RESUMEN
This article describes the results of systemic transplantations of cardiomyoblasts grown from autologous or allogeneic bone marrow-derived mesenchymal stem cells, in the complex therapy of the late radiation da- mage of the heart, which developed after radiation therapy in 16 female patients with Hodgkin disease (HD) or breast cancer (BC). The cell therapy drastically improved the efficacy of the drug treatment, which earlier was the only option for the therapy of the radiation damage of vital organs. The effect of such therapy was clinically observed in the patients already in the first year of observation, and consisted in the decrease of the degree of the cardiac failure severity and improvement of their quality of life in the absence of HD or BC progression.
Asunto(s)
Tratamiento Basado en Trasplante de Células y Tejidos/métodos , Lesiones Cardíacas/terapia , Trasplante de Células Madre Mesenquimatosas/métodos , Traumatismos por Radiación/terapia , Adulto , Animales , Trasplante de Médula Ósea/métodos , Neoplasias de la Mama/complicaciones , Neoplasias de la Mama/patología , Neoplasias de la Mama/radioterapia , Diferenciación Celular/efectos de la radiación , Femenino , Lesiones Cardíacas/etiología , Enfermedad de Hodgkin/complicaciones , Enfermedad de Hodgkin/patología , Enfermedad de Hodgkin/radioterapia , Humanos , Persona de Mediana Edad , Miocitos Cardíacos/citología , Miocitos Cardíacos/trasplante , Calidad de Vida , Traumatismos por Radiación/etiología , Traumatismos por Radiación/patología , Radioterapia/efectos adversos , Trasplante AutólogoRESUMEN
In vitro chemiluminescent test showed that human bone marrow mesenchymal stem cells and conditioned media dose-dependently inhibit production of reactive oxygen species by macrophages: 50% inhibition of chemiluminescence (compared to biocontrol) was observed at 1:1 mesenchymal stem cell/macrophage ratio or after addition of 20-25% conditioned media to the incubation medium. The observed mechanism of inhibition of production of reactive oxygen forms can play an essential role in the formation of local immunosuppressive microenvironment in the organism after allogenic transplantation of mesenchymal stem cells.