RESUMEN
Apposition of the neural retina and pigment epithelium is critical to photoreceptor development and function. Interphotoreceptor retinoid-binding protein (IRBP) is a major component of the extracellular matrix separating these epithelia in the African clawed frog Xenopus laevis (Gonzalez-Fernandez et al., [1993], J. Cell Sci. 105:7-21). In the adult retina, IRBP appears to mediate the transport of hydrophobic molecules, particularly retinoids and fatty acids, within the hydrophilic extracellular domain. In this paper, we compare the distribution of IRBP and its mRNA in adult and embryonic Xenopus retina. Xenopus IRBP antisense RNA, labeled with tritium or digoxigenin, was used for in situ hybridizaton studies. For immunohistochemistry, we used an antiserum against Xenopus IRBP expressed in Escherichia coli. In the adult, we found that IRBP is synthesized at similar levels by both rods and cones. The protein is restricted to the interphotoreceptor matrix, with lesser amounts in the pigment epithelial cytoplasm. In the embryo, expression of the mRNA for IRBP is restricted to the central retina, where photoreceptor differentiation has taken place. By contrast, the protein is distributed throughout the embryonic subretinal space. Therefore, the presence of IRBP precedes photoreceptor differentiation. In summary, IRBP is synthesized by both rods and cones and may be internalized by the pigment epithelium. In the embryo, IRBP is synthesized by the central retina and diffuses through the matrix, reaching the undifferentiated peripheral retina. In view of its ligand-binding properties, diffusion of IRBP may provide the peripheral neural retina with a vehicle to transport retinoids and docosahexaenoic acid (molecules critical to normal retinal development) from the pigment epithelium.
Asunto(s)
Proteínas del Ojo/biosíntesis , ARN Mensajero/biosíntesis , Retina/metabolismo , Proteínas de Unión al Retinol/biosíntesis , Xenopus laevis/metabolismo , Animales , Retina/embriología , Retina/crecimiento & desarrollo , Xenopus laevis/embriología , Xenopus laevis/crecimiento & desarrolloAsunto(s)
Complicaciones Posoperatorias/cirugía , Prótesis e Implantes , Desprendimiento de Retina/cirugía , Curvatura de la Esclerótica , Fascia/trasplante , Femenino , Migración de Cuerpo Extraño/etiología , Migración de Cuerpo Extraño/cirugía , Humanos , Persona de Mediana Edad , Reoperación , Desprendimiento de Retina/etiología , Cuero CabelludoRESUMEN
Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular glycolipoprotein which in higher vertebrates has a 4-repeat structure and carries endogenous vitamin A and fatty acids. The location of IRBP's 1-2 binding sites for retinol is unknown. To begin to understand which repeat(s) are responsible for ligand-binding, we expressed the fourth repeat of Xenopus IRBP in E. coli to determine if it could by itself bind all-trans retinol. Our expression studies used a polyhistidine fusion domain to purify the recombinant protein directly from inclusion bodies. The fusion protein could be renatured without aggregation if refolded at a sufficiently dilute concentration (< 3 microM). The recombinant fourth repeat of Xenopus IRBP binds [3H]all-trans retinol and the fluorescence of this ligand increases 8-fold upon binding. The binding is saturable with a Kd = 0.4 microM. The expression of recombinant IRBP fragments as fusion proteins in prokaryotes will be useful for defining the structural requirements for ligand binding by this interesting protein.
Asunto(s)
Escherichia coli/genética , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Secuencias Repetitivas de Ácidos Nucleicos , Proteínas de Unión al Retinol/genética , Proteínas de Unión al Retinol/metabolismo , Animales , Sitios de Unión , Western Blotting , ADN , Electroforesis en Gel de Poliacrilamida , Proteínas del Ojo/aislamiento & purificación , Expresión Génica , Ligandos , Pliegue de Proteína , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Proteínas de Unión al Retinol/aislamiento & purificación , Espectrometría de Fluorescencia , XenopusRESUMEN
We have demonstrated that the neural retina of Xenopus laevis secretes into the extracellular matrix surrounding the inner and outer segments of its photoreceptors a glycoprotein containing hydrophobic domains conserved in mammalian interphotoreceptor retinoid-binding proteins (IRBPs). The soluble extract of the interphotoreceptor matrix contains a 124 kDa protein that cross-reacts with anti-bovine IRBP immunoglobulins. In vitro [3H]fucose incorporation studies combined with in vivo light and electron microscopic autoradiographic analysis, showed that the IRBP-like glycoprotein is synthesized by the neural retina and secreted into the interphotoreceptor matrix. A 1.2 kb Xenopus IRBP cDNA was isolated by screening a stage 42 (swimming tadpole) lambda Zap II library with a human IRBP cDNA under low-stringency conditions. The cDNA hybridizes with a 4.2 kb mRNA in adult Xenopus neural retina, tadpole heads as well as a less-abundant mRNA of the same size in brain. During development, IRBP and opsin mRNA expression correlates with photoreceptor differentiation. The translated amino acid sequence of the Xenopus IRBP clone has an overall 70% identity with the fourth repeat of the human protein. Sequence alignment with the four repeats of human IRBP showed three highly conserved regions, rich in hydrophobic residues. This focal conservation predicts domains important to the protein's function, which presumably is to facilitate the exchange of 11-cis retinal and all-trans retinol between the pigment epithelium and photoreceptors, and to the transport of fatty acids through the hydrophilic interphotoreceptor matrix.
Asunto(s)
Proteínas del Ojo , Células Fotorreceptoras/metabolismo , Proteínas de Unión al Retinol/química , Secuencia de Aminoácidos , Animales , Matriz Extracelular/química , Humanos , Datos de Secuencia Molecular , ARN Mensajero/análisis , Secuencias Repetitivas de Ácidos Nucleicos , Retina/metabolismo , Retina/ultraestructura , Proteínas de Unión al Retinol/genética , Proteínas de Unión al Retinol/metabolismo , Homología de Secuencia de Aminoácido , Xenopus laevis/genética , Xenopus laevis/metabolismoRESUMEN
Transforming growth factor-beta (TGF-beta) has recently been found in the aqueous humor. The present study was initiated to test whether the ciliary-body epithelium, the site of aqueous humor formation, is capable of producing TGF-beta. Human, rat and bovine ciliary epithelial cells were grown in tissue culture and their supernatants were tested for TGF-beta using a bioassay. After acid activation of the samples, TGF-beta activity was found in the supernatants of all three mammalian ciliary epithelial cells. Most of this activity could be blocked by a neutralizing antibody against TGF-beta type 2. Native supernatants did not contain detectable TGF-beta activity. Thus, the ciliary epithelium is capable of producing the inactive form of TGF-beta (mainly type 2) and may be a source of TGF-beta in the aqueous humor.
Asunto(s)
Cuerpo Ciliar/citología , Factor de Crecimiento Transformador beta/biosíntesis , Animales , Bioensayo , Bovinos , Línea Celular , Células Cultivadas , Cuerpo Ciliar/metabolismo , Células Epiteliales , Epitelio/metabolismo , Humanos , Ratas , Ratas Endogámicas LewRESUMEN
The antigen-specific activation of T-helper lymphocytes is dependent on the presentation of antigen in context with the gene products of major histocompatibility complex class II (MHC II). Aberrant expression of MHC II on the ciliary epithelium has been observed in uveitic eyes, which may enable these cells to interact specifically with lymphocytes and may play a role in ocular autoimmunity. Human MHC II consists of three subclasses, termed HLA DR, DP and DQ, which seem to be differentially regulated and may have different functions. The present study was initiated to investigate the dynamics of the differential MHC II expression on cultured human non-pigmented ciliary epithelial cells (NPE cells) in response to gamma-interferon by means of immunohistochemistry. NPE were isolated by a technique of selective adhesion to tissue culture substrate, and growth was induced after transfection with an origin-defective mutant of SV-40 virus DNA. NPE grown in control tissue culture medium did not express MHC class II. HLA DR and DP could be induced by incubation with low concentrations of gamma-IFN for short periods. HLA DQ was expressed only weakly even with higher doses of gamma-IFN and longer incubation periods. The differential expression of HLA DR and DP, compared to HLA DQ in response to gamma-IFN in the ciliary epithelium, is similar to observations in other non-lymphoid ocular cells, but appears to be different from the regulation of MHC II on lymphoid cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Cuerpo Ciliar/inmunología , Regulación de la Expresión Génica/fisiología , Antígenos HLA-DP/genética , Antígenos HLA-DQ/genética , Antígenos HLA-DR/genética , Línea Celular Transformada , Epitelio/inmunología , Humanos , Inmunidad Celular/genética , Inmunidad Celular/inmunología , Linfocitos T/inmunología , Uveítis/inmunologíaRESUMEN
The antigen-specific activation of T-helper lymphocytes is dependent on the presentation of antigen in context with the gene products of the major histocompatibility complex class II (MHC II). Aberrant expression of MHC II on the ciliary epithelium has been observed in uveitic eyes which may enable these cells to specifically interact with lymphocytes and may play a role in ocular autoimmunity. Human MHC II consists of three subclasses termed HLA-DR, -DP and -DQ, which seem to be differentially regulated and may have different functions. The present study was initiated to investigate the dynamics of the differential MHC II expression on cultured human non-pigmented ciliary epithelial cells (NPE cells) in response to gamma-interferon (gamma-IFN) by means of immunohistochemistry. NPE cells grown in control tissue-culture medium did not express MHC class II. HLA-DR and -DP could be induced by incubation with 100 mu/ml gamma-IFN for 3 days. HLA-DQ was expressed only weakly and at higher doses of gamma-IFN (greater than or equal to 500 mu/ml) and longer incubation periods (greater than or equal to 5 days). After removal of the gamma-IFN stimulus, all three MHC II subclasses persisted for several days. The differential expression of HLA-DR and -DP as compared with HLA-DQ in response to gamma-IFN in the ciliary epithelium is similar to observations in other non-lymphoid ocular cells but appears to be different from the regulation of MHC II expression on lymphoid cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Cuerpo Ciliar/inmunología , Antígenos HLA/análisis , Interferón gamma/farmacología , Anticuerpos Monoclonales , Línea Celular , Epitelio/inmunología , Antígenos HLA-DP/análisis , Antígenos HLA-DQ/análisis , Antígenos HLA-DR/análisis , Humanos , Técnicas para Inmunoenzimas , Proteínas RecombinantesRESUMEN
Systemic injection of bacterial endotoxin (Lipopolysaccharide, LPS) in experimental animals induces anterior uveitis without major pathological changes in other organs. The present study investigates the effect of LPS on production of inflammatory mediators in cultured bovine pigmented ciliary epithelial cells (CB-cells) by means of radioimmunoassays and bioassays. LPS was found to stimulate CB-cells to secrete prostaglandin E2 and prostacyclin (assayed as its stable metabolite 6-keto-prostaglandin F1a), but not leukotriene B4 or thromboxane A2 (assayed as its stable metabolite thromboxane B2). CB-cells produced membrane-associated interleukin 1-activity in response to LPS, but no tumor necrosis factor-activity was found after challenge of CB-cells with LPS. The direct effect of LPS on production of inflammatory mediators by cells from the anterior uvea could play a role in the pathophysiology of endotoxin-induced uveitis.