Homozygous PPT1 Splice Donor Mutation in a Cane Corso Dog With Neuronal Ceroid Lipofuscinosis.

A 10-month-old spayed female Cane Corso dog was evaluated after a 2-month history of progressive blindness, ataxia, and lethargy. Neurologic examination abnormalities indicated a multifocal lesion with primarily cerebral and cerebellar signs. Clinical worsening resulted in humane euthanasia. On necropsy, there was marked astrogliosis throughout white matter tracts of the cerebrum, most prominently in the corpus callosum. In the cerebral cortex and midbrain, most neurons contained large amounts of autofluorescent storage material in the perinuclear area of the cells. Cerebellar storage material was present in the Purkinje cells, granular cell layer, and perinuclear regions of neurons in the deep nuclei. Neuronal ceroid lipofuscinosis (NCL) was diagnosed. Whole genome sequencing identified a PPT1c.124 + 1G>A splice donor mutation. This nonreference assembly allele was homozygous in the affected dog, has not previously been reported in dbSNP, and was absent from the whole genome sequences of 45 control dogs and 31 unaffected Cane Corsos. Our findings indicate a novel mutation causing the CLN1 form of NCL in a previously unreported dog breed. A canine model for CLN1 disease could provide an opportunity for therapeutic advancement, benefiting both humans and dogs with this disorder.

Amygdala cell loss and atrophy in Alzheimer's disease.

The amygdala and its subnuclei undergo severe volumetric atrophy in Alzheimer's disease (AD). To determine whether this atrophy is due to loss of neuropil, specific neuronal populations, or both, we evaluated the number, size, and packing density of neurons and glia in the cortical and magnocellular basal amygdaloid subregions. The neuropil fraction did not change with AD in either region. Despite a mean 35% increase in cell packing density in the AD amygdala, total numbers of neurons and glia within tissue sections were reduced significantly; medium and large neurons were preferentially affected. The total number of small neurons was stable in the AD sample despite sharp reductions in nuclear size, suggesting that AD also results in pronounced amygdaloid neuronal shrinkage. Differences in the degree of cell loss between the two nuclei as well as changes in glial cell numbers are discussed in relation to characteristic AD neuropathology and relevant anatomical connectivity.

Morphometric alteration of rat myelinated fibers with aging.

The number, size, density and pathologic alterations of myelinated fibers (MF) of ventral and dorsal roots and of peroneal and sural nerves in groups of female Fischer 344 rats at 10, 20, and 30 months of age were evaluated to characterize nerve changes with old age. Except for minimal changes in the peroneal nerve, no statistically significant alteration in number of MF/nerve or in the fascicular area was associated with aging. The unaltered number was misleading since striking changes in MF size distribution, pathologic alterations of fibers and the presence of regeneration clusters suggested age-related degenerative and regenerative events. These changes were most dramatic in the ventral root, where myelin infolding, myelin separation from axon and ballooning, macrophagia and hyperplasia of Schwann cell nuclei were pronounced. Concomitant with these alterations, axonal atrophy (a reduction in caliber of axons relative to myelin spiral length or number of myelin lamellae) was demonstrated in MF of the ventral root in old age.

Structure and organization of two divergent α-amylase genes from barley.

We have isolated several α-amylase genomic clones from an Eco RI library of barley DNA in λ-Charon 32. Five of these clones exhibit unique restriction maps and differences in their abilities to hybridize with two previously characterized α-amylase cDNA probes representing two different loci, α-Amy 1 (high pI) and α-Amy 2 (low pI) on barley chromosomes 6 and 1, respectively. Stringent hybridizations indicate that four of the five genomic clones contain α-Amy 1 sequences and one contains α-Amy 2 sequences. The regions containing α-amylase genes from one representative genomic clone of each group have been sub-cloned, mapped and sequenced. S1-nuclease protection experiments indicate that the two α-amylase genes contained in these clones are functional in aleurone tissue. Transcription start sites in these genes were determined by primer extension using specific synthetic oligonucleotide primers.The DNA sequences of the two α-amylase genes, including promoter regions, are divergent, as are the predicted amino acid sequences of the mature proteins and the N-terminal "leader" peptides. The α-Amy 1 gene contains two introns while the α-Amy 2 gene has three introns. In the coding region, each gene shows 7-10% sequence divergence with respect to the previously characterized cDNA clones of the same gene type. Therefore, differences in nucleotide sequences can account for some of the isozyme variations seen between the sub-families of α-amylases and among members of the same subfamily. Although the nucleotide sequences of the promoter regions of α-Amy 1 and α-Amy 2 genes show little homology, both contain pairs of inverted repeat elements which could constitute regulatory sites.

Effect of age on vitamin E concentrations in various regions of the brain and a few selected peripheral tissues of the rat, and on the uptake of radioactive vitamin E by various regions of rat brain.

The concentrations of tocopherols in selected areas of the brains and a few peripheral tissues of 3-, 14-, and 30-month-old male Fischer 344 rats were determined by a high-performance liquid chromatographic method. Throughout the time period studied, alpha-tocopherol was the only tocopherol detected in the brain. Concentrations of alpha-tocopherol increased significantly with age in medulla and spinal cord whereas no such change was seen in other brain areas. Among the peripheral tissues, total tocopherol concentrations increased with age in the liver and adipose tissue while no significant changes were observed in the heart. The pattern of uptake of radioactive alpha-tocopherol from the serum by the various areas of the brain was similar for the 3- and 14-month-old animals even though the brains from the 14-month-old animals took up less of the radioactive compound. Measurable amounts of tocopherol esters were not present in the tissues of the 30-month-old animals.

Concentrations of vitamin E in various neuroanatomical regions and subcellular fractions, and the uptake of vitamin E by specific areas, of rat brain.

Vitamin E concentrations were determined by high-performance liquid chromatography in different anatomical regions of the brain from 3-month-old Fischer 344 rats. Gray matter from cerebellum and cervical spinal cord contained the lowest concentrations, while gray matter from the frontal cortex and thalamus had the highest concentrations of vitamin E. Radioactive alpha-tocopherol injected intravenously into the rat was readily taken up by brain although the level of uptake was very low compared with the liver. The ratios of brain-to-serum radioactivities ranged from 0.011 to 0.016 depending upon the brain region. Cerebellar gray matter is characterized by a low concentration of unlabeled alpha-tocopherol and a high level of uptake of radioactive alpha-tocopherol and thus is particularly active in the metabolism of vitamin E. Concentrations of unlabeled alpha-tocopherol were highest in microsomal and mitochondrial fractions and were the lowest in cytosol and nuclear fractions.

Brain aging in normotensive and hypertensive strains of rats. II. Ultrastructural changes in neurons and glia.

A variety of age-related changes occur in the structure of neurons in the cerebral cortex of Wistar-Kyoto and spontaneously hypertensive rats. The most marked alteration associated with increasing age was the deposition of lipofuscin pigment, primarily at the bases of apical dendrites of pyramidal neurons. While no strain-related differences in the amount of lipofuscin pigment were observed in the youngest (3 months) and in the aged (22-27 months) groups of rats, it appeared that hypertensive rats had larger pigment deposits at 12 months of age. At the ultrastructural level, neurons of the aged brains exhibited numerous nuclear invaginations and filamentous nuclear inclusions, increased amounts of Golgi complex and two types of cytoplasmic inclusions. The number of degenerative structures in the neuropil (membranous whorls, dystrophic axons and alterations in myelin sheaths) was also apparently increased in the aged brains. Neurofibrillary tangles were observed in dendritic processes of a 27-month-old Wistar-Kyoto rat. Glial cells accumulated distinctive pigment granules by which the three types of glia could be identified.

Effects of aging on the structural and permeability characteristics of cerebrovasculature in normotensive and hypertensive strains of rats.

This study demonstrates that markedly different patterns of age-related changes in blood pressure and body weight occur among normotensive Wistar-Kyoto (WKY) and Sprague-Dawley (SD) rats and spontaneously hypertensive rats (SHR). In addition, a variety of age-related structural alterations occurred in the walls of arterioles, capillaries, and venules of the frontal cortex. These changes include: (1) an increase in the thickness of the vascular wall by deposits of collagen and basal lamina which, in some cases, extended into the surrounding neuropil; (2) the presence of a flocculent material in the adventitia of intracerebral arterioles; (3) vesicular inclusions in perivascular macrophages, pericytes and smooth muscle cells which were labelled with i.v. administered horseradish peroxidase (HRP); (4) fragmentation of smooth muscle cells; and (5) accumulation of lipofuscin-like pigments in perivascular glial processes. The hypertensive rats exhibited these changes, but they were more advanced and more widely distributed throughout the cerebral cortex. The aged hypertensive rats occasionally had large bundles of 10 nm diameter, intermediate filaments in the endothelial cells. Whereas no change in blood-brain barrier permeability to HRP was observed in the aged normotensive rats, all age groups of the hypertensive rats exhibited increased permeability to HRP in the initial segment of penetrating arterioles in laminae I and II of the cerebral cortex.

Brain aging in normotensive and hypertensive strains of rats. III. A quantitative study of cerebrovasculature.

Quantitative histological techniques were used to evaluate age-related alterations in the vascularity and thickness of the cerebral cortex in normotensive and hypertensive strains of rats. In both strains, aging was accompanied by a statistically significant increase in mean minimal luminal diameter (MMLD) of capillaries in all cortical laminae. While the overall depth of the cerebral cortex was significantly decreased in aged rats of both strains, the total number of capillaries in a strip of cortex extending from the pia mater to the underlying white matter did not change between 3 months and 23-24 months. The greatest vascularity was seen in the field occupying 41-60% of the depth below the pial surface. Few differences in the laminar variations in capillary density were observed. The number and MMLD of venules were increased in aged WKY, but the aged hypertensive rats exhibited a decrease in number and MMLD of venules. No significant changes in either the number or MMLD of arterioles was observed in aged and/or hypertensive rats. The total brain weight of the hypertensive rats was significantly less than that of normotensive rats at 3 and 12 months of age but no difference was observed between the aged groups. Hypertensive rats demonstrated a significantly decreased relative brain weight (brain weight per 100 g body weight) in all age groups.