Detection of synovial fluid LTF and S100A8 by chemiluminescence immunoassay for the diagnosis of periprosthetic joint infection.

BACKGROUND AND AIMS: Synovial fluid lactoferrin (LTF) and S100 calcium-binding protein A8 (S100A8) have been considered as potential biomarkers for the diagnosis of periprosthetic joint infection (PJI) through our previous research. However, the detection methods of these two proteins are still immature, so a rapid, accurate and cost-effective testing method is warranted. MATERIALS AND METHODS: We developed chemiluminescent immunoassays (CLIA) for the automated detection of synovial fluid LTF and S100A8 and assessed the analytical performance for these two methods. In addition, we recruited 86 patients who were suspected of PJI after total joint replacement (TJA) and examined their synovial fluid using CLIA to explore the clinical application value of these methods and the diagnostic efficiency of synovial fluid LTF and S100A8 for PJI. RESULTS: Our established CLIA methods have a wide linear range of 20-10,000 ng/mL for LTF detection system and 5-5000 ng/mL for S100A8 detection system. Performance parameters such as precision, specificity, and recovery rate can meet the industry standards. Then, the established methods were used to detect LTF and S100A8 in synovial fluid samples, which showed excellent diagnostic efficiency for PJI, and the areas under ROC curve (AUC) were 0.954 (95 % CI: 0.909-0.999) and 0.958 (95 % CI: 0.918-0.997), respectively. CONCLUSION: Our established CLIA methods have the advantages of automation, high throughput, low price, and is expected to be widely popularized in clinical applications. Synovial fluid LTF and S100A8 detected through CLIA had efficient diagnostic potentiality for predicting and diagnosing PJI.

A Cobalt-Based Metal-Organic Framework Nanosheet as the Electrode for High-Performance Asymmetric Supercapacitor.

Inspired by the significant advantages of the bottom-up synthesis whose structures and functionalities can be customized by the selection of molecular components, a 2D metal-organic framework (MOF) nanosheet Co-BTB-LB has been synthesized by a liquid-liquid interface-assisted method. The as-prepared Co-BTB-LB is identified by scanning electron microscopy/energy dispersive spectroscopy (SEM/EDX) and X-ray photoelectron spectroscopy (XPS), and the sheet-like structure is verified by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), and atomic force microscopy (AFM). Co-BTB-LB electrode exhibits an excellent capacity of 4969.3 F g-1 at 1 A g-1 and good cycling stability with 75% capacity retention after 1000 cycles. The asymmetric supercapacitor device with Co-BTB-LB as the positive electrode shows a maximum energy density of 150.2 Wh kg-1 at a power density of 1619.2 W kg-1 and good cycling stability with a capacitance retention of 97.1% after 10000 cycles. This represents a state-of-the-art performance reported for asymmetric supercapacitor device using electroactive bottom-up metal-complex nanosheet, which will clearly lead to a significant expansion of the applicability of this type of 2D nanomaterials.

Synthesis and chiral recognition properties of novel fluorescent chemosensors for amino acid.

The charge neutral chiral optical sensors 1a-d containing thiourea and amide groups were synthesized by simple steps in good yields and their structures were characterized by IR, 1H NMR, 13C NMR, MS spectra and elemental analysis. The enantioselective recognition for alpha-phenylglycine and phenylglycinol was examined by fluorescence emission and UV-vis spectra. The fluorescence and UV-vis spectra changes of 1a were obvious when the enantiomers of alpha-phenylglycine anion were added, which exhibited that 1a has good enantioselective recognition ability towards alpha-phenylglycine.