Quality of Life in Adults with Metabolic Dysfunction-Associated Fatty Liver Disease.

The aims of this study were as follows: to investigate the association between metabolic dysfunction-associated fatty liver disease (MAFLD) and health-related quality of life (HRQoL), to evaluate whether stress perception and mental health among patients with MAFLD affect HRQoL, and to identify the underrated burden on MAFLD patients. Nationwide data from the 5th Korean National Health and Nutrition Examination Survey (KNHANES V, 2010 to 2012) were used. MAFLD was defined by a fatty liver index (FLI) of ≥60, and the EuroQol-5D (EQ-5D) was used to assess HRQoL. Logistic regression analysis and odds ratios (ORs) were used to determine the associations of MAFLD with stress, mental health, and HRQoL. Previous suicidal impulse was not found to be significantly associated with HRQoL. The risk of MAFLD increased 1.265-fold with an increase in stress levels based on the stress perception rate (confidence index (CI): 1.046-1.530; p < 0.05), while it increased 1.091-fold with a 1-point decrease in the EQ-5D score (CI: 1.019-1.169; p < 0.05). HRQoL impairment and stress levels are associated with MAFLD. It is important to evaluate stress levels among MAFLD patients and implement stress management and HRQoL improvement strategies.

A multifunctional, one-step gas foaming strategy for antimicrobial silver nanoparticle-decorated 3D cellulose nanofiber scaffolds.

Creating nanoparticle-decorated nanofibers in a single step can greatly speed up and scale up the production of scaffolds for various applications. In this study, we report a facile multifunctional method for the simultaneous foaming and synthesis of silver nanoparticles-covered three-dimensional cellulose using sodium borohydride (NaBH4). The physicochemical properties of the 3D cellulose-Ag scaffold were evaluated and compared to 2D CA membranes, including morphology (porous 3D vs flat 2D), mechanical properties (22.72 vs <13 MPa Young's modulus), antibacterial effect (27 vs 0 mm zone of inhibition), and biocompatibility. The findings suggest that our method enables the scaffold to be easily manufactured-indicating it can be used to scale-up manufacturing processes-with high bioactivity, antibacterial effect, and biocompatibility, showing potential as a 3D structure production method for tissue engineering and other relevant applications.

Simple conversion of 3D electrospun nanofibrous cellulose acetate into a mechanically robust nanocomposite cellulose/calcium scaffold.

Cellulose and its derivatives are widely used as nanofibrous biomaterials, but obtaining 3D cellulose nanofibers is difficult and relevant research is scarce. In the present study, we propose a simple method for converting electrospun 3D cellulose acetate/lactic acid nanofibers via calcium hydroxide treatment into a 3D cellulose/calcium lactate nanocomposite matrix. The conversion resulted in producing a stronger nanofibrous matrix (1.382 MPa vs. 0.112 MPa) that is more hydrophilic and cell-friendly compared to the untreated cellulose acetate/lactic acid group. The successful conversion was verified via FTIR, XPS, TGA, DTG, and XRD. The ability of the scaffolds to provide a suitable environment for cell growth and infiltration was verified by CCK assay and confocal microscopy. The porous nature, mechanical strength, and presence of calcium make the 3D cellulose/calcium lactate matrix a promising material for bone tissue engineering.

Quartz Crystal Nanobalance-Dissipation Based Simulation Model as Pre-Clinical Modality for Blood Coagulation Behavior for Evaluation of the Risk of Thrombosis.

In search of ideal treatment for coronary artery disease (CAD), various types of stents for preventing closure or stenosis have been developed. In particular, drug-eluting stents (DES), the most recently introduced class of coronary stents, has significantly reduced the incidence of restenosis by inhibiting hyperplasia of the intimal layer at the reperfusion site. On the other hand, recent studies reported the potential adverse effects of anti-proliferative drugs loaded on DES. For example, the patient group after DES use showed an increased incidence of late and very late stent thrombosis (after 30 days from PCI) compared to traditional bare-metal stents. Stent thrombosis which means a formation of blood clots is a complication following a PCI procedure. While the incidence rate of the complication is relatively low, the mortality of the complication is significant. Thus, the preclinical prediction model for arterial stenosis after deploying stents is required to reduce the adverse effect. Herein, we proposed a preclinical analytic method using QCN-D (Quartz Crystal Nanobalance-Dissipation) sensor for investigating the blood coagulation behavior on the dynamic condition of the stent surfaces. The quartz crystals (QCs) were coated with nitinol, heparin, and anti-proliferative drug to simulate the hemodynamic conditions on the surface of stents. The results of QCN-D demonstrated the potentiality as a preclinical system for evaluating the risks of stent thrombosis.

Fabrication of Three-Dimensional Alginate Porous Scaffold Incorporated with Decellularized Cornu Cervi Pantotrichum Particle for Bone Tissue Engineering.

Deer antler velvet (DAV), Cornu Cervi Pantotrichum, has been known for the outstanding growth rate and used in extracted liquid form in oriental herbal medicine for the tissue regeneration. The DAV is also famous for the abundance of many different minerals, proteins, growth factors and interleukins. The immense amount of DAV is consumed to produce DAV extract in Asian countries. However, the mechanical strength and the morphologic features of DAV have been overlooked. In this study, we revisited the possibility of DAV as a bone tissue scaffold. We first obtained DAV particles via physical decellularization followed by levigation procedure and then applied to the fabrication of three-dimensional porous alginate/DAVP sponge through lyophilizing alginate/DAVP hydrogel as a potential bone tissue scaffold source. The morphological and physicochemical properties of alginate/DAVP sponge were characterized using UTM, SEM, FE-SEM, and FT-IR. The alginate-based highly porous sponge demonstrated the interconnected porous structure with DAVP and improved mechanical properties. We expected both alginate/DAVP and DAVP are potential for tissue engineering application.

Fabrication of Bioabsorbable Polylactic-Co-Glycolic Acid/Polycaprolactone Nanofiber Coated Stent and Investigation of Biodegradability in Porcine Animal Model.

Stent-mediated therapy is minimally invasive and fairly effective for the specific tissue and organs with tubal structures such as the esophagus, intestine, and blood vessels. Cerebral arteries are one of the most critical tubal structures to maintain the physiological function and the life of the human. Since the retrieval of the implanted vascular stent is difficult and risky, the one-step stent therapy is imperative. However, the placement of a current pipe-typed stent can also limit the nutritional supply to the vascular wall. Also, the non-degradable polymeric layer is possibly sensitized to the recipient as a foreign body after prolonged period after implantation. Herein, we developed PLGA/PCL nanofiber-coated stent for blocking the flow towards the aneurysm cavity as well as allowing nutritional support to the vessel with the biodegradability. The PLGA/PCL nanofiber-coated stent (NCS) was fabricated via electrospinning composite nanofibers onto a self-expandable mater metal stent. The as-fabricated NCS was physicochemically characterized using FT-IR, FE-SEM, and UTM, and experimented in vivo as implanted in porcine models and radiologically and histologically analyzed. The NCS demonstrated improved physicochemical properties for intracranial aneurysmal treatment including enhanced mechanical properties. The bioabsorbability of NCS was confirmed in the animal model.

In Situ Biological Transmutation of Catalytic Lactic Acid Waste into Calcium Lactate in a Readily Processable Three-Dimensional Fibrillar Structure for Bone Tissue Engineering.

A bioinspired three-dimensional (3D) fibrous structure possesses biomimicry, valuable functionality, and performance to scaffolding in tissue engineering. In particular, an electrospun fibrous mesh has been studied as a scaffold material in various tissue regeneration applications. We produced a low-density 3D polycaprolactone/lactic acid (LA) fibrous mesh (3D-PCLS) via the novel lactic-assisted 3D electrospinning technique exploiting the catalytic properties of LA as we reported previously. In the study, we demonstrated a strategy of recycling the LA component to synthesize the osteoinductive biomolecules in situ, calcium lactate (CaL), thereby forming a 3D bioactive PCL/CaL fibrous scaffold (3D-SCaL) for bone tissue engineering. The fiber morphology of 3D-PCLS and its packing degree could have been tailored by modifying the spinning solution and the collector design. 3D-SCaL demonstrated successful conversion of CaL from LA and exhibited the significantly enhanced biomineralization capacity, cell infiltration and proliferation rate, and osteoblastic differentiation in vitro with two different cell lines, MC3T3-e1 and bone marrow stem cells. In conclusion, 3D-SCaL proves to be a highly practical and accessible strategy using a variety of polymers to produce 3D fibers as a potential candidate for future regenerative medicine and tissue engineering applications.

Fabrication of 3D Electrospun Polycaprolactone Sponge Incorporated with Pt@AuNPs for Biomedical Applications.

Here, we report the synthesis of three-dimensional (3D) polycaprolactone (PCL) nanofiber incorporated with core-satellite platinum nanoparticles (PtNPs, 2-3 nm) coated gold nanospheres (AuNPs, 30 nm) via the simple lactic acid assisted self-assembly electrospinning technique. The Pt-AuNPs nanoparticle in core-satellite form has been prepared by following solution based methods and characterized with TEM, HR-TEM, UV-Visible, and XRD spectroscopic techniques. The surface morphology and structural analysis of 3D nanofiber scaffolds have been performed with FTIR, TGA, FESEM, and HR-TEM analysis techniques and shown the successful preparation of 3D electrospun fibrous structure composed of Pt-AuNPs loaded PCL (PCL@Pt-AuNPs) as a potential biomaterial for bone tissue engineering applications.

Development of Highly Expandable Wrinkled Nanofiber Mat Using Metal Bundle Collector.

Nanofibers are used to improve performance in various fields such as biosensors and medical scaffolds. However, when it is used closely with a biological tissue, it is difficult to properly implement the original function due to the deformation caused by the constant movement of tissue. In this study, we developed a wrinkled nanofiber mats with excellent expandability using a novel metal bundle collector. The wrinkled nanofiber mats are composed of aligned nanofiber and random nanofiber repeated at regular intervals, and can move appropriately in response to the strain as the wrinkles formed in the aligned region can be wrinkled and spread.

In Situ Synthesis of Uprightly Grown Hierarchical Multi-Metallic Nano-Needles on Electrospun Fiber Surface.

In the thriving field of nano-materials science, the nanofibrous structure with immobilizednanoparticles (NPs) inside or outside of the fibers currently received great interest for their potential synergic effects. In the cooperative combining strategy, electrospun nanofibers in a tailored manner are promising materials as a platform to mutually gain both advantages of the interconnected fibrous structure and unique properties of NPs. Herein, we presented the one-step fabrication method for in-situ synthesis of novel uprightly stood extra-long hierarchical multi-metallic (Fe/Cu/S) nano-needles (NNs) on polyurethane (PU) electrospun nanofibers (PU@FeCuS). The multi-metallic nanostructures (FeCuSNNs) were in-situ synthesized by simply immersing PU nanofiber into the supersaturated FeSO4/CuSO4 solution. The results of SEM, FE-SEM, EDX mapping and 3D rendering model simulation with CAE software revealed the morphology, the composition of FeCuSNNs, and the significantly increased surface area. The possible crystallization mechanism of the formation of NNs was discussed.

Synthesis of polypyrrole nanorods via sacrificial removal of aluminum oxide nanopore template: A study on cell viability, electrical stimulation and neuronal differentiation of PC12 cells.

Synthesis of nanomaterials having uniform shape and size is a challenging task. Properties exhibited by such substrates would be compatible and homogeneous compared to the average properties displayed by those substrates with heterogeneous size. Herein, we report the synthesis of polypyrrole nanorods (PPy-NRs) of almost uniform size via oxidative chemical polymerization of pyrrole within anodized aluminum oxide nanopores followed by sacrificial removal of the template. Field emission scanning electron microscopy (FE-SEM), fourier transformed infra-red (FT-IR) spectra, X-ray diffraction (XRD), and ultra-violet-visible-near infra-red (UV-Vis-NIR) spectra of the substrate were used to analyze the physicochemical properties of as-synthesized PPy-NRs. PPy-NRs treated MC3T3-E1 and PC12 cells exhibited good biocompatibility in CCK-8 and live/dead assays. The assay showed more cell viability on PC12 cell lines. Electrical stimulation through PPy-NRs treated PC12 cells accelerated neuronal differentiation compared to those without electrical stimulation during in vitro cell culture.

QCN-Based Analysis for Predicting the Quality of Resulting Electrospun Nanofiber: Effect of Real-Time Transient Rheological Properties of Precursor Solution on Electrospinning.

There is a large margin between the mechanical properties and morphology of electrospun fibers required in each area. The produced fibers show a large difference depending on the external environment such as temperature, humidity, and season even in optimum concentration and same electrospinning set-up. The properties of polymer solution among the parameters are the largest determinant of the mechanical strength and diameter of electrospun fibers. Herein, the accurate predicting system in advance to electrospinning is required. In this study, we conduct a comparative study on the viscosity (measured by Brookfield rheometer) and the transient mass change and evaporation speed by our lab-made QCN in order to establish a predicting system for the quality of fiber. It was possible to measure the change of mass of the polymer solution in real-time using the lab-made QCN as well as calculating the volatility, the evaporation time of the polymer solution. The volatility of the polymer solution has a significant impact on the quality of the electrospun fiber including the diameter, uniformity, and surface topography. We compare the mass changes, viscosimetric viscosities, and the quality of corresponding fiber, and reveal the potential of QCN as a tool predicting pre-electrospinning fibers.

pH/NIR-Responsive Polypyrrole-Functionalized Fibrous Localized Drug-Delivery Platform for Synergistic Cancer Therapy.

Localized drug-delivery systems (LDDSs) are a promising approach for cancer treatment because they decrease systematic toxicity and enhance the therapeutic effect of the drugs via site-specific delivery of active compounds and possible gradual release. However, the development of LDDS with rationally controlled drug release and intelligent functionality holds great challenge. To this end, we have developed a tailorable fibrous site-specific drug-delivery platform functionalized with pH- and near-infrared (NIR)-responsive polypyrrole (PPy), with the aim of cancer treatment via a combination of photothermal ablation and chemotherapy. First, a paclitaxel (PTX)-loaded polycaprolactone (PCL) (PCL-PTX) mat was prepared by electrospinning and subsequently in situ membrane surface-functionalized with different concentrations of PPy. The obtained PPy-functionalized mats exhibited excellent photostability and heating property in response to NIR exposure. PPy-coated mats exhibited enhanced PTX release in a pH 5.5 environment compared to pH 7.4. Release was further accelerated in response to NIR under both conditions; however, superior release was observed at pH 5.5 compared to pH 7.4, indicating a dual stimuli-responsive (pH and NIR) drug-delivery platform. More importantly, the 808 nm NIR irradiation enabled markedly accelerated PTX release from PPy-coated PCL-PTX mats and slowed and sustained release following termination of laser irradiation, confirming representative stepwise drug-release properties. PPy-coated PCL-PTX mats presented significantly enhanced in vitro and in vivo anticancer efficacy under NIR irradiation compared to PPy-coated PCL-PTX mats not exposed to NIR or uncoated mats (PCL-PTX). This study has thus developed a promising fibrous site-specific drug-delivery platform with NIR- and pH-triggering that notably utilizes PPy as a dopant for synergistic photothermal chemotherapy.

Development of Nanofiber Reinforced Double Layered Cabin Air Filter Using Novel Upward Mass Production Electrospinning Set Up.

Recently, numerous researchers are interested in the development of new air filter because of air pollution caused by rapid industrialization and urbanization. The major concerns in developing air filters are: pressure drop and filtration efficiency which are considered significant. As the pressure drop increases, the energy consumption becomes high. In this study, we developed a novel air filter (polyurethane fiber mat) for nano size filtration using a mass production electrospinning, which is expected to enhance filtration efficiency and pressure drop effects. To determine the optimal electrospinning conditions for filter efficiency, various concentrations (8, 10, 12 wt/wt%) of thermoplastic polyurethane were prepared and employed. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FT-IR) were used for fiber characterization, and finally, efficiency test was conducted to evaluate the filter performance of developed nanofiber-based air filter. From this study, it could be concluded that optimization by adjusting the polymer concentration and electrospinning operating condition was the best efficient alternative method to fabricate nano-fibrous air filter system with improved filtration performance.

Bioengineered Osteoinductive Broussonetia kazinoki/Silk Fibroin Composite Scaffolds for Bone Tissue Regeneration.

In this article, Broussonetia kazinoki (BK) powdery extract is utilized to modify the silk fibroin (SF) scaffold and applied to the bone defect area. The BK/SF scaffold is an efficient cell carrier which promotes cell proliferation and osteogenic differentiation of rBMSCs (bone marrow derived mesenchymal stem cells). We confirmed biocompatibility and osteogenic differentiation capacity of BK/SF scaffolds compared to pristine SF scaffold in both in vitro and in vivo evaluation. Gene expression related to osteogenic differentiation and bone regeneration significantly upregulated in the BK/SF scaffold group. The implanted scaffolds were attached well to the surface of the bone defect region and integrated with surrounding tissues without significant inflammatory reaction. Furthermore, almost 45% of bone volume has been recovered at 8 weeks postsurgery, while the SF and control group showed 20% recovery. These results suggest that BK powdery extract incorporated with an SF scaffold might be a suitable substitute for an alternative bone graft for bone regeneration.

A Controlled Design of Aligned and Random Nanofibers for 3D Bi-functionalized Nerve Conduits Fabricated via a Novel Electrospinning Set-up.

Scaffolds made of aligned nanofibers are favorable for nerve regeneration due to their superior nerve cell attachment and proliferation. However, it is challenging not only to produce a neat mat or a conduit form with aligned nanofibers but also to use these for surgical applications as a nerve guide conduit due to their insufficient mechanical strength. Furthermore, no studies have been reported on the fabrication of aligned nanofibers and randomly-oriented nanofibers on the same mat. In this study, we have successfully produced a mat with both aligned and randomly-oriented nanofibers by using a novel electrospinning set up. A new conduit with a highly-aligned electrospun mat is produced with this modified electrospinning method, and this proposed conduit with favorable features, such as selective permeability, hydrophilicity and nerve growth directional steering, were fabricated as nerve guide conduits (NGCs). The inner surface of the nerve conduit is covered with highly aligned electrospun nanofibers and is able to enhance the proliferation of neural cells. The central part of the tube is double-coated with randomly-oriented nanofibers over the aligned nanofibers, strengthening the weak mechanical strength of the aligned nanofibers.