded by genes immediately upstream of rbcLS and rbcR, respectively. Strains were constructed which contained null mutations in cfxA and/or cfxB. Each mutation eliminated expression of the linked downstream rbc operon. Further, studies utilizing these strains demonstrated that each form of ribulose 1,5-bisphosphate carboxylase/oxygenase plays an essential role in maintaining the cellular redox balance during photoheterotrophic growth at differing CO2 concentrations.


Asunto(s)
Regulación Bacteriana de la Expresión Génica , Rhodobacter sphaeroides/genética , Ribulosa-Bifosfato Carboxilasa/genética , Dióxido de Carbono/farmacología , Cromosomas Bacterianos , Clonación Molecular , Dimetilsulfóxido/farmacología , Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Genes Bacterianos , Plásmidos , Mapeo Restrictivo , Rhodobacter sphaeroides/enzimología , Rhodobacter sphaeroides/crecimiento & desarrollo , Ribulosa-Bifosfato Carboxilasa/metabolismo
8.
AJR Am J Roentgenol ; 136(3): 517-20, 1981 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-6781288

RESUMEN

Recent advances in the imaging capabilities of gray scale sonography have increased the accuracy with which gallstones may be diagnosed. Since the sonographic diagnosis of gallstones is often followed by surgery without further confirmatory studies, the avoidance of false-positive diagnoses assumes major importance. In an attempt to improve diagnostic accuracy, 420 gallbladder sonograms were evaluated for gallstones. Positive diagnoses were limited to cases in which the gallbladder was well visualized and contained densities that produced acoustic shadowing or moved rapidly with changes in position. Gallstones were diagnosed in 123 cases and surgery or autopsy in 70 of these patients confirmed stones in 69. There was one false-positive, an accuracy rate for positive diagnosis of 98.6%. Five cases were called indeterminate for stones; one of these had tiny 1 mm stones at surgery. The other four cases had no surgery. Of 276 cases called negative for stones. None of the 146 cases with negative sonograms and oral cholecystography or intravenous cholangiography had stones diagnosed by these methods. Because of its ease and simplicity, sonography is attractive as the initial study in patients suspected of having gallstones. With the criteria used here, a diagnosis of gallstones in the gallbladder can be offered with great confidence.


Asunto(s)
Colelitiasis/diagnóstico , Ultrasonografía , Errores Diagnósticos , Humanos
9.
Urol Radiol ; 3(2): 117-20, 1981.
Artículo en Inglés | MEDLINE | ID: mdl-7314322

RESUMEN

Renal adenocarcinoma is rare before age 20. Clinically and radiographically, it presents similarly in adults and children. Prognosis is related to the clinical stage of the tumor at surgery. Treatment methods are still under investigation.


Asunto(s)
Adenocarcinoma/diagnóstico , Neoplasias Renales/diagnóstico , Adenocarcinoma/diagnóstico por imagen , Adenocarcinoma/patología , Adulto , Factores de Edad , Calcinosis/patología , Femenino , Humanos , Neoplasias Renales/diagnóstico por imagen , Neoplasias Renales/patología , Radiografía , Ultrasonografía
10.
Gastrointest Radiol ; 2(4): 355-7, 1978 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-669157

RESUMEN

Duodenal villous adenomas are premalignant lesions. Prior to malignant transformation, their appearance on upper gastrointestinal examination is often characteristic. Following malignant change, the final diagnosis frequently requires tissue.


Asunto(s)
Adenocarcinoma/diagnóstico por imagen , Adenoma/diagnóstico por imagen , Neoplasias Duodenales/diagnóstico por imagen , Duodeno/diagnóstico por imagen , Neoplasias Primarias Múltiples/diagnóstico por imagen , Anciano , Femenino , Humanos , Radiografía
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