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Anomalías Múltiples/genética , Cardiopatías Congénitas/patología , Deformidades Congénitas de las Extremidades/patología , Proteínas de Dominio T Box/genética , Células 3T3 , Anomalías Múltiples/patología , Transporte Activo de Núcleo Celular/genética , Animales , Factor Natriurético Atrial/genética , Secuencia de Bases , Línea Celular , Núcleo Celular/metabolismo , Codón sin Sentido , ADN/química , ADN/genética , ADN/metabolismo , Análisis Mutacional de ADN , Salud de la Familia , Femenino , Humanos , Luciferasas/genética , Luciferasas/metabolismo , Masculino , Ratones , Datos de Secuencia Molecular , Mutación , Linaje , Regiones Promotoras Genéticas , Unión Proteica/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Eliminación de Secuencia , Síndrome , Proteínas de Dominio T Box/metabolismoAsunto(s)
Bloqueo Cardíaco/diagnóstico , Prednisolona/análogos & derivados , Recuperación de la Función , Sarcoidosis/diagnóstico , Tomografía Computarizada de Emisión , Adulto , Enfermedad Crónica , Electrocardiografía , Fluorodesoxiglucosa F18 , Granuloma/patología , Corazón/diagnóstico por imagen , Bloqueo Cardíaco/etiología , Bloqueo Cardíaco/terapia , Humanos , Ganglios Linfáticos/patología , Masculino , Radioisótopos de Nitrógeno , Valor Predictivo de las Pruebas , Prednisolona/uso terapéutico , Recuperación de la Función/efectos de los fármacos , Sarcoidosis/complicaciones , Sarcoidosis/patología , Radioisótopos de TalioRESUMEN
Increasing evidence has suggested that mitogen-activated protein kinases (MAPKs) play important roles in the development of cardiac hypertrophy. We and others have reported that the activity of MAPKs is tightly regulated by angiotensin II (Ang II) in cardiac myocytes. In the present study, we determined the molecular mechanism of Ang II-induced inactivation of MAPKs in rat neonatal cardiac myocytes. Ang II increased MAPK phosphatase 1 (MKP-1) gene expressions within 10 min. Levels of MKP-1 transcripts peaked at 30 min and gradually decreased thereafter. The increase in MKP-1 mRNA levels was Ang II-concentration dependent. An Ang II type 1 receptor (AT1)-specific antagonist, CV-11974, completely suppressed the Ang II-induced increase in MKP-1 gene expression, while a type 2 receptor (AT2)-specific antagonist, PD-123319, had no significant effects. Induction of MKP-1 gene expressions by Ang II was inhibited by pretreatment with an intracellular Ca2+ chelator, BAPTA-AM, or with the protein kinase C inhibitors, H-7 and Calphostin C. Phorbol ester and Ca2+ ionophore both significantly increased MKP-1 mRNA levels and showed synergistic action. Overexpression of MKP-1 cDNA blocked the Ang II-induced increase in expressions of immediate early response genes. In addition, Ang II-induced MAPK activation was significantly inhibited by pretreatment with CV-11974, but significantly enhanced by pretreatment with PD-123319. Addition of the AT2 agonist, CGP42112A, reduced basal MAPK activities, and pretreatment with PD-123319 abolished MAPK inactivation by CGP42112A. In conclusion, these observations suggest that Ang II negatively regulates MAPKs through AT1 receptors by increasing MKP-1 mRNA levels and through AT2 receptors by unknown mechanisms.
Asunto(s)
Angiotensina II/farmacología , Proteínas de Ciclo Celular , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Miocardio/enzimología , Fosfoproteínas Fosfatasas , Animales , Calcio/fisiología , Células Cultivadas , Fosfatasa 1 de Especificidad Dual , Activación Enzimática/fisiología , Expresión Génica/efectos de los fármacos , Expresión Génica/fisiología , Proteínas Inmediatas-Precoces/genética , Miocardio/citología , Proteína Quinasa C/fisiología , Proteína Fosfatasa 1 , Proteína Tirosina Fosfatasa no Receptora Tipo 1 , Proteínas Tirosina Fosfatasas/genética , Ratas , Ratas Wistar , Receptor de Angiotensina Tipo 1 , Receptor de Angiotensina Tipo 2 , Receptores de Angiotensina/fisiología , Transcripción Genética/efectos de los fármacosRESUMEN
BACKGROUND: Although activation of the Ca(2+)-dependent phosphatase calcineurin has been reported to induce cardiomyocyte hypertrophy, whether calcineurin is involved in pressure overload-induced cardiac hypertrophy remains controversial. METHODS AND RESULTS: We examined in the present study the role of calcineurin in pressure overload-induced cardiac hypertrophy using transgenic mice that overexpress the dominant negative mutant of calcineurin specifically in the heart. There were no significant differences in body weight, blood pressure, heart rate, heart weight, and the cardiac calcineurin activity between the transgenic mice and their littermate wild-type mice at basal state. The activity of calcineurin was markedly increased by pressure overload produced by constriction of the abdominal aorta in the heart of wild-type mice but less increased in the heart of the transgenic mice. Pressure overload induced increases in heart weight, wall thickness of the left ventricle, and diameter of cardiomyocytes; reprogramming of expressions of immediate early response genes and fetal-type genes; activation of extracellular signal-regulated protein kinases; and fibrosis. All these hypertrophic responses were more prominent in the wild-type mice than in the transgenic mice. CONCLUSIONS: These results suggest that calcineurin plays a critical role in the development of pressure overload-induced cardiac hypertrophy.
Asunto(s)
Calcineurina/metabolismo , Cardiomegalia/etiología , Cardiomegalia/fisiopatología , Hipertensión/complicaciones , Hipertensión/fisiopatología , Animales , Aorta Abdominal/patología , Presión Sanguínea , Peso Corporal , Calcineurina/genética , Cardiomegalia/patología , Catálisis , Constricción Patológica , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Ecocardiografía , Activación Enzimática/genética , Fibrosis/patología , Expresión Génica , Genes Dominantes , Genes Inmediatos-Precoces , Ratones , Ratones Transgénicos , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Mutagénesis Sitio-Dirigida , Tamaño de los Órganos , Especificidad de Órganos/genéticaRESUMEN
BACKGROUND: Extracellular signal-regulated kinases (ERKs) and calcineurin have been reported to play important roles in the development of cardiac hypertrophy. We examined here the relation between calcineurin and ERKs in cardiomyocytes. METHODS AND RESULTS: Isoproterenol activated ERKs in cultured cardiomyocytes of neonatal rats, and the activation was abolished by chelation of extracellular Ca(2+) with EGTA, blockade of L-type Ca(2+) channels with nifedipine, or depletion of intracellular Ca(2+) stores with thapsigargin. Isoproterenol-induced activation of ERKs was also significantly suppressed by calcineurin inhibitors in cultured cardiomyocytes as well as in the hearts of mice. Isoproterenol failed to activate ERKs in either the cultured cardiomyocytes or the hearts of mice that overexpress the dominant negative mutant of calcineurin. Isoproterenol elevated intracellular Ca(2+) levels at both systolic and diastolic phases and dose-dependently activated calcineurin. Inhibition of calcineurin also attenuated isoproterenol-stimulated phosphorylation of Src, Shc, and Raf-1 kinase. The immunocytochemistry revealed that calcineurin was localized in the Z band, and isoproterenol induced translocation of calcineurin and ERKs into the nucleus. CONCLUSIONS: Calcineurin, which is activated by marked elevation of intracellular Ca(2+) levels by the Ca(2+)-induced Ca(2+) release mechanism, regulates isoproterenol-induced activation of ERKs in cardiomyocytes.
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Proteínas Adaptadoras Transductoras de Señales , Proteínas Adaptadoras del Transporte Vesicular , Calcineurina/metabolismo , Cardiomegalia/enzimología , Ventrículos Cardíacos/enzimología , Isoproterenol/farmacología , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Miocardio/enzimología , Agonistas Adrenérgicos beta/farmacología , Animales , Calcineurina/genética , Inhibidores de la Calcineurina , Calcio/antagonistas & inhibidores , Calcio/metabolismo , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio Tipo L/metabolismo , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina , Proteínas Quinasas Dependientes de Calcio-Calmodulina/biosíntesis , Proteínas Quinasas Dependientes de Calcio-Calmodulina/genética , Células Cultivadas , Quelantes/farmacología , Relación Dosis-Respuesta a Droga , Activación Enzimática/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Ventrículos Cardíacos/citología , Ventrículos Cardíacos/efectos de los fármacos , Ratones , Ratones Endogámicos ICR , Ratones Transgénicos , Proteína Quinasa 1 Activada por Mitógenos/genética , Mutagénesis Sitio-Dirigida , Miocardio/citología , Fosforilación/efectos de los fármacos , Proteínas/metabolismo , Proteínas Proto-Oncogénicas c-raf/metabolismo , Ratas , Ratas Wistar , Proteínas Adaptadoras de la Señalización Shc , Proteína Transformadora 1 que Contiene Dominios de Homología 2 de Src , Transfección , Familia-src Quinasas/metabolismoRESUMEN
The cardiac homeobox protein Nkx2-5 is essential in cardiac development, and mutations in Csx (which encodes Nkx2-5) cause various congenital heart diseases. Using the yeast two-hybrid system with Nkx2-5 as the 'bait', we isolated the T-box-containing transcription factor Tbx5; mutations in TBX5 cause heart and limb malformations in Holt-Oram syndrome (HOS). Co-transfection of Nkx2-5 and Tbx5 into COS-7 cells showed that they also associate with each other in mammalian cells. Glutathione S-transferase (GST) 'pull-down' assays indicated that the N-terminal domain and N-terminal part of the T-box of Tbx5 and the homeodomain of Nkx2-5 were necessary for their interaction. Tbx5 and Nkx2-5 directly bound to the promoter of the gene for cardiac-specific natriuretic peptide precursor type A (Nppa) in tandem, and both transcription factors showed synergistic activation. Deletion analysis showed that both the N-terminal domain and T-box of Tbx5 were important for this transactivation. A G80R mutation of Tbx5, which causes substantial cardiac defects with minor skeletal abnormalities in HOS, did not activate Nppa or show synergistic activation, whereas R237Q, which causes upper-limb malformations without cardiac abnormalities, activated the Nppa promoter to a similar extent to that of wildtype Tbx5. P19CL6 cell lines overexpressing wildtype Tbx5 started to beat earlier and expressed cardiac-specific genes more abundantly than did parental P19CL6 cells, whereas cell lines expressing the G80R mutant did not differentiate into beating cardiomyocytes. These results indicate that two different types of cardiac transcription factors synergistically induce cardiac development.
Asunto(s)
Proteínas de Homeodominio/metabolismo , Miocardio/citología , Péptido Natriurético Tipo-C/genética , Precursores de Proteínas/genética , Proteínas de Dominio T Box/metabolismo , Factores de Transcripción , Proteínas de Xenopus , Factor Natriurético Atrial , Sitios de Unión , Diferenciación Celular , Línea Celular , Genes Reporteros , Cardiopatías Congénitas/genética , Proteína Homeótica Nkx-2.5 , Humanos , Deformidades Congénitas de las Extremidades/genética , Mutación , Contracción Miocárdica/genética , Regiones Promotoras Genéticas , Unión Proteica , Síndrome , Activación TranscripcionalRESUMEN
Although several cardiac-specific transcription factors have been shown to play vital roles in various steps during the heart formation, the precise mechanism of the early stage of cardiogenesis has yet to be elucidated. By differential display technique, we tried to identify molecules that are expressed earlier than cardiac transcription factors such as CSX/NKX2-5 and GATA-4 and are involved in cardiomyocyte differentiation using the P19CL6 cell line, which efficiently differentiates into cardiomyocytes when treated with dimethyl sulfoxide. We isolated a novel gene designated Midori. Its deduced amino acid sequence contained an ATP/GTP-binding site, Ig-like domain, and Kringle-like domain. Northern blot analysis revealed that expression of Midori was restricted to the fetal and adult heart and adult skeletal muscle in mice. In whole mount in situ hybridization, Midori was expressed in cardiac crescent and developing heart but not in somites. The MIDORI protein was localized in the nucleus and overexpression of Midori induced expression of endogenous Midori itself, suggesting that MIDORI may act as a transcriptional regulator. Permanent P19CL6 cell lines overexpressing Midori more efficiently differentiated into cardiomyocytes than did parental cells, whereas those overexpressing the antisense Midori less efficiently differentiated. These results suggest that Midori may promote the differentiation of P19CL6 into cardiomyocytes.
Asunto(s)
Diferenciación Celular/genética , Proteínas Musculares/genética , Miocardio/citología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Linaje de la Célula , ADN Complementario , Embrión de Mamíferos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Ratones , Datos de Secuencia Molecular , Proteínas Musculares/química , Miocardio/metabolismoRESUMEN
We previously demonstrated that bone morphogenetic proteins (BMPs) induce cardiomyocyte differentiation through the mitogen-activated protein kinase kinase kinase TAK1. Transcription factors Smads mediate transforming growth factor-beta signaling and the ATF/CREB family transcription factor ATF-2 has recently been shown to act as a common target of the Smad and the TAK1 pathways. We here examined the role of Smads and ATF-2 in cardiomyocyte differentiation of P19CL6, a clonal derivative of murine P19 cells. Although P19CL6 efficiently differentiates into cardiomyocytes when treated with dimethyl sulfoxide, P19CL6noggin, a P19CL6 cell line constitutively overexpressing the BMP antagonist noggin, did not differentiate into cardiomyocytes. Cooverexpression of Smad1, a ligand-specific Smad, and Smad4, a common Smad, restored the ability of P19CL6noggin to differentiate into cardiomyocytes, whereas stable overexpression of Smad6, an inhibitory Smad, completely blocked differentiation of P19CL6, suggesting that the Smad pathway is necessary for cardiomyocyte differentiation. ATF-2 stimulated the betaMHC promoter activity by the synergistic manner with Smad1/4 and TAK1 and promoted terminal cardiomyocyte differentiation of P19CL6noggin, whereas overexpression of the dominant negative form of ATF-2 reduced the promoter activities of several cardiac-specific genes and inhibited differentiation of P19CL6. These results suggest that Smads, TAK1, and their common target ATF-2 cooperatively play a critical role in cardiomyocyte differentiation.
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Proteínas Adaptadoras Transductoras de Señales , Proteínas Portadoras/metabolismo , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Proteínas de Unión al ADN/metabolismo , Péptidos y Proteínas de Señalización Intracelular , Fibras Musculares Esqueléticas/citología , Miocardio/citología , Transactivadores/metabolismo , Factores de Transcripción/metabolismo , Factor de Transcripción Activador 2 , Animales , Proteínas Morfogenéticas Óseas/metabolismo , Diferenciación Celular/fisiología , Línea Celular , Proteínas de Unión al ADN/genética , Expresión Génica/fisiología , Fibras Musculares Esqueléticas/enzimología , Proteínas/genética , Proteínas Smad , Proteína smad6 , Transactivadores/genéticaRESUMEN
gp130, a common receptor for the interleukin 6 family, plays pivotal roles in growth and survival of cardiac myocytes. In the present study, we examined the role of gp130 in pressure overload-induced cardiac hypertrophy using transgenic (TG) mice, which express a dominant negative mutant of gp130 in the heart under the control of alpha myosin heavy chain promoter. TG mice were apparently healthy and fertile. There were no differences in body weight and heart weight between TG mice and littermate wild type (WT) mice. Pressure overload-induced increases in the heart weight/body weight ratio, ventricular wall thickness, and cross-sectional areas of cardiac myocytes were significantly smaller in TG mice than in WT mice. Northern blot analysis revealed that pressure overload-induced up-regulation of brain natriuretic factor gene and down-regulation of sarcoplasmic reticulum Ca(2+) ATPase 2 gene were attenuated in TG mice. Pressure overload activated ERKs and STAT3 in the heart of WT mice, whereas pressure overload-induced activation of STAT3, but not of ERKs, was suppressed in TG mice. These results suggest that gp130 plays a critical role in pressure overload-induced cardiac hypertrophy possibly through the STAT3 pathway.
Asunto(s)
Antígenos CD/fisiología , Cardiomegalia/fisiopatología , Glicoproteínas de Membrana/fisiología , Animales , Antígenos CD/genética , Peso Corporal , Receptor gp130 de Citocinas , Proteínas de Unión al ADN/metabolismo , Activación Enzimática , Regulación de la Expresión Génica , Etiquetado Corte-Fin in Situ , Masculino , Glicoproteínas de Membrana/genética , Ratones , Ratones Transgénicos , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Tamaño de los Órganos , Presión , Factor de Transcripción STAT3 , Transactivadores/metabolismoRESUMEN
BACKGROUND: It remains unclear how hemodynamic overload induces cardiac hypertrophy. Recently, activation of calcium-dependent phosphatase, calcineurin, has been elucidated to induce cardiac hypertrophy. In the present study, we examined the role of calcineurin in load-induced cardiac hypertrophy by using Dahl salt-sensitive (DS) rats, which develop both pressure and volume overload when fed a high salt diet. METHODS AND RESULTS: In the DS rat heart, the activity of calcineurin was increased and cardiac hypertrophy was induced by high salt diet. Treatment of DS rats with the calcineurin inhibitor FK506 (0.1 or 0.01 mg/kg twice daily) from the age of 6 weeks to 12 weeks inhibited the activation of calcineurin in the heart in a dose-dependent manner and attenuated the development of load-induced cardiac hypertrophy and fibrosis without change of hemodynamic parameters. Additionally, treatment with 0.1 mg/kg twice daily but not with 0.01 mg/kg twice daily of FK506 from the age of 12 weeks to 16 weeks induced regression of cardiac hypertrophy in DS rats. Load-induced reprogramming of gene expression was also suppressed by the FK506 treatment. CONCLUSIONS: These results suggest that calcineurin is involved in the development of cardiac hypertrophy in rats with salt-sensitive hypertension and that inhibition of calcineurin could induce regression of cardiac hypertrophy.
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Inhibidores de la Calcineurina , Cardiomegalia/tratamiento farmacológico , Hipertensión/complicaciones , Tacrolimus/farmacología , Animales , Presión Sanguínea/efectos de los fármacos , Calcineurina/metabolismo , Cardiomegalia/complicaciones , Cardiomegalia/metabolismo , Cardiomegalia/patología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Esquema de Medicación , Electrocardiografía , Fibrosis Endomiocárdica/patología , Regulación de la Expresión Génica/efectos de los fármacos , Corazón/efectos de los fármacos , Hipertensión/inducido químicamente , Inyecciones Intramusculares , Masculino , Miocardio/metabolismo , Miocardio/patología , Ratas , Ratas Endogámicas Dahl , Inducción de Remisión , Cloruro de Sodio Dietético , Tacrolimus/administración & dosificaciónRESUMEN
Apoptosis of cardiac myocytes is one of the causes of heart failure. Here we examine the mechanism by which the activation of beta-adrenergic receptor induces cardiomyocyte apoptosis. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling and DNA ladder analyses revealed that isoproterenol (Iso) induced the apoptosis of cardiac myocytes of neonatal rats through an increase in intracellular Ca(2+) levels. The Iso-induced cardiomyocyte apoptosis was strongly inhibited by the L-type Ca(2+) channel antagonist nifedipine and by the calcineurin inhibitors cyclosporin A and FK506. Iso reduced the phosphorylation levels of the proapoptotic Bcl-2 family protein Bad and induced cytochrome c release from mitochondria to the cytosol through calcineurin activation. Infusion of Iso increased calcineurin activity by approximately 3-fold in the hearts of wild-type mice but not in the hearts of transgenic mice that overexpress dominant negative mutants of calcineurin. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling analysis revealed that infusion of Iso induced apoptosis of cardiac myocytes and that the number of apoptotic cardiomyocytes was significantly less in the hearts of the transgenic mice compared with the wild-type mice. These results suggest that calcineurin plays a critical role in Iso-induced apoptosis of cardiac myocytes, possibly through dephosphorylating Bad.
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Apoptosis/fisiología , Calcineurina/fisiología , Miocardio/metabolismo , Receptores Adrenérgicos beta/fisiología , Animales , Calcineurina/genética , Calcio/metabolismo , Grupo Citocromo c/metabolismo , Citosol/efectos de los fármacos , Citosol/metabolismo , Etiquetado Corte-Fin in Situ , Isoproterenol/farmacología , Ratones , Ratones Transgénicos , Mitocondrias Cardíacas/efectos de los fármacos , Mitocondrias Cardíacas/metabolismo , Miocardio/citología , Fosforilación , Ratas , Ratas WistarRESUMEN
A homeodomain-containing transcription factor Csx/Nkx-2.5 is an important regulator of cardiogenesis in mammals. Three different mutants, Gln170ter (designated A) and Thr178Met (designated B) in the helix 2 of the homeodomain and Gln198ter mutation (designated C) just after homeodomain, have been reported to cause atrial septal defect with atrial ventricular block. We here examined the functions of these three mutants of Csx/Nkx-2.5. The atrial natriuretic peptide (ANP) promoter was activated by wild type Csx/Nkx-2.5 (WT, approximately 8-fold), B ( approximately 2-fold), and C ( approximately 6-fold) but not by A. When A, B, or C was cotransfected into COS-7 cells with the same amount of WT, WT-induced activation of the ANP promoter was attenuated by A and B (A > B), whereas C further enhanced the activation. Immunocytochemical analysis using anti-Myc tag antibody indicated that transfected Myc-tagged WT, B, and C were localized in the nucleus of both COS-7 cells and cardiomyocytes of neonatal rats, whereas A was distributed diffusely in the cytoplasm and nucleus in COS-7 cells. Electrophoretic mobility shift assay showed that Csx/Nkx-2.5-binding sequences were bound strongly by WT and C, weakly by B, but not by A. Immunoprecipitation and GST pull-down assay revealed that WT and all mutants interacted with GATA-4. The synergistic activation of the ANP promoter by WT and GATA-4 was further enhanced by C but was inhibited by A and B. In the cultured cardiomyocytes, overexpression of C but not WT, A, or B, induced apoptosis. These results suggest that although the three mutants induce the same cardiac phenotype, transactivation ability and DNA binding ability are different among the three mutants and that apoptosis may be a cause for C-induced cardiac defect.
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Factor Natriurético Atrial/genética , Cardiopatías/congénito , Cardiopatías/etiología , Proteínas de Homeodominio/genética , Mutación , Factores de Transcripción/genética , Transcripción Genética , Proteínas de Xenopus , Animales , Animales Recién Nacidos , Apoptosis , Células COS , Núcleo Celular/metabolismo , Células Cultivadas , Citoplasma/metabolismo , Proteínas de Unión al ADN/metabolismo , Electroforesis en Gel de Poliacrilamida , Factor de Transcripción GATA4 , Regulación de la Expresión Génica , Genes Reporteros , Glutatión Transferasa/metabolismo , Defectos del Tabique Interatrial/genética , Proteína Homeótica Nkx-2.5 , Humanos , Inmunohistoquímica , Etiquetado Corte-Fin in Situ , Microscopía Fluorescente , Miocardio/metabolismo , Miocardio/patología , Proteínas Nucleares/metabolismo , Fenotipo , Plásmidos/metabolismo , Pruebas de Precipitina , Regiones Promotoras Genéticas , Unión Proteica , Ratas , Receptores Purinérgicos P1/metabolismo , Factor de Respuesta Sérica , Factores de Transcripción/metabolismo , Activación Transcripcional , TransfecciónRESUMEN
Glycosyl phosphatidylinositols (GPIs) are used to anchor many proteins to the cell surface membrane and are utilized in all eukaryotic cells. GPI anchoring units are attached to proteins via a transamidase reaction mediated by a GPI transamidase complex. We isolated one of the components of this complex, mGPAA1 (murine GPI anchor attachment), by the signal sequence trap method. mGPAA1 cDNA is about 2 kb in length and encodes a putative 621 amino acid protein. The mGPAA1 gene has 12 small exons and 11 small introns. mGPAA1 mRNA is ubiquitously expressed in mammalian cells, and in situ hybridization analysis revealed that it is abundant in the choroid plexus, skeletal muscle, osteoblasts of rib, and occipital bone in mouse embryos. Its expression levels and transamidation efficiency decreased with differentiation of embryonic stem cells. The 3T3 cell lines expressing antisense mGPAA1 failed to express GPI-anchored proteins on the cell surface membrane.
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Clonación Molecular , Proteínas/genética , Células 3T3/metabolismo , Células 3T3/fisiología , Fosfatasa Alcalina/metabolismo , Secuencia de Aminoácidos/genética , Animales , Elementos sin Sentido (Genética)/metabolismo , Secuencia de Bases/genética , Antígenos CD55/metabolismo , ADN Complementario/genética , Embrión de Mamíferos/metabolismo , Genoma , Glicoproteínas de Membrana , Ratones , Datos de Secuencia Molecular , Proteínas Gestacionales/metabolismo , ARN Mensajero/metabolismo , Células Madre/metabolismo , Distribución TisularRESUMEN
Cardiac homeobox gene Csx/Nkx-2.5 is essential for normal heart development and morphogenesis and is the earliest marker for cardiogenesis. To elucidate the regulatory mechanisms of Csx/Nkx-2.5 expression, we have isolated and characterized the upstream regulatory region of human Csx/Nkx-2.5 (CSX1). Transfection of the reporter gene containing a 965-bp CSX1 5' flanking region indicated that this region confers cardiomyocyte-predominant expression of CSX1. Deletion and mutational analyses revealed two positive cis-regulatory elements in this region that are essential for CSX1 expression in cardiomyocytes. Electrophoretic mobility shift assay revealed that nuclear proteins prepared from cardiac myocytes bound to these elements in a sequence-specific manner. The identification of cis-regulatory sequences of the Csx/Nkx-2.5 gene will facilitate further analysis for the upstream regulatory factors that control the expression of Csx/Nkx-2.5 and the process of vertebrate heart development.
Asunto(s)
Regulación de la Expresión Génica/genética , Genes Homeobox/genética , Proteínas de Homeodominio/genética , Miocardio/metabolismo , Regiones Promotoras Genéticas/genética , Factores de Transcripción/genética , Transcripción Genética/genética , Animales , Secuencia de Bases , Sitios de Unión , Células COS , Clonación Molecular , ADN/genética , ADN/metabolismo , Proteínas de Unión al ADN/metabolismo , Proteína Homeótica Nkx-2.5 , Humanos , Datos de Secuencia Molecular , Mutación/genética , Miocardio/citología , Proteínas Nucleares/metabolismo , Especificidad de Órganos , Elementos de Respuesta/genética , TransfecciónRESUMEN
A cardiac homeobox-containing gene Csx/Nkx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. Targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. To elucidate the role of Csx/Nkx2-5 in the adult heart, we generated transgenic mice which overexpress human Csx/Nkx2-5. The transgene was expressed abundantly in the heart and the skeletal muscle. mRNA levels of several cardiac genes including natriuretic peptides, CARP, MLC2v, and endogenous Csx/Nkx2-5 were increased in the ventricle of the transgenic mice. Electron microscopic analysis revealed that the ventricular myocardium of the transgenic mice had many secretory granules, which disappeared after administration of vasopressin. These results suggest that Csx/Nkx2-5 regulates many cardiac genes and induces formation of secretory granules in the adult ventricle.
Asunto(s)
Factor Natriurético Atrial/genética , Miosinas Cardíacas , Regulación de la Expresión Génica , Proteínas de Homeodominio/metabolismo , Miocardio/metabolismo , Factores de Transcripción/metabolismo , Proteínas de Xenopus , Animales , Corazón/embriología , Ventrículos Cardíacos , Proteína Homeótica Nkx-2.5 , Proteínas de Homeodominio/genética , Humanos , Ratones , Ratones Transgénicos , Proteínas Musculares/genética , Músculo Esquelético/metabolismo , Cadenas Ligeras de Miosina/genética , Péptido Natriurético Encefálico/genética , Proteínas Nucleares/genética , Isoformas de Proteínas/genética , Proteínas Represoras/genética , Factores de Transcripción/genéticaRESUMEN
Although smooth muscle cells (SMCs) are critical components of the circulatory system, the regulatory mechanisms of SMC differentiation remain largely unknown. In the present study, we examined the mechanism of SMC differentiation by using Xenopus laevis SM22alpha (XSM22alpha) as an SMC-specific marker. XSM22alpha cDNA contained a 600-bp open reading frame, and the predicted amino acid sequences were highly conserved in evolution. XSM22alpha transcripts were first detected in heart anlage, head mesenchyme, and the dorsal side of the lateral plate mesoderm at the tail-bud stage, possibly representing the precursors of muscle lineage. At the tadpole stage, XSM22alpha transcripts were restricted to the vascular and visceral SMCs. XSM22alpha was strongly induced by basic fibroblast growth factor (FGF) in animal caps. Although expressions of Xenopus cardiac actin were not affected by the expression of a dominant-negative FGF receptor, its injection dramatically suppressed the XSM22alpha expression. These results suggest that XSM22alpha is a useful molecular marker for the SMC lineage in Xenopus and that FGF signaling plays an important role in the induction of XSM22alpha and in the differentiation of SMCs.
Asunto(s)
Factor 2 de Crecimiento de Fibroblastos/fisiología , Regulación del Desarrollo de la Expresión Génica , Proteínas de Microfilamentos , Proteínas Musculares/genética , Xenopus laevis/embriología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario/química , Factor 2 de Crecimiento de Fibroblastos/farmacología , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Datos de Secuencia Molecular , Proteínas Musculares/análisis , Proteínas Musculares/química , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Distribución Tisular , Xenopus laevis/crecimiento & desarrolloRESUMEN
Bone morphogenetic proteins (BMPs) have been shown to induce ectopic expression of cardiac transcription factors and beating cardiomyocytes in nonprecardiac mesodermal cells in chicks, suggesting that BMPs are inductive signaling molecules that participate in the development of the heart. However, the precise molecular mechanisms by which BMPs regulate cardiac development are largely unknown. In the present study, we examined the molecular mechanisms by which BMPs induce cardiac differentiation by using the P19CL6 in vitro cardiomyocyte differentiation system, a clonal derivative of P19 embryonic teratocarcinoma cells. We established a permanent P19CL6 cell line, P19CL6noggin, which constitutively overexpresses the BMP antagonist noggin. Although almost all parental P19CL6 cells differentiate into beating cardiomyocytes when treated with 1% dimethyl sulfoxide, P19CL6noggin cells did not differentiate into beating cardiomyocytes nor did they express cardiac transcription factors or contractile protein genes. The failure of differentiation was rescued by overexpression of BMP-2 or addition of BMP protein to the culture media, indicating that BMPs were indispensable for cardiomyocyte differentiation in this system. Overexpression of TAK1, a member of the mitogen-activated protein kinase kinase kinase superfamily which transduces BMP signaling, restored the ability of P19CL6noggin cells to differentiate into cardiomyocytes and concomitantly express cardiac genes, whereas overexpression of the dominant negative form of TAK1 in parental P19CL6 cells inhibited cardiomyocyte differentiation. Overexpression of both cardiac transcription factors Csx/Nkx-2.5 and GATA-4 but not of Csx/Nkx-2.5 or GATA-4 alone also induced differentiation of P19CL6noggin cells into cardiomyocytes. These results suggest that TAK1, Csx/Nkx-2.5, and GATA-4 play a pivotal role in the cardiogenic BMP signaling pathway.
Asunto(s)
Proteínas Morfogenéticas Óseas/farmacología , Quinasas Quinasa Quinasa PAM/metabolismo , Miocardio/citología , Factores de Transcripción/metabolismo , Proteínas de Xenopus , Proteínas Morfogenéticas Óseas/antagonistas & inhibidores , Proteínas Portadoras , Diferenciación Celular , Proteínas de Unión al ADN/metabolismo , Factor de Transcripción GATA4 , Proteína Homeótica Nkx-2.5 , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Quinasas Quinasa Quinasa PAM/genética , Modelos Biológicos , Biosíntesis de Proteínas , Proteínas/genética , Proteínas Recombinantes/metabolismo , Factores de Transcripción/genética , Células Tumorales CultivadasRESUMEN
Although the cardiac homeobox gene Csx/Nkx-2.5 is essential for normal heart development, little is known about its regulatory mechanisms. In a search for the downstream target genes of Csx/Nkx-2. 5, we found that the atrial natriuretic peptide (ANP) gene promoter was strongly transactivated by Csx/Nkx-2.5. Deletion and mutational analyses of the ANP promoter revealed that the Csx/Nkx-2.5-binding element (NKE2) located at -240 was required for high level transactivation by Csx/Nkx-2.5. We also found that Csx/Nkx-2.5 and GATA-4 displayed synergistic transcriptional activation of the ANP promoter, and in contrast to previous reports (Durocher, D., Charron, F., Warren, R., Schwartz, R. J., and Nemer, M. (1997) EMBO J. 16, 5687-5696; Lee, Y., Shioi, T., Kasahara, H., Jobe, S. M., Wiese, R. J., Markham, B., and Izumo, S (1998) Mol. Cell. Biol. 18, 3120-3129), this synergism was dependent on binding of Csx/Nkx-2.5 to NKE2, but not on GATA-4-DNA interactions. Although GATA-4 also potentiated the Csx/Nkx-2.5-induced transactivation of the artificial promoter that contains multimerized Csx/Nkx-2.5-binding sites, Csx/Nkx-2.5 reduced the GATA-4-induced transactivation of the GATA-4-dependent promoters. These findings indicate that the cooperative transcriptional regulation mediated by Csx/Nkx-2.5 and GATA-4 is promoter context-dependent and suggest that the complex cis-trans interactions may fine-tune gene expression in cardiac myocytes.
Asunto(s)
Proteínas de Unión al ADN/metabolismo , Corazón/crecimiento & desarrollo , Proteínas de Homeodominio/metabolismo , Factores de Transcripción/metabolismo , Transcripción Genética , Animales , Sitios de Unión/genética , ADN/química , ADN/metabolismo , Sinergismo Farmacológico , Factor de Transcripción GATA4 , Regulación del Desarrollo de la Expresión Génica , Proteína Homeótica Nkx-2.5 , Humanos , Ratones , Miocardio/metabolismo , Regiones Promotoras Genéticas , Ratas , Análisis de Secuencia de ADN , Activación TranscripcionalRESUMEN
Atrial septal defect (ASD) is the most common form of congenital cardiac defect in humans. Recently, point mutations in the cardiac homeobox gene CSX/NKX2-5 have been reported to cause the autosomal dominant form of familial ASD. Notably, all the affected patients exhibit atrioventricular conduction disturbance and some of them died suddenly. The first case of familial ASD with a mutation of the CSX/NKX2-5 gene in a Japanese patient is reported here. Identification of CSX/NKX2-5 mutations in ASD patients would be very important because the existence of such mutations may predict sudden cardiac death.
Asunto(s)
Bloqueo Cardíaco/genética , Defectos del Tabique Interatrial/genética , Proteínas de Homeodominio/genética , Factores de Transcripción/genética , Bloqueo Cardíaco/fisiopatología , Defectos del Tabique Interatrial/fisiopatología , Proteína Homeótica Nkx-2.5 , Humanos , Masculino , Persona de Mediana Edad , Mutación PuntualRESUMEN
The effect on pregnant rats of individual amino acids added excessively to the diet or intermittently administered in excess directly with a stomach tube was examined. When methionine was excessively added to the diet at a 5% level, amino acid imbalance in plasma was induced and food intake decreased remarkably to approximately one-fifth of that of control rats. However, when administered directly into the stomach, food intake remained almost normal, except for excess of methionine, tryptophan or threonine, and an adverse effect of excess amino acid was not observed. However, in the case of a decrease in food intake, various adverse effects were observed. This was especially so for the group that was administered methionine and whose food intake was far below that of the control group: decrease in maternal body weight, delayed growth of products of conception, and further, significant decrease in brain and liver cells of the fetus were observed. In addition, the changes in nitrogen balance were well correlated with the changes in bodyweight in all groups. In the methionine group, in particular, nitrogen balance was negative throughout the period because of a decrease in food intake, and utilization of dietary nitrogen was inferior. The majority of the various lesions observed with excess administration of individual amino acids were not due to a direct effect of excess amino acid but were mainly caused by the remarkable decrease in food intake.