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Microalgae have the potential to exceed current nutrient recovery limits from wastewater, enabling water resource recovery facilities (WRRFs) to achieve increasingly stringent effluent permits. The use of photobioreactors (PBRs) and the separation of hydraulic retention and solids residence time (HRT/SRT) further enables increased biomass in a reduced physical footprint while allowing operational parameters (e.g., SRT) to select for desired functional communities. However, as algal technology transitions to full-scale, there is a need to understand the effect of operational and environmental parameters on complex microbial dynamics among mixotrophic microalgae, bacterial groups, and pests (i.e., grazers and pathogens) and to implement robust process controls for stable long-term performance. Here, we examine a full-scale, intensive WRRF utilizing mixed microalgae for tertiary treatment in the US (EcoRecover, Clearas Water Recovery Inc.) during a nine-month monitoring campaign. We investigated the temporal variations in microbial community structure (18S and 16S rRNA genes), which revealed that stable system performance of the EcoRecover system was marked by a low-diversity microalgal community (DINVSIMPSON = 2.01) dominated by Scenedesmus sp. (MRA = 55 %-80 %) that achieved strict nutrient removal (effluent TP < 0.04 mg·L-1) and steady biomass concentration (TSSmonthly avg. = 400-700 mg·L-1). Operational variables including pH, alkalinity, and influent ammonium (NH4+), correlated positively (p < 0.05, method = Spearman) with algal community during stable performance. Further, the use of these parameters as operational controls along with N/P loading and SRT allowed for system recovery following upset events. Importantly, the presence or absence of bacterial nitrification did not directly impact algal system performance and overall nutrient recovery, but partial nitrification (potentially resulting from NO2- accumulation) inhibited algal growth and should be considered during long-term operation. The microalgal communities were also adversely affected by zooplankton grazers (ciliates, rotifers) and fungal parasites (Aphelidium), particularly during periods of upset when algal cultures were experiencing culture turnover or stress conditions (e.g., nitrogen limitation, elevated temperature). Overall, the active management of system operation in order to maintain healthy algal cultures and high biomass productivity can result in significant periods (>4 months) of stable system performance that achieve robust nutrient recovery, even in winter months in northern latitudes (WI, USA).
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Microalgas , Aguas Residuales , Biomasa , Eliminación de Residuos Líquidos/métodosRESUMEN
Mixed community microalgal wastewater treatment technologies have the potential to advance the limits of technology for biological nutrient recovery while producing a renewable carbon feedstock, but a deeper understanding of their performance is required for system optimization and control. In this study, we characterized the performance of a 568 m3·day-1 Clearas EcoRecover system for tertiary phosphorus removal (and recovery as biomass) at an operating water resource recovery facility (WRRF). The process consists of a (dark) mix tank, photobioreactors (PBRs), and a membrane tank with ultrafiltration membranes for the separation of hydraulic and solids residence times. Through continuous online monitoring, long-term on-site monitoring, and on-site batch experiments, we demonstrate (i) the importance of carbohydrate storage in PBRs to support phosphorus uptake under dark conditions in the mix tank and (ii) the potential for polyphosphate accumulation in the mixed algal communities. Over a 3-month winter period with limited outside influences (e.g., no major upstream process changes), the effluent total phosphorus (TP) concentration was 0.03 ± 0.03 mg-P·L-1 (0.01 ± 0.02 mg-P·L-1 orthophosphate). Core microbial community taxa included Chlorella spp., Scenedesmus spp., and Monoraphidium spp., and key indicators of stable performance included near-neutral pH, sufficient alkalinity, and a diel rhythm in dissolved oxygen.
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Microalgas , Fósforo , Aguas Residuales , Microalgas/metabolismo , Aguas Residuales/química , Eliminación de Residuos Líquidos/métodos , Biomasa , Purificación del Agua/métodosRESUMEN
Hyperuricemia, a condition characterized by elevated levels of uric acid in the blood, is known as a risk factor for gout disease. In this study, we isolated a total of 72 MRS-grown colonies and evaluated their purine nucleosidase (PNase) activity. Among the isolated bacteria, Levilactobacillus (L.) brevis LAB42 displayed the highest PNase activity. Our findings also indicate that PNase activity can vary among lactic acid bacterial strains and during different growth phases. Based on the kinetics study, LAB42 consistently exhibits the highest PNase activity. Due to its ability to attach to Caco-2 cells and its resistance to acidic environments and bile exposure, L. brevis LAB42 was chosen for further studies and showed that with the right combination of additives, it has the potential to be an appropriate starter for milk fermentation.
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Sn-based perovskite light-emitting diodes (PeLEDs) have emerged as promising alternatives to Pb-based PeLEDs with their rapid increase in performance owing to the various research studies on inhibiting Sn oxidation. However, the absence of defect passivation strategies for Sn-based perovskite LEDs necessitates further research in this field. We performed systematic studies to investigate the design rules for defect passivation agents for Sn-based perovskites by incorporating alkali/multivalent metal salts with various cations and anions. From the computational and experimental analyses, sodium trifluoromethanesulfonate (NaTFMS) was found to be the most effective passivation agent for PEA2SnI4 films among the explored candidate agents owing to favorable reaction energetics to passivate iodide Frenkel defects. Consequently, the incorporation of NaTFMS facilitates the formation of uniform films with relatively large crystals and reduced Sn4+. The NaTFMS-containing PEA2SnI4 PeLEDs demonstrate an improved luminance of 138.9 cd/m2 and external quantum efficiency (EQE) of 0.39% with an improved half-lifetime of more than threefold. This work provides important insight into the design of defect passivation agents for Sn-based perovskites.
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Recently, as new threats from attackers are discovered, the damage and scale of these threats are increasing. Vulnerabilities should be identified early, and countermeasures should be implemented to solve this problem. However, there are limitations to applying the vulnerability discovery framework used in practice. Existing frameworks have limitations in terms of the analysis target. If the analysis target is abstract, it cannot be easily applied to the framework. Therefore, this study proposes a framework for vulnerability discovery and countermeasures that can be applied to any analysis target. The proposed framework includes a structural analysis to discover vulnerabilities from a scenario composition, including analysis targets. In addition, a proof of concept is conducted to derive and verify threats that can actually occur through threat modeling. In this study, the open platform communication integrated architecture used in the industrial control system and industrial Internet of Things environment was selected as an analysis target. We find 30 major threats and four vulnerabilities based on the proposed framework. As a result, the validity of malicious client attacks using certificates and DoS attack scenarios using flooding were validated, and we create countermeasures for these vulnerabilities.
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Redes de Comunicación de Computadores , Internet de las Cosas , HumanosRESUMEN
In this study, the Search Your Mind (S.Y.M., ) project aimed to collect prospective digital phenotypic data centered on mood and anxiety symptoms across psychiatric disorders through a smartphone application (app) platform while using both centralized and decentralized research designs: the centralized research design is a hybrid of a general prospective observational study and a digital platform-based study, and it includes face-to-face research such as informed written consent, clinical evaluation, and blood sampling. It also includes digital phenotypic assessment through an application-based platform using wearable devices. Meanwhile, the decentralized research design is a non-face-to-face study in which anonymous participants agree to electronic informed consent forms on the app. It also exclusively uses an application-based platform to acquire individualized digital phenotypic data. We expect to collect clinical, biological, and digital phenotypic data centered on mood and anxiety symptoms, and we propose a possible model of centralized and decentralized research design.
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Among various plastic additives, di-2-ethylhexyl phthalate (DEHP) has been a great concern due to its high leaching potential and harmful effects on both human and the ecosystem. For the effective oxidation and mineralization of DEHP by ozone in the existing TiO2 catalytic processes, the heterogeneous catalyst, vanadium oxide (V2O5)-incorporated TiO2 (V2O5/TiO2), was synthesized. The generation of hydroxyl radicals was promoted by cyclic redox reactions of vanadium atoms in V2O5/TiO2 via the increase of surface oxygen vacancies by the replacement of V5+ species in the lattice of TiO2. The catalytic ozonation in the presence of V2O5/TiO2 exhibited the significantly higher degradation of DEHP with the pseudo-second-order kinetic constant of 1.7 × 105 mM-1min-1 and the removal efficiency of 58.7% after 60 s in 2 mg/L of ozone. The degradation of DEHP was initiated by the shortening of the alkyl-side chain followed by the opening of esterified benzene moieties.
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Dietilhexil Ftalato , Nanopartículas , Ozono , Catálisis , Ecosistema , Humanos , Óxidos/química , Ozono/química , Ácidos Ftálicos , Titanio/química , Vanadio/químicaRESUMEN
Recently, the number of users and the demand for live-streaming services have increased. This has exponentially increased the traffic to such services, and live-streaming service platforms in Korea use a grid computing system that distributes traffic to users and reduces traffic loads. However, ensuring security with a grid computing system is difficult because the system exchanges general user traffic in a peer-to-peer (P2P) manner instead of receiving data from an authenticated server. Therefore, in this study, to explore the vulnerabilities of a grid computing system, we investigated a vulnerability discovery framework that involves a three-step analysis process and eight detailed activities. Four types of zero-day vulnerabilities, namely video stealing, information disclosure, denial of service, and remote code execution, were derived by analyzing a live-streaming platform in Korea, as a representative service, using grid computing.
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Sistemas de Computación , Computadores , República de CoreaRESUMEN
A series of PROTACs (PROteolysis-TArgeting Chimeras) consisting of bicalutamide analogs and thalidomides were designed, synthesized, and biologically evaluated as novel androgen receptor (AR) degraders. In particular, we found that PROTAC compound 13b could successfully demonstrate a targeted degradation of AR in AR-positive cancer cells and might be a useful chemical probe for the investigation of AR-dependent cancer cells, as well as a potential therapeutic candidate for prostate cancers.
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Antagonistas de Andrógenos/química , Anilidas/química , Nitrilos/química , Receptores Androgénicos/química , Talidomida/química , Compuestos de Tosilo/química , Antagonistas de Andrógenos/síntesis química , Antagonistas de Andrógenos/farmacología , Anilidas/farmacología , Sitios de Unión , Línea Celular , Técnicas de Química Sintética , Humanos , Modelos Biológicos , Modelos Moleculares , Conformación Molecular , Estructura Molecular , Nitrilos/farmacología , Unión Proteica , Proteolisis/efectos de los fármacos , Receptores Androgénicos/metabolismo , Relación Estructura-Actividad , Talidomida/farmacología , Compuestos de Tosilo/farmacologíaRESUMEN
Microalgae separation technology is essential for both executing laboratory-based fundamental studies and ensuring the quality of the final algal products. However, the conventional microalgae separation technology of micropipetting requires highly skilled operators and several months of repeated separation to obtain a microalgal single strain. This study therefore aimed at utilizing microfluidic cell sorting technology for the simple and effective separation of microalgae. Microalgae are characterized by their various morphologies with a wide range of sizes. In this study, a contraction-expansion array microchannel, which utilizes these unique properties of microalgae, was specifically employed for the size-based separation of microalgae. At Reynolds number of 9, two model algal cells, Chlorella vulgaris (C. vulgaris) and Haematococcus pluvialis (H. pluvialis), were successfully separated without showing any sign of cell damage, yielding a purity of 97.9% for C. vulgaris and 94.9% for H. pluvialis. The result supported that the inertia-based separation technology could be a powerful alternative to the labor-intensive and time-consuming conventional microalgae separation technologies.
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Pseudomonas aeruginosa-encapsulated alginate/gellan gum microbeads (PAGMs) were prepared at the condition of 10 g/L alginate, 1 g/L gellan gum, and 2.57 mM calcium ions, and investigated for the biodegradation of a diesel-contaminated groundwater. The degradation of diesel with PAGMs reached 71.2% after 10days in the aerobic condition, while that of suspended bacteria was only 32.0% even after 30days. The kinetic analysis showed that PAGMs had more than two-order higher second-order kinetic constant than that of the suspended bacteria. Interestingly, the degradation of diesel was ceased due to the depletion of the dissolved oxygen after 10 day in the PAGM reactor, but the microbial degradation activity was immediately restored after the addition of oxygen to 10.5 mg/L. The change in ATP concentration and the viability of bacteria showed that the microbial activity in PAGMs were maintained (66.4%, and 84.3%, respectively) even after 30days of experiment with PAGMs due to the protective barrier of the microbeads, whereas those of suspended bacteria showed significant decrease to 6.2% and 14.4% of initial value, respectively, due to the direct contact to toxic hydrocarbons. The results suggested that encapsulation of bacterial cells could be used for the enhanced biodegradation of diesel hydrocarbons in aqueous systems.
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Alginatos , Pseudomonas aeruginosa , Biodegradación Ambiental , Hidrocarburos , Cinética , Microesferas , Polisacáridos BacterianosRESUMEN
Microalgal photosynthesis is a promising solar energy conversion process to produce high concentration biomass, which can be utilized in the various fields including bioenergy, food resources, and medicine. In this research, we study the optical design rule for microalgal cultivation systems, to efficiently utilize the solar energy and improve the photosynthesis efficiency. First, an organic luminescent dye of 3,6-Bis(4'-(diphenylamino)-1,1'-biphenyl-4-yl)-2,5-dihexyl-2,5-dihydropyrrolo3,4-c pyrrole -1,4-dione (D1) was coated on a photobioreactor (PBR) for microalgal cultivation. Unlike previous reports, there was no enhancement in the biomass productivities under artificial solar illuminations of 0.2 and 0.6 sun. We analyze the limitations and future design principles of the PBRs using photoluminescence under strong illumination. Second, as a multiple-bandgaps-scheme to maximize the conversion efficiency of solar energy, we propose a dual-energy generator that combines microalgal cultivation with spectrally selective photovoltaic cells (PVs). In the proposed system, the blue and green photons, of which high energy is not efficiently utilized in photosynthesis, are absorbed by a large-bandgap PV, generating electricity with a high open-circuit voltage (Voc) in reward for narrowing the absorption spectrum. Then, the unabsorbed red photons are guided into PBR and utilized for photosynthesis with high efficiency. Under an illumination of 7.2 kWh m-2 d-1, we experimentally verified that our dual-energy generator with C60-based PV can simultaneously produce 20.3 g m-2 d-1 of biomass and 220 Wh m-2 d-1 of electricity by utilizing multiple bandgaps in a single system.
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Microalgas/fisiología , Fotosíntesis , Energía Solar , Reactores Biológicos/microbiología , LuminiscenciaRESUMEN
During the manufacture of H1N1 microneedles, a stabilizer is usually added to maintain the antigenicity of the vaccine. However, finding a suitable stabilizer is difficult, and the addition of a stabilizer can limit the antigen dose and the addition of an adjuvant because of the limited volume of the microneedles. In this study, the authors evaluated whether H1N1 microneedles could be fabricated without a stabilizer by keeping the production environment at a low temperature. H1N1 microneedle patches without a stabilizer were prepared in a process that involved maintaining a low temperature of 10⯰C. The protective immune response to this method of drug application was investigated by comparing it with traditional intramuscular (IM) immunization and with the use of H1N1 microneedles with a stabilizer. A process-sensitive antigen, H1N1, was stabilized without the use of a stabilizer in a process that maintained a low temperature of 10⯰C. The preparation process consisted of coating and drying processes. In animal experiments, mice were immunized using an array of low-temperature H1N1 microneedles without a stabilizer (LT-MN), and they showed strong antibody responses. Compared to three other application methods of traditional IM immunization, low-temperature H1N1 microneedles with a stabilizer (LT-MN-T), and room-temperature H1N1 microneedles with a stabilizer (RT-MN-T), LT-MN produced comparable results in inducing protective immunity. A plaque reduction neutralization test found that LT-MN and LT-MN-T provided greater immunity compared with IM and RT-MN-T. A process in which the temperature is maintained at 10⯰C can provide successful vaccination with H1N1 microneedles without the addition of a stabilizer. This process can be applied to various temperature-sensitive biologics.
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Excipientes/química , Subtipo H1N1 del Virus de la Influenza A/inmunología , Vacunas contra la Influenza/química , Animales , Anticuerpos Antivirales/inmunología , Formación de Anticuerpos/inmunología , Femenino , Inmunización/métodos , Vacunas contra la Influenza/inmunología , Inyecciones Intradérmicas/métodos , Ratones , Ratones Endogámicos BALB C , Agujas , Pruebas de Neutralización/métodos , Temperatura , Vacunación/métodosRESUMEN
A process to produce both biodiesel and alginate in an integrated manner from a brown seaweed, Laminaria japonica, was established. Mannitol, a major carbon constituent in L. japonica, served to produce neutral lipids via the heterotrophic cultivation of an oleaginous yeast, Cryptococcus sp.; and simultaneously alginate, a high value product, was extracted to enhance the economic feasibility of the entire process. Only autoclave pretreatment, without need of any chemical agents, was enough to recover all the essential nutrients for the yeast cultivation. Specifically, it could recover 6.4â¯gâ¯L-1 of mannitol to a degree comparable to 6.6â¯gâ¯L-1 obtained by acid-aided pretreatment using 1.5% (v/v) of H2SO4. Maximum fatty acids methyl esters (FAME) content was 30.37% with FAME productivity of 0.56â¯gâ¯L-1â¯d-1, and the produced FAME could meet the biodiesel quality standards. Na2CO3-based method showed the best efficiency of alginate recovery, yielding 21.06% (w/w). This study supports that L. japonica can indeed be a promising low-cost feedstock for biodiesel production, and it is more so when a high-value product alginate is co-produced.
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Alginatos/metabolismo , Biocombustibles , Laminaria/fisiología , Cryptococcus/fisiología , Algas Marinas/fisiologíaRESUMEN
BACKGROUND: Active restraint for the elbow joint is provided by the soft tissue component, which consists of a musculoligamentous complex. A lesion of the lateral collateral ligament complex (LCLC) is thought to be the primary cause of posterolateral rotatory instability in the elbow. Its role as a protective reflexogenic structure is supported by the existence of ultrastructural mechanoreceptors. The aim of this study was to describe the existence and distribution of LCLC mechanoreceptors in the human elbow joint and to determine their role in providing joint stability. METHODS: Eight LCLCs were harvested from fresh frozen cadaver elbows. Specimens were carefully separated from the lateral epicondyle and ulna. The ligament complex was divided into 7 regions of interest and stained with modified gold chloride. Microscopic evaluation was performed for Golgi, Ruffini, and Pacinian corpuscles. The number, distribution, and density of each structure were recorded. RESULTS: Golgi, Ruffini, and Pacinian corpuscles were observed in LCLCs, with variable distribution in each region of interest. Ruffini corpuscles showed the highest total mechanoreceptor density. Mechanoreceptor density was higher at bony attachment sites. CONCLUSION: The existence and role of each mechanoreceptor defined the purpose of each region of interest. Mechanoreceptors are beneficial for its proprioceptive feature towards a successful elbow ligament reconstruction.
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Type 2 diabetes is characterized by insulin resistance, which leads to increased blood glucose levels. Adipocytes are involved in the development of insulin resistance, resulting from the dysfunction of the insulin signaling pathway. In this study, we investigated whether meso-dihydroguaiaretic acid (MDGA) may modulate glucose uptake in adipocytes, and examined its mechanism of action. MDGA enhanced adipogenesis through up-regulation of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α in 3T3-L1 adipocytes partially differentiated with sub-optimal concentrations of insulin. MDGA also increased glucose uptake by stimulating expression and translocation of glucose transporter 4 (GLUT4) in adipocytes. These results suggest that MDGA may increase GLUT4 expression and its translocation by promoting insulin sensitivity, leading to enhanced glucose uptake.
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Adipocitos/citología , Transportador de Glucosa de Tipo 4/metabolismo , Glucosa/metabolismo , Guayacol/análogos & derivados , Lignanos/farmacología , Células 3T3-L1 , Adipocitos/efectos de los fármacos , Adipocitos/metabolismo , Adipogénesis , Animales , Guayacol/farmacología , Ratones , PPAR gamma/metabolismo , Regulación hacia ArribaRESUMEN
In this study, bicarbonate was proposed as an alternative carbon source to overcome exceedingly low CO2 fixation efficiency of conventional microalgae cultivation system. 5gL-1 of sodium bicarbonate was found to well support the growth of Dunaliella salina, showing 2.84-fold higher specific growth rate than a bicarbonate-free control. This bicarbonate-fed cultivation also could yield biomass productivity similar to that of CO2-based system as long as pH was controlled. While the supplied CO2, because of its being a gas, was mostly lost and only 3.59% of it was used for biomass synthesis, bicarbonate was effectively incorporated into the biomass with 91.40% of carbon utilization efficiency. This study showed that the bicarbonate-based microalgae cultivation is indeed possible, and can even become a truly environment-friendly and workable approach, provided that a CO2 mineralization technology is concomitantly established.
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Bicarbonatos , Dióxido de Carbono , Chlorophyta , Carbono , MicroalgasRESUMEN
BACKGROUND: Range of motion (ROM) is a clinically important parameter in evaluating joint function. However, dynamic evaluation to determine the quality of the arm motion using digitized measurement is often overlooked during clinical assessment. We evaluated the accuracy of Kinect v2 (Microsoft, Redmond, WA, USA) as a digital tool for measuring shoulder ROM objectively and proposed a concept of motion smoothness reflecting the quality of arm motion. METHODS: Ten male participants were included in a 2-stage experiment. First, shoulder ROM was measured in 4 static poses (flexion, abduction, external rotation, and internal rotation) with Kinect v2, a 3-dimensional (3D) motion analysis system, and goniometry. Second, participants performed a point-to-point arm motion as naturally as possible. Kinematic data were collected with Kinect v2 and the 3D motion analysis system and then postprocessed to acquire parameters related to motion smoothness, including peak to mean velocity ratio, acceleration to movement time ratio, and number of peaks. RESULTS: Kinect v2 resulted in very good agreement of ROM measurement (r > 0.9) with the 3D motion analysis (95% limits of agreement < ±8°) compared with goniometry (95% limits of agreement < ±10°). Kinect v2 also showed a good correlation and agreement of measurement of motion quality parameters compared with the 3D motion analysis (peak to mean velocity ratio, acceleration to movement time ratio, and number of peaks: r = 0.769, discrepancy = ±0.1; r = 0.922, discrepancy = ±5%; and mean = 1 ± 0, respectively). CONCLUSIONS: We show that Kinect v2 can be used as a reliable tool to measure shoulder ROM and arm motion smoothness.
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Artrometría Articular/instrumentación , Rango del Movimiento Articular/fisiología , Articulación del Hombro/fisiología , Programas Informáticos , Adulto , Artrometría Articular/métodos , Fenómenos Biomecánicos/fisiología , Humanos , MasculinoRESUMEN
This study investigated if an effluent from anaerobic digestion (AD) system can be used as a nutrients source for the microalgae cultivation, and in so doing, if the effluent can be properly treated. Nitrogen and phosphorus in the AD effluent well supported microalgal growth, and their removal efficiency reached >97.9% and 99.2%, respectively. Among four different algal species tested, Micractinium inermum particularly stood out, showing the highest biomass and FAME productivity: 0.16gL-1d-1 with 3.23gL-1 of dry cell weight, and 0.04gL-1d-1 with 27.54% (w/w) of FAME contents, respectively. As the concentrations of the nutrients decreased over time, the FAME contents were increased and its quality as well, satisfying several biodiesel quality standards. This study supports that the AD effluent can indeed serve as a cheap and nutrient-rich medium for microalgae cultivation, and equally importantly, microalgae can be a workable treatment option for it.
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Biocombustibles , Microalgas/crecimiento & desarrollo , Eliminación de Residuos Líquidos/métodos , Anaerobiosis , Biomasa , Chlorophyta/crecimiento & desarrollo , Chlorophyta/metabolismo , Microalgas/metabolismo , Nitrógeno/metabolismo , Fósforo/metabolismo , Especificidad de la Especie , Aguas Residuales/químicaRESUMEN
In this study, a microalgae-based technology was employed to treat wastewater and produce biodiesel at the same time. A local isolate Scenedesmus sp. was found to be a well suited species, particularly for an effluent from anaerobic digester (AD) containing low carbon but high nutrients (NH3-N=273mgL(-1), total P=58.75mgL(-1)). This algae-based treatment was quite effective: nutrient removal efficiencies were over 99.19% for nitrogen and 98.01% for phosphorus. Regarding the biodiesel production, FAME contents of Scenedesmus sp. were found to be relatively low (8.74% (w/w)), but overall FAME productivity was comparatively high (0.03gL(-1)d(-1)) due to its high biomass productivity (0.37gL(-1)d(-1)). FAMEs were satisfactory to the several standards for the biodiesel quality. The Scenedesmus-based technology may serve as a promising option for the treatment of nutrient-rich wastewater and especially so for the AD effluent.