[Molecular properties of potato spindle tuber viroid (PSTVd) isolates from the collection of the Russian Research Institute of Phytopathology].

The complete nucleotide sequences of more than 100 isolates of PSTVd collected from locations in the territory of Russia and the former USSR have been determined. These sequences represent 42 individual sequence variants, each containing 1-10 mutations with respect to the "intermediate" or type strain of PSTVd (GenBank Acc. No. v01465). Isolates containing 2-5 mutations were the most common, and 24 sequence variants are described here for the first time. Twenty one isolates contained a mutation found only in Russian and Ukrainian isolates of PSTVd up till now; i.e., replacement of the adenine at position 121 with cytosine (A121C). Many of these isolates contained two mutations--deletion of one of the three adenine residues occupying positions 118-120 plus replacement of the adenine at position 121 with either uracil or cytosine (A120, A121U/C). Both combinations of mutations were phenotypically neutral, i.e. symptom expression in Rutgers tomato was unaffected. Phylogenetic analysis of the sequences of different PSTVd isolates presented in work together with sequences of other naturally-occurring isolates obtained from Internet databases suggesting that known PSTVd isolates may be divided into four groups: i) a group of isolates from potato, tomato and solanaceous ornamentals where the type strain of PSTVd (PSTVd.018) may be considered to represent the ancestral sequence, ii) a group of isolates from potato, tomato and solanaceous ornamentals where PSTVd.123 play the same role as PSTVd.018 for the first group, and iii) a group of potato isolates where PSTVd.125 is a possible ancestral sequence. The fourth and most divergent group of PSTVd isolates differs significantly from these first three groups. The majority of isolates in this group originate from New Zealand and Australia and infect different solanaceous hosts (tomato, pepper, cape gooseberry, potato, and others).

[Isolation and some properties of potato 0-diphenol oxidase]. / Vydelenie i nekotorye svoistva o-difenoloksidazy kartofelia.

Potato tuber o-diphenoloxidase is found to exist in heterogenous mixture of forms differing from each other in their molecular weight, electrophoretic mobility, substrate specificity. A method is proposed which permits to isolate several molecular forms of the enzyme in a relatively homogenous state. Conditions are studied, in which interconversion of o-diphenoloxidase molecular forms takes place.