RESUMEN
Mammalian cloning has been accomplished in several mammalian species by nuclear transfer. However, the production rate of cloned animals is quite low, and many cloned offspring die or show abnormal symptoms. A possible cause of the low success rate of cloning and abnormal symptoms in many cloned animals is the incomplete reestablishment of DNA methylation after nuclear transfer. We first analyzed tissue-specific methylation patterns in the placenta, skin, and kidney of normal B6D2F1 mice. There were seven spots/CpG islands (0.5% of the total CpG islands detected) methylated differently in the three different tissues examined. In the placenta and skin of two cloned fetuses, a total of four CpG islands were aberrantly methylated or unmethylated. Interestingly, three of these four loci corresponded to the tissue-specific loci in the normal control fetuses. The extent of aberrant methylation of genomic DNA varied between the cloned animals. In cloned animals, aberrant methylation occurred mainly at tissue-specific methylated loci. Individual cloned animals have different methylation aberrations. In other words, cloned animals are by no means perfect copies of the original animals as far as the methylation status of genomic DNA is concerned.
Asunto(s)
Clonación de Organismos , Metilación de ADN , Animales , Animales Modificados Genéticamente , Núcleo Celular/genética , Islas de CpG , ADN/metabolismo , Proteínas de Unión al ADN/metabolismo , Riñón/metabolismo , Ratones , Técnicas de Transferencia Nuclear , Placenta/metabolismo , Placentación , Piel/metabolismoAsunto(s)
Apoptosis , Neoplasias , Transferasas Alquil y Aril/antagonistas & inhibidores , Animales , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Farnesol/análogos & derivados , Farnesol/uso terapéutico , Farnesiltransferasa , Humanos , Neoplasias/tratamiento farmacológico , Neoplasias/patología , Proteína p53 Supresora de Tumor , Proteínas rasRESUMEN
Two cases of postinfarction oozing type left ventricular rupture and a case of oozing type left ventricular rupture due to catheter perforation for left ventriculography are reported. The technique used to repair the rupture is fibrin glue-oxycellulose fixation method. The post operative course of three cases were uneventful. But left ventricular aneurysm remained in two cases.
Asunto(s)
Celulosa Oxidada/uso terapéutico , Adhesivo de Tejido de Fibrina/uso terapéutico , Rotura Cardíaca Posinfarto/terapia , Anciano , Cateterismo Cardíaco/efectos adversos , Femenino , Ventrículos Cardíacos , Técnicas Hemostáticas , Humanos , MasculinoAsunto(s)
Antineoplásicos/uso terapéutico , Citocinas/uso terapéutico , Neoplasias/terapia , Ensayos Clínicos como Asunto , Terapia Combinada , Ciclofosfamida/administración & dosificación , Femenino , Factor Estimulante de Colonias de Granulocitos/administración & dosificación , Humanos , Interleucina-2/administración & dosificación , MasculinoAsunto(s)
Citocinas/uso terapéutico , Neoplasias/terapia , Animales , Citotoxicidad Inmunológica , Fibrosarcoma/terapia , Humanos , Linfocitos/inmunología , Ratones , Neoplasias/inmunología , Receptores de Transferrina , Retroviridae/genética , Transfección/métodos , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Phase II study of a new 5-fluorouracil derivative, 5'-deoxy-5-fluorouridine (5'-DFUR), was performed with oral administration. Forty-nine patients with advanced cancer of the digestive organs, lung and breast were entered, and 8 institutions in Hokkaido were involved. 5'-DFUR was administered three or four times a day at a daily dosage of 600 to 1200 mg. Partial response was observed in three gastric cancer cases and two breast cancer cases out of 39 evaluable cases, and minor response was observed in one colorectal cancer case. Overall response rate was 12.8%, 15.8% in gastric cancer and 66.7% in breast cancer. Side effects were observed in 15 cases out of 45 (33.3%), which mainly consisted of gastro-intestinal disturbances such as diarrhea.
