Unconventional posttranslational modification in innate immunity.

Pattern recognition receptors (PRRs) play a crucial role in innate immunity, and a complex network tightly controls their signaling cascades to maintain immune homeostasis. Within the modification network, posttranslational modifications (PTMs) are at the core of signaling cascades. Conventional PTMs, which include phosphorylation and ubiquitination, have been extensively studied. The regulatory role of unconventional PTMs, involving unanchored ubiquitination, ISGylation, SUMOylation, NEDDylation, methylation, acetylation, palmitoylation, glycosylation, and myristylation, in the modulation of innate immune signaling pathways has been increasingly investigated. This comprehensive review delves into the emerging field of unconventional PTMs and highlights their pivotal role in innate immunity.

Multistate Reaction Coordinate Model for Charge and Energy Transfer Dynamics in the Condensed Phase.

Constructing multistate model Hamiltonians from all-atom electronic structure calculations and molecular dynamics simulations is crucial for understanding charge and energy transfer dynamics in complex condensed phases. The most popular two-level system model is the spin-boson Hamiltonian, where the nuclear degrees of freedom are represented as shifted normal modes. Recently, we proposed the general multistate nontrivial extension of the spin-boson model, i.e., the multistate harmonic (MSH) model, which is constructed by extending the spatial dimensions of each nuclear mode so as to satisfy the all-atom reorganization energy restrictions for all pairs of electronic states. In this work, we propose the multistate reaction coordinate (MRC) model with a primary reaction coordinate and secondary bath modes as in the Caldeira-Leggett form but in extended spatial dimensions. The MRC model is proven to be equivalent to the MSH model and offers an intuitive physical picture of the nuclear-electronic feedback in nonadiabatic processes such as the inherent trajectory of the reaction coordinate. The reaction coordinate is represented in extended dimensions, carrying the entire reorganization energies and bilinearly coupled to the secondary bath modes. We demonstrate the MRC model construction for photoinduced charge transfer in an organic photovoltaic caroteniod-porphyrin-C60 molecular triad dissolved in tetrahydrofuran as well as excitation energy transfer in a photosynthetic light-harvesting Fenna-Matthews-Olson complex. The MRC model provides an effective and robust platform for investigating quantum dissipative dynamics in complex condensed-phase systems since it allows a consistent description of realistic spectral density, state-dependent system-bath couplings, and heterogeneous environments due to static disorder in reorganization energies.

Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice.

BACKGROUND: As global warming becomes increasingly severe, it is urgent that we enhance the heat tolerance of crops. We previously reported that Arabidopsis thaliana PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C9 (AtPLC9) promotes heat tolerance. RESULTS: In this study, we ectopically expressed AtPLC9 in rice to examine its potential to improve heat tolerance in this important crop. Whereas AtPLC9 did not improve rice tolerance to salt, drought or cold, transgenic rice did exhibit greater heat tolerance than the wild type. High-throughput RNA-seq revealed extensive and dynamic transcriptome reprofiling in transgenic plants after heat stress. Moreover, the expression of some transcription factors and calcium ion-related genes showed specific upregulation in transgenic rice after heat stress, which might contribute to the enhanced heat tolerance. CONCLUSIONS: This study provides preliminary guidance for using AtPLC9 to improve heat tolerance in cereal crops and, more broadly, highlights that heterologous transformation can assist with molecular breeding.

[Trigger effect of hMG and hCG in the treatment of unexplainable non-obstructive azoospermia].

OBJECTIVE: To investigate whether the trigger effect of human menopausal gonadotropins (hMG) and human chorionic gonadotropins (hCG) attributes to the treatment of unexplainable non-obstructive azoospermia (NOA). METHODS: We retrospectively analyzed the clinical data about 282 cases of unexplainable NOA treated in the Maternity and Child Health Hospital of Guizhou Province from January 2010 to May 2017. All the patients underwent trigger treatment by intramuscular injection of hMG at 75 IU 3 times a week for 2 weeks, followed by hCG at 2 000 IU twice a week for another 2 weeks, and meanwhile took vitamin E, Levocarnitine and Tamoxifen as an adjunctive therapy. The treatment lasted 3－12 months. RESULTS: Fifty-eight of the 255 patients that completed the treatment were found with sperm in the semen after treatment, all with severe oligoasthenospermia. Forty-seven of the 58 cases received assisted reproductive technology (ART), of which 18 achieved clinical pregnancy. Semen centrifugation revealed no sperm in the other cases, of which 6 were found with epididymal sperm at epididymal and testicular biopsy after treatment and 3 of them achieved clinical pregnancy after ART. Sperm was found in the semen or at epididymal or testicular biopsy in 64 of the patients after treatment, with an effectiveness rate of 25.1%. CONCLUSIONS: Trigger treatment by injection of hMG and hCG combined with adjunctive oral medication has a certain effect on unexplainable NOA.