RESUMEN
SUMMARYStaphylococcus aureus is a leading cause of infections in deployed service members. Based on a molecular epidemiological study of 182 MRSA isolates from patients in three U.S. Army combat support hospitals in separate regions in Iraq, USA300 clone was the most predominant (80%) pulsotype. This finding suggested that strain carriage from the home country by military personnel is epidemiologically more important than local acquisition.
Asunto(s)
Infección Hospitalaria/epidemiología , Hospitales Militares/estadística & datos numéricos , Staphylococcus aureus Resistente a Meticilina , Infecciones Estafilocócicas/epidemiología , Infección Hospitalaria/microbiología , Genotipo , Humanos , Irak/epidemiología , Guerra de Irak 2003-2011 , Staphylococcus aureus Resistente a Meticilina/genética , Epidemiología Molecular , Infecciones Estafilocócicas/microbiologíaAsunto(s)
Cromosomas Humanos Par 4 , Polimorfismo de Longitud del Fragmento de Restricción , Secuencias Repetitivas de Ácidos Nucleicos/genética , Secuencia de Bases , Southern Blotting , ADN de Cadena Simple/genética , Desoxirribonucleasas de Localización Especificada Tipo II/metabolismo , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Población BlancaAsunto(s)
Cromosomas Humanos Par 4 , Polimorfismo Genético , Secuencias Repetitivas de Ácidos Nucleicos , Secuencia de Bases , Sondas de ADN , Frecuencia de los Genes , Humanos , Datos de Secuencia Molecular , Oligodesoxirribonucleótidos , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de RestricciónAsunto(s)
Reacción en Cadena de la Polimerasa/métodos , Secuencias Repetitivas de Ácidos Nucleicos , Alelos , Secuencia de Bases , Southern Blotting , Sondas de ADN/genética , Frecuencia de los Genes , Humanos , Masculino , Datos de Secuencia Molecular , Oligodesoxirribonucleótidos , Polimorfismo de Longitud del Fragmento de Restricción , Cromosoma XRESUMEN
The highly polymorphic VNTR locus pYNZ32 has been more extensively characterized, and its analysis converted to a rapid PCR-based format. DNA sequencing in the areas within and flanking the repeated segment allowed the design of specific amplification primers. The repeated region of pYNZ32 consists of an imperfectly duplicated 27-bp motif, 16 bases of which are more highly conserved. Allelic products from PCR amplification were resolved into nine different size classes ranging from approximately 1400 to 2200 bp. Additional polymorphism was revealed when the amplified products were analyzed by restriction enzyme digestion. Both the overall size variation and the internal sequence polymorphism were used to determine a heterozygosity value of 86% for YNZ32 in 50 unrelated individuals. The rapid analysis and improved resolution of amplified alleles on agarose gels, and the internal variability within YNZ32, increase its diagnostic utility as a VNTR and as a linkage marker for the nearby Huntington disease gene.
Asunto(s)
Ligamiento Genético , Enfermedad de Huntington/genética , Secuencias Repetitivas de Ácidos Nucleicos/genética , Secuencia de Bases , ADN , Heterocigoto , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Mapeo RestrictivoAsunto(s)
Fibrosis Quística/genética , Secuencia de Aminoácidos , Secuencia de Bases , Regulador de Conductancia de Transmembrana de Fibrosis Quística , ADN/genética , Análisis Mutacional de ADN , Femenino , Humanos , Masculino , Proteínas de la Membrana/genética , Biología Molecular , Datos de Secuencia Molecular , LinajeRESUMEN
A 3-bp deletion (delta F508) in the cystic fibrosis (CF) gene is the mutation on the majority of CF chromosomes. We studied 112 CF families from North American populations of French ancestry: French-Canadian families referred from hospitals in three cities in Quebec and from the Saguenay-Lac St. Jean region of northeastern Quebec and Acadian families living in Louisiana. delta F508 was present on 71%, 55%, and 70% of the CF chromosomes from the major-urban Quebec, Saguenay-Lac St. Jean, and Louisiana Acadian families, respectively. A weighted estimate of the proportion of delta F508 in the French-Canadian patient population of Quebec was 70%. We found that 95% of the CF chromosomes with delta F508 had D7S23 haplotype B, the most frequent haplotype on CF chromosomes. In the Saguenay-Lac St. Jean families, 86% of the CF chromosomes without delta F508 had the B haplotype, compared with 31% for the major-urban Quebec and Louisiana Acadian families. The incidence of CF in the Saguenay-Lac St. Jean population was 1/895 live-born infants.
Asunto(s)
Fibrosis Quística/genética , Mutación , Southern Blotting , Deleción Cromosómica , Fibrosis Quística/etnología , Sondas de ADN , Femenino , Francia/etnología , Frecuencia de los Genes , Ligamiento Genético/genética , Haplotipos , Humanos , Incidencia , Louisiana , Masculino , QuebecRESUMEN
Six genetic polymorphisms, closely linked to the cystic fibrosis gene and useful in clinical linkage analysis, have been characterized and converted to a more rapid form of assay. Sequences flanking the metD (Ban I), metH (Msp I), XV-2c (Taq I), KM.19 (Pst I), MP6d-9 (Msp I), and J3.11 (Msp I) polymorphic restriction sites have been determined and used to design specific polymerase chain reaction (PCR) amplification primers and allele-specific oligonucleotide probes. All six of these polymorphisms were found to involve single-base alterations, and the XV-2c polymorphism was found to lie within an Alu repeat segment. These PCR-based tests, in conjunction with the CS.7 (Hha I) assay described elsewhere (Stanier P et al. Hum Genet 1988;80:309-10; Williams C et al. Lancet 1988;ii:102-3), provide a convenient, rapid, and reliable method of haplotype and linkage analysis, clinically useful in those situations where direct detection of mutations is not possible.