RESUMEN
The serological analysis of recombinant cDNA expression libraries (SEREX) by utilizing a library derived from a human pancreatic adenocarcinoma cell line and IgG antibodies from an allogeneic patient serum led to the identification of 18 genes: 13 of these were known genes, and 5 were unknown genes. In Northern and RT-PCR analyses, we found that the expression of mRNA of 14 genes was elevated in pancreatic cancer cell lines compared with the levels in normal pancreatic tissues. In addition, the expression of mRNA of hsp105 in colon cancer was greater than that in normal colon tissue. Immunohistochemical analysis using anti-hsp105 antibody revealed that an increased expression of hsp105 is a characteristic feature of pancreatic ductal and colon adenocarcinoma. Furthermore, hsp105 immunoreactivity in some cases of gastric, esophageal, and hepatocellular carcinoma was much stronger than that in normal corresponding tissues. These molecules identified may provide good diagnostic markers for cancer cells.
Asunto(s)
Antígenos de Neoplasias/genética , Neoplasias Pancreáticas/genética , Actinas/análisis , Antígenos de Neoplasias/análisis , Northern Blotting , Carcinoma Ductal Pancreático/genética , Carcinoma Ductal Pancreático/inmunología , Carcinoma Ductal Pancreático/patología , Clonación Molecular , ADN Complementario/genética , ADN Complementario/inmunología , Regulación Neoplásica de la Expresión Génica , Biblioteca de Genes , Proteínas del Choque Térmico HSP110 , Proteínas HSP70 de Choque Térmico/análisis , Proteínas HSP70 de Choque Térmico/genética , Proteínas de Choque Térmico/análisis , Humanos , Inmunohistoquímica , Queratinas/análisis , Neoplasias Pancreáticas/inmunología , Neoplasias Pancreáticas/patología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Distribución Tisular , Células Tumorales CultivadasRESUMEN
A case of intralobar pulmonary sequestration with three aberrant arteries was reported. The patient was a 26-year-old man. His chest film showed an abnormal shadow suspected of a pulmonary sequestration in the left lower lobe of the lung. Computed tomography of the chest revealed that the aberrant arteries originated from the descending aorta and circulated the emphysematous area of the left lung. Angiography clearly demonstrated three aberrant arteries and three veins draining into the hemiazygos and lower pulmonary veins. Pulmonary sequestration was diagnosed and then resected. Microscopically, the resected lung had emphysematous change, multiple cysts and winded vessels. Aberrant arteries were the elastic type of vessels and accompanied with atherosclerosis. According to the review of 139 Japanese cases of intralobar pulmonary sequestration, 7% of all patients had more than two aberrant arteries. Moreover, only 3% of patients had veins draining into the systemic vessels. In many cases the thin aberrant arteries were clearly demonstrated by angiography and the draining veins were detected. We consider that angiography is necessary to show definitely the aberrant arteries and drainage veins.
