RESUMEN
Motor neuron (MN) mitochondrial abnormalities and elevation in spinal fluid levels of the inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) have been implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS). The mechanism of neuron death in ALS remains unclear, along with the contributions of mitochondrial dysfunction and inflammation in the process. Cell cultures enriched for MN derived from embryonic rat spinal cords were established and directly exposed in vitro to recombinant TNF-alpha for varying lengths of time. Although cytokine exposure for up to 4 days failed to induce MN death, mitochondrial changes were observed shortly after initiating treatment. Our results demonstrate that TNF-alpha induced mitochondrial redistribution toward the soma in MN. We postulate that inflammation may precede, and in fact cause, the mitochondrial changes observed in ALS tissue.
Asunto(s)
Mitocondrias/efectos de los fármacos , Neuronas Motoras/efectos de los fármacos , Factor de Necrosis Tumoral alfa/farmacología , Animales , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Técnicas In Vitro , Microscopía por Video , Mitocondrias/ultraestructura , Neuronas Motoras/ultraestructura , Necrosis , Ratas , Ratas Sprague-Dawley , Fijación del TejidoRESUMEN
MDGC-ECD procedures have been used to provide insight into the compositional complexity of some of the specific peaks or clusters observed in the gas chromatographic analysis of a technical toxaphene standard, with reference to individual toxaphene congeners (Parlar # components) that are flow commercially available. These investigations have focussed initially upon those peaks and clusters recently identified (Shoeib. M., Brice, K.A., Hoff, R., 1999. Chemosphere 39, 849-871) as dominant constituents of background ambient air. Multiple electron-capturing components have been found to be present in all the species studied: the available individual toxaphene congeners have been matched against these components where possible. In similar fashion, the responses obtained in equivalent gas chromatographic elution windows from the analysis of typical processed air sample extracts have been investigated, with the results showing clear differences relative to the patterns found in the technical toxaphene standard. In most cases, the air sample shows reduced complexity with fewer components present in the cluster. Also, the presence of interfering responses (due to PCBs and other organochlorines) is quite apparent and significant, showing that major problems and errors could arise when using single-column GC-ECD procedures for quantitation of toxaphene in environmental samples. The presence of certain of the Parlar species in the air samples has been confirmed and in most cases these represent the dominant toxaphene component found in the targeted cluster. Furthermore, the persistence of certain congeners in the atmospheric samples appears to be strongly dependent upon chemical structure, since the congeners in question possess an alternating exo-endo chlorine substitution pattern around the six-membered ring in the bornane skeleton. Such persistence is probably the result of lower metabolization of toxaphene residues in soils, water and sediments leading to a similar pattern in the atmosphere following volatilization.
Asunto(s)
Contaminantes Atmosféricos/análisis , Cromatografía de Gases/métodos , Insecticidas/análisis , Toxafeno/análisis , Insecticidas/química , Solventes , Toxafeno/químicaRESUMEN
A reliable analytical method has been developed using GC-ECNIMS for the determination of individual toxaphene congeners in ambient air. To allow a reasonable comparison with previous data for toxaphene reported by Muir and co-workers using GC-ECD, this method has adopted their approach of focussing upon the identification and quantification of specific peaks or clusters ("T" species) typically observed in environmental samples, with the sum of these "T" species then being reported as "total toxaphene". Technical toxaphene has been used as the analytical standard, but independent response factors have been assigned to the target peaks and clusters. Because of the appreciable variability in ECNIMS response shown by individual toxaphene congeners, this is considered to be a reasonable and potentially more accurate procedure than the application of a "single response factor" used by many other workers. The methodology has been used for the determination of toxaphene in air samples collected over the annual cycle in 1992 and then from October 1995 to September 1997 at Point Petre, Ontario. Of the forty-four calibrated components, only 10 were detected in all of the air samples collected over the latter 2-year period. Airborne concentrations of toxaphene (defined as the sum of the calibrated components) range from 0.9 pg/m3 to 10.1 pg/m3. A clear seasonality has been observed, with a summer-to-winter concentration ratio of about 6.
Asunto(s)
Contaminantes Atmosféricos/análisis , Cromatografía de Gases y Espectrometría de Masas/métodos , Insecticidas/análisis , Toxafeno/análisis , OntarioRESUMEN
This paper compares three analytical methods that are often used to analyze composition of atmospheric aerosol: Ion Chromatography (IC), Proton Induced X-ray Emission (PIXE), and X-ray Fluorescence (XRF). Three monitoring studies are discussed: (1) a comparison of air particulate data collected by several independent sampler/analytical technique suites run by different laboratories; (2) a study involving two identical samplers and a single suite of analytical techniques; and (3) analysis of identical aerosol samples by two different techniques (XRF vs. PIXE). While the XRF versus PIXE project shows a very good agreement for most elements, the first interlaboratory study demonstrates the "real-life" noise introduced into the final data set by various sampling complications and different collection characteristics of the samplers used. The XRF versus PIXE study also revealed an unexplained deviation in measured sulphur concentrations for very lightly loaded samples. In the five-sampler comparison, two data sets provided by IC were approximately 20% lower than the three data sets obtained by PIXE and XRF. When two identical IMPROVE-compatible samplers were used and samples were subjected to similar procedures and the same analytical techniques, the variability between the two air concentration data sets significantly decreased.
Asunto(s)
Aerosoles/análisis , Contaminación del Aire/análisis , Monitoreo del Ambiente/métodos , Cromatografía , Sensibilidad y Especificidad , Espectrometría por Rayos XAsunto(s)
Enfermedades de los Gatos/epidemiología , Toxoplasmosis Animal/epidemiología , Animales , Animales Salvajes , Anticuerpos/análisis , Australia , Gatos , Femenino , Pruebas de Inhibición de Hemaglutinación/veterinaria , Macropodidae/inmunología , Masculino , Muridae/inmunología , Conejos/inmunología , Toxoplasma/inmunología , Toxoplasma/aislamiento & purificaciónRESUMEN
During the steady-state period of activity of La Soufriere Volcano in 1979, the mass emissions of sulfur dioxide into the troposphere amounted to a mean value of 339 +/- 126 metric tons per day. This value is similar to the sulfur dioxide emissions of other Central American volcanoes but less than those measured at Mount Etna, an exceptionally strong volcanic source of sulfur dioxide.
RESUMEN
A procedure has been established to obtain the design parameters of the masks used in dispersive correlation spectrometers. The SNR equations are used to derive the mask equations. These are later solved for some simplified band models, and the obtained parameters are used as the initial values in an iterative program which determines the final design parameters. The calculations can be performed for various backgrounds and instrumental line profiles in order to achieve the best compromise for the detection of pollutants, and other trace gases, in the atmosphere.