[On the question of occurrence and the problem of hygiene rating of fungal air pollution of the environment of residential and public buildings].

Comprehensive sanitary examinations of fungal pollution of the environment of residential and public buildings were performed. There is established the occurrence of sensitization of the population associated with the fungal contamination of the wallings of buildings and presence of viable mold spores in the indoor air environment. Major factors determining the degree of fungal contamination of indoor environments: increasing humidity of indoor air due to leaks and bays, the area of enclosure structures and the temperature factor have been identified.

[Fungal contamination of dwelling and public buildings: hygienic aspects].

Dwelling and public buildings underwent comprehensive hygienic studies for fungal contamination. Human allergization associated with fungal contamination within the building envelopes and with the viable fungal spores in the air of enclosed spaces was found to be prevalent. The leading factors determining the extent to which the internal environment of premises was exposed to fungal contamination: their increased air humidity due to leakages and inlets, the affected area of building envelopes, and a temperature factor were revealed. The criteria showing it necessary to undertake specific measures to optimize the living conditions of the population were defined.

[The correlation between the synthesis of beta-carotene and zygote formation by Blakeslea trispora heterothallic strains].

It was demonstrated for the first time that the level of carotenogenesis by the heterothallic Blakeslea trispora strains intensively forming zygospores decreased under conditions of a surface cocultivation during their sexual interaction as compared with the strains grown separately. On the contrary, carotenogenesis was stimulated during a sexual interaction of the strains incapable of forming zygotes. In a submerged culture, the zygote-forming pairs of strains synthesized a considerably larger amount of trisporic acids but a smaller amount of carotenoids than the strains not forming zygospores. The discovered inverse dependence between zygote formation and carotenogenesis allowed us to suggest the inability to form zygotes as a criterion for selecting carotenogenic strain pairs.

[The role of dwelling environment in human health].

The authors adduce the ecologo-hygienic characterization of the most significant dwelling environmental factors, such as chemical air pollution, microclimatic parameters, biological pollution, noise, electromagnetic fields, light, etc. They have developed a conception of providing ecologo-hygienic dwelling safety.

[The experimental and clinical validation of the use of a polikatan preparation in periodontal diseases]. / Eksperimental'no-klinicheskoe obosnovanie primeneniia preparata polikatan pri zabolevaniiakh parodonta.

Polycatane, a bischofite-based drug (bischofite is a mineral containing up to 95% dry residue of MgCl2 x 6H2O), exerted antiinflammatory and bacteriostatic effects and stimulated phagocytosis and healing of burn infected ulcer in the buccal mucosa of experimental animals. The drug was effective in the treatment of patients with chronic catarrhal gingivitis and generalized periodontitis of light and medium severity. By its antiinflammatory and wound-healing effects polycatane is superior to polyminerol, the reference drug.

[Preparation of functioning recombinant (hybrid) DNA molecules in vitro (genetic engineering experiments)]. / Poluchenie funktsioniruiushchikh rekombinantov (gibridnykh) molekul DNK in vitro (éksperimenty po gennoi inzhenerii)

In this paper we describe the preparation of hybrid plasmid consisting of ColE1 DNA and DNA of R6K plasmid. ColE1 plasmid represents a circular DNA with the molecular weight of 4,2-10(6) daltons. It determines colicine synthesis E1, has relaxed control of replication and is present in the cell in several dozens copies. Transmissive plasmid B6K represents a circular DNA molecule with the molecular weight of 28-10(6) daltons. It confers the resistance to amplicillin and streptomycin and belongs to the compatibility group X. This plasmid also has relaxed control of replication (up to 30 copies per cell). Circular superhelical DNA of ColE1 was prepared after it is amplification with cloramphenicol according to Clewell by ultracentrifugation in CsCl-EtBr density gradient. The yield of superhelical ColE1 DNA in our experiments was up to 300 mg/gram cells. Circular superhelical R6K DNA was isolated from E. coli strain J53 R6K grown to stationary phase in the presence of 15 mg/ml of streptomycin. The yield of superhelical DNA was equal to about 45 mg per 1 gram cells. EcoR1 restrictase was prepared from E. coli KM182 as described by Thomas et al. From 37 grams of cells 10 ml of enzyme solution was prepared. 1 ml of this solution was able to restrict completely 2 mg of ColE1 DNA during Hu incubation for 30 degrees min at 37 degrees C. Restrictase activity was analyzed by electrophoresis of ColE1 DNA restricts in 0,7% agarose gel as described by Tanaka. DNA ligase was prepared from E. coli B. cells infected by T4 phage am81 mutant as described by Weiss. The activity of enzyme solution was equal to 90 units/ml (according to Richardson). In standard "hybridization" experiment 4 mug of ColE1 DNA and 4 mug of R6K DNA were incubated for 1 hour at 37 degrees in 0,2 ml of the solution containing 40 mM tris-HCl, pH 7,4; 10 mM MgCl2 50 mM NaCl, 10 mM mercaptoethanol and 5 mul of EcoR1 restrictase. The reaction was terminated by heating the reaction mixture to 65 degrees C for 5 min. In accordance with the results of others CoLE1 DNA gave one restrict and R6K DNA-two restricted fragments. 100 mul of the restricted mixture was chilled to 0 degrees and the following ingredients were added 10 mul of 50 mM MgCl2; 10 mul of 100 mM dithiotreitol; 10 mul of 0,5 mM ATP, 20 mul of water and 8 mul of ligase solution (90 units/mul). The mixture was incubated at 0-0,5 degrees for 53 hours, thereafter it was used for the transformation of E. coli C600 according to Tanaka et al. Several classes of hybrid molecules due to different combinations of restricted fragments of parental species were expected. These molecules had to confer the immunity to colicine E1 and to carry the defect in the gene controlling colicine synthesis since EcoR1 restrictase splits ColE1 DNA in the region of corresponding gene. In addition, certain classes of transformants with hybrid DNA molecules had to be resistant to one or both antibiotics as determined by the R6K plasmid portion...