RESUMEN
Trace eyeblink conditioning (TEBC) has been widely used to study associative learning in both animals and humans. In this paradigm, conditioned responses (CRs) to conditioned stimuli (CS) serve as a measure for retrieving learned associations between the CS and the unconditioned stimuli (US) within a trial. Memory consolidation, that is, learning over time, can be quantified as an increase in the proportion of CRs across training sessions. However, how hippocampal oscillations differentiate between successful memory retrieval within a session and consolidation across TEBC training sessions remains unknown. To address this question, we recorded local field potentials (LFPs) from the rat dorsal hippocampus during TEBC and investigated hippocampal oscillation dynamics associated with these two functions. We show that transient broadband responses to the CS were correlated with memory consolidation, as indexed by an increase in CRs across TEBC sessions. In contrast, induced alpha (8-10â Hz) and beta (16-20â Hz) band responses were correlated with the successful retrieval of the CS-US association within a session, as indexed by the difference in trials with and without CR.
Asunto(s)
Condicionamiento Palpebral , Hipocampo , Consolidación de la Memoria , Recuerdo Mental , Ratas Long-Evans , Hipocampo/fisiología , Masculino , Condicionamiento Palpebral/fisiología , Animales , Consolidación de la Memoria/fisiología , Recuerdo Mental/fisiología , Aprendizaje por Asociación/fisiología , Ratas , Condicionamiento Clásico/fisiología , Parpadeo/fisiologíaRESUMEN
The microvascular inflow tract, comprising the penetrating arterioles, precapillary sphincters and first-order capillaries, is the bottleneck for brain blood flow and energy supply. Exactly how aging alters the structure and function of the microvascular inflow tract remains unclear. By in vivo four-dimensional two-photon imaging, we reveal an age-dependent decrease in vaso-responsivity accompanied by a decrease in vessel density close to the arterioles and loss of vascular mural cell processes, although the number of mural cell somas and their alpha smooth muscle actin density were preserved. The age-related reduction in vascular reactivity was mostly pronounced at precapillary sphincters, highlighting their crucial role in capillary blood flow regulation. Mathematical modeling revealed impaired pressure and flow control in aged mice during vasoconstriction. Interventions that preserve dynamics of cerebral blood vessels may ameliorate age-related decreases in blood flow and prevent brain frailty.
Asunto(s)
Capilares , Pericitos , Ratones , Animales , Pericitos/fisiología , Capilares/fisiología , Arteriolas/fisiología , Encéfalo/irrigación sanguínea , HemodinámicaRESUMEN
Micro-coil magnetic stimulation of brain tissue presents new challenges for MEMS micro-coil probe fabrication. The main challenges are threefold; (i) low coil resistance for high power efficiency, (ii) low leak current from the probe into the in vitro experimental set-up, (iii) adaptive MEMS process technology because of the dynamic research area, which requires agile design changes. Taking on these challenges, we present a MEMS fabrication process that has three main features; (i) multilayer resist lift-off process to pattern up to 1800-nm-thick metal films, and special care is taken to obtain high conductivity thin-films by physical vapor deposition, and (ii) all micro-coil Al wires are encapsulated in at least 200 nm of ALD alumina and 6-µm-thick parylene C such the leak resistance is high (>210 GΩ), (iii) combining a multi-step DRIE process and maskless photolithography for adaptive design and device fabrication. The entire process requires four lithography steps. Because we avoided SOI wafers and lithography mask fabrication, the design-to-device time is shortened significantly. The resulting probes are 4-mm-long, 60-µm-thick, and down to 150 µm-wide. Selected MEMS coil devices were validated in vivo using mice and compared to previous work.
Asunto(s)
Técnicas Biosensibles , Sistemas Microelectromecánicos , Animales , Ratones , Metales , Encéfalo , Conductividad EléctricaRESUMEN
Stimulation and functional imaging studies have revealed the existence of a large network of cortical regions involved in the regulation of heart rate. However, very little is known about the link between cortical neural firing and cardiac-cycle duration (CCD). Here, we analyze single-unit and multiunit data obtained in humans at rest, and show that firing rate covaries with CCD in 16.7% of the sample (25 of 150). The link between firing rate and CCD was most prevalent in the anterior medial temporal lobe (entorhinal and perirhinal cortices, anterior hippocampus, and amygdala), where 36% (18 of 50) of the units show the effect, and to a lesser extent in the mid-to-anterior cingulate cortex (11.1%, 5 of 45). The variance in firing rate explained by CCD ranged from 0.5 to 11%. Several lines of analysis indicate that neural firing influences CCD, rather than the other way around, and that neural firing affects CCD through vagally mediated mechanisms in most cases. These results show that part of the spontaneous fluctuations in firing rate can be attributed to the cortical control of the cardiac cycle. The fine tuning of the regulation of CCD represents a novel physiological factor accounting for spontaneous variance in firing rate. It remains to be determined whether the "noise" introduced in firing rate by the regulation of CCD is detrimental or beneficial to the cognitive information processing carried out in the parahippocampal and cingulate regions.SIGNIFICANCE STATEMENT Fluctuations in heart rate are known to be under the control of cortical structures, but spontaneous fluctuations in cortical firing rate, or "noise," have seldom been related to heart rate. Here, we analyze unit activity in humans at rest and show that spontaneous fluctuations in neural firing in the medial temporal lobe, as well as in the mid-to-anterior cingulate cortex, influence heart rate. This phenomenon was particularly pronounced in the entorhinal and perirhinal cortices, where it could be observed in one of three neurons. Our results show that part of spontaneous firing rate variability in regions best known for their cognitive role in spatial navigation and memory corresponds to precise physiological regulations.
Asunto(s)
Potenciales de Acción/fisiología , Giro del Cíngulo/fisiología , Frecuencia Cardíaca/fisiología , Neuronas/fisiología , Giro Parahipocampal/fisiología , Descanso/fisiología , Adulto , Epilepsia Refractaria/diagnóstico , Epilepsia Refractaria/fisiopatología , Electrocardiografía/métodos , Femenino , Giro del Cíngulo/citología , Humanos , Masculino , Giro Parahipocampal/citologíaRESUMEN
Cellulose nanocrystals (CNC) are sustainable nanomaterials that possess high tensile strength, stiffness and surface functional groups suitable for various types of modifications. In this study, phosphorylated cellulose nanocrystals (P-CNC) were prepared via acid hydrolysis with phosphoric acid to decorate phosphate groups on the surface of CNC. Also, chitosan was modified with glycidyltrimethylammonium chloride (GTMAC) to improve its solubility. GTMAC-Chitosan (GCh) and phosphorylated cellulose nanocrystals (P-CNC) were complexed via ionic gelation to produce GCh-P-CNC nanoparticles under mild sonication. Although sodium tripolyphosphate (TPP) is a common cross-linking agent used with chitosan, its application is compromised by its metastable structure that resulted in the rapid release of its encapsulated compound. Therefore, phosphorylated cellulose nanocrystal was developed as a "nanoparticle" cross-linker and a Pickering emulsifier. The nanocomplexes transformed from a rod-like to hard sphere and random coil morphology with increasing ratio of GCh/P-CNC. In comparison with TPP-Chitosan emulsion, Pickering emulsion prepared using GCh-P-CNC complex was more stable over 3 months without coalescence and phase separation.
RESUMEN
When a saccadic eye movement is made toward a visual stimulus, the variability in accompanying primary visual cortex (V1) activity is related to saccade latency in both humans and simians. To understand the nature of this relationship, we examined the functional link between V1 activity and the initiation of visually guided saccades during the gap saccade task, in which a brief temporal gap is inserted between the turning off of a fixation stimulus and the appearance of a saccadic target. The insertion of such a gap robustly reduces saccade latency and facilitates the occurrence of extremely short-latency (express) saccades. Here we recorded single-cell activity from macaque V1 while monkeys performed the gap saccade task. In parallel with the gap effect on saccade latency the neural latency (time of first spike) of V1 response elicited by the saccade target became shorter, and the firing rate increased as the gap duration increased. Similarly, neural latency was shorter and firing rate was higher before express saccades relative to regular-latency saccades. In addition to these posttarget changes, the level of spontaneous spike activity during the pretarget period was negatively correlated with both neural and saccade latencies. These results demonstrate that V1 activity correlates with the gap effect and indicate that trial-to-trial variability in the state of V1 accompanies the variability of neural and behavioral latencies.NEW & NOTEWORTHY The link between neural activity in monkey primary visual cortex (V1) and visually guided behavioral response is confirmed with the gap saccade paradigm. Results indicated that the variability in neural latency of V1 spike activity correlates with the gap effect on saccade latency and that the trial-to-trial variability in the state of V1 before the onset of saccade target correlates with the variability in neural and behavioral latencies.
Asunto(s)
Potenciales Evocados Visuales , Neuronas/fisiología , Movimientos Sacádicos , Corteza Visual/fisiología , Animales , Macaca mulatta , Masculino , Tiempo de Reacción , Corteza Visual/citologíaRESUMEN
A focal visual stimulus outside the classical receptive field (RF) of a V1 neuron does not evoke a spike response by itself, and yet evokes robust changes in the local field potential (LFP). This subthreshold LFP provides a unique opportunity to investigate how changes induced by surround stimulation leads to modulation of spike activity. In the current study, two identical Gabor stimuli were sequentially presented with a variable stimulus onset asynchrony (SOA) ranging from 0 to 100 ms: the first (S1) outside the RF and the second (S2) over the RF of primary visual cortex neurons, while trained monkeys performed a fixation task. This focal and asynchronous stimulation of the RF surround enabled us to analyze the modulation of S2-evoked spike activity and covariation between spike and LFP modulation across SOA. In this condition, the modulation of S2-evoked spike response was dominantly facilitative and was correlated with the change in LFP amplitude, which was pronounced for the cells recorded in the upper cortical layers. The time course of covariation between the SOA-dependent spike modulation and LFP amplitude suggested that the subthreshold LFP evoked by the S1 can predict the magnitude of upcoming spike modulation.
Asunto(s)
Potenciales de Acción/fisiología , Estimulación Luminosa , Corteza Visual/fisiología , Animales , Macaca mulatta , MasculinoRESUMEN
Visually guided saccadic eye movements are thought to involve multiple stages of processing in diverse brain structures including the primary visual cortex (V1). The variability of neural activity in each of these structures may present ambiguities for downstream stages in identifying sensory and motor signals among spontaneous discharges. The response time of saccadic eye movements made toward a visual target is correlated with the time of the first spikes in V1 that are evoked by the target (Lee et al., 2010). This suggests that downstream neurons receiving the output of V1 are faced with a challenging task of discriminating first spikes of visual response against spontaneous discharge. Here we report a novel response property of the macaque V1 neurons. Immediately before neurons discharge a burst of activity to a visual saccade target, spontaneous discharges were transiently suppressed. This suppression was maximal â¼18 ms after target onset. Based on simulations of artificial spike trains, we propose that the transient suppression enhances temporal contrast for identifying the onset of visual response by increasing the reliability of detection of response onset by downstream neurons, thereby facilitating visually guided behavioral responses.
Asunto(s)
Inhibición Neural/fisiología , Neuronas/fisiología , Tiempo de Reacción/fisiología , Movimientos Sacádicos/fisiología , Detección de Señal Psicológica/fisiología , Corteza Visual/citología , Potenciales de Acción/fisiología , Animales , Macaca mulatta , Masculino , Vías Visuales/fisiologíaRESUMEN
The spike activity of single neurons of the primary visual cortex (V1) becomes more selective and reliable in response to wide-field natural scenes compared to smaller stimuli confined to the classical receptive field (RF). However, it is largely unknown what aspects of natural scenes increase the selectivity of V1 neurons. One hypothesis is that modulation by surround interaction is highly sensitive to small changes in spatiotemporal aspects of RF surround. Such a fine-tuned modulation would enable single neurons to hold information about spatiotemporal sequences of oriented stimuli, which extends the role of V1 neurons as a simple spatiotemporal filter confined to the RF. In the current study, we examined the hypothesis in the V1 of awake behaving monkeys, by testing whether the spike response of single V1 neurons is modulated by temporal interval of spatiotemporal stimulus sequence encompassing inside and outside the RF. We used two identical Gabor stimuli that were sequentially presented with a variable stimulus onset asynchrony (SOA): the preceding one (S1) outside the RF and the following one (S2) in the RF. This stimulus configuration enabled us to examine the spatiotemporal selectivity of response modulation from a focal surround region. Although S1 alone did not evoke spike responses, visual response to S2 was modulated for SOA in the range of tens of milliseconds. These results suggest that V1 neurons participate in processing spatiotemporal sequences of oriented stimuli extending outside the RF.