Enhancing soil health and strawberry disease resistance: the impact of calcium cyanamide treatment on soil microbiota and physicochemical properties.

Introduction: Continuous strawberry cropping often causes soil-borne diseases, with 20 calcium cyanamide being an effective soil fumigant, pig manure can often be used as soil organic fertilizer. Its impact on soil microorganisms structure, however, remains unclear. Methods: This study investigated the effectiveness of calcium cyanamide and pig manure in treating strawberry soil, specifically against strawberry anthracnose. We examined the physical and chemical properties of the soil and the rhizosphere microbiome and performed a network analysis. Results: Results showed that calcium cyanamide treatment significantly reduces the mortality rate of strawberry in seedling stage by reducing pathogen abundance, while increasing actinomycetes and Alphaproteobacteria during the harvest period. This treatment also enhanced bacterial network connectivity, measured by the average connectivity of each Operational Taxonomic Unit (OTU), surpassing other treatments. Moreover, calcium cyanamide notably raised the levels of organic matter, available potassium, and phosphorus in the soil-key factors for strawberry disease resistance and yield. Discussion: Overall, applying calcium cyanamide to soil used for continuous strawberry cultivation can effectively decrease anthracnose incidence. It may be by changing soil physical and chemical properties and enhancing bacterial network stability, thereby reducing the copy of anthracnose. This study highlights the dual benefit of calcium cyanamide in both disease control and soil nutrient enhancement, suggesting its potential as a valuable tool in sustainable strawberry farming.

Identification of tumor-specific neoantigens and immune clusters of hepatocellular carcinoma for mRNA vaccine development.

BACKGROUND: To screen efficacious neoantigens for the development of LIHC mRNA vaccines, construct LIHC immune clusters, and therefore select patients who might benefit from vaccination. METHODS: RNA-seq data and clinical information of 371 TCGA-LIHC and 231 ICGC-LIHC cohorts were downloaded. Differentially expressed genes and their associations with prognosis were analyzed by GEPIA, genetic alterations were examined in the cBioPortal portal, and the association between genes and immune infiltrating cells was explored by TIMER. The immune clusters were constructed by consistency clustering, and the immune landscape was described using CIBERSORT. RESULTS: POLR3C and KPNA2 were identified as LIHC tumor neoantigens related to inferior prognosis and antigen-presenting cell infiltration. In addition, three immune clusters (IC1, IC2 and IC3) with significant differences in molecular, immune cytological, and clinical features were identified in both the TCGA and ICGC LIHC cohorts. Immune "hot" phenotype IC3 displayed a better survival than IC2, and immune "cold" phenotype IC1 exhibited a high tumor mutation burden. CONCLUSION: In conclusion, for the development of anti-LIHC mRNA vaccines, we identified efficacious neoantigens POLR3C and KPNA2, profiled the tumor microenvironment of LIHC, and identified IC1 patients as the subgroup who might not most benefit from vaccination.

Observation of the effect of arthroscopically assisted percutaneous minimally invasive Kirschner wire tension band fixation on the treatment of patellar fractures.

BACKGROUND: Patellar fractures are common intra-articular fractures. Arthroscopically assisted reduction and fixation has been shown to advantageous in the treatment of patellar fractures. OBJECTIVE: This study aimed to investigate the effect of arthroscopically assisted percutaneous minimally invasive Kirschner wire tension band fixation on the treatment of patellar fractures. METHODS: Thirty-four patients with acute closed patellar fractures enrolled in the study and were managed by arthroscopically assisted percutaneous minimally invasive Kirschner wire tension band fixation under epidural anesthesia. Among these patients, 24 were males, and ten were females. The mean age of these patients was 38 ± 0.8 years (range: 25-62 years). Of these patients, 20 had transverse fractures, five had longitudinal fractures, four had marginal oblique fractures, and five had multifragmentary upper and lower pole. Fracture causes: 25 were caused by falls, and nine were caused by traffic accidents. The time from injury to operation was 6-48 hours, with an average of 23 hours. The average post-operative follow-up was 12.8 months, and at the end of follow-up, the Boestman scoring system was used to evaluate knee joint function. RESULTS: All the wounds healed by first intention, and no infections, skin necrosis, joint stiffness, or other complications occurred. Three months after operation, the tension band wire had broken in one patient, but the fracture had united, and this did not affect the prognosis. In eight patients, the average circumference at 10 cm above the patella was 0.6 cm less on the injured side than on the contralateral side. The average Boestman score was 27.5. The final result was excellent in 25 patients, good in eight patients, and acceptable in one patient. CONCLUSION: Arthroscopic management of simple patellar fracture with percutaneous minimally invasive Kirschner wire tension band fixation is less invasive than open operation, while still leading to firm fixation, and is conducive to early functional training and functional recovery, and thus is an effective method of management for patellar fractures.

Assembly of functional microbial communities in paddy soil with long-term application of pig manure under rice-rape cropping system.

Organic farming is considered an efficient approach to improve soil fertility for sustainable agriculture. However, its soil micro-ecological effects and functions in intensive rice cropping systems are still obscure. Twelve soil samples were collected from a field experiment with four treatments such as M0 (no pig manure), M1 (1.6 t ha-1 pig manure), M2 (3.2 t ha-1 pig manure) and M3 (4.8 t ha-1 pig manure) after eight rice-oilseed rape rotation. Soil chemical property, enzyme activity and abundant/rare bacterial or fungal communities were analyzed to investigate the effect of conversion to organic farming with continuous pig manure application on soil microbiota. Stochastic processes controlled the assembly of abundant taxa, and deterministic processes dominated rare taxa. The composition and network construction of bacterial and fungal communities were significantly affected by pig manure, with changes in soil property and enzyme activity. Based on partial least squares path modeling (PLS-PM), pig manure application affected bacteria construction and enzyme activities by increasing soil carbon (C) and nitrogen (N). In summary, long-term pig manure application promotes specific microbial associations known to be involved in degrading complex organic compounds, and improving soil fertility such as soil enzyme activities. This research provides insight into understanding the processes behind changes in bacterial and fungal communities in paddy soil after conversion to organic farming.

Foliar application of glycinebetaine and Zn fertilizer improves both the apparent and functional qualities of albino tea [Camellia sinensis (L.) O. Kuntze].

With Zn deficiency increasing in the global population, functional plant food (including tea) can help to fill the nutrition gap that the main crops cannot meet. Glycinebetaine (GB), an important bioactive substance with a wide range of natural sources, has received limited attention towards its effects on Zn biofortification and the quality of tea. The Zn enrichment and metabolite responses of albino tea [cv. White leaf No. 1 (WL-1)] to the foliar application of GB, Zn, and their combination (Zn + GB) were investigated in a field experiment. The result indicated that the 100-buds weight, total N, Zn, Thea, and total amino acid content in the young leaves of WL-1 with Zn2 + GB2 treatment were significantly increased, whereas the Chla contents were decreased (p < 0.05). The total catechins and CAF contents of Zn2 + GB2 treatment were lower than those of other treatments, with significance (p < 0.05). Multivariate analysis and general quantitative analysis returned complementary results, revealing that Zn2 + GB2 treatment was better for the apparent and functional quality of WL-1. The more theanine and Zn, limited chlorophyll, catechin, and caffeine contributed to the quality improvement, as well as to maintaining the leaf albinistic characteristics, inhibiting astringency and bitterness, exerting flavor and umami, and improving the ultimate beneficial functions. The combined application of Zn and GB is a promising practice for Zn biofortification and for the quality improvement of tea, with spraying 750 L ha-1 of 2.0 g L-1 Zn fertilizer and 3.2 g L-1 GB mixture recommended.

TBC1D3 promotes neural progenitor proliferation by suppressing the histone methyltransferase G9a.

Genomic changes during human linage evolution contribute to the expansion of the cerebral cortex to allow more advanced thought processes. The hominoid-specific gene TBC1D3 displays robust capacity of promoting the generation and proliferation of neural progenitors (NPs), which are thought to contribute to cortical expansion. However, the underlying mechanisms remain unclear. Here, we found that TBC1D3 interacts with G9a, a euchromatic histone lysine N-methyltransferase, which mediates dimethylation of histone 3 in lysine 9 (H3K9me2), a suppressive mark for gene expression. TBC1D3 displayed an inhibitory role in G9a's histone methyltransferase activity. Treatment with G9a inhibitor markedly increased NP proliferation and promoted human cerebral organoid expansion, mimicking the effects caused by TBC1D3 up-regulation. By contrast, blockade of TBC1D3/G9a interaction to disinhibit G9a caused up-regulation of H3K9me2, suppressed NP proliferation, and impaired organoid development. Together, this study has demonstrated a mechanism underlying the role of a hominoid-specific gene in promoting cortical expansion.

Identification and quantitative chemical analysis of betaines in different organic wastes and their bioconversion composts.

Global organic waste is increasing, bioconversion of organic waste arises because it can recover valuable nutrients and produce bioactive substances. Betaines are important bioactive substances in plants under environmental stress, but have received limited attention in vermicompost/larvae bioconversion compost. In this study, betaines in organic waste and vermicompost/larvae bioconversion compost were identified and quantified by HPLC-ESI-MS/MS. We observed the existence of glutamine betaine in all samples, which was first found in natural sources recently. Valine betaine was the highest among all detected betaines followed by GABA betaine, and both were rare in plants. The existence of tyrosine betaine in cow dung (CD) and vermicompost (CDV) was found, which was previously shown to be in fungi. Most importantly, we found larvae bioconversion could increase betaines by 5.56-99.75%, while vermicomposting decreased them. Bioconversion of larvae can effectively increase betaines in compost and can be used to produce potential novel functional organic fertilizers.

Successive phytoextraction alters ammonia oxidation and associated microbial communities in heavy metal contaminated agricultural soils.

Phytoextraction is an attractive strategy for remediation of soils contaminated by heavy metal (HM), yet the effects of this practice on biochemical processes involved in soil nutrient cycling remain unknown. Here we investigated the impact of successive phytoextraction with a Cd/Zn co-hyperaccumulator Sedum alfredii (Crassulaceae) on potential nitrification rates (PNRs), abundance and composition of nitrifying communities and functional genes associated with nitrification using archaeal and bacterial 16S rRNA gene profiling and quantitative real-time PCR. The PNRs in rhizosphere were significantly (P < 0.05) lower than in the unplanted soils, and decreased markedly with planting time. The decrease of PNR was more paralleled by changes in numbers of copy and transcript of archaeal amoA gene than the bacterial counterpart. Phylogenetic analysis revealed that phytoextraction induced shifts in community structure of soil group 1.1b lineage-dominated ammonia-oxidizing archaea (AOA), Nitrosospira cluster 3-like ammonia-oxidizing bacteria (AOB) and Nitrospira-like nitrite-oxidizing bacteria (NOB). A strong positive correlation was observed between amoA gene transcript numbers and PNRs, whereas root exudates showed negative effect on PNR. This effect was further corroborated by incubation test with the concentrated root exudates of S. alfredii. Partial least squares path model demonstrated that PNR was predominantly controlled by number of AOA amoA gene transcripts which were strongly influenced by root exudation and HM level in soil. Our result reveals that successive phytoextraction of agricultural soil contaminated by HMs using S. alfredii could inhibit ammonia oxidation and thereby reduce nitrogen loss.

[Molecular and cellular mechanisms of cortical expansion and folding in brain development and evolution].

During the evolution from primates to humans, the size of cerebral cortex is increased by forming more gyri and sulci, which is believed to be highly associated with cognitive abilities and the basis of higher brain functions in humans. Accumulating lines of evidence have shown that the cortical size is regulated both by protein-coding genes and non-coding RNAs. In particular, the recently identified outer radial glial cells (oRGs) distributed in the outer subventricular zone (oSVZ) of gyrencephalic brains, have been considered to be important for cortical expansion and folding. This review summarizes recent progresses in the understanding of cortex expansion and discusses the potential molecular and cellular mechanisms of cortical folding.

The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice.

Cortical expansion and folding are often linked to the evolution of higher intelligence, but molecular and cellular mechanisms underlying cortical folding remain poorly understood. The hominoid-specific gene TBC1D3 undergoes segmental duplications during hominoid evolution, but its role in brain development has not been explored. Here, we found that expression of TBC1D3 in ventricular cortical progenitors of mice via in utero electroporation caused delamination of ventricular radial glia cells (vRGs) and promoted generation of self-renewing basal progenitors with typical morphology of outer radial glia (oRG), which are most abundant in primates. Furthermore, down-regulation of TBC1D3 in cultured human brain slices decreased generation of oRGs. Interestingly, localized oRG proliferation resulting from either in utero electroporation or transgenic expression of TBC1D3, was often found to underlie cortical regions exhibiting folding. Thus, we have identified a hominoid gene that is required for oRG generation in regulating the cortical expansion and folding.

[Light and temperature indices during the seeding stage of spring maize in Hetao irrigation district, Inner Mongolia, China].

We collected the experimental dataset of a spring-maize in Hetao irrigation district (Bayannaoer, Inner Mongolia) during 2012 and 2013, as well as the crop data observed in related agrometeorological stations to investigate the impacts of light and temperature variation on maize development during the seedling stage to derive adequate light and temperature indices for different suitability. Furthermore, polynomial fitting was applied to interpolate the temperature indices for each day of the whole seedling period to draw the dynamics of the temperature indices and to better characterize its influence on maize development during the seedling stage. The results suggested that the emergence and development rates increased by 11% and 12%, respectively when the average temperature increased 1 °C during the sowing-emergence period and emergence-jointing period, and the biological lowest temperature was 7.4 and 11.9°C , respectively. The optimum temperature at the sowing-emergence stage ranged from 16.0 to 18.0°C , and the leaf area index at the three-leaf stage reached 0.0172 and above. The optimum temperature at the emergence-jointing stage ranged from 21.6 to 23.0°0C , and the photosynthesis/growth of plant tissue was positively correlated with the temperature. The leaf area index in the jointing period could reach 2.15 and the plant height was 120 cm. At the emergence-jointing stage, when the sunshine percentage 74%, or the daily sunshine hours ≥11.0 h . d-1 , or the total sunshine hours ≥540 h, the dry mass of plant tissue could

[Agricultural climate regionalization of dryland farming for potato in Yinshan based on GIS].

Based on the meteorology dataset of 34 stations over the Yinshan area through the recent 30 years (1982-2010), we investigated the key environmental variables influencing potato yield using the correlation and regression methods. Two environmental variables, including the mean temperature difference, precipitation during the growing season, were selected as the major indexes for determining the suitable area for planting potato. Using the GIS-based small grid calculation model, we interpolated these two major environmental variables and produced the climatic map for potato in Yinshan area. The results showed the high potato yield area located in Qianshan and southern Houshan, and the medium-yield division was mainly concentrated in the central Houshan and north-west Qianshan, the low-yield division was distributed mainly in northern Yinshan. Moreover, this study examined the spatial patterns of potato production, and evaluated the stability of potato yield by combining the relative variability of potato yield. This study could provide valuable references for planting potato in Yinshan area.

Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

Axon guidance (pathfinding) wires the brain during development and is regulated by various attractive and repulsive cues. Semaphorin 3A (Sema3A) is a repulsive cue, inducing the collapse of axon growth cones. In the mammalian forebrain, the corpus callosum is the major commissure that transmits information flow between the two hemispheres, and contralateral axons assemble into well-defined tracts. We found that the patterning of callosal axon projections in rodent layer II and III (L2/3) cortical neurons in response to Sema3A was mediated by the activation of Rab5, a small guanosine triphosphatase (GTPase) that mediates endocytosis, through the membrane fusion protein Rabaptin-5 and the Rab5 guanine nucleotide exchange factor (GEF) Rabex-5. Rabaptin-5 bound directly to Plexin-A1 in the Sema3A receptor complex [an obligate heterodimer formed by Plexin-A1 and neuropilin 1 (NP1)]; Sema3A enhanced this interaction in cultured neurons. Rabaptin-5 bridged the interaction between Rab5 and Plexin-A1. Sema3A stimulated endocytosis from the cell surface of callosal axon growth cones. In utero electroporation to reduce Rab5 or Rabaptin-5 impaired axon fasciculation or caused mistargeting of L2/3 callosal projections in rats. Overexpression of Rabaptin-5 or Rab5 rescued the defective callosal axon fasciculation or mistargeting of callosal axons caused by the loss of Sema3A-Plexin-A1 signaling in rats expressing dominant-negative Plexin-A1 or in NP1-deficient mice. Thus, our findings suggest that Rab5, its effector Rabaptin-5, and its regulator Rabex-5 mediate Sema3A-induced axon guidance during brain development.

Hydroxy-terminated conjugated polymer nanoparticles have near-unity bright fraction and reveal cholesterol-dependence of IGF1R nanodomains.

Fluorescent nanoparticles have enabled many discoveries regarding how molecular machines function. Quantum dots have been the dominant class of fluorescent nanoparticles but suffer from blinking and from a substantial dark fraction--particles where the fluorescence is never seen--complicating any analysis of biological function. Nanoparticles composed of conjugated fluorescent polymers (Pdots) have recently been shown to have high brightness and no blinking. Here we develop a robust and efficient means to measure the dark fraction of Pdots, conjugating Atto dyes to the nanoparticles and testing fluorescence colocalization of dye and Pdot puncta. This established that the Pdots we generated had minimal dark fraction: ∼3%. The application of nanoparticles in biological environments is highly sensitive to surface functionalization. For Pdots we found that passivation with uncharged hydroxy-terminated polyethylene glycol caused a dramatic reduction in nonspecific cell binding and aggregation compared to a charged coating. Using carbonyl di-imidazole the hydroxy-Pdots were functionalized efficiently with streptavidin for high stability targeting, allowing specific labeling of mammalian cells. Type I insulin-like growth factor receptor (IGF1R) regulates cell survival and development, with roles in aging, heart disease, and cancer. We used hydroxy-Pdots to track the dynamics of IGF1R on a breast cancer cell-line, determining the diffusion characteristics and showing cholesterol-containing membrane nanodomains were important for receptor mobility at the plasma membrane. The near-unity bright fraction and low nonspecific binding of hydroxy-Pdots, combined with Pdot photostability and lack of blinking, provides many advantages for investigations at the single molecule level.

[Macroscopical anatomic structure of acupoint "Shangyang" (LI 1), "Erjian" (LI 2), "Sanjian" (LI 3), "Hegu" (LI 4), and "Quchi" (LI 11) regions in the forelimb in rabbits].

OBJECTIVE: To observe the structure of acupoints: "Shangyang" (LI 1), "Erjian" (LI 2), "Sanjian" (LI 3), "Hegu" (LI 4) and "Ouchi" (LI 11) of the Yangming Meridian in the rabbit's forelimb. METHODS: The acupoints were first located in accordance with the related atlas of rabbits and confirmed by low resistance and higher electric potential determinations by using a "multipurpose electronic acupoint detection and therapeutic instrument". Then the animals under anesthesia were killed by intravenous injection of air embolism, perfused with 5% natrium citricum first, acetic ether containing acrylonitrile-butadiene-styrene copolymer, and 5% dicapryl phthalate, blue and red pigments for paintings via subclavian artery, respectively. After routinely inserting an acupuncture needle into the acupoint, the local tissues (muscles, blood vessels and nerves) of the acupoints mentioned above were dissected layer by layer and their relations with the needle were observed under microscope. RESULTS: "Erjian" (LI 2) is situated at the depression site distal to the second metacarpophalangeal joint on the radial side. "Sanjian" (LI 3) is located at the depression site proximal to the second metacarpophalangeal joint on the radial side. The shallow-layers of "Shangyang" (LI 1), "Erjian" (LI 2), "Sanjian" (LI 3), "Hegu" (LI 4) and "Quchi" (LI 11) mainly contain cephalic vein and shallow branches of the radial nerve, and their deep layers chiefly contain radial artery and its branches, and the median nerve. CONCLUSION: "Shangyang" (LI 1), "Erjian" (LI 2), "Sanjian" (LI 3), "Hegu" (LI 4) and "Quchi" (LI 11) have a close association with the cephalic vein,radial artery,radial vein and its branches,superficial branch of radial nerve and the median nerve,which constitutes the morphological basis of the five points.

Mononuclear-dinuclear equilibrium of grafted copper complexes confined in the nanochannels of MCM-41 silica.

Following the structural concept of copper-containing proteins in which dinuclear copper centers are connected by hydroxide bridging ligands, a bidentate copper(II) complex has been incorporated into nano-confined MCM-41 silica by a multistep sequential grafting technique. Characterization by a combination of EPR spectroscopy, X-ray photoelectron spectroscopy (XPS), UV/Vis spectroscopy, IR spectroscopy , and solid-state (13)C and (29)Si cross-polarization magic-angle spinning (CP-MAS) NMR suggests that dinuclear Cu complexes are bridged by hydroxide and other counterions (chloride or perchlorate ions), similar to the situation for EPR-undetectable [Cu(II)···Cu(II)] dimer analogues in biological systems. More importantly, a dynamic mononuclear-dinuclear equilibrium between different coordination modes of copper is observed, which strongly depends on the nature of the counterions (Cl(-) or ClO(4)(-)) in the copper precursor and the pore size of the silica matrix (the so-called confinement effect). A proton-transfer mechanism within the hydrogen-bonding network is suggested to explain the dynamic nature of the dinuclear copper complex supported on the MCM-41 silica.

Comparative study on polymer light-emitting devices based on blends of polyfluorene and 4,7-di-2-thienyl-2,1,3-benzothiadiazole with devices based on copolymer of the same composition.

The small molecule 4,7-di-2-thienyl-2,1,3-benzothiadiazole (DBT) dye has been synthesized and blended into blue-emitting polyfluorene as a host. The photophysics of the blend films and electroluminescence device performance was compared with that from polyfluorene-co-4,7-di-2-thienyl-2,1,3-benzothiadiazole (PFO-DBT) copolymer of the same molar composition, with DBT incorporated covalently into the polyfluorene backbone. The role of intra- and interpolymer chain interaction and energy transfer in the polymer light-emitting devices are discussed. On the basis of the direct comparison of blend and copolymer from the same molecular composition, we can draw the conclusion that, in conjugated polymers, intrachain energy transfer along the polymer backbone is more efficient than that via interchain interaction and plays a more important role in polymer light-emitting devices.