RESUMEN
Bowen's disease (BD) is a relatively rare early-stage squamous cell carcinoma in situ, most commonly affecting the middle-aged and elderly, and occurring on the skin or mucous membranes of various parts of the body. Its onset is concealed, the course of the disease is chronic, and some patients have malignant tumors outside the skin; therefore, it is necessary to diagnose and evaluate the disease at an early stage. This study aimed to investigate the application of reflectance confocal microscopy (RCM) in the diagnosis of BD. We performed RCM imaging on the lesion site and underwent skin biopsy for histological diagnosis of 92 patients initially diagnosed with BD in clinic. A retrospective analysis of the RCM result as well as the histological examination revealed that after analyzing RCM images, out of 92 biopsy lesions, 61 were diagnosed with BD, of which 54 were consistent with RCM diagnosis. Among the 59 cases diagnosed with BD by RCM, 54 cases were consistent with the histological diagnosis. Afterwards, we analyzed the RCM characteristics in patients with BD verified by biopsy, and compared the RCM images of two different lesions, classic Bowen's disease and pigmented Bowen's disease, and further summarized the key points of BD under RCM. Finally, we focused on the differential characteristics between BD and other skin diseases in RCM. RCM is of great value in the diagnosis of BD. RESEARCH HIGHLIGHTS: A retrospective study of RCM and histological diagnosis in patients with clinical diagnosis of BD. Analyze the RCM characteristics of skin lesions verified by biopsy. RCM is of great value in the diagnosis and differentiation of BD.
RESUMEN
The key of phytoremediation was researches and selecting of dominant species in the lead-zinc tailings. This assay analyzed the amount of heavy metals, and the enrichment and transporting features of heavy metals, such as Pb, Zn, Cu, Cd and Mn, in the dominant species in the district, and then we can select the pioneer plants for ecosystem restoration of the area. Results showed that there were 40 species of higher plants, which belonged to 40 chasses and 22 families in the area. And we selected 15 dominant species by heavy metals. Among these dominant species, the content of Pb in Ficus tikoua was 4. 01 times higher than that in other plants, the transfer factor (TF) was 3.91, and bioaccumulation factor (BCF) was 14.4. The capability of TF and BCF of Ficus zikoua was high, so Ficus tikoua had potentials in phytoremediation of heavy metals in polluted area and its enrichment capability of Pb was worth for the further research. Apart from Ficus zikoua, other 14 kinds of dominant plants had a better tolerance in metal pollution in tailings, and they can be used as pioneer plants of ecological rehabilitation in lead-zinc tailings in Xiashuiwan.
