RESUMEN
The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50â¯â¯mgâ¯L-1. Next, the sperm thawed at 39-40⯰C for 3â¯min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6â¯h after fertilization, while the hatching rate was examined 60â¯h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteochilus vittatus eggs (Pâ¯<â¯0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30â¯mgL-1 of glutathione (63.00⯱â¯5.89), however, this value was not significantly different from using concentration of 10 and 20â¯â¯mgâ¯L-1. A higher fertilization rate was produced using glutathione concentration of 50â¯â¯mgâ¯L-1 (51.33⯱â¯17.01); however, it was also not significantly different from the concentration of 20, 30 and 40â¯mgL-1. In addition, a higher hatching rate was also recorded using glutathione concentration of 50â¯mgâ¯L-1 (40.33⯱â¯12.89), this value was not significantly different from using 40â¯mgL-1 glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40â¯mgâ¯L-1 of diluent.