RESUMEN
A system for monitoring and analyzing the effectiveness and efficiency of the performance of the Board of the Federal Inspectorate for the Protection of Consumer Rights and Human Welfare in the Novosibirsk Region was introduced into its activities to estimate the provision of the Novosibirsk Region's population with sanitary epidemiological wellbeing in 2007-2009. The introduction of monitoring was ascertained to increase the effectiveness of budgetary fund surveillance and spending, by predicting the effectiveness and choice of priority lines of activities, by increasing the quality of budgetary services rendered in the provision of sanitary and epidemiological well-being to the population.
Asunto(s)
Comportamiento del Consumidor/legislación & jurisprudencia , Saneamiento/normas , Bienestar Social/legislación & jurisprudencia , Humanos , Estudios Retrospectivos , SiberiaAsunto(s)
Exposición a Riesgos Ambientales/efectos adversos , Enfermedades Ambientales/etiología , Estado de Salud , Medicina Social/métodos , Áreas de Influencia de Salud , Enfermedades Ambientales/epidemiología , Monitoreo del Ambiente/métodos , Monitoreo Epidemiológico , Sustancias Peligrosas/efectos adversos , Indicadores de Salud , Humanos , Federación de Rusia/epidemiología , Medicina Social/organización & administraciónRESUMEN
The content of 1-LTR and 2-LTR circular forms of HIV-1 proviral DNA was determined by nested PCR. Outer and inner primers for the first and second stages of PCR were selected in the env and gag regions that allowed the authors to simultaneously test 1- and 2 LTR of DNA HIV-1 forms in the analyzed samples. The accumulation of circular species of HIV-1 DNA was shown to occur during HIV infection as well for acutely infected cell lines as chronically infected mononuclear cells. The cell cultures providing productive infection was characterized by higher levels of 2-LTR circular HIV-1 DNA. Analysing the clinical samples demonstrated greater differences in the accumulation of the circular forms of proviral DNA. It was detected in 16 tested clinical samples both of 1-LTR and 2-LTR circular DNA in 6 samples and 1-LTR in 11 samples of 2-LTR of HIV-1 DNA is a minor fraction in most clinical samples, but in some samples the relative content of 1-LTR/2-LTR DNA was shown to be 1:1.
Asunto(s)
ADN Viral/análisis , Infecciones por VIH/virología , Duplicado del Terminal Largo de VIH/genética , VIH-1/genética , Provirus/genética , Línea Celular , Cartilla de ADN/química , Electroforesis en Gel de Poliacrilamida , Genoma Viral , Infecciones por VIH/patología , Humanos , Reacción en Cadena de la PolimerasaRESUMEN
New, so far unknown epidemically active natural foci of tick-borne rickettsiosis have been identified in Suzun District, Novosibirsk Province. The patients had a typical clinical pattern and epidemiological history of the disease. The cases were recorded in a number of communities in the northern forest-steppe zones. The results of the epidemiological survey and rickettsial studies of Ixodes ticks and blood sera of farm animals demonstrate the existence of clear-cut natural foci of tick-borne rickettsiosis, with its main vectors being ticks D. silvarum. The infestation of ticks was proved by biotests in passive hemagglutination tests, using an immunoglobulin diagnostic kit. Some measures for the monitoring of foci and disease prevention are suggested.
