Asunto(s)
Animales , Antiinfecciosos/farmacología , Antiinfecciosos/uso terapéutico , Antibióticos Antituberculosos/farmacología , Antibióticos Antituberculosos/uso terapéutico , Ratones , ADN Bacteriano , ADN Bacteriano/genética , Datos de Secuencia Molecular , Genes Bacterianos , Genes Bacterianos/genética , Leprostáticos/farmacología , Leprostáticos/uso terapéutico , Lepra/microbiología , Lepra/tratamiento farmacológico , Mycobacterium leprae , Mycobacterium leprae/genética , Ofloxacino/farmacología , Ofloxacino/uso terapéutico , Cartilla de ADN , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Farmacorresistencia Microbiana , Resistencia a Múltiples Medicamentos , Rifampin/farmacología , Rifampin/uso terapéutico , Secuencia de BasesRESUMEN
The genetic diversity and related global distribution of 51 Mycobacterium leprae isolates were studied. Isolates were obtained from leprosy patients from 12 geographically distinct regions of the world and two were obtained from nonhuman sources. Polymerase chain reaction (PCR) followed by DNA sequencing was performed targeting the rpoT gene of M. leprae. Isolates were classified into two groups based on the number of tandem repeats composed of 6 base pairs in the rpoT gene. Isolates from Japan (except Okinawa) and Korea belonged to one group, while those from Southeast Asian countries, Brazil, Haiti and Okinawa in Japan belonged to a second genotype. M. leprae obtained from two nonhuman sources (an armadillo and a mangabey monkey) revealed the latter genotype. These results demonstrate the genetic diversity of M. leprae and the related genotype-specific distribution in the world.