RESUMEN
The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique.
Asunto(s)
L-Lactato Deshidrogenasa/análisis , Cromatografía Líquida de Alta Presión/instrumentación , Cromatografía Líquida de Alta Presión/métodos , Humanos , Isoenzimas , L-Lactato Deshidrogenasa/sangre , L-Lactato Deshidrogenasa/aislamiento & purificación , Miocardio/enzimologíaRESUMEN
We have applied a comprehensive theory of photometric errors to the design and evaluation of an inexpensive stabilized photometer. The photometer is described in terms of a group of modules, the characteristics of which are described in terms of their effect on specific error coefficients. Procedures described in this paper show how the principles described earlier [Clin, Chem. 20, 1028 (1974)] can be used to optimize the design of new instrumentation. Chemical data are included to verify the agreement between predicted and experimental results.
