RESUMEN
BACKGROUND: Bacteriophage therapy is considered one of the most attractive alternatives to antibiotic treatment, which may be significant due to the rising number of antibiotic-resistant bacterial strains. Patients with cancer frequently suffer bacterial infections resulting from immunosuppression caused by anticancer treatment; thus they constitute a considerable group of patients subjected to phage therapy. In this study, we investigated the influence of bacteriophages on the migration of human leukemia (HL-60) cells. Results of these studies provide data regarding phage treatment of patients with cancer, especially with this type of leukemia. MATERIALS AND METHODS: The influence of phage preparation on migration of HL-60 leukemia cells was evaluated with BD Bioscience Migration Chambers. RESULTS: Bacteriophages have no influence on migration of HL-60 cells. The only phage preparation which stimulated migration of HL-60 cells was Staph.liz, specific to S. aureus, however, the molecular basis of these interactions cannot be currently explained. CONCLUSION: Results of our studies may be in line with previous data indicating that phage therapy is safe for patients with cancer.
Asunto(s)
Bacteriófagos/fisiología , Movimiento Celular , Infecciones por Escherichia coli/terapia , Infecciones por Pseudomonas/terapia , Infecciones Estafilocócicas/terapia , Escherichia coli/fisiología , Infecciones por Escherichia coli/microbiología , Células HL-60 , Humanos , Técnicas In Vitro , Lipopolisacáridos/metabolismo , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/fisiología , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/fisiologíaRESUMEN
Bacteriophages are viruses that infect bacteria. It was shown that bacteriophage therapy is an effective method of combating bacterial infections, including infections caused by antibiotic-resistant bacterial strains. One of the main obstacles to widespread use of phage preparations is limited knowledge regarding the influence of bacteriophages on human organisms. In our study, we evaluated whether application of phage preparations impair bactericidal activities of human phagocytes (granulocytes and monocytes). In our study, we used preparations of phages T2 and T4 specific to Escherichia coli and A3 phage specific to Staphylococcus aureus. We found that bacteriophage preparations do not influence intracellular killing of bacteria by human phagocytes. The effect is irrespective of phage preparation type (lysate, purified phage preparation), phage titer of the preparation, and whether bacteria phagocytosed by phagocyte cells are sensitive or insensitive to phage (bacteriophages homologous and heterologous to bacteria). Although the results of our study are preliminary, they support previous data indicating safety of therapeutic application of phages.
