[The Mopeia virus induces pH-dependent "fusion from within" in a BHK-21 cell culture]. / Virus Mopeiia indutsiruet pH-zavisimoe "sliianie iznutri" kul'tury kletok BHK-21.

The capacity of BHK-21 cell culture to produce Mopeia virus (Arenaviridae family) to form syncytium upon acidification of the culture medium to pH 5.5 and lower was demonstrated. The cell fusion requires their active virus production: a virus titre in the culture medium must be at least 10(5) PEU/ml. The inhibition of virus multiplication with ammonium chloride as well as treatment of the cells before the medium acidification with immune serum reduced syncytium formation markedly. No cell fusion was observed upon acidification of the medium immediately after virus adsorption to cells. Thus, the observed cell fusion under the influence of the virus is an "internal fusion" and confirms our previous data on the endocytosis mode of arenavirus penetration into the cell.

[Lysosomotropic agents inhibit the penetration of arenaviruses into a culture of BHK-21 and Vero cells]. / Lizosomotropnye agenty ingibiruiut proniknovenie arenavirusov v kul'turu kletok BHK-21 i Vero.

Lysosomotropic agents (NH4Cl, amantadine, chloroquine, monensin) which prevent acidification of intracellular vacuoles, when introduced into the culture medium before or during inoculation of cells (BHK-21, Vero) with arenaviruses inhibit reproduction of these viruses completely or significantly. Mozambique virus proved to be 10 times more sensitive to the effect of lysosomotropic agents than Pichinde and Lassa viruses. Thus, arenaviruses have a pH-dependent stage at the beginning of the reproduction cycle which is indirectly indicative of their penetration into cells by receptor endocytosis.

[Importance of a self-care system in diabetes mellitus in preventing complications and preserving the patient's work capacity]. / Znachenie sistemy samokontrolia sakharnogo diabeta v profilaktike oslozhnenii i sokhranenii trudosposobnosti bol'nykh.

A system of self-control of the patients nutrition using the V. S. Vorobyov calculator was introduced to prevent early complications in diabetes mellitus patients, 112 patients were followed up during 5 months. 35 of them were administered insulin, 65 sugar-reducing drugs per os, and 12 patients were given diets. Introduction of the self-control system in the nutrition of diabetes mellitus patients made it possible to achieve stable compensation in 89.4% patients, reduction of the dose or withdrawal of oral sugar-reducing drugs, and reduction of the insulin dose. It is suggested to devise a unified system for training diabetes mellitus patients, with a special section on training self-control methods in nutrition.