RESUMEN
The collagen receptor DDR1 is a receptor tyrosine kinase that promotes progression of a wide range of human disorders. Little is known about how ligand binding triggers DDR1 kinase activity. We previously reported that collagen induces DDR1 activation through lateral dimer association and phosphorylation between dimers, a process that requires specific transmembrane association. Here we demonstrate ligand-induced DDR1 clustering by widefield and super-resolution imaging and provide evidence for a mechanism whereby DDR1 kinase activity is determined by its molecular density. Ligand binding resulted in initial DDR1 reorganisation into morphologically distinct clusters with unphosphorylated DDR1. Further compaction over time led to clusters with highly aggregated and phosphorylated DDR1. Ligand-induced DDR1 clustering was abolished by transmembrane mutations but did not require kinase activity. Our results significantly advance our understanding of the molecular events underpinning ligand-induced DDR1 kinase activity and provide an explanation for the unusually slow DDR1 activation kinetics.
Asunto(s)
Colágeno/metabolismo , Receptor con Dominio Discoidina 1/química , Receptor con Dominio Discoidina 1/metabolismo , Multimerización de Proteína , Colágeno/química , Receptor con Dominio Discoidina 1/genética , Células HEK293 , Humanos , Mutación , FosforilaciónRESUMEN
The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acid-soluble collagens in the form of single triple helices, which are not representative of the densely packed insoluble collagen fibers found in tissues. In this protocol, we describe a method that allows stimulating DDR1 locally with collagen-coated beads. Latex beads are first coated with acid-soluble collagen, then added to cells expressing DDR1. Recruitment of DDR1 to the beads and collagen-induced DDR1 phosphorylation is visualized by immunofluorescence microscopy on a widefield microscope. In this method, densely packed collagen is presented to cells in an insoluble form. Bead coating is easy to perform, and this method thus presents a straightforward protocol with which to study local recruitment of collagen receptors to insoluble collagen.
RESUMEN
Collagen-based scaffolds may require chemical crosslinking to achieve mechanical properties suitable for tissue engineering. Carbodiimide treatment, often used for this purpose, consumes amino acid side chains required for receptor recognition, thus reducing cell-collagen interaction. Here, we restore recognition and function of both von Willebrand Factor (VWF) and Discoidin Domain Receptor 2 (DDR2) to crosslinked collagen films by derivatisation with a specific triple-helical peptide (THP), an approach previously applied to integrin-mediated cellular adhesion. The THP contained the collagen III-derived active sequence, GPRGQOGVNleGFO, conjugated to a photoreactive moiety, diazirine, allowing UV-dependent covalent coupling to collagen films. Crosslinking of collagen films attenuated the binding of recombinant VWF A3 domain and of DDR2 (as the GST and Fc fusions, respectively), and coupling of the specific THP restored their attachment. These derivatised films supported activation of DDR2 expressed in either COS-7 or HEK293â¯cells, reflected by phosphorylation of tyrosine 740, and VWF-mediated platelet deposition from flowing blood was restored. Further, such films were able to increase low-density lipoprotein uptake in vascular endothelial cells, a marker for endothelial phenotype. Thus, covalent linkage of specific THPs to crosslinked collagen films i) restores their cognate protein binding, ii) triggers the corresponding cellular responses, and iii) demonstrates the broad applicability of the approach to a range of receptors for applications in regenerative medicine.
Asunto(s)
Materiales Biocompatibles/metabolismo , Colágeno/metabolismo , Receptor con Dominio Discoidina 2/metabolismo , Péptidos/metabolismo , Factor de von Willebrand/metabolismo , Animales , Materiales Biocompatibles/química , Células COS , Chlorocebus aethiops , Colágeno/química , Reactivos de Enlaces Cruzados/química , Reactivos de Enlaces Cruzados/metabolismo , Receptor con Dominio Discoidina 2/agonistas , Células Endoteliales/metabolismo , Células HEK293 , Humanos , Péptidos/química , Unión Proteica , Andamios del Tejido/química , Factor de von Willebrand/agonistasRESUMEN
The collagen-binding receptor tyrosine kinase DDR1 (discoidin domain receptor 1) is a drug target for a wide range of human diseases, but the molecular mechanism of DDR1 activation is poorly defined. Here we co-expressed different types of signalling-incompetent DDR1 mutants ('receiver') with functional DDR1 ('donor') and demonstrate phosphorylation of receiver DDR1 by donor DDR1 in response to collagen. Making use of enforced covalent DDR1 dimerisation, which does not affect receptor function, we show that receiver dimers are phosphorylated in trans by the donor; this process requires the kinase activity of the donor but not that of the receiver. The receiver ectodomain is not required, but phosphorylation in trans is abolished by mutation of the transmembrane domain. Finally, we show that mutant DDR1 that cannot bind collagen is recruited into DDR1 signalling clusters. Our results support an activation mechanism whereby collagen induces lateral association of DDR1 dimers and phosphorylation between dimers.