RESUMEN
BACKGROUND: Divalent metal transporter 1 (DMT1) is a transmembrane glycoprotein which mediates the proton-coupled transport of a variety of divalent metal ions. Two isoforms, which differ by the presence (DMT1-IRE) or absence (DMT1-nonIRE) of an iron-responsive element (IRE) in their 3' untranslated region, are implicated in apical iron transport and endosomal iron transport respectively. Although the expression pattern of DMT1 isoforms is tissue specific in adult, data regarding its expression in embryonic tissues are lacking. METHODS: Semiquantitative RT-PCR and immunohistochemistry were used to study the mRNA and protein expression of both DMT1 isoforms in embryonic tissues between 8 and 14 weeks gestational age. RESULTS: DMT1-IRE and DMT1-nonIRE expressions were ubiquitous in embryonic tissues examined. In the lung, statistically significant correlations were found between the levels of DMT1 isoform expression and gestational age. In the placenta, DMT1-IRE was the predominantly expressed isoform. Both isoform proteins were localized in embryonic epithelial cellular membrane. CONCLUSION: Both DMT1 isoforms are ubiquitously expressed in embryonic tissues in the first trimester. Predominant DMT1-IRE isoform expression in placenta suggests an iron-regulatory mechanism reminiscent of that in the adult duodenum. Epithelial distributions of both DMT1 isoforms are associated with the absorptive or excretory functions of the expressed tissues.
Asunto(s)
Proteínas de Transporte de Catión/biosíntesis , Proteínas de Transporte de Catión/química , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica , Proteínas de Unión a Hierro/biosíntesis , Proteínas de Unión a Hierro/química , Placenta/embriología , Adulto , Transporte Biológico , Cartilla de ADN/química , Femenino , Edad Gestacional , Humanos , Inmunohistoquímica , Mucosa Intestinal/metabolismo , Iones , Riñón/metabolismo , Pulmón/metabolismo , Placenta/metabolismo , Embarazo , Primer Trimestre del Embarazo , Isoformas de Proteínas , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de Tiempo , Distribución TisularRESUMEN
Duplications of alimentary tract are uncommon congenital anomalies that are usually identified during the pediatric age. However, a minority of cases remain unsuspected until adulthood. They may be cystic or tubular in appearance and characteristically arise from the mesenteric border of the intestine. This paper reports a 37-year-old male with a huge cystic duplication of the ascending colon. In facing such a huge abdominal cystic tumor, differential diagnoses including duplication cyst, mesenteric cyst, choledochal cyst, giant diverticulum, and cystic tumor of the pancreas should be considered. Computerized tomography is a good tool for delineating the relationship between the tumor and peripheral structures. Surgery not only provides treatment but also final pathological diagnosis. Complete resection of a colonic duplication is necessary because of potential malignant change of the lesion.
Asunto(s)
Colon/anomalías , Enfermedades del Colon/cirugía , Quistes/cirugía , Adulto , Colon/cirugía , Enfermedades del Colon/diagnóstico , Enfermedades del Colon/patología , Quistes/diagnóstico , Quistes/patología , Diagnóstico Diferencial , Humanos , MasculinoAsunto(s)
Neoplasias Duodenales/cirugía , Duodenoscopía , Hemorragia Gastrointestinal/cirugía , Pólipos Intestinales/cirugía , Lipoma/cirugía , Anciano , Neoplasias Duodenales/patología , Hemorragia Gastrointestinal/patología , Humanos , Mucosa Intestinal/patología , Mucosa Intestinal/cirugía , Pólipos Intestinales/patología , Lipoma/patología , MasculinoRESUMEN
A new cell line, ME, has been established from a melanoma of the palatal mucosa. The cultured monolayer of cells was fusiform and melanin-producing. The cells were highly tumorigenic and metastatic in nude mice. The xenographic tumors resembled the original tumor in morphology, melanin production, and the expression of S-100 and HMB-45 antigens. The metaphase karyotype of ME indicated multiple aberrations of chromosomes 2, 3, 5, 7-11, 13, 19, 21 and X. A homozygous loss of the p16/MTS1 gene during the establishment of ME correlated with karyotypic evidence of chromosome 9 abnormalities. Absence of nm23 protein expression and elevated expression of CD44 protein (indicative of metastatic phenotypes) were demonstrated in primary and xenographic tumors. ME cells could be valuable in developing novel therapeutic strategies for oral melanoma.
Asunto(s)
Melanoma/patología , Neoplasias de la Boca/patología , Nucleósido-Difosfato Quinasa , Células Tumorales Cultivadas/patología , Anciano , Animales , Antígenos de Neoplasias , Técnicas de Cultivo de Célula , Aberraciones Cromosómicas , Trastornos de los Cromosomas , Inhibidor p16 de la Quinasa Dependiente de Ciclina/análisis , Citoplasma/química , Femenino , Humanos , Receptores de Hialuranos/análisis , Inmunohistoquímica , Cariotipificación , Melaninas/análisis , Melanoma/química , Melanoma/genética , Antígenos Específicos del Melanoma , Ratones , Ratones Desnudos , Proteínas de Unión al GTP Monoméricas/análisis , Neoplasias de la Boca/química , Neoplasias de la Boca/genética , Nucleósido Difosfato Quinasas NM23 , Proteínas de Neoplasias/análisis , Trasplante de Neoplasias , Neoplasias Experimentales/patología , Reacción en Cadena de la Polimerasa , Proteínas S100/análisis , Análisis de Secuencia de ADN , Factores de Transcripción/análisis , Células Tumorales Cultivadas/químicaRESUMEN
The expression of p53 and p21WAF1 in 53 oral verrucous leukoplakias (OVLs), mostly non-dysplastic lesions, was investigated to ascertain the role of such events in malignant conversion. Immunohistochemical analysis revealed aberrant p53 and p21WAF1 immunoreactivity in 51% (27 cases) and 75% (40 cases), respectively. After an average follow-up period of three and a half years, histopathological examination revealed that 22 (42%) cases had developed oral squamous cell carcinoma (OSCC), 14 (26%) cases had undergone recurrence, and 17 (32%) cases were free of disease. The oncogenic potential of this subset of premalignant lesions warrants attention. A significant difference in the frequency of OSCC progression/recurrence was noted in lesions bearing aberrant immunoreactivity of either p53 (93% vs 42%; P=0.00008) or p21WAF1 (80% vs 32%; P=0.002) in comparison with lesions without immunoreactivity. This study suggested that the aberrant immunoreactivity of p53 and p21WAF1 may represent important alterations of OVL and could affect the outcome of this lesion.
Asunto(s)
Ciclinas/genética , Inhibidores Enzimáticos/metabolismo , Leucoplasia Bucal/patología , Proteína p53 Supresora de Tumor/genética , Adulto , Anciano , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Transformación Celular Neoplásica/genética , Transformación Celular Neoplásica/patología , Estudios de Cohortes , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Estudios de Seguimiento , Regulación Neoplásica de la Expresión Génica , Humanos , Inmunohistoquímica , Leucoplasia Bucal/genética , Persona de Mediana Edad , Neoplasias de la Boca/genética , Neoplasias de la Boca/patología , Recurrencia Local de Neoplasia/genética , Recurrencia Local de Neoplasia/patología , Lesiones Precancerosas/genética , Lesiones Precancerosas/patología , Pronóstico , TaiwánRESUMEN
Benign metastasizing leiomyoma (BML) refers to benign pulmonary neoplasm associated with a previous or coincident history of uterine leiomyomata. We report the case of a 56-year-old postmenopausal woman with a 4-year history of multiple benign leiomyomatous lesions in bilateral lungs and the uterus. The tumor cells from the lungs and uterus were focally immunoreactive for HMB-45 antibody and progesterone receptor. Immunoreactivity to HMB-45 is well known in smooth muscle cells of hamartomatous neoplasms, such as angiomyolipoma and lymphangioleiomyomatosis, but has not been reported in BML previously. These features suggest a multifocal hamartomatous histogenesis rather than metastasis in the present case.
Asunto(s)
Leiomiomatosis/diagnóstico , Neoplasias Pulmonares/diagnóstico , Neoplasias Primarias Secundarias/diagnóstico , Antígenos de Neoplasias/análisis , Femenino , Humanos , Leiomiomatosis/química , Leiomiomatosis/inmunología , Neoplasias Pulmonares/química , Neoplasias Pulmonares/inmunología , Neoplasias Pulmonares/secundario , Antígenos Específicos del Melanoma , Persona de Mediana Edad , Proteínas de Neoplasias/análisis , Neoplasias Primarias Secundarias/química , Neoplasias Primarias Secundarias/inmunología , Receptores de Progesterona/análisis , Neoplasias Uterinas/química , Neoplasias Uterinas/inmunología , Neoplasias Uterinas/patologíaRESUMEN
Color Doppler ultrasound (US) was performed in 153 patients (including 102 with lung cancer and 51 with benign lesions) to assess pulsatile flow signals in thoracic lesions. The values of resistive index (RI) and pulsatility index (PI) of color Doppler US pulsatile flow signals in lung cancers and benign lesions were measured, analyzed, and compared. In the enrolled 153 patients with thoracic lesions, 61 lung cancers and 34 benign lesions had detectable color Doppler US pulsatile flow signals, and lung cancers had lower RI and PI values than benign lesions (RI: 0.70+/-0.03 vs. 0.79+/-0.04, p < 0.05; PI: 1.61+/-0.15 vs. 2.44+/-0.25, p < 0.005). However, overlapping RI and PI values in lung cancers and benign lesions somewhat limited color Doppler US pulsatile flow signals to differentiate lung cancers from benign lesions. Further analysis of RI and PI values in subgroups of lung cancers [squamous cell carcinoma (SCC, n = 34), adenocarcinoma (AC, n = 18), and small-cell lung cancer (SCLC, n = 6)] and benign lesions [cavitary benign lesions (CBL, n = 8), and noncavitary benign lesions (NCBL, n = 26)] revealed that all different cell types of lung cancers (SCC, AC, and SCLC), indeed, had lower RI and PI values than NCBL (for RI, all p < 0.01; for PI, all p< or =0.001). Moreover, the mean RI and PI values showed a significant incremental decrease from NCBL (mean RI, PI = 0.88, 2.94) toward SCC and AC (for SCC, mean RI, PI = 0.71, 1.68; for AC, mean RI, PI = 0.68, 1.67) and, finally, to SCLC (mean RI, PI = 0.62, 1.05). In contrast, CBL had relatively lower RI and PI values than AC and SCLC (for CBL, mean RI, PI = 0.53, 0.80; both p > 0.05 for RI and PI), and even a significant difference from SCC (p < 0.05 for RI and PI). We conclude that color Doppler US pulsatile flow signal is somewhat limited to differentiate lung cancers from benign lesions, but provides a noninvasive in vivo model to assess the neovascularity intensity of lung cancers.
Asunto(s)
Adenocarcinoma/fisiopatología , Carcinoma de Células Pequeñas/fisiopatología , Carcinoma de Células Escamosas/fisiopatología , Enfermedades Pulmonares/fisiopatología , Neoplasias Pulmonares/fisiopatología , Ultrasonografía Doppler en Color , Adenocarcinoma/irrigación sanguínea , Adenocarcinoma/diagnóstico por imagen , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Biopsia con Aguja , Velocidad del Flujo Sanguíneo , Carcinoma de Células Pequeñas/irrigación sanguínea , Carcinoma de Células Pequeñas/diagnóstico por imagen , Carcinoma de Células Escamosas/irrigación sanguínea , Carcinoma de Células Escamosas/diagnóstico por imagen , Diagnóstico Diferencial , Femenino , Estudios de Seguimiento , Humanos , Enfermedades Pulmonares/diagnóstico por imagen , Neoplasias Pulmonares/irrigación sanguínea , Neoplasias Pulmonares/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Flujo Pulsátil , Estudios RetrospectivosRESUMEN
BACKGROUND: Traditional pleurodesis for malignant pleural effusion is performed by large-bore chest tube insertion with the instillation of sclerosing agents after the compressed lung re-expansion and pleural fluid drainage of 100-150 ml/day. This study was carried out to evaluate the possibility of rapid sclerotherapy for malignant pleural effusions by insertion of a small-bore Elecath tube (12-French) under ultrasound guidance and intrapleural injection of bleomycin 60 IU. METHODS: Twenty-six patients, with 28 cytopathologically proven malignant pleural effusions (two patients had bilateral pleural effusions) and receiving the insertion of the Elecath tube for drainage, were included in our series. This rapid and short-term sclerosing method was performed and completed by intrapleural injection of bleomycin when the pleural effusion had been clearly drained by the small-bore Elecath tube and the compressed lung had fully re-expanded on follow-up chest radiographs. RESULTS: Twenty patients with 22 pleural effusions underwent the intrapleural injection of bleomycin, with the results of pleurodesis being complete response 41% (9/22), partial response 36% (8/22) and failure 23% (5/22). Interestingly, among the 17 successful procedures of pleurodesis (complete response and partial response), 71% (12) procedures could be completed within 2 days (seven within one day and five within 2 days). The remaining unsuccessful procedures carried out on six patients without the injection of bleomycin were due to a non-re-expanded lung (n = 3) and inadequate drainage (n = 3); of these, four patients also received the large-bore chest tube insertion after the removal of the Elecath tube, but the compressed lung still could not re-expand. The complications of the bleomycin injection were fever [77% (17/22)], vomiting [14% (3/22)] and hiccup [5% (1/22)]. CONCLUSION: The method of rapid sclerotherapy for malignant pleural effusions by small-bore Elecath tube is promising, with a success rate achieving 77%, usually within 2 days.
Asunto(s)
Bleomicina/administración & dosificación , Drenaje/instrumentación , Derrame Pleural Maligno/terapia , Escleroterapia/instrumentación , Femenino , Humanos , Intubación , Neoplasias Pulmonares/complicaciones , Masculino , Derrame Pleural Maligno/diagnóstico por imagen , Soluciones Esclerosantes/administración & dosificación , UltrasonografíaRESUMEN
A tumor in the breast of a 67-year-old male was resected. It was grossly circumscribed and unencapsulated. Histologically, it was composed of plump and long bipolar, spindle cells arranged in fascicular clusters with intervening broad collagen bands. The cells lacked pleomorphism, and no mitotic figures were found. The margin was that of pushing border type. No epithelial component was seen in the tumor. Immunohistochemically, the tumor cells expressed variable reactivity to vimentin, desmin and smooth muscle actin and were negative for S-100 protein and keratin. Electron microscopy showed cell differentiation between fibroblast and smooth muscle cells.
Asunto(s)
Neoplasias de la Mama/patología , Neoplasias de Tejido Muscular/patología , Anciano , Femenino , Humanos , Inmunohistoquímica , MasculinoRESUMEN
Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.
Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/genética , Codón , Genes ras , Neoplasias Pulmonares/genética , Mutación Puntual , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la PolimerasaRESUMEN
BACKGROUND: The mechanically incompetent lower esophageal sphincter (LES) plays a key role in patients with gastroesophageal reflux disease. The purpose of the study was to assess the pressure changes of LES in rabbits by intraabdominal injection of Teflon paste (Polytetrafluoroethylene) at the gastroesophageal junction. METHODS: New Zealand white breed of rabbits were used in these studies. The anesthetized rabbit was injected with a 21-gauge needle and a syringe loaded with Teflon paste. The injection sites were around the gastroesophageal junction. LES pressure was measured by conventional methods using a water-filled infused system. The pressure gradient was measured immediately before and after the injection as well as by a weekly measurement over four weeks. The histologic characteristics of the injection sites were studied four weeks later. RESULTS: The mean pressure gradient of LES of twelve rabbits of immediate preinjection and post-injection was 29.71 +/- 8.10 mmHg and 37.58 +/- 10.69 mmHg (mean +/- S.D.), respectively (p value, 0.0329) Animals were followed upifor from one to four weeks, and were then sacrificed. The mean pressure gradient of LES of the twelve rabbits in the first week, the second week, the third week and the fourth week was 37.80 +/- 11.36 mmHG, 35.77 +/- 3.54 mmHg, 33.42 +/- 4.95 mmHg and 32.68 +/- 4.62 mmHg (mean +/- S.D.), respectively. Compared to pre-injection data, a significant difference was found in the first week and the second week (p value, 0.0342 and 0.0281, respectively). Gross examination of the gastroesophageal regions showed a welldefined Teflon mass of firm consistency at the site of the injection. Histological examination showed encapsulation of the implant by a thin layer of fibrous tissue and a benign foreign body granulomatous reaction with round cells surrounding the implant. CONCLUSIONS: It is technically feasible to produce experimentally pressure changes of LES in rabbits by intraabdominal injection of Teflon paste. Nevertheless, the clinical validity on patients with gastroesophageal reflux induced by the incompetence of LES remains to be verified.
Asunto(s)
Unión Esofagogástrica/fisiología , Politetrafluoroetileno/farmacología , Animales , Unión Esofagogástrica/efectos de los fármacos , Presión , ConejosRESUMEN
One hundred eighty-eight patients with 191 lung cancers were collected retrospectively to evaluate the diagnostic results and to determine the accuracy of cytologic diagnoses obtained from ultrasound-guided fine-needle aspiration biopsy (US-guided FNAB), and to discuss the necessity of large-bore tissue core needle biopsy. All 188 patients underwent US-guided FNAB, and 20 patients with 21 lung tumors also underwent US-guided tissue-core needle biopsy. Using US-guided FNAB alone, the positive cytologic results and correct cytologic diagnoses were 91% (174 of 191) and 71% (37 of 52). If both US-guided FNAB and selected US-guided tissue core needle biopsy (n = 21) were evaluated, the positive cytologic or histologic results and correct cytologic or histologic diagnoses were 94% (180 of 191) and 80% (45 of 57), respectively. Analyzing the disagreement between the cytologic results and histologic diagnoses (n = 15), we found that the disagreement usually occurred in the specimens with poorly differentiated carcinomas (nonspecific cell type) (53% [8 of 15]); of these, two patients (13% [2 of 15], small cell carcinoma) would have a change in treatment. The complications of US-guided FNAB were pneumothorax (n = 3), hemoptysis (n = 1), hemothorax (n = 1), and suspected tract metastasis (n = 1). We conclude that US-guided FNAB has a high diagnostic yield in lung cancers, and US-guided tissue core needle biopsy is only necessary in patients whose cytologic results are negative or who have poorly differentiated carcinomas.
Asunto(s)
Biopsia con Aguja/métodos , Neoplasias Pulmonares/diagnóstico por imagen , Neoplasias Pulmonares/patología , Adulto , Anciano , Anciano de 80 o más Años , Biopsia con Aguja/efectos adversos , Hemoptisis/etiología , Hemotórax/etiología , Humanos , Pulmón/diagnóstico por imagen , Persona de Mediana Edad , Neumotórax/etiología , Estudios Retrospectivos , UltrasonografíaRESUMEN
Sarcoma of the hypopharynx has been reported very rarely in the literature, only six cases having been found among all head and neck malignancies reported to SEER (Surveillance, Epidemiology, and End Results) during 1973-1987. We report a 14-year-old boy with a huge malignant soft tissue sarcoma arising from the hypopharynx. Tracheostomy and feeding gastrostomy were performed as emergency life-saving procedures. Surgical resection had been attempted, but abandoned. Because of the rapidity of tumor growth, we gave the patient a course of accelerated radiotherapy (170 cGy/fraction, two fractions per day) with a total dose of 7140 cGy within one month. A series of endoscopy and imaging studies demonstrated complete regression of the tumor, and the patient is currently alive without evidence of disease 3.5 years after treatment. We conclude that for an unresectable tumor without distant metastasis, radiation therapy may be tried. The time, dose, and fractionation of radiotherapy should be carefully designed and individualized.
Asunto(s)
Neoplasias Hipofaríngeas/radioterapia , Sarcoma/radioterapia , Adolescente , Humanos , Neoplasias Hipofaríngeas/diagnóstico , Neoplasias Hipofaríngeas/patología , Imagen por Resonancia Magnética , Masculino , Dosificación Radioterapéutica , Sarcoma/diagnóstico , Sarcoma/patologíaRESUMEN
Sixty-eight patients with thoracic lesions (48 with lung cancer and 20 with benign lesions) underwent color Doppler ultrasound (US) examinations. Of those, 21 patients (13 with lung cancer and eight with benign lesions) also received resections, and the correlation between color Doppler US signals and resected histologic specimens was evaluated. Our results showed that three patterns of color Doppler US signals could be detected and confirmed: pulsatile flow (artery), constant flow, and triphasic flow (pulmonary vein). Among the 48 patients with lung cancer, pulsatile flow, constant flow, and/or triphasic flow were detected in 34 (71%), 24 (50%), and 14 (29%), respectively. Among the 20 patients with benign lesions, only pulsatile flow and/or triphasic flow were detected in nine (45%) and eight (40%), respectively. From the correlation between color Doppler US signals and histologic specimens, constant flow was representative of the true neovascularity of lung cancers, and it was valuable for differentiating lung cancers from benign lesions (p = 0.00008, sensitivity = 0.50, and specificity = 1.0). Although color Doppler US still had some limitations in detecting blood vessels in thoracic lesions, the correlation between the vascularity represented by color Doppler US signals and histologic specimens was excellent. We conclude that color Doppler US is a valuable method for assessing blood flow in thoracic lesions and differentiating lung cancers from intrapulmonary benign lesions.
Asunto(s)
Enfermedades Pulmonares/diagnóstico por imagen , Enfermedades Pulmonares/patología , Ultrasonografía Doppler en Color , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Enfermedades Pulmonares/cirugía , Neoplasias Pulmonares/diagnóstico por imagen , Neoplasias Pulmonares/patología , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Flujo PulsátilRESUMEN
Ultrasound-guided fine-needle aspiration biopsy (US-guided FNAB) was performed in 40 patients with solitary pulmonary nodules (SPNs) for evaluation of diagnostic results and complication rates. The final diagnoses of the 40 patients included 30 malignancies and 10 benign lesions. Using US-guided FNAB, the diagnostic yields were 97% (29/30) in malignancies and 60% (6/10) in benign lesions. Of the 29 patients with cytologically proven malignancies, 12 underwent surgical resection. The correlation between cytological results and histologic diagnoses in these 12 was excellent (100%). The size of the nodule did not affect the diagnostic rate or complication rate. Only two patients (5%) developed minimal pneumothorax after US-guided FNAB. We conclude that US-guided FNAB is a useful, safe, and convenient diagnostic tool for SPN, and that malignant pulmonary nodules are more easily diagnosed than benign nodules.
Asunto(s)
Biopsia con Aguja/métodos , Neoplasias Pulmonares/patología , Nódulo Pulmonar Solitario/patología , Ultrasonografía Intervencional , Adulto , Anciano , Anciano de 80 o más Años , Técnicas Bacteriológicas , Biopsia con Aguja/efectos adversos , Carcinoma/diagnóstico por imagen , Carcinoma/patología , Carcinoma/cirugía , Carcinoma de Células Escamosas/diagnóstico por imagen , Carcinoma de Células Escamosas/patología , Carcinoma de Células Escamosas/cirugía , Técnicas de Diagnóstico Quirúrgico , Femenino , Estudios de Seguimiento , Humanos , Neoplasias Pulmonares/diagnóstico por imagen , Neoplasias Pulmonares/cirugía , Masculino , Persona de Mediana Edad , Neumotórax/etiología , Sensibilidad y Especificidad , Nódulo Pulmonar Solitario/diagnóstico por imagen , Nódulo Pulmonar Solitario/cirugía , Esputo/citología , Esputo/microbiología , Tuberculosis Pulmonar/diagnóstico por imagen , Tuberculosis Pulmonar/patología , Ultrasonografía Intervencional/instrumentación , Ultrasonografía Intervencional/métodosRESUMEN
Thirty-three patients with anterior mediastinal masses underwent percutaneous ultrasonically guided needle biopsy (UGNB), including ultrasonically guided aspiration biopsy (UGAB) in all 33 patients and ultrasonically guided cutting biopsy (UGCB) in 13 patients. Using UGAB alone, the diagnostic rate of anterior mediastinal masses was 52% (17/33); if both the UGAB and UGCB methods were used, the diagnostic rate could achieve 79% (26/33). If the anterior mediastinal masses were divided into carcinomatous (n = 15) and non-carcinomatous (n = 18) groups, we found that the carcinomatous group was more easily diagnosed by UGAB than the non-carcinomatous group (87% vs 22%, p < 0.01) and UGCB was more valuable and helpful than UGAB in the diagnosis of non-carcinomatous mediastinal masses (75% vs 22%). One episode of injury to the aortic wall occurred after a UGCB. Our results show that carcinomatous mediastinal masses can be easily diagnosed by UGAB, and UGCB is often necessary in the diagnosis of non-carcinomatous mediastinal masses.
Asunto(s)
Biopsia con Aguja , Enfermedades del Mediastino/patología , Neoplasias del Mediastino/patología , Adolescente , Adulto , Anciano , Biopsia con Aguja/instrumentación , Biopsia con Aguja/métodos , Diagnóstico Diferencial , Femenino , Humanos , Masculino , Enfermedades del Mediastino/diagnóstico , Enfermedades del Mediastino/diagnóstico por imagen , Neoplasias del Mediastino/diagnóstico , Neoplasias del Mediastino/diagnóstico por imagen , Persona de Mediana Edad , Sensibilidad y Especificidad , UltrasonografíaRESUMEN
Disagreement over the neuroanatomical substrate of associative visual agnosia encompasses such basic issues as: (1) the necessity for bilateral lesions; (2) the intrahemispheric locus of damage; and (3) the roles of disconnection versus cortical damage. We examined three patients whose associative visual agnosia encompassed objects and printed words but spared faces. CAT scans revealed unilateral dominant occipitotemporal strokes. CAT scans of four previously reported cases with this same profile of associative agnosia were obtained. Dominant parahippocampal, fusiform and lingual gyri were the most extensively damaged cortical regions surveyed and were involved in all cases. Of white matter tracts surveyed, only temporal white matter including inferior longitudinal fasciculus was severely and universally involved. Splenium of the corpus callosum was frequently but not always involved. We conclude there is a form of associative visual agnosia with agnosia for objects and printed words but sparing face recognition which has a characteristic unilateral neuropathology. Damage or disconnection of dominant parahippocampal, fusiform and lingual gyri is the necessary and sufficient lesion.
Asunto(s)
Agnosia/diagnóstico , Aprendizaje por Asociación , Daño Encefálico Crónico/diagnóstico , Trastornos Cerebrovasculares/diagnóstico , Dislexia Adquirida/diagnóstico , Reconocimiento Visual de Modelos , Anciano , Agnosia/fisiopatología , Agnosia/psicología , Aprendizaje por Asociación/fisiología , Daño Encefálico Crónico/fisiopatología , Daño Encefálico Crónico/psicología , Mapeo Encefálico , Corteza Cerebral/fisiopatología , Trastornos Cerebrovasculares/fisiopatología , Trastornos Cerebrovasculares/psicología , Dominancia Cerebral/fisiología , Dislexia Adquirida/fisiopatología , Dislexia Adquirida/psicología , Femenino , Hipocampo/fisiopatología , Humanos , Masculino , Recuerdo Mental/fisiología , Persona de Mediana Edad , Pruebas Neuropsicológicas , Reconocimiento Visual de Modelos/fisiología , Tomografía Computarizada por Rayos XRESUMEN
We report a large sebaceous adenoma of the parotid gland. CT showed soft-tissue mass in the lateral portion of the adenoma corresponding to the histologic finding of proliferation of sebaceous glands and fibrous stromal tissue. There were numerous fatty cysts (-65Hu). Some of the cystic component ruptured, and CT demonstrated leaking of fatty material into the parapharyngeal space.
Asunto(s)
Adenoma/diagnóstico por imagen , Neoplasias de la Parótida/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Adenoma/patología , Adenoma/cirugía , Tejido Adiposo/patología , Adulto , División Celular/fisiología , Humanos , Masculino , Glándula Parótida/patología , Glándula Parótida/cirugía , Neoplasias de la Parótida/patología , Neoplasias de la Parótida/cirugía , Glándulas Sebáceas/patologíaRESUMEN
Glutathione (GSH) concentrations in human epidermoid carcinoma tissues were measured by high performance liquid chromatography. The mean glutathione content of 26 epidermoid carcinoma intratumor tissue specimens was 24.36 nmol/mg protein, which was significantly higher than that in adjacent non-tumor tissue parts (3.04 nmol/mg protein). The mean concentration found in normal oral mucosa was 4.80 nmol/mg protein. Tissue GSH levels were not correlated with the age of the patients or tumor size. Additionally, cellular GSH levels in nine different cell lines were found to spread over a wide range from 0.97 to 50.97 nmol/mg protein. Elevated GSH levels in cancer tissues were probably due to their abnormal proliferative activities. These results indicate that the glutathione level of oral tissues may be a useful marker for oral cancer, which is in agreement with findings from lung squamous cell carcinoma, cervical squamous cell carcinoma and other squamous cell carcinomas.