RESUMEN
Since the pioneering work of F. Hofmeister, Arch. Exp. Pathol. Pharmakol., 1888, 24, 247, ion specific effects have been steadily reported in the context of colloidal or protein stabilisation in electrolyte solutions. Although the observed effects are omnipresent in chemistry and biology, their origin is still under ferocious discussion. Here, we report on ion specific effects affecting the self-assembly of amine and carboxylic acid functionalised gold nanoparticles on metal surfaces as well as in electrolyte solution as a function of the monovalent cations Li+, Na+, K+ and Cs+. Mercaptooctanoic acid and 1,8-amine-octanethiol functionalised gold nanoparticles were adsorbed on structured AuPd/Pt substrates under addition of the respective chloride salts. Furthermore, the influence of the same salts on the salt induced aggregation of these AuNP was investigated. Our results demonstrate that the assembly processes on the metal surface as well as in electrolyte solution are influenced by the addition of different cations. We attribute the observed effects to ion pairing of the functional end groups with the added cations. With these findings we introduce a new parameter to control the self-assembly of 2D AuNP arrays on solid supports or of 3D AuNP networks in solution, which could be of relevance for the fabrication of new tailor-made functional materials or for biomedical applications.
RESUMEN
Gold nanoparticles (AuNPs) are versatile nanomaterials which are frequently used to manipulate and study cellular behavior on the nanometer scale. However, it has been recognized that freely diffusing colloidal particles can possess severe cytotoxicity. One strategy to overcome this harmful side effect is to tether the nanoparticles to sample surfaces. In this study, the cytotoxicity of immobilized AuNPs is investigated as a function of their sizes, surface density, and binding strength. The AuNPs are modified with positively charged aminoalkyl thiol molecules to promote the cell adhesion, while their background is passivated. Primary cortical neurons are cultured on the particle modified samples and the survival of the cells is investigated. This study reveals that besides the particle size, the particle binding strength influences the cytotoxicity during long time culture. Weakly bound particles dramatically decrease the survival of neurons while strongly bound particles hardly harm the neurons. More importantly, it is found that weakly bound AuNPs can cause higher cytotoxic effects on cells than colloidal particles dispersed in the culture medium. These results propose that the toxic effect of surface bound particles can be severe and therefore requires consideration, if nanoparticle modified surfaces are used for cell experiments.
Asunto(s)
Oro/toxicidad , Nanopartículas del Metal/toxicidad , Neuronas/efectos de los fármacos , Animales , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Oro/análisis , Oro/metabolismo , Nanopartículas del Metal/análisis , Neuronas/citología , Neuronas/metabolismo , Tamaño de la Partícula , Ratas , Propiedades de SuperficieRESUMEN
Chemical templates for the patterned immobilization of gold nanoparticles were fabricated by soft UV nanoimprint lithography. The template structures were fabricated by means of the consecutively performed process steps of nanoimprint lithography, reactive ion etching, chemical functionalization with amino groups, and lift-off of imprint resist. These chemical templates were used for the defined assembly of 20 nm diameter citrate stabilized gold nanoparticles from aqueous solution. By reducing the ionic strength of the solution, one- and zero-dimensional particle assemblies were generated on sub-100-nm template structures. By this means, the pattern resolution predefined by the lithography process could be easily enhanced by dilution of the nanoparticle solution.
RESUMEN
Alterations of the nitric oxide receptor, soluble guanylate cyclase (sGC) may contribute to the pathophysiology of pulmonary arterial hypertension (PAH). In the present study, the expression of sGC in explanted lung tissue of PAH patients was studied and the effects of the sGC stimulator BAY 63-2521 on enzyme activity, and haemodynamics and vascular remodelling were investigated in two independent animal models of PAH. Strong upregulation of sGC in pulmonary arterial vessels in the idiopathic PAH lungs compared with healthy donor lungs was demonstrated by immunohistochemistry. Upregulation of sGC was detected, similarly to humans, in the structurally remodelled smooth muscle layer in chronic hypoxic mouse lungs and lungs from monocrotaline (MCT)-injected rats. BAY 63-2521 is a novel, orally available compound that directly stimulates sGC and sensitises it to its physiological stimulator, nitric oxide. Chronic treatment of hypoxic mice and MCT-injected rats, with fully established PAH, with BAY 63-2521 (10 mg x kg(-1) x day(-1)) partially reversed the PAH, the right heart hypertrophy and the structural remodelling of the lung vasculature. Upregulation of soluble guanylate cyclase in pulmonary arterial smooth muscle cells was noted in human idiopathic pulmonary arterial hypertension lungs and lungs from animal models of pulmonary arterial hypertension. Stimulation of soluble guanylate cyclase reversed right heart hypertrophy and structural lung vascular remodelling. Soluble guanylate cyclase may thus offer a new target for therapeutic intervention in pulmonary arterial hypertension.