RESUMEN
Recent studies have revealed that the inflammatory process plays a role in the pathogenesis of osteoarthritis (OA). The S100 family and receptor for advanced glycation end products (RAGE) participate in regulating inflammation, even in the production of matrix metalloproteinases (MMPs). MMP-1 degrades cartilage, which may result in OA development. Moreover, polymorphisms in RAGE, S100A8, and MMP-1 have a marked effect on ligand binding and transcription regulating. In this study, we investigated the potential genetic contribution of the RAGE, S100A8, and MMP-1 genes to OA. We performed a matched case-control association study and genotyped OA patients and healthy controls, who were analyzed by polymerase chain reaction-restriction fragment length polymorphism assays. A total of 207 patients were diagnosed with knee OA and underwent total knee replacement. The control group included 207 individuals who had standard X-rays of the knee joints to confirm K/L < 2 and were matched by age and gender. Single-nucleotide polymorphisms in RAGE (-429T/C, -374T/A, and 557G/A), S100A8 (rs3795391A/G), and MMP-1 (-1607 1G/2G, -755G/T, and -519A/G) were evaluated. RAGE -374T/A, S100A8 rs3795391A/G, MMP-1 -1607 1G/2G, -755G/T, and -519A/G showed no significant difference between OA patients and healthy controls. RAGE -429T/C and 557G/A showed a significant association between OA patients and healthy controls (P = 0.016 and 0.047, respectively). In haplotype analyses, no RAGE and MMP-1 haplotypes showed associations with OA. Our results suggest that the investigated polymorphism in the RAGE gene play a role in OA in the Han Chinese population.
Asunto(s)
Pueblo Asiatico/genética , Etnicidad/genética , Predisposición Genética a la Enfermedad , Osteoartritis de la Rodilla/genética , Polimorfismo de Nucleótido Simple/genética , Receptor para Productos Finales de Glicación Avanzada/genética , Índice de Severidad de la Enfermedad , Anciano , Calgranulina A/genética , Estudios de Casos y Controles , Femenino , Estudios de Asociación Genética , Haplotipos/genética , Humanos , Masculino , Metaloproteinasa 1 de la Matriz/genéticaRESUMEN
The effect of tree diversity on productivity in subtropical forests in China is poorly understood. We investigated the biomasses of trees, understory vegetation, coarse roots, and fine roots with varying proportions of Pinus massoniana, mixed with other tree species in stands of the same age, to examine the effects of tree diversity. With an increase of P. massoniana proportion, the tree and understory biomasses increased at first, and then gradually decreased. As expected, the biomass of fine roots decreased with soil depth. Stands with 40 to 60% P. massoniana had the highest biomass, whereas stands with <20% P. massoniana had the least biomass. Stands with <20% P. massoniana had the least understory biomass, whereas those with 20 to 40% Masson pine had the least fine root biomass.