Rapid aerosol transmission of Salmonella among turkeys in a simulated holding-shed environment.

Once turkeys arrive at Midwest processing plants, they are usually held in large open-sided sheds for 1 to 4 h, waiting to be unloaded. In hot, humid weather, large fans are used to cool the birds. The resultant air currents distribute a significant amount of dust to the turkeys. The dust created in this environment could be a factor in the number of Salmonella-contaminated turkeys entering slaughter plants. The objective of this study was to determine if rapid transmission of Salmonella in turkeys could occur from exposure to Salmonella-contaminated dust similar to what may be experienced in holding sheds or in other high-dust environments prior to slaughter. In the first experiment, trials of 3 different concentrations of Salmonella (1.2 x 10(9), 2.6 x 10(7), and 2.6 x 10(5) cfu/g) were conducted to determine if transmission of Salmonella enterica serovar Typhimurium var. typhimurium(chi)4232 to turkeys 2 to 4 h after aerosol exposure to contaminated feces is possible. Results showed that turkeys became infected after 2 h of exposure to airborne-contaminated feces with a concentration level of 2.6 x 10(5) cfu of Salmonella Typhimurium/g. In the second experiment, consisting of 3 trials, 1 bank (5 cages wide and 3 cages high) of turkeys (n = 15 birds per trial) was exposed to another bank of cages of S. Typhimurium-inoculated (n = 15) birds for 2 to 4 h using a fan similar to the type in processing-plant cooling sheds. Results from this experiment demonstrated that birds could be contaminated with S. Typhimurium after 2 h of exposure. Results of both studies implicate contaminated dust as a route of rapid airborne transmission of Salmonella in turkeys. Processes that generate significant dust prior to slaughter should be regarded as critical control points for Salmonella.

Salmonella prevalence in market-age turkeys on-farm and at slaughter.

The objective of this study was to compare the prevalence of Salmonella in market-age turkeys on-farm and at slaughter (i.e., before and after feed withdrawal, catching, loading, transportation, and preslaughter holding). Thirty birds were randomly selected from each of 6 commercial turkey flocks scheduled to be loaded and shipped to the abattoir during the evening of the same day. Selected birds were euthanized on the farm, and the cloacal contents, large intestine, crop, ceca, liver and gallbladder, and spleen were aseptically collected. At the abattoir, 30 birds from the same flock were randomly selected from the slaughter line, and the crop, ceca, liver and gallbladder, and spleen were collected for subsequent culture at the laboratory. All flocks studied were positive for Salmonella at slaughter. No statistical difference was found between the overall prevalence on-farm and at slaughter. At both sampling points, the overall prevalence found was 33.3%. Diverging prevalence estimates were obtained based on the different sample types collected on-farm and at slaughter. In both cases, cecal content samples had the highest relative sensitivity (73.3% on-farm and 68.3% at slaughter). This study demonstrates that the preslaughter practices of feed withdrawal, catching, loading, transportation, and holding do not significantly alter the prevalence of Salmonella in market-age turkeys. Therefore, our results suggest that it may be possible to monitor the Salmonella status of turkey production farms based on samples collected at the abattoir.

Quantity and distribution of Salmonella recovered from three swine lairage pens.

The quantity of Salmonella recoverable from three lairage pens in a swine abattoir was determined. Using dry four-ply cotton gauze pads measuring 10 by 10 cm, 100 fecal slurry samples were collected from each of the three pens. Salmonella recovery was expressed as the log CFU per milliliter of sample. Mean values were 2.5 log CFU/ml in pen A, 2.7 log CFU/ ml in pen B, and 0.89 log CFU/ml in pen C. Median values were 2.6 log CFU/ml in pen A, 2.0 log CFU/ml in pen B, and below the detectable limit in pen C. In pen C, Salmonella was not recoverable from a high number of samples. Pen B results suggested spatial dependency, i.e., samples close together were more similar than samples farther apart. These results indicate that Salmonella concentrations vary within and between lairage pens. Because of the limited number of pens assessed, it was not possible to identify factors that were associated with the observed variation in Salmonella concentrations within and between pens. However, this variation suggests that numeroussamples are required to adequately describe the concentration of Salmonella in a lairage pen.

Variable abattoir conditions affect Salmonella enterica prevalence and meat quality in swine and pork.

Research suggests that abattoir holding pens pose significant Salmonella enterica risk to swine immediately preharvest. The goal of this study was to evaluate those factors related to holding that increased the prevalence of S. enterica in swine at slaughter. To accomplish this goal, we focused on holding time and flooring. Our objectives were to (1) compare Salmonella enterica prevalence among pigs held for short (15-45 min) versus long (up to 4 h) periods before slaughter; and (2) determine the impact of flooring (slatted vs. concrete) as it relates to the prevalence of S. enterica. The study consisted of seven repetitions at a large volume (11,000 head/day) Midwest abattoir. Each repetition consisted of one truck load of pigs (n = 170) sorted into one of three groups: (1) animals held for a short time (15-45 min) on solid floors (short-hold); (2) animals held for 4 +/- 0.5 h on slatted floors; and (3) animals held for 4 +/- 0.5 h on solid concrete floors. At slaughter, samples were collected from 30 pigs in each group. Cecal contents (20 mL), feces (20 g), and the ileocecal lymph node were cultured for S. enterica. Additionally, the effect of holding time on meat quality parameters (loin pH at 35 min and 6 h, color, drip loss) was evaluated for the first four replicates. The proportion of S. enterica-positive samples was highest (p < 0.05) in the cecum of pigs held on solid concrete floors (72.4%), and slightly less for pigs held on slatted floors (63.3%). Animals held for less than 45 min before slaughter demonstrated the lowest proportion of S. enterica-positive samples (52.9%). The pig prevalence, as measured by any one of the three samples being positive, was significantly different (p < 0.05) between animals held on solid floors (81%) and those animals held for 45 min or less before slaughter (69%). Meat quality, as measured by multiple parameters, was adversely affected by lack of a rest period. The mean 24-h pH was significantly lower for the short-hold group compared to the other two groups. The mean Minolta L and the drip loss were significantly higher in the short-hold group. From this and other studies, it appears that elimination of the holding process is not feasible S. enterica control option, given current U.S. harvesting systems.

Effect of short-term lairage on the prevalence of Salmonella enterica in cull sows.

This study was designed to compare Salmonella enterica prevalence in sows held in a holding pen at the abattoir for approximately 2 h (hold sows) with sows slaughtered immediately after transport to the abattoir (no-hold sows). Cull sows (n = 160) were sampled from four sampling periods over 8 weeks (February to March 2002) at the abattoir. Sows originated from an integrated swine farm and were sent to a live-hog market and then to the slaughter facility. Before testing, sows entered the abattoir pen and four 100-cm2 four-ply gauze squares were placed randomly on the pen floor for S. enterica culture. Sows were alternatively assigned to the hold or no-hold group. Samples collected from sows during slaughter were ileocecal lymph node, cecal contents, transverse colon contents, subiliac lymph node, sponge swabs of the left and right carcass section (300 cm2), and chopped meat. Overall, S. enterica was isolated from 44% (35 of 80) of the no-hold sows, which was significantly less (P < 0.05) than 59% (47 of 80) of the held sows. Also, no-hold sows had a lower cecal content prevalence (39%, 31 of 80) compared with that (55%, 44 of 80) of held sows (P < 0.05). S. enterica serovars isolated from no-hold sows were Brandenburg (n = 16), Derby (n = 12), Hadar (n = 8), Infantis (n = 6), Johannesburg (n = 3), 6,7:z10-monophasic (n = 3), and Typhimurium (n = 1). S. enterica serovars isolated from held sows (n = 61 isolates) were Derby (n = 19), 6,7: z10-monophasic (n = 15), Brandenburg (n = 10), Infantis (n = 6), Hadar (n = 5), Johannesburg (n = 4), and Tennessee (n = 2). Serovars recovered from the pen were Reading (n = 6), Derby (n = 4), Uganda (n = 2), and Manhattan (n = 2). Results of this study suggest that holding pens contribute to increased S. enterica carriage in cull sows. Abattoir holding pens might be an important control point for S. enterica in the ground pork production chain.

Estimation of the Salmonella enterica prevalence in finishing swine.

The study objective was to evaluate three methods of Salmonella enterica prevalence estimation in swine herds (faecal culture, culture of abattoir-collected samples, and serum ELISA). From each of six swine herds, we necropsied approximately 100 finishing pigs (> 70 kg); one-half on farm and the other half at the abattoir, after transport and approximately 2.5 h holding. We collected the same samples for S. enterica culture at both locations (1 g faecal, 10 g caecal contents, ileocaecal lymph nodes, superficial inguinal lymph nodes, 25 g of gluteal muscle for serum ELISA). On farm, the 1 g faecal sample only detected 13.3% (2/15) of all positive pigs necropsied on farm. However, with abattoir and on-farm results combined, the faecal sample detected 57.4% (74/129) of positive pigs. Abattoir-collected samples provided prevalence estimates much higher than on-farm collected samples (39.9 vs. 5.3%; P < 0.001). This study shows that faecal samples have a low sensitivity for detecting infected pigs and that abattoir-collected samples overestimate the on-farm S. enterica prevalence. For most herds, serology overestimated the on-farm culture prevalence.

Preslaughter holding environment in pork plants is highly contaminated with Salmonella enterica.

The objective of this study was to determine whether abattoir pens can provide a Salmonella enterica infection source during the 2 to 4 h of preharvest holding. Previous work has suggested that pigs may be getting infected, but little has been reported on the environmental contamination of abattoir holding pens. For 24 groups of pigs studied ( approximately 150 animals/group) at two high-capacity abattoirs, six pooled fecal samples (n, 10 per pool) were collected from each transport trailer immediately after pigs were unloaded. Holding pens were sampled (one drinking water sample and six pooled floor samples consisting of swabs, residual liquid, and feces) prior to entry of study pigs for the routine holding period ( approximately 2.5 h). After slaughter, cecal contents and ileocecal lymph nodes were collected, on the processing line, from 30 pigs in each studied group. All samples were cultured for the isolation and identification of S. enterica by primary enrichment in GN-Hajna and tetrathionate broths, secondary enrichment in Rappaport-Vassiliadis broth, and plating on brilliant green sulfa and xylose-lysine-tergitol-4 agars, followed by biochemical and serological identification. The study pens were highly contaminated with S. enterica; all holding pens sampled had at least one positive sample. Additionally, 33% (8 of 24) of drinking water samples were positive for S. enterica. All 24 groups of pigs had S. enterica-positive cecal contents and ileocecal lymph nodes, including those groups from transport trailers with no positive samples. From pigs, trailers, and pens, 586 isolates representing 36 different Salmonella serovars were isolated. Of the 353 isolates from pigs (109 from ileocecal lymph nodes plus 244 from cecal contents), 19% were identified as belonging to the same serovars as those isolated from the respective pens; 27% were identified as belonging to the same serovars as those isolated from the trailers. Sixteen percent of the unique serovars were isolated from both pigs and pens, suggesting that pens served as the infection source. This study demonstrates highly contaminated abattoir holding pens and watering sources. It also demonstrates that holding pens can serve as an infection source. This study identifies the abattoir holding pens as a significant hazard and a potential control point for Salmonella contamination in the preharvest pork production chain.

Impact of commercial preharvest transportation and holding on the prevalence of Salmonella enterica in cull sows.

The objective of this study was to examine the prevalence of Salmonella enterica in cull sows at various stages from the farm to the abattoir. Cull sows (n=181) were sampled over 10 weeks. Fecal samples (10 g each) were collected on the farm ca. 24 h before loading and at the live-hog market ca. 3 h before loading. Samples (ileocecal lymph nodes, cecal contents, feces from the transverse colon, ventral thoracic lymph nodes, subiliac lymph nodes, sponge swabs of the left and right carcass sections, and chopped meat) were collected at the abattoir. The percentages of positive fecal samples on the farm and at the live-hog market were 3% (5 of 181 samples) and 2% (3 of 181 samples), respectively. After transport from the live-hog market (10 h) and holding at the abattoir (6 h), 41% (74 of 180) of cull sows yielded S. enterica in one or more sampled tissues. The isolation rate for total cecal contents (33%; 60 of 180 samples) was significantly (P<0.05) higher than those for ileocecal lymph nodes (7%; 12 of 181 samples), feces (11%; 20 of 181 samples), and ventral thoracic and subiliac lymph nodes (2%; 4 of 181 samples). Before a 2% lactic acid carcass wash (lasting 8 to 9 s), 14% (25 of 180) of carcasses were positive, compared with 7% (12 of 179) after the wash (P<0.05). Two S. enterica serotypes, Derby and Infantis, were found on the farm and at the live-hog market. At the abattoir, 12 serotypes that had not previously been found on the farm or at the live-hog market were recovered. The results of this study demonstrate that transport and holding practices may contribute to an increase in S. enterica infection prior to slaughter to levels much higher than those found on the farm.

Salmonella enterica infections in market swine with and without transport and holding.

The objective of this study was to compare, by using identical sample types, the Salmonella enterica prevalences and serovar diversities between pigs necropsied on the farm and those necropsied at the abattoir after transport and holding. We necropsied 567 market weight pigs (>70 kg) from six herds. Pigs were alternately assigned to be necropsied on the farm or at the abattoir. One-half of the group was sent in clean, disinfected trailers to slaughter at a commercial abattoir. After transport (mean distance, 169 km) and 2 to 3 h of holding in antemortem pens, these pigs were necropsied. The 50 pigs remaining on the farm were necropsied the following day. The same sample types and amounts were collected for S. enterica culture at both locations. Results show a sevenfold-higher (P < 0.001) S. enterica isolation rate from pigs necropsied at the abattoir (39.9%; 114 of 286) than from those necropsied on the farm (5.3%; 15 of 281). This difference was also observed for each individual herd. All sample types showed a significantly higher prevalence when comparing abattoir to on-farm collection, respectively: lymph nodes, 9.15 versus 3.6%; cecal contents, 13.6 versus 1.8%; 1 g of fecal matter, 25.2 versus 0.7%. Recovery of additional serovars at the abattoir suggests the pigs are receiving S. enterica from extra-farm sources. This study demonstrates that rapid infection during transport, and particularly during holding, is a major reason for increased S. enterica prevalence in swine. This finding identifies the holding pen as an important S. enterica control point in the pork production chain.

Impact of commercial pre-harvest processes on the prevalence of Salmonella enterica in cull sows.

This study examined the impact of transport and lairage on Salmonella enterica prevalence in cull sows held under commercial conditions. Five sampling periods over 10 weeks yielded 181 selected culls. Fecal sample positives at the farm and collection point were 2% and 3%, respectively. After transport and lairage 41% of cull sows yielded S. enterica in one or more sampled tissues. A significant increase in cecal isolations was observed. The two S. enterica serotypes found at the production site and collection point were found at the abattoir, as were 13 other serotypes. Transport and lairage practices may contribute to an increased S. enterica contamination immediately prior to slaughter.

Experimental rapid infection in market swine following exposure to a Salmonella contaminated environment.

The objective of these experiments was to evaluate the possibility of swine becoming infected with Salmonella Typhimurium after a short time interval in a contaminated environment. Two experiments were conducted. Experiment 1 consisted of five trials with eight market weight swine. Pigs were necropsied at 2 (n = 10), 3 (n = 10) and 6 (n = 5) hours after continuous exposure to an environment contaminated with feces shed by swine intranasally inoculated with nalidixic acid-resistant Salmonella Typhimurium (chi 4232). In Experiment 2, pigs were necropsied after 30 minutes (n = 6), 60 minutes (n = 6), 2 hours (n = 6), and 6 hours (n = 3). In addition, control animals with no exposure were also necropsied in both experiments. At necropsy, the superficial inguinal, ileocecal, and mandibular lymph nodes, as well as cecal contents, distal ileum portion, and feces were evaluated. All samples were cultured for the presence of the nalidixic acid-resistant Salmonella. Feces deposited on the floor by intranasally inoculated swine were mixed with water to form slurry with a resulting load of 10(3)-10(5) Salmonella Typhimurium CFU per gram. In Experiment 1, 80% percent of animals with a 2-hour, 60% of animals with a 3-hour, and 100% of animals with a 6-hour exposure to this slurry had at least one sample test positive for the marked Salmonella Typhimurium strain. In Experiment 2, 50% of the 30 minute, 50% of the 60 minute, and 33% of the 2-hour exposed pigs had at least one sample test positive. These experiments show that market swine can become infected during routine resting or holding periods when exposed to relatively low levels (10(3) CFU) of Salmonella in the simulated pre-slaughter environment, and that exposure times as short as 30 minutes are sufficient to produce contaminated gastrointestinal tracts. They also demonstrate the high risk of holding pigs longer than six hours. Intervention at this step in the swine production process may have a significant impact on the safety of pork products.

Comparison of culture, multiplex, and 5' nuclease polymerase chain reaction assays for the rapid detection of Yersinia enterocolitica in swine and pork products.

Bacteriological culture was compared with multiplex and fluorogenic (TaqMan) polymerase chain reaction (PCR) assays for the detection of attachment invasion locus (ail)-bearing Yersinia enterocolitica in market weight swine, chitterlings, and ground pork. The TaqMan assay detected 1 pg of purified Y. enterocolitica DNA, whereas conventional gel-based PCR detected I ng of the same. The presence of ail-bearing Y. enterocolitica was tested in pork and feces artificially inoculated with Y. enterocolitica strain NADC 5561. The sensitivity limits of culture, multiplex, and TaqMan PCR assays were 4 x 10(3), 4 x 10(2), and 0.4 CFU/g, respectively, for the artificially inoculated pork. The sensitivity limits were 4 x 10(2), 4 x 10(2), and 0.4 CFU/g, respectively, for feces after a 48-h enrichment in a Yersinia selective broth. By the culture method, Y. enterocolitica was not detected in any of the swine specimens (n = 2,403) examined. By contrast, it was detected in 48 (2%) of the swine samples screened using the multiplex PCR and in 656 (27.2%) of these samples using the TaqMan assay. Using the culture method, Y. enterocolitica was detected in 8% of chitterling samples (n = 350) and in none of the ground pork samples (n = 350). It was identified in 27% of the chitterling samples using multiplex PCR and in 79% of these samples using the TaqMan assay. Ten percent of the ground pork samples contained Y. enterocolitica, as determined by the multiplex PCR, and 38% based on the TaqMan assay. The results suggest that pork products harbor more ail-bearing Y. enterocolitica than selected organs of freshly slaughtered hogs and that the TaqMan assay is more sensitive than either the multiplex PCR or traditional culture methods.

Rapid infection in market-weight swine following exposure to a Salmonella typhimurium-contaminated environment.

OBJECTIVE: To evaluate the possibility of swine becoming infected with Salmonella Typhimurium when housed for 2 to 6 hours in an environment contaminated with Salmonella, similar to a lairage situation prior to slaughter. ANIMALS: 40 crossbred market pigs with an approximate body weight of 92 kg. PROCEDURE: Five trials were conducted (8 pigs/trial) in simulated lairage conditions. Superficial inguinal, ileocecal, and mandibular lymph nodes, cecal contents, distal portion of the ileum, and fecal samples were obtained from each pig after 2 (n = 10), 3 (10), and 6 (5) hours of exposure to an environment contaminated with feces defecated by 10 pigs intranasally inoculated with nalidixic acid-resistant Salmonella Typhimurium (chi4232). In addition, 5 control pigs that were not exposed were also evaluated in the same manner. RESULTS: Feces deposited on the floor by intranasally inoculated swine were mixed with water to form slurry with a resulting load of approximately 10(3) colony-forming units of Salmonella Typhimurium/g of material. Eight of 10, 6 of 10, and 6 of 6 pigs exposed to the slurry for 2, 3, or 6 hours, respectively, had positive results for at least 1 sample when tested for the specific strain of Salmonella Typhimurium. CONCLUSIONS AND CLINICAL RELEVANCE: Pigs can become infected during routine resting or holding periods during marketing when exposed to relatively low amounts of Salmonella organisms in the preslaughter environment. Intervention at this step of the production process may have a major impact on the safety of pork products.

The effect of lairage on Salmonella isolation from market swine.

The objective of this paper was to evaluate the effect of lairage (holding >12 h during transport to slaughter) in clean facilities on Salmonella isolation from market swine. We tested 30 market-bound pigs (about 240 lb [110 kg]) on each of 10 occasions from an Iowa farrow-to-finish operation with about 600 sows. All pigs were slaughtered, and samples were collected at a large Midwest abattoir. On the farm, fecal samples were collected for culture of Salmonella. Pigs were alternately assigned to a lairage treatment (holding in a clean, disinfected facility at the National Animal Disease Center) group or a control group (remaining on the farm). After about 18 h, both groups were transported (about 137 km) to a large Midwest abattoir, commingled, and slaughtered. After slaughter, samples were collected for culture of Salmonella (feces from the distal colon, ileocecal lymph nodes, cecal contents, ventral thoracic lymph nodes, subiliac lymph nodes, and carcass swabs). Diaphragm sections were collected for serum ELISA. Salmonella enterica Derby was the only serotype isolated from farm fecal samples (3.4%, 10 of 290). Multiple serotypes (n = 17) were isolated from 71.8% (196 of 273) of the pigs when abattoir-collected samples were cultured: cecal contents (21.2%. 58 of 273), distal colon contents (52%, 142 of 273), and ileocecal lymph nodes (43.6%, 119 of 273). There were lower Salmonella isolation rates from the lairaged pigs (P < 0.05). The predominant serotype isolated at the abattoir varied by week of the study. This study suggests that pigs became internally contaminated with Salmonella after leaving the farm, possibly while in the abattoir holding pens, and that 18 h lairage, in clean facilities, does not increase shedding.

Management factors affecting the risk for vesicular stomatitis in livestock operations in the western United States.

OBJECTIVE: To identify management factors affecting the risk of animals developing vesicular stomatitis (VS). DESIGN: Case-control study. ANIMALS: Horses, cattle, and sheep with suspected vesicular stomatitis on 395 premises in Colorado, New Mexico, Utah, and Arizona. PROCEDURE: Data were collected during the VS outbreak of 1997. Diagnosticians interviewed livestock owners and completed a supplemental questionnaire. Cases were defined as those premises that had a completed questionnaire and had > or = 1 animal positive for VS. Control premises were all premises investigated that had a completed questionnaire and on which the animals had been tested but VS was not detected. RESULTS: Animals that had access to a shelter or barn had a reduced risk of developing VS (OR, 0.6; 95% confidence interval [CI], 0.35 to 0.99). This effect was more pronounced for equine premises (OR, 0.5; 95% CI, 0.3 to 0.9). Conversely, during an adjusted analysis on equine premises, risk of developing disease was increased slightly where animals had access to pasture (OR, 2.01; 95% CI, 1.1 to 3.7). On all premises where owners reported insect populations were greater than normal, odds of developing disease were significantly increased (OR, 2.5; 95% CI, 1.47 to 4.47). Premises with animals housed < 0.25 miles from running water were more than twice as likely to have clinical signs of VS (OR, 2.6; 95% CI, 1.32 to 5.0). CONCLUSIONS AND CLINICAL RELEVANCE: These results support reports of others that suggest biting insects are a vector in VS virus transmission. Management practices to reduce exposure to biting insects might reduce the risk of VS.

Detection of multiresistant Salmonella typhimurium DT104 using multiplex and fluorogenic PCR.

Salmonella infections continue to cause gastrointestinal and systemic disease throughout the world. Salmonella typhimurium DT104 further poses a major health concern due to its acquisition of resistance to multiple antibiotics. The rapid detection of multiresistant S. typhimurium DT104 would facilitate strategies aimed at controlling this pathogen. We developed a specific and sensitive polymerase chain reaction (PCR) assay that amplifies a segment of DNA that is conserved in multiresistant S. typhimurium DT104. To provide further specificity for this PCR-based diagnostic test, we amplified two other gene fragments that are present in S. typhimurium DT104. A multiplex PCR containing primers for targeted sequences resulted in the amplification of predicted size fragments from S. typhimurium DT104 exhibiting the ACSSuT (ampicillin, chloramphenicol, streptomycin, sulphamethoxazole and tetracycline) or ASSuT resistance phenotypes. A minor modification of the multiplex PCR enabled the detection of other related multiresistant Salmonella such as S. typhimurium U302. To augment the detection process, we also designed a fluorogenic PCR assay that can detect the DNA of multiresistant S. typhimurium DT104 in the presence of excess contaminating bacterial DNA. These results provide a method by which multiresistant S. typhimurium DT104, or potentially the next emerging multiresistant Salmonella, can be accurately detected in only 3-4 h.

1995 epizootic of vesicular stomatitis (New Jersey serotype) in the western United States: an entomologic perspective.

Entomologic and epizootic data are reviewed concerning the potential for transmission of vesicular stomatitis (VS) virus by insects, including field data from case-positive premises in New Mexico and Colorado during the 1995 outbreak of the New Jersey serotype (VSNJ). As with previous outbreaks of VSNJ in the western United States, the 1995 epizootic illustrated that risk of exposure is seasonal, increasing during warm weather and decreasing with onset of cool weather; virus activity spread from south to north along river valleys of the southwestern and Rocky Mountain states; clinical disease was detected most commonly in horses, but also occurred in cattle and 1 llama; and most infections were subclinical. Overall, 367 case-positive premises were identified during the 1995 outbreak, with foci of virus activity along the Rio Grande River south of Albuquerque, NM, in southwestern Colorado, and along the Colorado River near Grand Junction, CO. The establishment of a 16-km (10-mile) radius zone of restricted animal movement around confirmed positive premises, along with imposition of state and international embargoes, created economic hardship for livestock owners and producers. The importance of defining the role of blood-feeding insects as biological vectors of VSNJ virus relative to risk factors that promote high levels of insect transmission, such as the presence of livestock along western river valleys, blood feeding activity, and frequent transport of animals for recreational purposes, is emphasized as a basis for developing effective disease management.

Management and nutritional factors associated with the detection of Salmonella sp. from cattle fecal specimens from feedlot operations in the United States.

In a convenience sample of 100 feedlot operations (included in the United States Department of Agriculture: Animal and Plant Health Inspection Service 1994 Cattle on Feed Evaluation), up to 25 cattle fecal samples were collected and tested for the presence of Salmonella from each of two pens (the pen which contained the most-recent arrivals, and the pen with cattle that had been on feed the longest). One or more Salmonella spp. were recovered from 38 (38.0%) of the 100 feedlots, 52 (26.0%) of the 200 pens and 273 (5.5%) of the 4977 fecal samples collected. Multivariable logistic regression indicated that feeding tallow and feeding whole cottonseed or cottonseed hulls within seven days prior to fecal sample collection was associated with an increased risk of finding Salmonella in a pen. Variables not found to be significantly associated with the detection of Salmonella in a pen included region, operation size, use of sprinklers, time on feed, type of cattle in the pen, number and concentration of cattle in a pen, feeding probiotics, and various other feeds.