Asunto(s)
Aneurisma Falso/diagnóstico , Fístula Arteriovenosa/diagnóstico , Edad Gestacional , Placenta Accreta/fisiopatología , Aborto Espontáneo , Adulto , Aneurisma Falso/etiología , Aneurisma Falso/terapia , Fístula Arteriovenosa/etiología , Embolización Terapéutica , Femenino , Humanos , Histerectomía , EmbarazoAsunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Histerectomía , Tumor Trofoblástico Localizado en la Placenta , Neoplasias Uterinas , Adulto , Biopsia , Terapia Combinada , Ciclofosfamida/administración & dosificación , Dactinomicina/administración & dosificación , Etopósido/administración & dosificación , Femenino , Humanos , Metotrexato/administración & dosificación , Embarazo , Tumor Trofoblástico Localizado en la Placenta/tratamiento farmacológico , Tumor Trofoblástico Localizado en la Placenta/secundario , Tumor Trofoblástico Localizado en la Placenta/cirugía , Neoplasias Uterinas/tratamiento farmacológico , Neoplasias Uterinas/patología , Neoplasias Uterinas/cirugía , Vincristina/administración & dosificaciónRESUMEN
We previously investigated tissue specificity and temporal changes in expression of five human endogenous retroviruses (HERVs), including syncytin/ERVWE1 and syncytin 2. In the current study, we examined the cellular localization and quantified the transcripts of five HERVs, syncytin, syncytin 2, HERV-H7/F(XA34), HERV-Fb1, and HERV-HML6-c14, in order to elucidate their physiological and etiological roles in the placenta and in pregnancy-induced hypertension (PIH), respectively. In situ hybridization revealed trophoblast-specific transcription of all five HERVs. Consistent with a previous immunohistochemical analysis, syncytin 2 transcripts were detected only in cytotrophoblasts. All the HERVs except HERV-HML6-c14 (HML6-c14) were predominantly localized in the cytoplasm of syncytiotrophoblasts and/or cytotrophoblasts. Quantitative analysis showed that transcriptional levels of these four HERVs were lower in placentas obtained from pregnant women with PIH (n=22) than in those from normotensive pregnant women (n=87) and that the differences were statistically significant (p=0.001, 0.01, <0.001, and 0.04 for syncytin, syncytin 2, HERV-H7/F(XA34), and HERV-Fb1, respectively). In contrast to the other HERVs, HML6-c14 transcripts localized to the nucleus and the average transcriptional level of HML6-c14 was higher in PIH placentas than in control placentas from normotensive pregnant women, although the differences did not reach significance (p=0.19). These results suggest that placenta-specific HERVs may have some function in the human placenta and that their reduced expression in PIH placentas is not merely a secondary effect derived from the pathology of PIH placentas.