High-average-power mid-infrared ZnGeP2 optical parametric oscillator with a wavelength-dependent polarization rotator.

A wavelength-dependent polarization rotator is used to transform the orthogonal polarizations of the signal and idler of a near-degenerate type II KTP optical parametric oscillator (OPO) into a common polarization state. This common polarization allows a single ZnGeP2 OPO to fully utilize the 2 microm signal and idler KTP OPO outputs in a mid-IR conversion. The simple design of the wavelength-dependent polarization rotator yields a compact source that simultaneously generates four mid-JR wavelengths collinearly with a total mid-IR average power of 5.5 W at a >15 kHz pulse repetition rate.

Multiwatt mid-IR output from a Nd:YALO laser pumped intracavity KTA OPO.

We have achieved 4.1W of 3.5-micron output from a non-critically phasematched (NCPM), type II, KTiOAsO4 (KTA) optical parametric oscillator (OPO) pumped within the cavity of a Q-switched diode-pumped Nd: YALO laser operating at 10kHz. We adopted the simplest configuration with a compact diode-pumped Nd: YALO module pumping the singly resonant KTA OPO. Besides 4.1W of 3.5um, 10.9W of 1.5 micron and 11.3W of 1-micron radiation were obtained simultaneously.

Compact 21-W 2-mum intracavity optical parametric oscillator.

We report on an intracavity optical parametric oscillator (OPO) placed within a compact diode-pumped Nd:YALO laser cavity. This OPO utilizes a pair of KTP crystals, which are diffusion bonded together in a walk-off-compensated configuration. We have generated up to 21.4 W of 2-mum radiation, operating in a few-kilohertz range.

120-W continuous-wave diode-pumped Tm:YAG laser.

We present a 120-W cw diode-pumped Tm:YAG laser. The Tm:YAG rod is side pumped by three diode arrays whose radiation is coupled through compound parabolic concentrators. The maximum optical-to-optical conversion efficiency of the 2.02-mum laser output is 25.2%, with a slope efficiency of 31.2%.

Linearly polarized 100-W output from a diode-pumped Nd:YAlO laser.

We demonstrate a compact cw diode-pumped Nd:YAlO laser that can produce 100 W of power at 1079 nm and 18.3 W at 1341 nm. Lasing and nonlasing thermal lensing data are presented.

Multiwatt High-Repetition-Rate 2-microm Output from an Intracavity KTiOPO(4) Optical Parametric Oscillator.

We have obtained 6.5 W of 2-mum output from a near-degenerate, type II KTiOPO(4) (KTP) optical parametric oscillator (OPO) pumped within the cavity of a Q-switched diode-pumped Nd:YAG laser that operates at 3 kHz. We adopted the simplest configuration with a compact diode-pumped Nd:YAG module pumping the doubly resonant KTP OPO in its randomly polarized resonator with an acousto-optic Q switch. Attempts to increase the 2-mum output power by pumping this intracavity KTP OPO with a polarized laser beam by use of thermal birefringence compensation configurations are discussed.

High-efficiency mid-infrared ZnGeP2 optical parametric oscillator in a multimode-pumped tandem optical parametric oscillator.

We demonstrate a high-efficiency ZnGeP(2) optical parametric oscillator (OPO) pumped by another KTP OPO in a multimode-pumped tandem OPO configuration. The maximum optical-to-optical and slope efficiencies were 32% and 42.5%, respectively. Our setup also provides tunable multiband radiation in the 2.03-2.32-microm range and the 2.9-6.2-microm range simultaneously.

Influence of pulse sequence, polarity and amplitude on magnetic stimulation of human and porcine peripheral nerve.

1. Mammalian phrenic nerve, in a trough filled with saline, was excited by magnetic coil (MC)-induced stimuli at defined stimulation sites, including the negative-going first spatial derivative of the induced electric field along a straight nerve, at a bend in the nerve, and at a cut nerve ending. At all such sites, the largest amplitude response for a given stimulator output setting was elicited by an induced damped polyphasic pulse consisting of an initial quarter-cycle hyperpolarization followed by a half-cycle depolarization compared with a predominantly 'monophasic' quarter-cycle depolarization. 2. Simulation studies demonstrated that the increased efficacy of the induced quarter-cycle hyperpolarizing-half-cycle depolarizing polyphasic pulse was mainly attributed to the greater duration of the outward membrane current phase, resulting in a greater outward charge transfer afforded by the half-cycle (i.e. quarter-cycles 2 and 3). The advantage of a fast rising initial quarter-cycle depolarization was more than offset by the slower rising, but longer duration depolarizing half-cycle. 3. Simulation further revealed that the quarter-cycle hyperpolarization-half-cycle depolarization showed only a 2.6 % lowering of peak outward current and a 3.5 % lowering of outward charge transfer at threshold, compared with a half-cycle depolarization alone. Presumably, this slight increase in efficacy reflects modest reversal of Na+ inactivation by the very brief initial hyperpolarization. 4. In vitro, at low bath temperature, the nerve response to an initial quarter-cycle depolarization declined in amplitude as the second hyperpolarizing phase progressively increased in amplitude and duration. This 'pull-down' phenomenon nearly disappeared as the bath temperature approached 37 C. Possibly, at the reduced temperature, delay in generation of the action potential permitted the hyperpolarization phase to reduce excitation. 5. Pull-down was not observed in the thenar muscle responses to median nerve stimulation in a normal human at normal temperature. However, pull-down emerged when the median nerve was cooled by placing ice over the forearm. 6. In a nerve at subnormal temperature straddled with non-conducting inhomogeneities, polyphasic pulses of either polarity elicited the largest responses. This was also seen when stimulating distal median nerve at normal temperature. These results imply excitation by hyperpolarizing-depolarizing pulse sequences at two separate sites. Similarly, polyphasic pulses elicited the largest responses from nerve roots and motor cortex. 7. The pull-down phenomenon has a possible clinical application in detecting pathologically slowed activation of Na+ channels. The current direction of the polyphasic waveform may become a significant factor with the increasing use of repetitive magnetic stimulators which, for technical reasons, induce a cosine-shaped half-cycle, preceded and followed by quarter-cycles of opposite polarity.

Coupled tandem optical parametric oscillator (OPO): an OPO within an OPO.

We have demonstrated, for the first time to our knowledge, a coupled tandem optical parametric oscillator (OPO) configuration in which a second ZnGeP(2) OPO is placed within the resonator of the first, KTiOPO(4), OPO. A significant enhancement in the overall cascaded efficiency of this OPO compared with standard two-stage OPO's was observed. With a multimode Nd:YAG laser, an overall optical-to-optical efficiency (from 1.06 microm to the mid IR) of 5.2% was obtained from operating only ~1.4 times above oscillation threshold. The measured overall slope efficiency was attractively high at 35%. With a single set of mirrors we obtained a broad wavelength-tuning range from 2.7 to 8 microm.

Low-loss measurement in partially buried opticalwaveguideson glass with a plastic prism.

Surface and buried planar waveguides have been fabricated in glass microscope slides with purely thermal potassium and sodium ion-exchange techniques. We measured propagation loss as low as 0.08 dB/cm in the partially buried waveguides using an improved two-prism coupling method. The method includes a plastic prism and involves applying heat to soften the base of the outcoupling plastic prism so that the prism is temporarily in extremely close contact with the waveguide surface.

A kinase-deficient splice variant of the human JAK3 is expressed in hematopoietic and epithelial cancer cells.

Signal transduction of cytokine receptors is mediated by the JAK family of tyrosine kinases. Recently, the kinase partners for the interleukin (IL)-2 receptor have been identified as JAK1 and JAK3. In this study, we report the identification of splice variants that may modulate JAK3 signaling. Three splice variants were isolated from different mRNA sources: breast (B), spleen (S), and activated monocytes (M). Sequence analysis revealed that the splice variants contain identical NH2-terminal regions but diverge at the COOH termini. Analyses of expression of the JAK3 splice isoforms by reverse transcriptase-polymerase chain reaction on a panel of cell lines show splice preferences in different cell lines: the S-form is more commonly seen in hematopoietic lines, whereas the B- and M-forms are detected in cells both of hematopoietic and epithelial origins. Antibodies raised against peptides to the B-form splice variant confirmed that the 125-kDa JAK3B protein product is found abundantly in hematopoietic as well as epithelial cells, including primary breast cancers. The lack of subdomain XI in the tyrosine kinase core of the B-form JAK3 protein suggests that it is a defective kinase. This is supported by the lack of detected autokinase activity of the B-form JAK3. Intriguingly, both the S and B splice isoforms of JAK3 appear to co-immunoprecipitate with the IL-2 receptor from HUT-78 cell lysates. This and the presence of multiple COOH-terminal splice variants coexpressed in the same cells suggest that the JAK3 splice isoforms are functional in JAK3 signaling and may enrich the complexity of the intracellular responses functional in IL-2 or cytokine signaling.

Involvement of the Jak-3 Janus kinase in signalling by interleukins 2 and 4 in lymphoid and myeloid cells.

Many cytokines function through interaction with receptors of the cytokine receptor superfamily. Although lacking catalytic domains, cytokine receptors couple ligand binding to induction of protein tyrosine phosphorylation. Recent studies have shown that one or more of the Janus kinase family members (Jaks) associate with cytokine receptors and are tyrosine phosphorylated and activated following ligand binding. Here we describe a new Jak family kinase, Jak-3, and demonstrate that Jak-3, and to a lesser extent Jak-1, are tyrosine phosphorylated and Jak-3 is activated in the responses to interleukin-2 and interleukin-4 in T cells and myeloid cells. Jak-3 activation requires the serine-rich, membrane-proximal domain of the interleukin-2 receptor beta-chain, but does not require the acidic domain that is required for association and activation of Src family kinases.

Changes in nerve conduction and Pi/PCr ratio during denervation-reinnervation of the gastrocsoleus muscles of rats.

The purpose of this investigation was to study the changes in nerve conduction and phosphate metabolites of the gastrocsoleus muscles of rats during denervation-reinnervation. Sixteen male Sprague-Dawley rats underwent unilateral crush-denervation of the left sciatic nerves at the sciatic notch. Six rats were used for measurement of motor conduction latency and action potential amplitude of the gastrocsoleus muscle by stimulating the sciatic nerve at one, two and eight weeks after nerve crush. The other ten rats were designated for evaluation of the ratio of inorganic phosphorous (Pi) to phosphocreatine (PCr) by a 31P-phosphoenergetic spectrometer at two weeks and eight weeks after nerve crush. None of the sciatic nerves showed conduction to the gastrocsoleus at one or two weeks after nerve crush. At eight weeks postcrush, the motor conduction latency returned to within normal limits, whereas the action potential amplitude was only 55% of the normal. For the eight-week period of study, the Pi/PCr ratio of the normal control muscles ranged between 0.09 +/- 0.02 and 0.11 +/- 0.02 (mean +/- SD). The denervated muscles showed an increase of Pi/PCr ratio by 54% at two weeks postcrush, compared to the respective contralateral control sides. The ratios returned to the normal value by eight weeks postcrush. In summary, these data suggested that the metabolic recovery of the crush-denervated muscle followed the same pattern as the parameters of nerve conduction.

The course of giardiavirus infection in the Giardia lamblia trophozoites.

The subcellular distribution of Giardia lamblia virus RNA in infected G. lamblia trophozoites was examined by in situ hybridization using biotinylated DNA probe and riboprobe. In G. lamblia Portland I strain, which is chronically infected by G. lamblia viruses, the viral RNA was detected in the cytoplasm as well as in the twin nuclei. When riboprobe was used to examine the course of virus infection in WB strain, accumulation of viral RNA was detected only in the cytoplasm prior to the first 72 hr of infection. Using DNA probe, further accumulation of viral RNA in increasing number of cells occurred after the 72nd hr of infection, with the RNA found in both the cytoplasm and nuclei. Eventually, the cell nuclei showed damaged morphology that deteriorated rapidly toward the final stage of infection. These observations indicate that early phase of viral RNA replication may take place in the cytoplasm of infected G. lamblia, but the nuclei are also involved during the late phase of viral replication.

Measurement of the electric field induced into inhomogeneous volume conductors by magnetic coils: application to human spinal neurogeometry.

We measured the electric fields induced by round and figure "8" magnetic coils (MCs) in homogeneous and inhomogeneous volume conductors. In homogeneous media, the round MC held tangential (i.e., flat) to the volume conductor induced an annular electric field. When the round MC was held on-edge (i.e., orthogonal) to the volume conductor, the induced electric field consisted of two loops mainly parallel to the surface of the volume conductor and which approximated each other directly under the contacting edge of the MC. The tangentially oriented figure "8" MC similarly induced two electric field loops which approximated one another maximally under the region of the junction in its long axis. In a complex inhomogeneous volume conductor, such as a segment of human cervical-thoracic vertebral spine located eccentrically within a large cylindrical tank and submerged in isotonic saline, the direction of electric fields within the spinal canal and across the intervertebral neuroforamina was similar to that observed in the homogeneous volume conductor. However, in and near a single neuroforamen, the electric field and especially its first spatial derivative were markedly elevated compared to that recorded within the long central axis of the vertebral canal. Motor unit and compound muscle action potentials elicited in limb muscles by MC stimulation of human cervical spine confirmed predictions derived from the physical model. The predictions included: (1) absence of spinal cord stimulation compared to relative ease of nerve root stimulation by current that is most likely concentrated at the neuroforamina. When stimulating current is directed towards the periphery, the most likely low threshold site of stimulation is inferred to be just distal to the neuroforamina. It is emphasized that with supramaximal stimulation, more distal sites of excitation may occur; (2) invariant latency shifts at threshold intensities when moving the MC along the rostrocaudal axis of the cervical vertebral column; (3) significant effect (on motor unit activation thresholds) of the direction of induced current flow across the neuroforamina; (4) reduced stimulation when the targeted nerve roots are close to the null point of the electric field, i.e., between locations of high electric field intensity, of opposite polarity; and (5) relatively focal nerve root stimulation by the junction of a transversely orientated figure "8" MC, i.e., parallel to the nerve roots.

High prevalence of human papillomavirus infection and possible association with betel quid chewing and smoking in oral epidermoid carcinomas in Taiwan.

Seventeen oral epidermoid carcinomas, three oral papillomas, and 17 normal gingival tissues were tested for the presence of human papillomavirus (HPV) types 6, 11, 16, and 18 sequences by Southern blot hybridization. Episomal HPV-16 sequences in various amounts were detected in 76.4% of the oral carcinomas and in all three cases of papilloma. However, only one of the 17 normal tissues was HPV positive with an unknown type. None of the samples contained HPV-6, -11, or -18 sequences. Examination of the habits of the patients showed that 59% of the patients were betel quid chewers and 82% were smokers. Thus, the concurrent incidence of HPV infection and betal quid chewing and/or smoking habits in oral carcinoma patients observed in Taiwan is consistent with the view that both viral and chemical factors may be involved in the process of carcinogenesis.

The appearance of rapidly flowing blood on magnetic resonance images.

The appearance of rapidly flowing blood on imaging (MRI) was evaluated using flow phantoms and dye infusion experiments. Laminar flow can be maintained at high velocities in small-diameter vessels. Under such conditions, flow-related enhancement may be observed several slices into a multislice imaging volume. Decreasing cross-sectional area of the unsaturated protons in the midstream is noted on slices further removed from the entry surface. As the velocity increases, turbulence occurs. The increased random motion of the protons causes loss of intensity on the first-echo image, although rephasing with increased intensity can be noted on the second-echo image. The flow pattern of a simple intraluminal obstruction is demonstrated by MRI and dye infusion experiments. Rephasing is noted within the eddy downstream from the obstruction. Clinical examples of the phantom findings are shown and applications are discussed.

Adult Niemann-Pick disease--a case report.

A case is presented of the rare adult form of Niemann-Pick disease occurring in a Cantonese. The diagnosis was verified by biochemical analysis of the affected tissues.