RESUMEN
In the issue we demonstrate results of Epstein-Barr virus (EBV) detection by chromogenic in situ hybridization (CISH) in epithelial and lymphoid cells of the palatine tonsil in patients with chronic tonsillitis. Virus genome detections were performed using RNA-probes with digoxigenin-labeled oligonucleotides which target EBV RNA, notably RNA-transcripts of virus genomic DNA. The obtained data confirm the virus lymphotropism and also tropism to epithelial cells of both surface and cryptal epithelium of the palatine tonsil. CISH method in combination with immunohistochemical identification of virus protein products opens new possibilities for clinicopathological monitoring of the different clinical forms of the chronic tonsillitis, as well as new horizon for understanding intrinsic role of EBV in tonsillar pathology.
Asunto(s)
Infecciones por Virus de Epstein-Barr/diagnóstico , Infecciones por Virus de Epstein-Barr/metabolismo , Herpesvirus Humano 4/metabolismo , Hibridación in Situ/métodos , Tonsilitis/diagnóstico , Tonsilitis/metabolismo , Enfermedad Crónica , ADN Viral/genética , ADN Viral/metabolismo , Infecciones por Virus de Epstein-Barr/genética , Infecciones por Virus de Epstein-Barr/patología , Femenino , Herpesvirus Humano 4/genética , Humanos , Masculino , ARN Viral/genética , ARN Viral/metabolismo , Tonsilitis/genética , Tonsilitis/patología , Tonsilitis/virologíaRESUMEN
In the Large Helical Device (LHD), various spectroscopic diagnostics have been applied to study the ablation process of an advanced impurity pellet, tracer-encapsulated solid pellet (TESPEL). The total light emission from the ablation cloud of TESPEL is measured by photomultipliers equipped with individual interference filters, which provide information about the TESPEL penetration depth. The spectra emitted from the TESPEL ablation cloud are measured with a 250 mm Czerny-Turner spectrometer equipped with an intensified charge coupled device detector, which is operated in the fast kinetic mode. This diagnostic allows us to evaluate the temporal evolution of the electron density in the TESPEL ablation cloud. In order to gain information about the spatial distribution of the cloud parameters, a nine image optical system that can simultaneously acquire nine images of the TESPEL ablation cloud has recently been developed. Several images of the TESPEL ablation cloud in different spectral domains will give us the spatial distribution of the TESPEL cloud density and temperature.