RESUMEN
Recent advancements in membrane technologies and disinfection methods have enhanced drinking water quality significantly. However, microorganisms, including free-living amoebae (FLA), persist and pose potential threats to humans. FLA are linked to severe neuro-ophthalmic infections and serve as hosts of pathogenic bacteria. This study examined FLA presence in chlorinated and ultrafiltration drinking water and evaluated chlorine's disinfectant. Of 115 water samples, 21 tested positive for Acanthamoeba sp., Allovahlkampfia sp., and Vermamoeba vermiformis, originating from chlorinated sources. FLA trophozoites withstand temperatures up to 37 °C, while the cysts tolerate heat shocks of 60-70 °C. Trophozoites are susceptible to 5 mg L-1 chlorine, but cysts remain viable at concentrations up to 10 mg L-1. FLAs' survival in chlorinated waters is attributed to high cyst tolerance and lower residual chlorine concentrations. These findings highlight the need for ultrafiltration or enhanced chlorination protocols to ensure safer drinking water.
Asunto(s)
Amoeba , Agua Potable , Halogenación , Amoeba/efectos de los fármacos , Agua Potable/química , Cloro/farmacología , Desinfección/métodos , Desinfectantes , Purificación del Agua/métodosRESUMEN
The production of biohythane, a combination of energy-dense hydrogen and methane, from the anaerobic digestion of low-cost organic wastes has attracted attention as a potential candidate for the transition to a sustainable circular economy. Substantial research has been initiated to upscale the process engineering to establish a hythane-based economy by addressing major challenges associated with the process and product upgrading. This review provides an overview of the feasibility of biohythane production in various anaerobic digestion systems (single-stage, dual-stage) and possible technologies to upgrade biohythane to hydrogen-enriched renewable natural gas. The main goal of this review is to promote research in biohythane production technology by outlining critical needs, including meta-omics and metabolic engineering approaches for the advancements in biohythane production technology.
Asunto(s)
Reactores Biológicos , Metano , Anaerobiosis , Fermentación , Hidrógeno/metabolismo , BiocombustiblesRESUMEN
Microcystins (MCs) are toxins produced by cyanobacteria commonly found in harmful algal blooms (HABs). Due to their toxicity to humans and other organisms, the World Health Organization (WHO) sets a guideline of 1 µg L-1 for microcystin-leucine-arginine (MC-LR) in drinking water. However, current analytical techniques for the detection of MC-LR such as liquid chromatography-mass spectrometry (LC-MS) and ELISA are costly, bulky, time-consuming, and mostly conducted in a laboratory, requiring highly trained personnel. An analytical method that can be used in the field for rapid determination is essential. In this study, an anti-MC-LR/MC-LR/cysteamine-coated screen-printed carbon electrode (SPCE) biosensor was newly developed to detect MC-LR, bioelectrochemically, in water. The functionalization of the electrode surface was confirmed with surface characterization methods. The sensor performance was evaluated by electrochemical impedance spectroscopy (EIS), obtaining a linear working range of MC-LR concentrations between 0.1 and 100 µg L-1 with a limit of detection (LOD) of 0.69 ng L-1. Natural water samples experiencing HABs were collected and analyzed using the developed biosensor, demonstrating the excellent performance of the biosensor with a relative standard deviation (RSD) of 0.65%. The interference tests showed minimal error and RSD values against other common MCs and possible coexisting ions found in water. The biosensor showed acceptable functionality with a shelf life of up to 12 weeks. Overall, the anti-MC-LR/MC-LR/cysteamine/SPCE biosensors can be an innovative solution with characteristics that allow for in situ, low-cost, and easy-to-use capabilities which are essential for developing an overarching and integrated "smart" environmental management system.
RESUMEN
In this study, a novel molybdenum disulfide (MoS2) nano-carbon (NC) coated cathode was developed for hydrogen production in a microbial electrolysis cell (MEC), while treating simulated urine with 2-6 times dilution (conductivity <20 mS cm-1). MoS2 nanoparticles were electrodeposited on the NC coated cathodes at -100, -150 and -200 µA cm-2 and their performances were evaluated in the MEC. The chronopotentiometry (CP) tests showed the improved catalytic activity of MoS2-NC cathodes with much lower cathode overpotential than non-MoS2 coated electrodes. The MoS2-NC200 cathode, electrodeposited at -200 µA cm-2, showed the maximum hydrogen production rate of 0.152 ± 0.002 m3 H2 m-2 d-1 at 0.9V of Eap, which is comparable to the previously reported Pt electrodes. It was found that high solution conductivity over 20 mS cm-1 (>600 mg L-1 NH3-N) can adversely affect the biofilm architecture and the bacterial activity at the anode of the MEC. Exoelectrogenic bacteria for this system at the anode were identified as Tissierella (Clostridia) and Bacteroidetes taxa. Maximum ammonia-nitrogen (NH3-N) and phosphorus (PO4 3--P) removal were 68.7 and 98.6%, respectively. This study showed that the newly fabricated MoS2-NC cathode can be a cost-effective alternative to the Pt cathode for renewable bioelectrochemical hydrogen production from urine.
RESUMEN
In this study, sliver (Ag) and gold (Au) nanoparticles (NPs) were embedded on poly (acrylic acid) (PAA)/poly (allylamine) hydrochloride (PAH) hydrogel fibers for improved electrochemical oxidation (EO) of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) removal. The NPs-loaded PAA/PAHs shows the better charge transport compared to the ceramic nanofiber membranes (CNM) electrodes. At 10 mA cm-2 of current density, the Ag-PAA/PAH electrodes showed a faster removal of PFAS compared to the Ag-CNM electrode probably due to large surface area-volume ratio and high porosity from the hydrogel. Among NPs-loaded PAA/PAH electrodes, the Ag/Au-PAA/PAH electrodes showed the highest removal of PFOA (72%) and PFOS (91%) in 2 h with the maximum removal rate of PFOA (0.0046 min-1) and PFOS (0.0093 min-1). The rapid PFOS removal is possibly due to the high activity of electron transfer with a higher redox potential of SO4â¢- than â¢OH. The highly stable F- generation was obtained from each electrode during reproducibility (n = 3). The net energy consumption from Ag/Au-PAA/PAH electrode was 164.9 kWh m-3 for 72% PFOA removal and 90 kWh m-3 for 91% PFOS removal, respectively. The developed Au-PAA/PAH electrodes were applied to lake water samples and showed acceptable PFOS removal (65%) with relative standard deviations (RSD) of 10.2% (n = 3) at 10 mA cm-2 of current density. Overall, the NP-embedded hydrogel nanofibers were proven to be a promising sustainable catalyst for the electrochemical PFAS oxidation in water.
Asunto(s)
Ácidos Alcanesulfónicos , Fluorocarburos , Nanopartículas , Caprilatos , Electrodos , Fluorocarburos/análisis , Hidrogeles , Oxidación-Reducción , Reproducibilidad de los Resultados , AguaRESUMEN
Bioaugmenting lignocellulose digestion with potent lignocellulolytic microbiomes (LMs) facilitates efficient biomethanation. Assessing the metabolic roles of microbial communities of the LMs and their complex interactions with the indigenous anaerobic digester microbiome is pivotal in implementing bioaugmentation. Multiple meta-omics are the frontline approaches to investigating gene functions, metabolic roles, and the ecological niches of LMs.
Asunto(s)
Lignina , Microbiota , Anaerobiosis , Lignina/metabolismoRESUMEN
A novel Au nanoparticle (AuNP)-biopolymer coated carbon screen-printed electrode (SPE) sensor was developed through the co-electrodeposition of Au and chitosan for mercury (Hg) ion detection. This new sensor showed successful Hg2+ detection in landfill leachate using square wave anodic stripping voltammetry (SWASV) with an optimized condition: a deposition potential of -0.6 V, deposition time of 200 s, amplitude of 25 mV, frequency of 60 Hz, and square wave step voltage of 4 mV. A noticeable peak was observed at +0.58 V associated with the stripping current of the Hg ion. The sensor exhibited a good sensitivity of ~0.09 µA/µg (~0.02 µA/nM) and a linear response over the concentration range of 10 to 100 ppb (50-500 nM). The limit of detection (LOD) was 1.69 ppb, which is significantly lower than the safety limit defined by the United States Environmental Protection Agency (USEPA). The sensor had an excellent selective response to Hg2+ in landfill leachate against other interfering cations (e.g., Zn2+, Pb2+, Cd2+, and Cu2+). Fifteen successive measurements with a stable peak current and a lower relative standard deviation (RSD = 5.1%) were recorded continuously using the AuNP-biopolymer-coated carbon SPE sensor, which showed excellent stability, sensitivity and reproducibility and consistent performance in detecting the Hg2+ ion. It also exhibited a good reliability and performance in measuring heavy metals in landfill leachate.
RESUMEN
Microbial fuel cells (MFCs) have recently been applied to generate electricity from oily wastewater. Although MFCs that utilize microalgae to provide a self-supporting oxygen (O2) supply at the cathode have been well discussed, those with microalgae at the anode as an active biomass for treating wastewater and producing electrons are still poorly studied and understood. Here, we demonstrated a bilgewater treatment using single- and double-chamber microalgal fuel cells (SMAFC and DMAFC) capable of generating energy with a novel microalgal strain (Chlorella sorokiniana) that was initially isolated from oily wastewater. Compared to previous MFC studies using green algae, relatively high voltage output (151.3-160.1 mV, 71.3-83.4 mV m-2 of power density) was observed in the SMAFC under O2 controlled systems (i.e., acetate addition or light/dark cycle). It was assumed that, under the O2 depletion, alternative electron acceptors such as bicarbonate may be utilized for power generation. A DMAFC showed better power density (up to 23.9%) compared to the SMAFC due to the separated cathode chamber which fully utilizes O2 as an electron acceptor. Both SMAFC and DMAFC removed 67.2-77.4% of soluble chemical oxygen demands (SCOD) from the synthetic bilgewater. This study demonstrates that the application of algae-based MFCs is a feasible strategy to treat oil-in-water emulsion while generating electricity.
RESUMEN
Biomethanation through anaerobic digestion (AD) is the most reliable energy harvesting process to achieve waste-to-energy. Microbial communities, including hydrolytic and fermentative bacteria, syntrophic bacteria, and methanogenic archaea, and their interspecies symbioses allow complex metabolisms for the volumetric reduction of organic waste in AD. However, heterogeneity in organic waste induces community shifts in conventional anaerobic digesters treating sewage sludge at wastewater treatment plants globally. Assessing the metabolic roles of individual microbial species in syntrophic communities remains a challenge, but such information has important implications for microbially enhanced energy recovery. This review focuses on the alterations in digester microbiome and intricate interspecies networks during substrate variation, symbiosis among the populations, and their implications for biomethanation to aid stable operation in real-scale digesters.
Asunto(s)
Microbiota/fisiología , Simbiosis/fisiología , Anaerobiosis , Archaea/fisiología , Bacterias , Fenómenos Fisiológicos Bacterianos , Reactores Biológicos/microbiología , Fermentación , Lípidos , Redes y Vías Metabólicas , Polisacáridos , Aguas del Alcantarillado/microbiología , Eliminación de Residuos Líquidos , Aguas Residuales , Purificación del AguaRESUMEN
Per- and poly-fluoroalkyl substances (PFASs) have recently been labeled as toxic constituents that exist in many aqueous environments. However, traditional methods used to determine the level of PFASs are often not appropriate for continuous environmental monitoring and management. Based on the current state of research, PFAS-detecting sensors have surfaced as a promising method of determination. These sensors are an innovative solution with characteristics that allow for in situ, low-cost, and easy-to-use capabilities. This paper presents a comprehensive review of the recent developments in PFAS-detecting sensors, and why the literature on determination methods has shifted in this direction compared to the traditional methods used. PFAS-detecting sensors discussed herein are primarily categorized in terms of the detection mechanism used. The topics covered also include the current limitations, as well as insight on the future direction of PFAS analyses. This paper is expected to be useful for the smart sensing technology development of PFAS detection methods and the associated environmental management best practices in smart cities of the future.
RESUMEN
A novel bismuth (Bi)-biopolymer (chitosan) nanocomposite screen-printed carbon electrode was developed using a Bi and chitosan co-electrodepositing technique for detecting multiple heavy metal ions. The developed sensor was fabricated with environmentally benign materials and processes. In real wastewater, heavy metal detection was evaluated by the developed sensor using square wave anodic stripping voltammetry (SWASV). The nanocomposite sensor showed the detection limit of 0.1 ppb Zn2+, 0.1 ppb Cd2+ and 0.2 ppb Pb2+ in stock solutions. The improved sensitivity of the Bi-chitosan nanocomposite sensor over previously reported Bi nanocomposite sensors was attributed to the role of chitosan. When used for real wastewater samples collected from a mining site and soil leachate, similar detection limit values with 0.4 ppb Cd2+ and 0.3 ppb Pb2+ were obtained with relative standard deviations (RSD) ranging from 1.3% to 5.6% (n = 8). Temperature changes (4 and 23 °C) showed no significant impact on sensor performance. Although Zn2+ in stock solutions was well measured by the sensor, the interference observed while detecting Zn2+ in the presence of Cu2+ was possibly due to the presence of Cu-Zn intermetallic species in mining wastewater. Overall, the developed sensor has the capability of monitoring multiple heavy metals in contaminated water samples without the need for complicated sample preparation or transportation of samples to a laboratory.
RESUMEN
Recent advancements in MoS2 nanofilms have aided in the development for important water-related environmental applications. However, a MoS2 nanofilm-coated sensor has yet to have been applied for heavy metal detection in water-related environmental samples. In this study, a novel vertically aligned two-dimensional (2D) MoS2 (edge exposed) nanofilm was applied for in situ lead ion (Pb2+) detection. The developed sensor showed an excellent linear relationship toward Pb2+ between 0 and 20 ppb at -0.45 V vs Ag/AgCl using square wave anodic stripping voltammetry (SWASV) with the improved limit of detection (LOD) of 0.3 ppb in a tap water environment. The vertically aligned 2D MoS2 sensor exhibited improved detection sensitivity (2.8 folds greater than a previous metallic [Bi] composite electrode) with lower relative standard deviation for repetitive measurements (n = 11), indicating enhanced reproducibility for Pb2+ detection. The vertically aligned 2D MoS2 layers exhibited 2.6 times higher sensitivity than horizontally aligned 2D MoS2 (basal plane exposed). Density functional theory calculations demonstrated that adsorption energy of Pb on the MoS2 side edge was much higher (4.11 eV) than those on the basal plane (0.36 and 0.07 eV). In addition, the band gap center of vertical MoS2 was found to be higher than the Pb2+ â Pb reduction potential level and capable of reducing Pb2+. Overall, the newly developed vertically aligned 2D MoS2 sensor showed excellent performance for detecting Pb2+ in a real drinking water environment with good reliability.
Asunto(s)
Agua Potable/análisis , Técnicas Electroquímicas/instrumentación , Técnicas Electroquímicas/métodos , Plomo/análisis , Nanoestructuras/química , Calibración , Teoría Funcional de la Densidad , Disulfuros/química , Electrodos , Diseño de Equipo , Límite de Detección , Metales Pesados/química , Molibdeno/química , Contaminantes Químicos del Agua/análisisRESUMEN
Effective remediation of bilge water, a shipboard oily liquid waste, is important for both commercial and military vessels due to the domestic and international regulations. In this study, bilge water was used as a substrate for exoelectrogenic bacteria and biodegradation of bilge water and concurrent electricity generation were investigated using Pseudomonas putida ATCC 49128 in single chamber microbial fuel cells (MFCs). To enhance bioavailability of the bilge water, two types of surfactants were added (100 ppm) into the oily wastewater containing 0.1% standard bilge mix (SBM) and their impacts on electricity production were evaluated under various conditions. Anionic surfactant (sodium dodecyl sulfate, SDS) addition increased soluble chemical oxygen demand (SCOD) by forming micelle, producing maximum power density of 225.3 ± 3.2 mW m-2. However, the MFC with nonionic surfactant (Triton X-100) produced only 2.3 ± 0.1 mW m-2 due to no enhancement on biodegradable SCOD. A high NaCl concentration (100-500 mM) adversely affected power production due to decrease in available SCOD caused by emulsion coalescence. This is a first study to use surfactants to enhance bioavailability of non-biodegradable oily wastewater in a single chamber MFC.
Asunto(s)
Fuentes de Energía Bioeléctrica , Octoxinol/química , Pseudomonas putida/metabolismo , Dodecil Sulfato de Sodio/química , Tensoactivos/química , Disponibilidad Biológica , Navíos , Cloruro de Sodio/farmacología , Aguas ResidualesRESUMEN
Microalgal biomass has received much attention as feedstock for biofuel production due to its capacity to accumulate a substantial amount of biocomponents (including lipid, carbohydrate, and protein), high growth rate, and environmental benefit. However, commercial realization of microalgal biofuel is a challenge due to its low biomass production and insufficient technology for complete utilization of biomass. Recently, advanced strategies have been explored to overcome the challenges of conventional approaches and to achieve maximum possible outcomes in terms of growth. These strategies include a combination of stress factors; co-culturing with other microorganisms; and addition of salts, flue gases, and phytohormones. This review summarizes the recent progress in the application of single and combined abiotic stress conditions to stimulate microalgal growth and its biocomponents. An innovative schematic model is presented of the biomass-energy conversion pathway that proposes the transformation of all potential biocomponents of microalgae into biofuels.
Asunto(s)
Microalgas , Biocombustibles , Biomasa , Carbohidratos , LípidosRESUMEN
Microalgae can offer several benefits for wastewater treatment with their ability to produce large amounts of lipids for biofuel production and the high economic value of harvested biomass for biogas and fertilizer. This study found that salt concentration (â¼45gL-1) had more of an effect than salt type on metabolisms of Chlorella vulgaris for wastewater treatment and biofuel production. Salinity stress decreased the algal growth rate in wastewater by 0.003day-1permScm-1 and slightly reduced nutrient removal rates. However, salinity stress was shown to increase total lipid content from 11.5% to 16.1% while also increasing the saturated portions of fatty acids in C. vulgaris. In addition, salinity increased the algal settling rate from 0.06 to 0.11mday-1 which could potentially reduce the cost of harvesting for algal biofuel production. Overall, C. vulgaris makes a suitable candidate for high salinity wastewater cultivation and biofuel production.
Asunto(s)
Biocombustibles , Chlorella vulgaris , Biomasa , Chlorella , Lípidos , Microalgas , Aguas ResidualesRESUMEN
Effect of various flue gas CO2 concentrations (5%, 10% and 14.1%) on growth rate and biochemical properties of a green microalga Scenedesmus obliquus was investigated. S. obliquus showed the highest biomass production and growth rate (0.36â gâ L-1 and µmax = 1.00â day-1), total inorganic carbon removal (35.8â mgâ L-1), lipid productivity (9.9â mgâ L-1â day) and carbohydrate productivity (10.3â mgâ L-1â day) with 14.1% CO2 after 8 days of cultivation. Fatty acid methyl ester analysis revealed that the palmitic and oleic acid contents were increased up to 5% and 7% with 14.1% CO2, respectively. Application of flue gas CO2 enhanced the growth along with lipid and carbohydrate productivity of S. obliquus, which can be exploited for reducing the CO2 concentration.
Asunto(s)
Biocombustibles , Dióxido de Carbono , Scenedesmus , Biomasa , Carbohidratos , Ácidos Grasos , MicroalgasRESUMEN
Microalgae species including Chlamydomonas mexicana, Micractinium reisseri, Scenedesmus obliquus and Tribonema aequale were cultivated in batch cultures, and their biochemical composition was determined. C. mexicana showed the highest carbohydrate content of 52.6% and was selected for further study. Sonication pretreatment under optimum conditions (at 40 kHz, 2.2 Kw, 50 °C for 15 min) released 74 ± 2.7 mg g(-1) of total reducing sugars (TRS) of dry cell weight, while the combined sonication and enzymatic hydrolysis treatment enhanced the TRS yield by fourfold (280.5 ± 4.9 mg g(-1)). The optimal ratio of enzyme [E]:substrate [S] for maximum TRS yield was [1]:[5] at 50 °C and pH 5. Combined sonication and hydrolysis treatment released 7.3% (27.1 ± 0.9 mg g(-1)) soluble protein of dry cell weight, and further fermentation of the dissolved carbohydrate fraction enhanced the soluble protein content up to 56% (228.4 mg g(-1)) of total protein content. Scanning and transmission electron microscopic analyses indicated that microalgae cells were significantly disrupted by the combined sonication and enzyme hydrolysis treatment. This study indicates that pretreatment and subsequent fermentation of the microalgal biomass enhance the recovery of carbohydrates and proteins which can be used as feedstocks for generation of biofuels.
Asunto(s)
Biomasa , Chlorophyta/crecimiento & desarrollo , Microalgas/crecimiento & desarrollo , Proteínas de Plantas , Polisacáridos , Proteínas de Plantas/biosíntesis , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Polisacáridos/biosíntesis , Polisacáridos/química , Polisacáridos/aislamiento & purificaciónRESUMEN
A novel isolated bacterium Rhodococcus sp. YSPW03 was able to reduce high concentrations (up to 700 mg L(-1)) of perchlorate using acetate as electron donor. Perchlorate reduction rate increased from 2.90 to 11.23 mg L(-1) h(-1) with increasing initial acetate concentration from 100 to 2000 mg L(-1), leading to complete removal of perchlorate (100 mg L(-1)) within 9 h. The bacterium also promoted complete reduction of high perchlorate concentrations (500 and 700 mg L(-1)) at 2000 mg L(-1) of acetate within 48 and 96 h, respectively. Under semi-continuous reactor operation, efficient reduction on varied perchlorate concentrations (80-700 mg L(-1)) was performed by the bacterium in presence of acetate (600-6000 mg L(-1)) over 140 days. The highest perchlorate reduction rate of 280 mg L(-1) day(-1) was observed with an initial perchlorate concentration of 570 mg L(-1) at day 34. Dissolved chloride ions of 1000 mg L(-1) in the semi-continuous reactor (SCR) completely inhibited the biological perchlorate reduction. The findings of this study will help improve the perchlorate bioreactor design and determine the optimal conditions to maximize the perchlorate reduction efficiency.
Asunto(s)
Percloratos/metabolismo , Rhodococcus/metabolismo , Contaminantes Químicos del Agua/metabolismo , Reactores BiológicosRESUMEN
Eukaryotic algae and cyanobacteria produce hydrogen under anaerobic and limited aerobic conditions. Here we show that novel microalgal strains (Chlorella vulgaris YSL01 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydrogen through photosynthesis, using CO2 as the sole source of carbon under aerobic conditions with continuous illumination. We employ dissolved oxygen regimes that represent natural aquatic conditions for microalgae. The experimental expression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16 enzymatically produce hydrogen, even under atmospheric conditions, which was previously considered infeasible. Photoautotrophic H2 production has important implications for assessing ecological and algae-based photolysis.
Asunto(s)
Chlorella vulgaris/metabolismo , Hidrógeno/metabolismo , Microalgas/metabolismo , Fotosíntesis , Aerobiosis , Procesos Autotróficos , Oxígeno/metabolismoRESUMEN
BACKGROUND: Microalgal biomass contains a high level of carbohydrates which can be biochemically converted to biofuels using state-of-the-art strategies that are almost always needed to employ a robust pretreatment on the biomass for enhanced energy production. In this study, we used an ultrasonic pretreatment to convert microalgal biomass (Scenedesmus obliquus YSW15) into feasible feedstock for microbial fermentation to produce ethanol and hydrogen. The effect of sonication condition was quantitatively evaluated with emphases on the characterization of carbohydrate components in microalgal suspension and on subsequent production of fermentative bioenergy. METHOD: Scenedesmus obliquus YSW15 was isolated from the effluent of a municipal wastewater treatment plant. The sonication durations of 0, 10, 15, and 60 min were examined under different temperatures at a fixed frequency and acoustic power resulted in morphologically different states of microalgal biomass lysis. Fermentation was performed to evaluate the bioenergy production from the non-sonicated and sonicated algal biomasses after pretreatment stage under both mesophilic (35°C) and thermophilic (55°C) conditions. RESULTS: A 15 min sonication treatment significantly increased the concentration of dissolved carbohydrates (0.12 g g(-1)), which resulted in an increase of hydrogen/ethanol production through microbial fermentation. The bioconvertibility of microalgal biomass sonicated for 15 min or longer was comparable to starch as a control, indicating a high feasibility of using microalgae for fermentative bioenergy production. Increasing the sonication duration resulted in increases in both algal surface hydrophilicity and electrostatic repulsion among algal debris dispersed in aqueous solution. Scanning electron microscope images supported that ruptured algal cell allowed fermentative bacteria to access the inner space of the cell, evidencing an enhanced bioaccessibility. Sonication for 15 min was the best for fermentative bioenergy (hydrogen/ethanol) production from microalga, and the productivity was relatively higher for thermophilic (55°C) than mesophilic (35°C) condition. CONCLUSION: These results demonstrate that more bioavailable carbohydrate components are produced through the ultrasonic degradation of microalgal biomass, and thus the process can provide a high quality source for fermentative bioenergy production.