RESUMEN
RNA sequencing is an approach to transcriptomic profiling that enables the detection of differentially expressed genes in response to genetic mutation or experimental treatment, among other uses. Here we describe a method for the use of a customizable, user-friendly bioinformatic pipeline to identify differentially expressed genes in RNA sequencing data obtained from C. elegans, with attention to the improvement in reproducibility and accuracy of results.
Asunto(s)
Caenorhabditis elegans , Biología Computacional , Perfilación de la Expresión Génica , Análisis de Secuencia de ARN , Programas Informáticos , Flujo de Trabajo , Caenorhabditis elegans/genética , Animales , Biología Computacional/métodos , Análisis de Secuencia de ARN/métodos , Perfilación de la Expresión Génica/métodos , Transcriptoma , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Reproducibilidad de los ResultadosRESUMEN
Individuals with type 2 diabetes display metabolic abnormalities, such as hyperglycemia, increased free fatty acids, insulin resistance, and altered ceramide levels, that contribute to vascular dysfunctions and compromised oxygen delivery. Caenorhabditis elegans fed a glucose-supplemented diet or with altered ceramide metabolism, due to a hyl-2 mutation, are sensitive to oxygen deprivation (anoxia). Our experiments showed that the combination of these factors further decreased the anoxia survival. RNA-sequencing analysis was performed to assess how a glucose-supplemented diet and/or a hyl-2 mutation altered the transcriptome. Comparison analysis of transcripts associated with anoxia-sensitive animals [hyl-2(tm2031) mutation or a glucose diet] revealed 199 common transcripts encoded by genes with known or predicted functions involving innate immunity, cuticle function (collagens), or xenobiotic and endobiotic phase I and II detoxification system. Use of RNA interference (RNAi) to target gene products of the xenobiotic and endobiotic phase I and II detoxification system (UDP-glycosyltransferase and Cytochrome p450 genes; ugt-15, ugt-18, ugt-19, ugt-41, ugt-63, cyp-13A12, cyp-25A1, and cyp-33C8) increased anoxia survival in wild-type animals fed a standard diet. Anoxia sensitivity of the hyl-2(tm2031) animals was suppressed by RNAi of cyp-25A1 or cyp-33C8 genes. A glucose diet fed to the P0 hermaphrodite decreased the anoxia survival of its F1 embryos; however, the RNAi of ugt-63 and cyp-33C8 suppressed anoxia sensitivity. These studies provide evidence that the detoxification system impacts oxygen deprivation responses and that C. elegans can be used to model the conserved detoxification system.
Asunto(s)
Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Ceramidas/biosíntesis , Perfilación de la Expresión Génica , Glucosa/biosíntesis , Oxígeno/metabolismo , Transducción de Señal , Transcriptoma , Animales , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Análisis por Conglomerados , Biología Computacional/métodos , Técnicas de Silenciamiento del Gen , Hipoxia/genética , Hipoxia/metabolismo , Metabolismo de los Lípidos , Masculino , Fase I de la Desintoxicación Metabólica/genética , Fase II de la Desintoxicación Metabólica/genética , Mutación , Fenotipo , Xenobióticos/metabolismoRESUMEN
Diet is a central environmental factor that contributes to the phenotype and physiology of individuals. At the root of many human health issues is the excess of calorie intake relative to calorie expenditure. For example, the increasing amount of dietary sugars in the human diet is contributing to the rise of obesity and type 2 diabetes. Individuals with obesity and type 2 diabetes have compromised oxygen delivery, and thus it is of interest to investigate the impact a high-sugar diet has on oxygen deprivation responses. By utilizing the Caenorhabditis elegans genetic model system, which is anoxia tolerant, we determined that a glucose-supplemented diet negatively impacts responses to anoxia and that the insulin-like signaling pathway, through fatty acid and ceramide synthesis, modulates anoxia survival. Additionally, a glucose-supplemented diet alters lipid localization and initiates a positive chemotaxis response. Use of RNA-sequencing analysis to compare gene expression responses in animals fed either a standard or glucose-supplemented diet revealed that glucose impacts the expression of genes involved with multiple cellular processes including lipid and carbohydrate metabolism, stress responses, cell division, and extracellular functions. Several of the genes we identified show homology to human genes that are differentially regulated in response to obesity or type 2 diabetes, suggesting that there may be conserved gene expression responses between C. elegans fed a glucose-supplemented diet and a diabetic and/or obesity state observed in humans. These findings support the utility of the C. elegans model for understanding the molecular mechanisms regulating dietary-induced metabolic diseases.
Asunto(s)
Caenorhabditis elegans/metabolismo , Glucosa/metabolismo , Hipoxia/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , Insulina/metabolismo , Metabolismo de los Lípidos/genética , Transducción de Señal , Animales , Caenorhabditis elegans/genética , Metabolismo de los Hidratos de Carbono/genética , Ceramidas/biosíntesis , Dieta , Ácidos Grasos/biosíntesis , Perfilación de la Expresión Génica , Glucosa/administración & dosificación , Hipoxia/genéticaRESUMEN
Gamete cells pass on information to the next generation via DNA sequence and also through epigenetic mechanisms such as small RNAs, DNA methylation, or chromatin modifications. Caenorhabditis elegans is a genetic model system that an enormous number of talented researchers have used to understand biological phenomenon and develop molecular tools that have ultimately led to paradigm-shifting ideas in biology. Thus, this model is well poised to further investigate the molecular mechanisms involved with epigenetic modifications and transgenerational epigenetic inheritance. The strengths of this model system include a historical wealth of information regarding genetics, development, germline function, chromosome biology, and the regulation of gene expression. Using this system, one can investigate the mechanisms involved with how the germline passes on heritable epigenetic information to subsequent generations. Here, we highlight aspects about the biology of C. elegans that make it amenable to epigenetic studies, highlight some recent findings in the field of epigenetics, and comment on how this system would be beneficial for future biological studies involving epigenetic processes.
Asunto(s)
Envejecimiento/fisiología , Caenorhabditis elegans/genética , Ensamble y Desensamble de Cromatina/fisiología , Epigénesis Genética/fisiología , Patrón de Herencia/fisiología , Modelos Animales , Estrés Fisiológico/fisiología , Envejecimiento/genética , Animales , Epigénesis Genética/genética , Patrón de Herencia/genética , Membrana Nuclear/metabolismo , Estrés Fisiológico/genéticaRESUMEN
Caenorhabdits elegans has been used extensively in the study of stress resistance, which is facilitated by the transparency of the adult and embryo stages as well as by the availability of genetic mutants and transgenic strains expressing a myriad of fusion proteins(1-4). In addition, dynamic processes such as cell division can be viewed using fluorescently labeled reporter proteins. The study of mitosis can be facilitated through the use of time-lapse experiments in various systems including intact organisms; thus the early C. elegans embryo is well suited for this study. Presented here is a technique by which in vivo imaging of sub-cellular structures in response to anoxic (99.999% N2; <2 ppm O2) stress is possible using a simple gas flow through setup on a high-powered microscope. A microincubation chamber is used in conjunction with nitrogen gas flow through and a spinning disc confocal microscope to create a controlled environment in which animals can be imaged in vivo. Using GFP-tagged gamma tubulin and histone, the dynamics and arrest of cell division can be monitored before, during and after exposure to an oxygen-deprived environment. The results of this technique are high resolution, detailed videos and images of cellular structures within blastomeres of embryos exposed to oxygen deprivation.
Asunto(s)
Caenorhabditis elegans/metabolismo , Hipoxia/patología , Microscopía Confocal/métodos , Microscopía por Video/métodos , Animales , Animales Modificados Genéticamente , Caenorhabditis elegans/embriología , Caenorhabditis elegans/genética , Hipoxia/metabolismo , Procesamiento de Imagen Asistido por Computador , Microscopía Confocal/instrumentación , Microscopía por Video/instrumentaciónRESUMEN
Developing organisms require nutrients to support cell division vital for growth and development. An adaptation to stress, used by many organisms, is to reversibly enter an arrested state by reducing energy-requiring processes, such as development and cell division. This "wait it out" approach to survive stress until the environment is conductive for growth and development is used by many metazoans. Much is known about the molecular regulation of cell division, metazoan development and responses to environmental stress. However, how these biological processes intersect is less understood. Here, we review studies conducted in Caenorhabditis elegans that investigate how stresses such as oxygen deprivation (hypoxia and anoxia), exogenous chemicals or starvation affect cellular processes in the embryo, larvae or adult germline. Using C. elegans to identify how stress signals biological arrest can help in our understanding of evolutionary pressures as well as human health-related issues.
Asunto(s)
Caenorhabditis elegans/citología , Puntos de Control del Ciclo Celular , Oxígeno/metabolismo , Animales , Benomilo/farmacología , Blastómeros/citología , Blastómeros/metabolismo , Caenorhabditis elegans/efectos de los fármacos , Caenorhabditis elegans/metabolismo , Diferenciación Celular , División Celular , Embrión no Mamífero/citología , Embrión no Mamífero/efectos de los fármacos , Embrión no Mamífero/metabolismo , Desarrollo Embrionario , Células Germinativas/metabolismo , Larva/citología , Larva/efectos de los fármacos , Larva/metabolismo , Oocitos/citología , Oocitos/metabolismo , Estrés FisiológicoRESUMEN
Oxygen, an essential nutrient, is sensed by a multiple of cellular pathways that facilitate the responses to and survival of oxygen deprivation. The Caenorhabditis elegans embryo exposed to severe oxygen deprivation (anoxia) enters a state of suspended animation in which cell cycle progression reversibly arrests at specific stages. The mechanisms regulating interphase, prophase, or metaphase arrest in response to anoxia are not completely understood. Characteristics of arrested prophase blastomeres and oocytes are the alignment of condensed chromosomes at the nuclear periphery and an arrest of nuclear envelope breakdown. Notably, anoxia-induced prophase arrest is suppressed in mutant embryos lacking nucleoporin NPP-16/NUP50 function, indicating that this nucleoporin plays an important role in prophase arrest in wild-type embryos. Although the inactive form of cyclin-dependent kinase (CDK-1) is detected in wild-type-arrested prophase blastomeres, the inactive state is not detected in the anoxia exposed npp-16 mutant. Furthermore, we found that CDK-1 localizes near chromosomes in anoxia-exposed embryos. These data support the notion that NPP-16 and CDK-1 function to arrest prophase blastomeres in C. elegans embryos. The anoxia-induced shift of cells from an actively dividing state to an arrested state reveals a previously uncharacterized prophase checkpoint in the C. elegans embryo.