RESUMEN
OBJECTIVE: Parents of children with cancer are at risk for developing psychosocial problems. The present study aims to evaluate the effect of an online group intervention (Op Koers Online, in English: On Track Online) on psychosocial wellbeing and coping skills. METHODS: Parents of a child with cancer (diagnosis <5 years ago) participated in a randomized controlled trial. In six consecutive (and one booster-) protocolled sessions in an online chatroom, trained psychologists and social workers taught coping skills using cognitive behavioral and acceptance and commitment techniques. Questionnaires assessed anxiety, depression, distress, situation-specific emotional reactions and coping skills (Op Koers Questionnaire/Cognitive Coping Strategies Scale Parent Form) and evaluated the intervention. Linear mixed-model analyses were performed to detect differences between the conditions in changes over time; T0-T1 and T0-T2 (6-week and 6-month follow-up), and to detect changes in scores T2-T3 (12-month follow-up) for the intervention group only. RESULTS: 89 parents were included in analyses (mean age 41.9 years, 86% female, 62%/38% post/during treatment of their child). Beneficial intervention effects (p < 0.05) were found at T1 for anxiety, depression, distress, loneliness and relaxation, and at T2 for anxiety, uncertainty and relaxation. In the intervention condition, scores did not change from T2 to T3, except loneliness that decreased and relaxation that improved. All effect sizes were small to medium (ß = -0.21 to 0.46). Parents were generally positive about the intervention. CONCLUSIONS: Op Koers Online for parents of children with cancer has a positive effect on psychosocial wellbeing and the coping skill relaxation. Implementation is recommended to prevent psychosocial problems. CLINICAL TRIAL REGISTRATION: Dutch Trial Register https://onderzoekmetmensen.nl/en NL73763.041.20.
Asunto(s)
Neoplasias , Padres , Niño , Humanos , Femenino , Adulto , Masculino , Padres/psicología , Estrés Psicológico/terapia , Estrés Psicológico/psicología , Ansiedad/terapia , Habilidades de Afrontamiento , Neoplasias/terapia , Neoplasias/psicologíaRESUMEN
We have investigated the primary charge separation processes in Rb. capsulatus reaction centers (RCs) bearing the mutations Phe(L181) --> Tyr, Tyr(M208) --> Phe, and Leu(M212) --> His. In the YFH mutant, decay of the excited primary electron donor P occurs with an 11 +/- 2 ps time constant and is trifurcated to give (1) internal conversion to the ground state ( approximately 10% yield), (2) charge separation to the L side of the RC ( approximately 60% yield), and (3) electron transfer to the M-side bacteriopheophytin BPh(M) ( approximately 30% yield). These results relate previous work in which the ionizable residues Lys (at L178) and Asp (at M201) have been used to facilitate charge separation to the M side of the RC, and the widely studied L181 and M208 mutants. One conclusion that comes from this work is that the Tyr (M208) --> Phe and Gly(M201) --> Asp mutations near the L-side bacteriochlorophyll (BChl(L)) raise the free energy of P(+)BChl(L)(-) by comparable amounts. The results also suggest that the free energy of P(+)BChl(M)(-) is lowered more substantially by a Tyr at L181 than a Lys at L178. The results on the YFH mutant further demonstrate that the free energy differences between the L- and M-side charge-separated states play a significant role in the directionality of charge separation in the wild-type RC, and place limits on the contributing role of differential electronic matrix elements on the two sides of the RC.
Asunto(s)
Proteínas del Complejo del Centro de Reacción Fotosintética/química , Transporte de Electrón , Cinética , Complejos de Proteína Captadores de Luz , Mutación , Fenilalanina/química , Fotoquímica , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Rhodobacter capsulatus/química , Tirosina/químicaAsunto(s)
Reactivos de Enlaces Cruzados , Sondas de ADN , Pruebas Genéticas/métodos , Antígenos HLA/genética , Hemocromatosis/genética , Antígenos de Histocompatibilidad Clase I/genética , Proteínas de la Membrana , Donantes de Sangre , Genotipo , Proteína de la Hemocromatosis , Humanos , Mutación , Hibridación de Ácido Nucleico , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
We have prepared a linear array of chromophores consisting of a perylene input unit, a bis(free base porphyrin) transmission unit, and a free base phthalocyanine output unit for studies in artificial photosynthesis and molecular photonics. The synthesis involved four stages: (1) a rational synthesis of trans-AB2C-porphyrin building blocks each bearing one meso-unsubstituted position, (2) oxidative, meso,meso coupling of the zinc porphyrin monomers to afford a bis(zinc porphyrin) bearing one phthalonitrile group and one iodophenyl group, (3) preparation of a bis(porphyrin)-phthalocyanine array via a mixed cyclization involving the bis(free base porphyrin) and 4-tert-butylphthalonitrile, and (4) Pd-mediated coupling of an ethynylperylene to afford a perylene-bis(porphyrin)-phthalocyanine linear array. The perylene-bis(porphyrin)-phthalocyanine array absorbs strongly across the visible spectrum. Excitation at 490 nm, where the perylene absorbs preferentially, results in fluorescence almost exclusively from the phthalocyanine (phi(f) = 0.78). The excited phthalocyanine forms with time constants of 2 ps (90%) and 13 ps (10%). The observed time constants resemble those of corresponding phenylethyne-linked dyads, including a perylene-porphyrin (< or = 0.5 ps) and a porphyrin-phthalocyanine (1.1 ps (70%) and 8 ps (30%)). The perylene-bis(porphyrin)-phthalocyanine architecture exhibits efficient light-harvesting properties and rapid funneling of energy in a cascade from perylene to bis(porphyrin) to phthalocyanine.
RESUMEN
Qy-excitation resonance Raman (RR) spectra are reported for two mutant reaction centers (RCs) from Rhodobacter capsulatus in which the photoactive bacteriopheophytin (BPhL) is replaced by a bacteriochlorophyll (BChl) molecule, designated beta. The pigment change in both mutants is induced via introduction of a histidine residue near the photoactive cofactor. In one mutant, L(M212)H, the histidine is positioned over the core of the cofactor and serves as an axial ligand to the Mg+2 ion. In the other mutant, F(L121)H/F(L97)V, the histidine is positioned over ring V of the cofactor, which is nominally too distant to permit bonding to the Mg+2 ion. The salient observations are as follows: (1) The beta cofactor in F(L121)H/F(L97)V RCs is a five-coordinate BChl molecule. However, there is no evidence for the formation of a Mg-His bond. This bond is either much weaker than in the L(M212)H RCs or completely absent, the latter implying coordination by an alternative ligand. The different axial ligation for beta in the F(L121)H/F(L97)V versus L(M212)H RCs in turn leads to different conformations of the BChl macrocycles. (2) The C9-keto group of beta in F(L121)H/F(L97)V RCs is free of hydrogen bonding interactions, unlike the L(M212)H RCs in which the C9-keto of beta is hydrogen bonded to Glu L104. The interactions between other peripheral substituents of beta and the protein are also different in the F(L121)H/F(L97)V RCs versus L(M212)H RCs. Accordingly, the position and orientation of beta in the protein is different in the two beta-containing RCs. Nonetheless, previous studies have shown that the primary electron transfer reactions are very similar in the two mutants but differ in significant respects compared to wild-type RCs. Collectively, these observations indicate that changes in the conformation of a photoactive tetrapyrrole macrocycle or its interactions with the protein do not necessarily lead to significantly perturbed photochemistry and do not underlie the altered primary events in beta-type RCs.
Asunto(s)
Bacterioclorofilas/química , Feofitinas/química , Proteínas del Complejo del Centro de Reacción Fotosintética/química , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Bacterioclorofilas/genética , Bacterioclorofilas/metabolismo , Complejos de Proteína Captadores de Luz , Estructura Molecular , Feofitinas/genética , Feofitinas/metabolismo , Fotoquímica , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Conformación Proteica , Rhodobacter capsulatus/química , Rhodobacter capsulatus/genética , Espectrometría Raman/métodos , Relación Estructura-Actividad , VibraciónRESUMEN
We report the primary charge separation events in a series of Rhodobacter capsulatus reaction centers (RCs) that have been genetically modified to contain a lysine near the bacteriochlorophyll molecule, BChl(M), on the nonphotoactive M-side of the RC. Using wild type and previously constructed mutants as templates, we substituted Lys for the native Ser residue at position 178 on the L polypeptide to make the S(L178)K single mutant, the S(L178)K/G(M201)D and S(L178)K/L(M212)H double mutants, and the S(L178)K/G(M201)D/L(M212)H triple mutant. In the triple mutant, the decay of the photoexcited primary electron donor (P) occurs with a time constant of 15 ps and is accompanied by 15% return to the ground state, 62% electron transfer to the L-side bacteriopheophytin, BPh(L), and 23% electron transfer to the M-side analogue, BPh(M). The data supporting electron transfer to the M-side include bleaching of the Q(X) band of BPh(M) at 528 nm and a spectrally and kinetically resolved anion band with a maximum at 640 nm assigned to BPh(M)(-). The decay of these features and concomitant approximately 20% decay of bleaching of the 850 nm band of P give a P(+)BPh(M)(-) lifetime on the order of 1-2 ns that reflects deactivation to give the ground state. These data and additional findings are compared to those from parallel experiments on the G(M201)D/L(M212)H double mutant, in which 15% electron transfer to BPh(M) has been reported previously and is reproduced here. We also compare the above results with the primary electron-transfer processes in S(L178)K, S(L178)K/G(M201)D, and S(L178)K /L(M212)H RCs and with those for the L(M212)H and G(M201)D single mutants and wild-type RCs. The comparison of extensive results that track the primary events in these eight RCs helps to elucidate key factors underlying the directionality and high yield of charge separation in the bacterial photosynthetic RC.
Asunto(s)
Bacterioclorofilas/metabolismo , Lisina/genética , Mutagénesis Sitio-Dirigida , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Sustitución de Aminoácidos/genética , Aniones/química , Ácido Aspártico/genética , Benzoquinonas/química , Transporte de Electrón/genética , Histidina/genética , Cinética , Complejos de Proteína Captadores de Luz , Feofitinas/química , Feofitinas/genética , Feofitinas/metabolismo , Fotoquímica , Proteínas del Complejo del Centro de Reacción Fotosintética/química , Rhodobacter capsulatus , Análisis EspectralRESUMEN
Qy-excitation resonance Raman (RR) studies are reported for a series of Rhodobacter capsulatus reaction centers (RCs) containing mutations at L-polypeptide residue 121 near the photoactive bacteriopheophytin (BPhL). The studies focus on the electronic/structural perturbations of BPhL induced by replacing the native Phe with an Asp residue. Earlier work has shown that the electron-transfer properties of F(L121)D RCs are closely related to those of RCs in which BPhL is replaced by bacteriochlorophyll (BChl) (beta-type RCs) or by pheophytin. In addition to the F(L121)D single mutant, RR studies were performed on the F(L121)D/E(L104)L double mutant, which additionally removes the hydrogen bond between BPhL and the native Glu L104 residue. The vibrational signatures of BPhL in the single and double mutants containing Asp L121 are compared with one another and with those of BPhL in both wild-type and F(L121)L RCs. The replacement of the aromatic Phe residue with Leu has no discernible effect on the vibrational properties of BPhL, a finding in concert with the previously reported absence of an effect of the mutation on the electron-transfer characteristics of the RC. In contrast, replacement of Phe with Asp significantly perturbs the vibrational characteristics of BPhL, and in a manner most consistent with Asp L121 being deprotonated and negatively charged. The negative charge of the carboxyl group of Asp L121 interacts with the pi-electron system of BPhL in a relatively nonspecific fashion, diminishing the contribution of charge-separated resonance forms of the C9-keto group to the electronic structure of the cofactor. The presence of a negative charge near BPhL is consistent with the known photochemistry of F(L121)D RCs, which indicates that the free energy of P+BPhL- is substantially higher than in wild-type RCs.
Asunto(s)
Ácido Aspártico/química , Feofitinas/química , Proteínas del Complejo del Centro de Reacción Fotosintética/química , Complejos de Proteína Captadores de Luz , Modelos Químicos , Rhodobacter capsulatus , Espectrometría RamanRESUMEN
The primary photochemistry in Rhodobacter capsulatus reaction centers (RCs) containing the Phe to Asp mutation at L polypeptide residue 121 near the photoactive bacteriopheophytin (BPhL) is characterized using ultrafast transient absorption spectroscopy. At 285 K, initial charge separation from P* proceeds with essentially unity quantum yield in approximately 6 ps to form a transient denoted P+I-. This transient is proposed to involve P+BPhL- and probably P+BChlL- as well (BChlL is the L-side bacteriochlorophyll molecule). P+I- decays in approximately 150 ps both by electron transfer to give P+QA- (approximately 78% yield) and by charge recombination to the ground state (approximately 22% yield). These results indicate that the F(L121)D mutant is closely related, in terms of its electron transfer properties, to previously reported RCs in which BPhL is replaced with a bacteriochlorophyll (beta-type RCs) or a pheophytin. However, the native BPhL pigment is retained in the F(L121)D mutant. We propose that the Asp at L121 raises the free energy of P+BPhL-, thereby giving rise to the altered photochemistry. At 77 K, the P+I- lifetime is shortened slightly to approximately 120 ps and the yield of P+QA- is increased to approximately 88%. This result is somewhat different from that obtained for beta-type RCs at low temperature, where the P+I- lifetime lengthens and the yield of P+QA- diminishes or stays about the same compared to the values near room temperature. We exploit these differences in developing a model for the charge separation process in the F(L121)D mutant. The effects of introducing an Asp near BPhL are compared to those obtained previously in two mutants in which an Asp is introduced near BChlL.
Asunto(s)
Ácido Aspártico , Proteínas del Complejo del Centro de Reacción Fotosintética/química , Cinética , Complejos de Proteína Captadores de Luz , Mutagénesis Sitio-Dirigida , Rhodobacter capsulatus , Espectrofotometría Atómica , Relación Estructura-ActividadRESUMEN
An aspartic acid residue has been introduced near ring V of the L-side accessory bacteriochlorophyll (BCHlL) or the photosynthetic reaction center in a rhodobacter capsulatus mutant in which a His also replaces Leu 212 on the M-polypeptide. The initial stage of charge separation in the G(M201)D/L(M212)H double mutant yields approximately 70 percent electron transfer to the L-side cofactors, approximately 15 percent rapid deactivation to the ground state, and approximately 15 percent electron transfer to the so-called inactive M-side bacteriopheophytin (BPhM). It is suggested here that the Asp introduced at M201 modulates the reduction potential of BCHlL, thereby changing the energetics of charge separation. The results demonstrate that an individual amino acid residue can, through its influence on the free energies of the charge-separated states, effectively dictate the balance between the forward electron transfer reactions on the L-side of the RC, the charge-recombination processes, and electron transfer to the M-side chromophores.
Asunto(s)
Bacterioclorofilas/metabolismo , Feofitinas/metabolismo , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Rhodobacter capsulatus/metabolismo , Bacterioclorofilas/química , Transporte de Electrón , Complejos de Proteína Captadores de Luz , Mutación , Feofitinas/química , Fotoquímica , Proteínas del Complejo del Centro de Reacción Fotosintética/química , Rhodobacter capsulatus/genética , Análisis Espectral , TermodinámicaRESUMEN
We report the initial characterization of a series of reaction centers (RCs) from the photosynthetic bacterium Rhodobacter capsulatus having single or double mutations of phenylalanines 97 and 121 on the L polypeptide. Substitution of these aromatic amino acids, which may interact with the photoactive bacteriopheophytin associated with the L polypeptide (BPhL), was carried out to examine their possible roles in electron transfer, charge stabilization, and/or BPhL binding. In some mutant RCs, the wild-type pigment content is obtained while in certain others a bacteriochlorophyll (BChL) replaces BPhL. The mutant RCs with wild-type pigment content are found to have overall photochemistry effectively identical to that of wild-type RCs. This indicates aromatic residues at L97 and L121 are not critical factors in the charge separation process, although an approximate 2-fold increase in the rate of electron transfer from BPhL- to QA is observed in two mutants where residue L121 is leucine. In two double mutants where L121 is histidine and L97 is either valine or cysteine, BPhL is replaced with a BChl (denoted beta). This pigment content is surprising since in the native RC structure amino acid L121 is not in optimum geometry for coordination to the Mg in the center of the pigment macrocycle. Charge separation takes place in the beta-containing mutants with an approximately 70% yield of P+QA- at 285 K compared to approximately 100% for wild-type. The photochemistry of these new beta-type RCs is very similar to that reported previously for the beta RC from Rhodobacter sphaeroides wherein the same pigment change was induced by a mutation in the M polypeptide.
Asunto(s)
Feofitinas/metabolismo , Proteínas del Complejo del Centro de Reacción Fotosintética/química , Sitios de Unión , Transporte de Electrón , Complejos de Proteína Captadores de Luz , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Fotoquímica , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Rhodobacter capsulatus/química , Análisis EspectralRESUMEN
1. Gender differences in the dietary regulation of rat liver glucose-6-P dehydrogenase activity, synthesis and mRNA levels were examined. 2. As expected, in normal rats fed a standard chow diet, females have higher G6PD activity than males because they have more G6PD mRNA and therefore a higher rate of G6PD synthesis. 3. In contrast, the decreased dietary induction in female rats is due to a more rapid rate of G6PD degradation rather than a decrease in G6PD mRNA or synthesis.
Asunto(s)
Glucosafosfato Deshidrogenasa/metabolismo , Homeostasis , Hígado/enzimología , Animales , Dieta , Ayuno , Femenino , Glucosafosfato Deshidrogenasa/biosíntesis , Glucosafosfato Deshidrogenasa/genética , Cinética , Masculino , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Caracteres SexualesRESUMEN
We have investigated the primary photochemistry of two symmetry-related mutants of Rhodobacter sphaeroides in which the histidine residues associated with the central Mg2+ ions of the two bacteriochlorophylls of the dimeric primary electron donor (His-L173 and His-M202) have been changed to leucine, affording bacteriochlorophyll (BChl)/bacteriopheophytin (BPh) heterodimers. Reaction centers (RCs) from the two mutants, (L)H173L and (M)H202L, have remarkably similar spectral and kinetic properties, although they are quite different from those of wild-type RCs. In both mutants, as in wild-type RCs, electron transfer to BPhL and not to BPhM is observed. These results suggest that asymmetry in the charge distribution of the excited BChl dimer (P*) in wild-type RCs (due to differing contributions of the two opposing intradimer charge-transfer states) contributes only modestly to the directionality of electron transfer. The results also suggest that differential orbital overlap of the two BChls of P with the chromophores on the L and M polypeptides does not contribute substantially to preferential electron transfer to BPhL.
Asunto(s)
Proteínas del Complejo del Centro de Reacción Fotosintética/química , Rhodobacter sphaeroides/metabolismo , Bacterioclorofilas/química , Transporte de Electrón , Complejos de Proteína Captadores de Luz , Fotoquímica , Rhodobacter sphaeroides/genética , Espectrofotometría , EstereoisomerismoRESUMEN
Conditions were identified which, for the first time, demonstrate that primary hepatocytes can express the same range of glucose-6-phosphate dehydrogenase (G6PD) synthesis and mRNA as in live rats. Primary hepatocytes were cultured without prior exposure to serum, hormones or carbohydrates. Five modulators implicated in G6PD induction in vivo were examined: insulin, dexamethasone, tri-iodothyronine (T3), glucose and fructose, T3 did not affect G6PD activity, and did not interact with carbohydrate to affect the activity of G6PD. Neither glucose nor fructose alone affected G6PD activity, and they did not interact with insulin to increase G6PD activity. Hepatocytes isolated from fasted rats and cultured in serum-free media with amino acids ad the only energy source how a 12-fold increase in G6PD synthesis and mRNA (measured by a solution-hybridization assay). This induction does not require added hormones or carbohydrate. The addition of insulin alone caused another increase in G6PD synthesis and mRNA. There are at least three distinct phases to G6PD induction under these conditions. The largest increase in G6PD synthesis (12-fold) occurs in the absence of any hormones and with amino acids as the only energy source. This phase is due to increased G6PD mRNA. Insulin causes an additional 2-3-fold increase in G6PD synthesis and mRNA. However, dexamethasone and insulin are both required before G6PD synthesis is equal to that in rats which are fasted and refed on a high-carbohydrate diet.
Asunto(s)
Glucosafosfato Deshidrogenasa/metabolismo , Hígado/enzimología , ARN Mensajero/metabolismo , Animales , Células Cultivadas , Dexametasona/farmacología , Inducción Enzimática , Fructosa/farmacología , Regulación Enzimológica de la Expresión Génica , Glucosafosfato Deshidrogenasa/biosíntesis , Glucosafosfato Deshidrogenasa/genética , Insulina/farmacología , Cinética , Hígado/efectos de los fármacos , ARN Mensajero/genética , Ratas , Ratas Endogámicas , Triyodotironina/farmacologíaRESUMEN
Site-directed mutagenic replacement of M subunit Leu214 by His in the photosynthetic reaction center (RC) from Rhodobacter sphaeroides results in incorporation of a bacteriochlorophyll molecule (BChl) in place of the native bacteriopheophytin (BPh) electron acceptor. Evidence supporting this conclusion includes the ground-state absorption spectrum of the (M)L214H mutant, pigment and metal analyses, and time-resolved optical experiments. The genetically modified RC supports transmembrane charge separation from the photoexcited BChl dimer to the primary quinone through the new BChl molecule, but with a reduced quantum yield of 60 percent (compared to 100 percent in wild-type RCs). These results have important implications for the mechanism of charge separation in the RC, and rationalize the choice of (bacterio)pheophytins as electron acceptors in a variety of photosynthetic systems.
Asunto(s)
Bacterioclorofilas/metabolismo , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Rhodobacter sphaeroides/metabolismo , Transporte de Electrón , Histidina , Cinética , Leucina , Complejos de Proteína Captadores de Luz , Mutagénesis Sitio-Dirigida , Feofitinas/metabolismo , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , EspectrofotometríaRESUMEN
Subpicosecond time-resolved photodichroism measurements on Rhodobacter sphaeroides R26 reaction centers are reported in the key region between 620 and 740 nm, where the anions of both bacteriopheophytin and bacteriochlorophyll (BChl) have their most diagnostic absorption bands. These measurements fail to resolve clearly the formation of a reduced BChl species. The implications of this for elucidating the role of the accessory BChl in the initial stage of charge separation are discussed.
Asunto(s)
Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Rhodobacter sphaeroides/metabolismo , Bacterioclorofilas/metabolismo , Transporte de Electrón , Cinética , Complejos de Proteína Captadores de Luz , Feofitinas/metabolismo , Espectrometría RamanRESUMEN
A solution hybridization assay is systematically characterized and used to quantitate glucose-6-phosphate dehydrogenase (G6PD) mRNA from epididymal fat pads in fasted and glucose-induced rats. G6PD mRNA and specific activity increase 9-fold and 2-fold, respectively. The 9-fold increase in G6PD synthesis reported previously (Wolfe et al. (1979) Biochem. Biophys. Res. Commun. 89, 108-115) can, therefore, be accounted for by the increase in G6PD mRNA. This solution hybridization assay is sensitive enough to quantitative levels of G6PD mRNA in total liver RNA from a fasted rat, one of the least abundant sources of this mRNA. It can, therefore, be used to answer several questions about the regulation of G6PD synthesis in rat tissues. Preliminary results suggest that the dietary regulation of G6PD mRNA in rat liver is much larger than previously reported.
Asunto(s)
Tejido Adiposo/enzimología , Epidídimo/enzimología , Glucosafosfato Deshidrogenasa/genética , ARN Mensajero/análisis , Animales , Northern Blotting , Sondas de ADN , Glucosafosfato Deshidrogenasa/metabolismo , Masculino , Hibridación de Ácido Nucleico , Ratas , Ratas Endogámicas , SolucionesRESUMEN
Rat liver glucose-6-phosphate dehydrogenase (G6PD) is one of several proteins involved in lipid metabolism whose synthesis is regulated by diet. In experiments reported here, rats were fasted or fed diets until a new steady state level of G6PD was produced. Livers were used to measure G6PD activity, synthesis and mRNA simultaneously. Since accurate quantitation of G6PD mRNA by Northern blots was found to be difficult in noninduced animals a new solution hybridization assay was also used. Noninduced rats have approx. One molecule of G6PD mRNA per liver cell. Changes in G6PD mRNA are larger than previously reported and, at the steady state, can completely account for the 33-fold change in G6PD activity and synthesis when fasted rats are refed a high carbohydrate diet. In contrast, a high fat carbohydrate-free diet does not increase G6PD mRNA and dibutyryl cAMP lowers G6PD mRNA. Since changes in G6PD synthesis and activity are closely correlated, degradation of G6PD is not significantly regulated.
Asunto(s)
Glucosafosfato Deshidrogenasa/genética , Hígado/metabolismo , ARN Mensajero/biosíntesis , Animales , Northern Blotting , Bucladesina/farmacología , Dieta , Hibridación de Ácido Nucleico , Ratas , Ratas EndogámicasRESUMEN
The rates of the primary electron-transfer processes in Rhodobacter sphaeroides reaction centers have been examined in detail by using 150-fs excitation flashes at 870 nm. At room temperature the apparent time constants for both initial charge separation (P* --> P+BPhL-) and subsequent electron transfer (P+BPhL- --> P+QA-) are found to encompass a range of values (approximately 1.3-4 ps and approximately 100-320 ps, respectively), depending on the wavelength at which the kinetics are followed. We suggest this reflects a distribution of reaction centers (or a few conformers), having differences in factors such as distances or orientations between the cofactors, hydrogen bonding, or other pigment-protein interactions. We also suggest that the time constants observed at cryogenic temperatures (approximately 1.3 and approximately 100 ps, respectively, with much smaller or negligible variation with detection wavelength) do not reflect an actual increase in the rates with decreasing temperature but rather derive from a shift in the distribution of reaction centers toward those in which electron transfer inherently occurs with the faster rates.
RESUMEN
Rats were fasted or fasted and refed simple purified diets so the effects of individual carbohydrates or fats could be studied. Freshly isolated hepatocytes from these animals were used to measure both apoE synthesis and mRNA levels so any changes in apoE synthesis that might occur without changes in its mRNA could be detected. Some of these experiments were done with both sexes. Both fasting and fasting and refeeding a 60% glucose fat-free diet significantly increased spoE synthesis. However, cyclic AMP is not likely to rapidly mediate the effect of fasting since dibutyryl cAMP slightly lowered (rather than increased) apoE synthesis and mRNA when injected into rats for 4.5 h. Dietary fat had no effect either in the absence of carbohydrate or when consumption of carbohydrate was constant in pair-fed rats. ApoE mRNA levels remained normal for 4 days in primary hepatocytes cultured in medium that had only amino acids as an energy source. Added hormones or fructose had no significant effect. Thus, only fasting and fasting and refeeding glucose were able to significantly change apoE synthesis or mRNA levels. Synthesis of apoE may be regulated to increase when apoE is secreted with very low density lipoprotein or when apoE in secreted high density lipoprotein is needed to acquire cholesteryl esters for the synthesis of bile salts and acids by liver.
Asunto(s)
Apolipoproteínas E/biosíntesis , Dieta , Hormonas/farmacología , ARN Mensajero/metabolismo , Animales , Dexametasona/farmacología , Grasas de la Dieta/administración & dosificación , Ayuno , Femenino , Insulina/farmacología , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Ratas , Ratas EndogámicasRESUMEN
Time-resolved optical measurements encompassing the femtoseconds to seconds time scales have been used to investigate Rhodobacter capsulatus reaction centers (RCs) in which the histidine residue at position 200 on the M polypeptide has been changed to a leucine by site-directed mutagenesis. The HisM200----Leu RC, which has a heterodimer consisting of a bacteriochlorophyll and a bacteriopheophytin, is capable of the primary photochemistry observed in wild-type Rb. capsulatus RCs, but with an overall quantum yield reduced by about half. The lower yield resides in the initial electron transfer reaction and may be associated in part with substantial charge transfer character of the excited heterodimer. These and other comparisons between Rb. capsulatus wild-type and HisM200----Leu RCs have important implications for our understanding of the mechanism of electron transfer in the RC and the efficiency of the charge separation process.