A new core-shell-type nanoparticle loaded with paclitaxel/norcantharidin and modified with APRPG enhances anti-tumor effects in hepatocellular carcinoma.

Nanoparticle delivery systems have been shown to improve the therapeutic efficacy of anti-cancer drugs, including a variety of drugs for the treatment of hepatocellular carcinoma (HCC). However, the current systems show some limitations, and the delivery of more effective nanoparticle systems for anti-HCC drugs with better targeting ability are needed. Here, we created paclitaxel (PTX)/norcantharidin (NCTD)-loaded core-shell lipid nanoparticles modified with a tumor neovasculature-targeted peptide (Ala-Pro-Arg-Pro-Gly, APRPG) and investigated their anti-tumor effects in HCC. Core-shell-type lipid nanoparticles (PTX/NCTD-APRPG-NPs) were established by combining poly(lactic-co-glycolic acid) (PLGA)-wrapped PTX with phospholipid-wrapped NCTD, followed by modification with APRPG. For comparison, PTX-loaded PLGA nanoparticles (PTX-NPs) and PTX/NCTD-loaded core-shell-type nanoparticles without APRPG (PTX/NCTD-NPs) were prepared. The in vitro and in vivo anti-tumor effects were examined in HepG2 cells and tumor-bearing mice, respectively. Morphological and release characterization showed that PTX/NCTD-APRPG-NPs were prepared successfully and achieved up to 90% release of PTX in a sustained manner. Compared with PTX/NCTD-NPs, PTX/NCTD-APRPG-NPs significantly enhanced the uptake of PTX. Notably, the inhibition of proliferation and migration of hepatoma cells was significantly higher in the PTX/NCTD-APRPG-NP group than those in the PTX-NP and PTX/NCTD-NP groups, which reflected significantly greater anti-tumor properties as well. Furthermore, key molecules in cell proliferation and apoptosis signaling pathways were altered most in the PTX/NCTD-APRPG-NP group, compared with the PTX-NP and PTX/NCTD-NP groups. Collectively, PTX/NCTD-loaded core-shell lipid nanoparticles modified with APRPG enhance the effectiveness of anti-HCC drugs and may be an effective system for the delivery of anti-HCC drugs.

Study on the quality standard of improvement of Tongluo-Zhujing pills / 国际中医中药杂志

Objective To improve the quality standard of Tongluo-Zhujing pills.Methods We added the identification ofradix notoginseng andtypha orientalisby TLC and determination of the content of wedelolactone by HPLC. The study used Agilent ZORBAX SB-C18 (4.6 mm × 250 mm, 5 μm) column and the velocity of flow manufactured by the proper proportion was 1 ml/min. The detection wavelength was 249 nm . Results The new method could well identify radix notoginsengandtypha orientalis. The calibration curves of wedelolactone was in good linearity over the range of 0.064-0.642 μg (r=0.999 7) and the average recovery was 98.74%(RSD=1.61%).Conclusions The quality standard of Tongluo-Zhujing pills was proved effectively.

Foundation support for studies on single-cell sequencing and its application in USA, Britain and China / 中华医学图书情报杂志

Objective To study the distribution and development of single-cell sequencing in China from the govern-mental foundation support and the gap with the developed countries in order to provide reference for the relevant de-cision-making departments in promoting further studies on single-cell sequencing. Methods The information was accessed from the foundation support platforms in USA, Britain and China, and the foundation support for studies on single-cell sequencing in USA, Britain and China was analyzed. Results The USA and Britain supported 540 million dollars and 40 million pounds respectively for studies on single-cell sequencing, China supported 100 mil-lion RMB for studies on microbiology, oncology and reproductive medicine with stress laid on technology develop-ment. Conclusion The starting time of foundation support for studies on single-cell sequencing is later in China than in USA and Britain, it is thus necessary for China to promote the application of single-cell sequencing technology by making full use of the existing resources.

Foundation support for studies on single-cell sequencing and its application in USA, Britain and China / 中华医学图书情报杂志

Objective To study the distribution and development of single-cell sequencing in China from the govern-mental foundation support and the gap with the developed countries in order to provide reference for the relevant de-cision-making departments in promoting further studies on single-cell sequencing. Methods The information was accessed from the foundation support platforms in USA, Britain and China, and the foundation support for studies on single-cell sequencing in USA, Britain and China was analyzed. Results The USA and Britain supported 540 million dollars and 40 million pounds respectively for studies on single-cell sequencing, China supported 100 mil-lion RMB for studies on microbiology, oncology and reproductive medicine with stress laid on technology develop-ment. Conclusion The starting time of foundation support for studies on single-cell sequencing is later in China than in USA and Britain, it is thus necessary for China to promote the application of single-cell sequencing technology by making full use of the existing resources.

Effects of Xuesaitong on the Retinal Microcirculation of Patients with Diabetic Retinopathy / 现代生物医学进展

Objective:To investigate the effects of xuesaitong on the retinal microcirculation of patients with diabetic mtinopathy (DR).Methods:Ninety-five patients with DR admitted in our hospital were randomly divided into the control group and the observation group.Forty-eight patients in the control group were treated with conventional hypoglycemic agent,and those in the observation group were treated with xuesaitong.The retinal microcirculation indexes including EDV,PSV,RI,Vmax,Vmin,MV,hemorheology indexes including NBL,NBH,DE,Hct,AE,ESR,and clinical efficacy in both groups were observed and compared.Results:After treatment,the clinical efficacy of observation group was 87.8％,which was much higher than that of the control group (61.4％,P＜0.05).The EDV,Vmax,and Vmin of control group were significantly improved than those before treatment (P＜0.05),and EDV and PSV were much higher than those of control group,the RI,Vmax,Vmin,and MV were much lower (P＜0.05).Additionally,the NBL and NBH in control group were much lower compared with those before treatment,while NBL,NBH,DE,Hct,AE,and ESR in control group were improved than those in control group,which were much improved that those of control group (P＜0.05).Conclusion:Xuesaitong combined with conventional hypoglycemic therapy was effective in treating patients with diabetic retinopathy,which could significantly improved the retinal microcirculation and hemorheology.

Research progress of bronchoalveolar lavage in rats and mice / 中国比较医学杂志

Bronchoalveolar lavage is an important animal experimental technique in the study of respiratory system and its pathological changes. It can acquire a variety of biochemical factors, inflammatory mediators and immune cells from the respiratory tract and lungs, and provides an important evaluation index and reference for animal experiment. Bronchoalveolar lavage is an effective and reliable method for the diagnosis of respiratory diseases. It has been gradually standardized and widely used in clinical practice at present, however, there is no set of standard for bronchoalveolar lavage in rats and mice. The results of bronchoalveolar lavage fluid are affected by many factors, such as the lavage fluid, suction pressure, the amount of lavage and recovery, and the retention time of lavage fluid in the lungs. Successful and efficient acquisition of lavage specimens is the key to the study and evaluation of respiratory diseases. This paper summarizes the current lavage methods commonly used by domestic and foreign researchers, and provides a reference for further research in the this field.

Identification of predominant prevailing nontuberculosis mycobacteria in Hangzhou and analysis of their predisposing factors and drug resistance / 中国人兽共患病学报

This study is aim to determine the predominant prevailing nontuberculosis mycobacteria (NTM) in Hangzhou and to understand the predisposing factors and drug resistance of NTM.PNB/TCH growth tests were used to preliminarily identify the NTM in the mycobacterium-positive culture samples from 1 972 patients.The species of NTM isolates were con firmed using mycobacterium molecular linear probe method and sequencing of PCR product of 16S rRNA gene.The influence of sex and age on predisposing factors of NTM was subsequently analyzed.Concentration proportional method was applied to de tect the susceptibility of NTM isolates to armazide,rifampicin,streptomycin,ethambutol,ofloxacin and kanamycin.Results of PNB/TCH growth tests showed that 9.8％ (193/1 972) of the 1 972 mycobacterium-positive culture samples were positive.The mycobacterium molecular linear probe hybridization and PCR product sequencing confirmed that in the 193 samples with positive results of PNB/TCH growth tests,66.3％,18.1％,8.3％ and 7.3％ were infected with NTM alone,two mycobacteria,Mycobacterium tuberculosis and bacteria that not belonging to the genus of Mycobacteria,respectively.In the 173 NTM isolates (128 with single infection and 45 with mixed infection),57.8％ were identified as Mycobacterium intracellulare,followed by Mycobacterium abscessus (12.1％),Mycobacterium kansasi i (9.8％),Mycobacterium chelonei (9.8％) and Mycobacterium avium (5.8％).In the 35 mixed infection samples,28.5％ and 20.0％ were the co-infection of M.intracellulare and M.chelonei with M.tuberculosis,respectively.Male patients infected with NTM were more than female patients (1.67 ∶ 1) and the infection rate (80.4％) of populations with age over 50 years old was significantly high than that with lower than 50 years old (P＜0.01).Pulmonary infection proportion (95.1 ％) of NTM was significantly higher than other positions of body (P＜0.01).100％ of NTM isolates were resistant to armazide while the resistance rates of the isolates against the other 5 antituberculosis drugs were as high as 70.3 ％ 90.6 ％.As the conclusion of this study,M.intracellulare is the predominant prevailing NTM species in the recent years in Hangzhou,NTM mainly causes pulmonary infection and middle-aged and old people are easily infected by NTM.Moreover,NTM isolates have high resistance against commonly used antituberculosis drugs.

Neurons in the hippocampus of chemobrain versus non-chemotherapy brain / 中国组织工程研究

BACKGROUND:Chemotherapy drugs have been shown to penetrate the blood-brain barrier and cause neurotoxicity,further damaging nerve cells.OBEJCTIVE:To investigate the changes in the expression and morphology of hippocampal neurons in chemobrain,and to investigate the underlying mechanism of nerve regeneration in chemobrain.METHODS:The brain specimens were obtained from the six patients undergoing cancer chemotherapy and six patients without chemotherapy,and were then subdivided into two groups:＜ 60 years and ＞ 60 years groups (n=3 per group).Immunofluorescence staining with specific antibodies was used to detect the changes in the morphology and number of neurons in the hippocampus CA1,CA2 and CA3.RESULTS AND CONCLUSION:The number of neurons positive for NeuN,DCX and PV in the hippocampal CA1,CA2 and CA3 in the chemotherapy group was significantly lower than that in the non-chemotherapy group.Moreover,the neurons positive for PV were smaller,with less and shorter nervous processes.Immunofiuorescence staining findings showed that the ＜ 60 years old patients in the chemotherapy group had less neurons positive for NeuN and DCX than that in the non-chemotherapy;for ＞ 60 years old patients,there was no significant difference between two groups.These findings suggest that chemotherapy can downregulate the expression of immature neurons in the hippocampus and reduce the number of neurons in the hippocampus.Moreover,chemotherapy can change the morphology and reduce the number of PV neurons.

Spatial Analysis on Primary Liver Cancer Incidence in Sihui County,Guangdong Province, 2004-2011 / 中山大学学报(医学科学版)

[Objective]To explore the spatial distribution pattern of liver cancer in Sihui city ,Guangdong Province from 2004 to 2011,and to provide theoretical evidence for prevention and control of liver cancer.[Methods]Spatial scan statistic was applied,and analysis were conducted by using software SaTScan 9.4.4 to detect the clusters in Sihui from 2004 to 2011.Map making and results visualizing were carried out with software ArcGIS 10.2.[Results]A total of 1252 cases was reported in Sihui City,Guangdong Province from 2004 to 2011,with the incidence of liver cancer increasing from 32.67/100000 to 41.14/100000. Spatial scans results showed that highly clustered areas are mainly located in the south and southeast,which include Dasha,Longfu and Dongcheng countries in all people (LLR=6.34,P=0.019,RR=1.31) and the male (LLR=5.83,P=0.025,RR=1.33),respectively;highly clustered areas in female were include Dasha,Dongcheng,Chengzhong and other two countries(LLR=5.18,P=0.043,RR=1.41). Lowly clustered areas in all people are mainly located in the north,which include Luoyuan,Didou and Jiangfu countries(LLR=8.66, P=0.001,RR=0.67).[Conclusion]The incidence of primary liver cancer in Sihui was increasing over time,and spatial clustering of the cases were observed. Further study should be focused on the risk factors of liver cancer in related areas to strengthen the prevention and control of liver cancer.

Co-expression of GRP78 and Myocilin in trabecular meshwork cells of primary open angle glaucoma / 中华实验眼科杂志

Background Misfolding of Myocilin protein plays an important role in the pathogenesis of primary open angle glaucoma (POAG).GRP78 can transfer the misfolded proteins out endocytoplasmic reticulum and keep the protein synthesis function of endoplasmic reticulum.However the relationship between GRP78 and Myocilin in the pathogenesis of POAG has not been elucidated.Objective This study was to investigate and analyze the coexpressions of GRP78 and Myolicin in trabecular meshwork cells (TMCs) of POAG.Methods The trabecular meshwork tissues were obtained during the surgery of POAG and healthy donor for the isolated and in vitro culture of TMCs,and the morphology of the cells was compared between POAG-derived TMCs and donor-derived TMCs.The expression of specific factors for TMCs were detected by immunochemistry.The cells were divided into normal control group,tunicamycin (Tm)-treated group and staurosporine (STS)-treated group,and the cells were cultured by regular medium,the 1 μ mol/L Tm medium or 0.1 μmol/L STS medium for 24 hours respectively.The co-expression of GRP78 and Myocilin in the cells were detected using immunofluorescence assay.This study was approved by Ethics Committee of Xi'an No.4 Hospital,and written informed consent was obtained from each patient before surgery.Results Primarily cultured cells showed the fiat star-like and irregular appearance with 3-5 processes,round nuclei,abundant cytoplasm and black engulf particles.The 3-7 generations of POAG-derived cells grew well,showing positive expressions of laminin (LN),fibronectin (FN),neuronal specific enolase (NSE) and Vimentin.Immunofluorescence assay showed positive expressions of GRP78 and Myocilin in the cells of the Tm group,STS group and normal control group,with the reddish and green fluorescence separately.Incomplete co-expression of GRP78 and Myocilin was seen in donor-derived TMCs,and complete co-expression of GRP78 and Myocilin was found in the POAG-derived TMCs in the normal control group.Complete co-expression of GRP78 and Myocilin was exhibited in both donor-and POAG-derived TMCs in the Tm group and STS group.In addition,accumulation of GRP78 protein around nucleus was seen in both donor-and POAG-derived TMCs in the Tm group.Conclusions GRP78 and Myocilin proteins are completely co-expressed in POAG-derived TMCs,inferring that GRP78 and Myocilin play an interaction role in the pathogenesis and development of POAG.GRP78 may play a cytoprotective role against the apoptosis of TMCs caused by endoplasmic reticulum stress.

Registration of 30 026 cases of Shenqi Fuzheng injection combined with other drugs in real world / 中国中药杂志

Prospective, multi-center, large-sample and registered design was used to analyze the drug combination features of Shenqi Fuzheng injection in the real world clinical application, and comprehend the drug combination in the real world. A total of 30 026 patients with the use of Shenqi Fuzheng injection were registered, where the chemical drugs were used for 57 436 times (accounting for 82.76%), and the Chinese patent medicines were used for 11 962 times (accounting for 17.24%), mainly including anti-acid drugs and anti-ulcer drugs, nutritional agent, immune enhancement agent, etc. According to the association rules, drug combinations of 2 drugs were closely related to inhibiting gastric acid secretion and anti-tumor; drug combinations of 3 drugs were closely related to inhibiting gastric acid secretion, antiemetic and anti tumor; drug combinations of 4 drugs were closely related to inhibiting gastric acid secretion, antiemetic, anti-tumor, and immune enhancement. The above results were consistent with the Instruction, providing clues for accurate treatment, and laying the foundation for clinical rational drug use.

Analysis of the allergic reaction types of Chinese medicine injection based on immunotoxicty / 中国中药杂志

The purpose of the study is to find the allergic reaction types and characteristics of Chinese medicine injection ( CMI). The authors monitored patients who used Shuxuetong injection, Dengzhan Xixin injection, Shenqi Fuzheng injection, Shenmai injection, Ciwujia injection, Shuxuening injection, Tanreqing injection, Reduning injection, a total of 150,000 cases were monitored. They used a nested case-control design to group the patients and obtained the serum samples from 14 allergic patients and 55 matched patients. They used enzyme-linked immunosorbent assay (ELISA) to detect serum C3, C4, IgE, IgG, MCT-P, and judge the allergic reaction types: Shuxuetong injection hypersensitivity (1 case), can not determine (1 case); Dengzhan Xixin injection hypersensitivity (1 case), hypersensitivity & anaphylactoid reaction (1 case), can not determine (1 case); Shenqi Fuzheng injection hypersensitivity (3 cases), can not determine (1 case); Shenmai injection anaphylactoid reaction (1 case); Ciwujia injection can not determine (1 case), Shuxuening injection can not determine (1 case), Tanreqing injection can not determine (1 case), Reduning injection can not determine (1 case). The results showed that the main type of allergic reaction of CMI was hypersensitivity, the type of allergic reaction was closely related to the varieties of CMI, the hypersensitivity and anaphylactoid reaction might occur in one patient at the same time which used Dengzhan Xixin injection.

Post-marketing clinical safety assessment of Shenmai injection based on active monitoring and passive monitoring in large data background / 中国中药杂志

This paper adopted a series of related analysis methods to comprehensively analyze post-marketing clinical safety data of Shenmai injection from 4,220 cases of SRS and 32,358 cases of multicenter, prospective, registered hospital centralized monitoring in large data background, calculated ADR incidence rate was 0.93 per 1,000, main symptoms of ADR includes chest pain, chills, skin itching, palpitations, fever, nausea, dizziness, vomiting, flushing, numbness, allergic reaction, cyanosis, rash, low back pain, and "breath", "anaphylactoid reaction" and "flush" were the safety warning signals of Shenmai injection. Primary disease for chronic pulmonary heart disease, thyroid disease, and combined with cerebral vascular disease, prior to the injection and continuous use of alprostadil, cyclic adenosine monophosphate, combined with quinolones, penicillins were suspicious influence factors of ADR of Shenmai injection, these promot the clinical safety.

Clinical safety studies based on 30 026 post-marketing cases of Shenqi Fuzheng injection by intensive hospital monitoring nested NCCS / 中国中药杂志

This study adopted a large sample, multicenter, registered hospital centralized monitoring nested prospective case-control study design. From the real world clinical application of Shenqi Fuzheng injection, monitored 30 026 cases of patients with the use of Shenqi Fuzheng injection. A total of 51 adverse drug reaction (ADR) cases was monitored, including 1 case of severe adverse reactions. ADR incidence rate was 1.7 per 1,000. Blood samples were collected from patients with allergic reactions and their matched controls. Related biological indicators of allergic reactions were unified detected and analysed in order to explore the mechanism of allergic reaction and promote the clinical safety.

Activation of γ-aminobutyric Acid (A) Receptor Protects Hippocampus from Intense Exercise-induced Synapses Damage and Apoptosis in Rats / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Our previous study has confirmed that one bout of exhaustion (Ex) can cause hippocampus neurocyte damage, excessive apoptosis, and dysfunction. Its initial reason is intracellular calcium overload in hippocampus triggered by N-methyl-D-aspartic acid receptor (NMDAR) over-activation. NMDAR activation can be suppressed by γ-aminobutyric acid (A) receptor (GABAAR). Whether GABAAR can prevent intense exercise-induced hippocampus apoptosis, damage, or dysfunction will be studied in this study.</p><p><b>METHODS</b>According to dose test, rats were randomly divided into control (Con), Ex, muscimol (MUS, 0.1 mg/kg) and bicuculline (BIC, 0.5 mg/kg) groups, then all rats underwent once swimming Ex except ones in Con group only underwent training. Intracellular free calcium concentration ([Ca2+]i) was measured by Fura-2-acetoxymethyl ester; glial librillary acidic protein (GFAP) and synaptophysin (SYP) immunofluorescence were also performed; apoptosis were displayed by dUTP nick end labeling (TUNEL) stain; endoplasmic reticulum stress-induced apoptosis pathway was detected by Western blotting analysis; Morris water maze was used to detect learning ability and spatial memory.</p><p><b>RESULTS</b>The appropriate dose was 0.1 mg/kg for MUS and 0.5 mg/kg for BIC. Ex group showed significantly increased [Ca2+]i and astrogliosis; TUNEL positive cells and levels of GFAP, B cell lymphoma-2 (Bcl-2) associated X protein (Bax), caspase-3, caspase-12 cleavage, CCAAT/enhancer binding protein homologous protein (CHOP), and p-Jun amino-terminal kinase (p-JNK) in Ex group also raised significantly compared to Con group, while SYP, synapse plasticity, and Bcl-2 levels in Ex group were significantly lower than those in Con group. These indexes were back to normal in MUS group. BIC group had the highest levels of [Ca2+]i, astrogliosis, TUNEL positive cell, GFAP, Bax, caspase-3, caspase-12 cleavage, CHOP, and p-JNK, it also gained the lowest SYP, synapse plasticity, and Bcl-2 levels among all groups. Water maze test showed that Ex group had longer escape latency (EL) and less quadrant dwell time than Con group; all indexes between MUS and Con groups had no significant differences; BIC had the longest EL and least quadrant dwell time among all groups.</p><p><b>CONCLUSIONS</b>Activation of GABAA R could prevent intense exercise-induced synapses damage, excessive apoptosis, and dysfunction of hippocampus.</p>

Digit and digit ratio of Xinjiang young Kazakh nationality / 中国组织工程研究

BACKGROUND:Digit ratio is basical y determined within the first 3 months of fetal development. Digit and digit ratio exhibit differences between genders and between left and right hands. Nationality, regional and ethnic difference may also influence the digit ratio. So far, there is no report about the digit ratio of Kazakh adults. OBJECTIVE:To study the characteristics of digit ratio of young Kazakh nationality in Xinjiang of China. METHODS:A total of 200 young Kazakh volunteers in Xinjiang, aged 18-25 years, were enrol ed, including 100 males and 100 females. These participants were subjected to posteroanterior X-ray of both hands. Phalanx length and body height were measured, and the resulting data were statistical y analyzed using SPSS19.0 software. RESULTS AND CONCLUSION:The digit ratio of Xinjiang Kazakh people was 3D>4D>2D>5D. Male digit ratio possessed the tendency of 3D:5D>4D:5D>2D:5D>3D:4D>3D:4D>2D:3D>2D:4D;female digit ratio possessed the tendency of 3D:5D>2D:5D>3D:4D>4D:5D>2D:4D>2D:3D. The digit ratio of 2D:3D, 3D:4D, 4D:5D was higher in the male than the female;the digit ratio of 2D:4D, 2D:5D, 3D:5D was higher in the female than the male. Digit ratio had sexual differences, especial y in 3D:5D (P<0.05), and had bilateral differences, especial y in 2D:4D and 4D:5D (P<0.05). These findings indicate that the digit ratio of Xinjiang Kazakh people exhibits a bilateral difference, especial y in 2D:4D and 4D:5D, as wel as a sexual difference, especial y in 3D:5D .

Intense exercise can cause excessive apoptosis and synapse plasticity damage in rat hippocampus through Ca²⁺ overload and endoplasmic reticulum stress-induced apoptosis pathway / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Intense exercise can cause injury and apoptosis, but few studies have reported its effect on the central nervous system (CNS). The initial reason for hippocampus injury is the excitotoxicity of glutamate and calcium overload. Intracellular free Ca(2+) ([Ca(2+)]i) overload may trigger the apoptosis pathway and neuron damage. The aim of this study was to investigate whether intense exercise could cause hippocampus apoptosis and neuron damage and then to determine which pathway was activated by this apoptosis.</p><p><b>METHODS</b>We used one bout of swimming exhaustion rats as models. Intracellular [Ca(2+)]i was measured to estimate the calcium overload by Fura-2/AM immediately after exhaustion; glial fibrillary acidic protein (GFAP) and synaptophysin (SYP) immunofluorescence were performed for estimating astrocyte activation and synapse plasticity 24 hours after exhaustion. Apoptosis cells were displayed using dUTP nick end labelling (TUNEL) stain; endoplasmic reticulum (ER) stress-induced apoptosis pathway and mitochondrial apoptosis pathway were synchronously detected by Western blotting.</p><p><b>RESULTS</b>An increasing level of intracellular [Ca(2+)]i (P < 0.01) was found in the hippocampus immediately after exhaustion. GFAP and SYP immunofluorescence showed that the astrocytes are activated, and the synapse plasticity collapsed significantly 24 hours after exhaustion. TUNEL stain showed that the number of apoptosis cells were notably raised (P < 0.01); Western blotting of the apoptosis pathway showed increasing levels of caspase-3 cleavage (P < 0.01), Bax (P < 0.01), caspase-12 cleavage (P < 0.01), C/EBP-homologous protein (CHOP) (P < 0.01), and phospho-Junaminoterminal kinases (p-JNK; P < 0.01) and decreasing level of Bcl-2 (P < 0.01). Our results proved that exhaustion can induce hippocampus injury and apoptosis by [Ca(2+)]i overload, with collapsed synaptic plasticity as the injury pattern and ER stress-induced apoptosis as the activated pathway.</p><p><b>CONCLUSION</b>Intense exercise can cause excessive apoptosis and synapse plasticity damage in the hippocampus with [Ca(2+)]i overload as the initial reason, and thus provides leads for therapeutic interventions in the brain health of athletes.</p>

Study of post marketing safety reevaluation of shenqi fuzheng injection / 中国中药杂志

In order to promote the Shenqifuzheng injection (SQFZ) clinical medication safety, this study reevaluate on SQFZ post marketing safety study systematically. Including multi center large sample registration type safety monitoring research, the analysis based on national spontaneous reporting system data, the analysis based on the 20 national hospital information system data and literature research. Above the analysis, it suggests that SQFZ has good security. The more adverse drug reaction (ADR) as allergic reactions, mainly involved in the damage of skin, appendages and its systemic damage, serious person can appear allergic shock. ADR/E is more common in the elderly, may be related to medication (tumor) populations. Early warning analysis based on SRS data and literature research are of the view that "phlebitis" has a strong association with SQFZ used.

Impact analysis of shenqi fuzheng injection of course on liver function based on hospital information system data / 中国中药杂志

<p><b>OBJECTIVE</b>Use propensity score methods to explore the effect of Shenqi Fuzheng injection (SQFZ) on ALT and AST lever of real worlds.</p><p><b>METHOD</b>HIS data from 18 hospitals was analyzed. Patients ranging from 18 to 80 years, it were divided into the cases group was used Shenqi Fuzheng injection > 21 days under the instruction, and control group used SQFZ ≤ 21 days. A large number of confounding biases are taken into account through the generalized boosted models (GBM) and multiple logistic regression model to estimate the treatment effects of SQFZ on abnormal changes in ALT and AST index and to explore possible influencing factors.</p><p><b>RESULT</b>Sventy and one confounding factors had been counterpoised, stratified analysis showed that the abnormal changes of cases group lower than control group, but not statistically significant.</p><p><b>CONCLUSION</b>Analysis of HIS database showed that significant effects of SQFZ on abnormal changes in ALT and AST have not been found. The results of this study form the retrospective data, for the purpose of validating its clinical safety should be have a prospective clinical study of large sample.</p>

Analysis of kudiezi injection different dosage impact on patient's liver and kidney function based on hospital information system / 中国中药杂志

This study aims to explore the impact on patient's liver and kidney function by different dosage of Kudiezi injection. This study retrospectively analyzed 15 228 patients' records from 18 nationwide general hospital information system (HIS). All patients were treated with Kudiezi injection, 1 956 patients that were given doses of > 40 mL, which is above the recommended dose, acted as the observation group. Fifty-five patients receiving the recommended dose of < 40 mL were the control group. Data about alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr) and blood urea nitrogen (BUN) were collected before and after using Kudiezi injection, changes after treatment were outcomes. Also recorded were: age, costs, length of hospitalization and the patients' condition on admission. Propensity score method was used to balance 71 confounding variables such as gender, age, mortality, and costs. There were no significant difference on the four indexes between the two groups. It is hard to conclude that the use of Kudiezi injection over the recommended dose could influence the four indexes of liver and kidney from this data analysis. More conclusive evidence should be collected by further prospective study.