Effect of drying treatments on the global metabolome and health-related compounds in tomatoes.

Drying fruits and vegetables is a long-established preservation method, and for tomatoes, in most cases sun-drying is preferred. Semi-drying is relatively a new application aimed to preserve better the original tomato properties. We have assessed the effects of different drying methods on the phytochemical variation in tomato products using untargeted metabolomics and targeted analyses of key compounds. An LC-MS approach enabled the relative quantification of 890 mostly semi-polar secondary metabolites and GC-MS analysis in the relative quantification of 270 polar, mostly primary metabolites. Metabolite profiles of sun-dried and oven-dried samples were clearly distinct and temperature-dependent. Both treatments caused drastic changes in lycopene and vitamins with losses up to > 99% compared to freeze-dried controls. Semi-drying had less impact on these compounds. In vitro bioaccessibility analyses of total phenolic compounds and antioxidants in a gastrointestinal digestion protocol revealed the highest recovery rates in semi-dried fruits. Semi-drying is a better way of preserving tomato phytochemicals, based on both composition and bioaccessibility results.

Patulin, ergosterol and Howard mold count (HMC): Which one is the best quality indicator for tomatoes?

Ergosterol, an important constituent of fungal cell wall, was started to be used as a quality parameter for the tomatoes and derivatives instead of Howard mold count (HMC). In addition, patulin is another important microbiological quality indicator of molds such as Penicillium, Aspergillus, and Byssochlamys. In this study, it is aimed to identify the relationship between HMC, patulin, and ergosterol in juices produced from the tomatoes which has the surface rotten of 0% (sound), 25%, 50%, 75%, and 100%. As the rotten proportion increased, HMC, ergosterol, and patulin values increased. Linear correlations (r) between HMC, ergosterol, patulin, and rotten proportion were calculated as 0.99, 0.99, and 0.98, respectively. In addition, linear correlations between HMC and ergosterol (r = 0.95), HMC and patulin (r = 0.95) and ergosterol and patulin (r = 0.98) were found. In contrast, non-linear correlation between pH, brix and titratable acidity, and the rotten proportion was determined. PRACTICAL APPLICATIONS: The manuscript has provided the relationship between HMC, patulin, and ergosterol in juices produced from the tomatoes which have the surface rotten of 0% (sound), 25%, 50%, 75%, and 100%. Linear correlations between rotten proportions of tomatoes and HMC, ergosterol, patulin and between HMC and ergosterol, HMC and patulin, and ergosterol and patulin were determined in juices produced from rotten tomatoes. Patulin indicated correlation with HMC and ergosterol in juices produced from rotten tomatoes. In addition to HMC and ergosterol, patulin has great potential for the assessment of tomato products as associated with the rotten proportion.

Effects of temperature and packaging types on ergosterol and Howard mold count values of tomato paste during storage.

The objective of the present study was to investigate the effects of temperature and packaging on ergosterol and Howard mold count (HMC) changes of tomato paste during storage. The other purpose of this study was to determine whether the measurement of ergosterol stability in tomato paste can be useful for the assessment of microbiological quality of tomato paste as related to the storage temperature (4, 20, 28, or 37°C) and time. Ergosterol analysis was done by using high-performance liquid chromatography. Tomato paste samples were packaged in either aseptic bags or tin boxes and stored at 4, 20, 28, or 37°C for a period of 10 months. The detection limit of ergosterol was 0.1 mg/kg. Measurements showed that packaging and storage temperatures of 28 and 37°C have a considerable influence on ergosterol and HMC changes in the product. The poor precision of the "percentage of discarded fruits" and HMC methods has increased the importance of ergosterol for the microbiological quality evaluation of tomato and tomato products. This article reports the data from what we believe to be the first survey for the influence of storage temperature and packaging material on ergosterol and HMC changes of tomato paste during storage.

Patulin & ergosterol: new quality parameters together with aflatoxins in hazelnuts.

Hazelnuts of three different categories, mouldy, hidden mould and sound (undamaged), were investigated for their contents of aflatoxins (B1, B2, G1 and G2), patulin, and ergosterol. Samples were obtained from five hazelnut processing plants located in a major hazelnut producing area in the Black Sea region in Turkey. All aflatoxins, patulin and ergosterol were determined by high performance liquid chromatography (HPLC). Sound hazelnuts were contaminated with trace or zero amounts of aflatoxins, patulin and ergosterol, so they posed no risk for the consumer when national and/or international regulatory limits were considered. Mouldy and hidden mould hazelnuts were contaminated with high (246-510ppb; 141-422ppb) aflatoxin levels, respectively. Aflatoxin B1 content was significantly correlated with the patulin and ergosterol contents in mouldy and hidden mould hazelnuts. However, there was no significant correlation between patulin and ergosterol contents of mouldy and hidden mould hazelnuts.

A new quality parameter in tomato and tomato products: ergosterol.

The poor precision of the "percentage of discarded fruits" and "Howard mold count" methods has increased the importance of ergosterol for the microbiological quality evaluation of tomato and tomato products. Ergosterol, a constituent of the cell wall of some important vegetable parasites, such as molds, has been recently recognized as a potential objective parameter useful for the characterization of the quality of processing tomatoes. Thus, 15 mg of ergosterol/kg total solids has been stated as an acceptability maximum limit of tomato products.