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In the context of China's transportation sector, which has faced escalating challenges in carbon emissions, this study delves into the intricate nexus between sustainable finance strategies and the imperative of achieving carbon neutrality. Spanning the years 2010-2022 across 30 provinces of China and employing a rigorous Panel Model methodology, our research sets out to achieve several pivotal objectives. These include assessing the tangible impact of sustainable finance initiatives on curtailing carbon emissions within the transportation domain, discerning the pivotal drivers that influence the trajectory of carbon neutrality endeavors, and critically evaluating the efficacy of policy interventions aimed at fostering sustainability. Our findings unearth a compelling narrative. Firstly, we observe a discernible positive correlation between the implementation of sustainable finance mechanisms-such as green bonds, sustainable investment portfolios, and innovative financial instruments-and the tangible reduction of carbon emissions within the transportation sector. Secondly, our analysis underscores the indispensable role of key drivers, ranging from technological advancements and regulatory frameworks to evolving consumer behavior and public consciousness, in steering the course towards carbon neutrality. Thirdly, our research underscores the pivotal impact of targeted policy interventions, emphasizing the efficacy of measures aimed at incentivizing sustainable practices, fostering stakeholder collaborations, and bolstering industry-wide accountability frameworks. In light of these insights, our study advocates for a nuanced policy landscape characterized by a multifaceted approach. By aligning financial incentives with sustainability goals, fostering technological innovation, and fostering robust regulatory frameworks, policymakers can catalyze a paradigm shift towards carbon neutrality in the transportation sector.
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In the context of diabetes, endothelial cells frequently exhibit compromised intercellular junctions and accelerated cellular senescence simultaneously. The precise mechanisms underlying these issues and the identification of effective treatments remain largely undefined. Our findings reveal that human umbilical vein endothelial cells (HUVECs) can counteract senescence and uphold the integrity of intercellular junctions under mildly to moderately elevated glucose levels (10 mM and 15 mM) via two primary mechanisms: i) The acetylation of NRF2 at lysine residues K56, K68, and K52 prevents its ubiquitination, enhancing the transcription of antioxidant genes GST, SOD1, and GPX1. This activity diminishes cytoplasmic oxidative stress, thereby mitigating endothelial cell senescence. ii) The interaction between the Neh2 domain of NRF2 and the PAS-B domain of HIF-2α within the nucleus curtails the attachment of HIF-2α to the NOX4/p22phox promoter. This action lessens oxidative stress near the cell membrane, maintaining intercellular junctions by safeguarding the disulfide bonds in occludin and E-cadherin from disruption. However, these protective strategies prove insufficient under severe hyperglycemic conditions (25 mM). Further investigation has identified Oltipraz, an activator of NRF2, as also promoting the degradation of HIF-2α. Through its simultaneous modulation of NRF2 and HIF-2α, Oltipraz significantly reduces cellular senescence and prevents the deterioration of intercellular junctions in HUVECs subjected to high glucose concentrations (25 mM). Our research positions Oltipraz as a promising therapeutic candidate for mitigating diabetes-induced vascular endothelial damage, potentially offering benefits against diabetes-related atherosclerosis and valvular calcification.
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Senescencia Celular , Células Endoteliales de la Vena Umbilical Humana , Uniones Intercelulares , Factor 2 Relacionado con NF-E2 , Transducción de Señal , Humanos , Factor 2 Relacionado con NF-E2/metabolismo , Senescencia Celular/fisiología , Células Endoteliales de la Vena Umbilical Humana/metabolismo , Uniones Intercelulares/metabolismo , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Estrés Oxidativo , Diabetes Mellitus/metabolismo , Glucosa/metabolismoRESUMEN
BACKGROUND: Rural African people living with HIV face significant challenges in entering and remaining in HIV care. In rural Uganda, for example, there is a threefold higher prevalence of HIV compared to the national average and lower engagement throughout the HIV continuum of care. There is an urgent need for appropriate interventions to improve entry and retention in HIV care for rural Ugandans with HIV. Though many adults living with HIV in rural areas prioritize seeking care services from traditional healers over formal clinical services, healers have not been integrated into HIV care programs. The Omuyambi trial is investigating the effectiveness of psychosocial support delivered by traditional healers as an adjunct to standard HIV care versus standard clinic-based HIV care alone. Additionally, we are evaluating the implementation process and outcomes, following the Consolidated Framework for Implementation Research. METHODS: This cluster randomized hybrid type 1 effectiveness-implementation trial will be conducted among 44 traditional healers in two districts of southwestern Uganda. Healers were randomized 1:1 into study arms, where healers in the intervention arm will provide 12 months of psychosocial support to adults with unsuppressed HIV viral loads receiving care at their practices. A total of 650 adults with unsuppressed HIV viral loads will be recruited from healer clusters in the Mbarara and Rwampara districts. The primary study outcome is HIV viral load measured at 12 months after enrollment, which will be analyzed by intention-to-treat. Secondary clinical outcome measures include (re)initiation of HIV care, antiretroviral therapy adherence, and retention in care. The implementation outcomes of adoption, fidelity, appropriateness, and acceptability will be evaluated through key informant interviews and structured surveys at baseline, 3, 9, 12, and 24 months. Sustainability will be measured through HIV viral load measurements at 24 months following enrollment. DISCUSSION: The Omuyambi trial is evaluating an approach that could improve HIV outcomes by incorporating previously overlooked community lay supporters into the HIV cascade of care. These findings could provide effectiveness and implementation evidence to guide the development of policies and programs aimed at improving HIV outcomes in rural Uganda and other countries where healers play an essential role in community health. TRIAL REGISTRATION: ClinicalTrials.gov NCT05943548. Registered on July 5, 2023. The current protocol version is 4.0 (September 29, 2023).
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Infecciones por VIH , Ensayos Clínicos Controlados Aleatorios como Asunto , Carga Viral , Humanos , Infecciones por VIH/tratamiento farmacológico , Infecciones por VIH/diagnóstico , Uganda/epidemiología , Medicinas Tradicionales Africanas/métodos , Fármacos Anti-VIH/uso terapéutico , Resultado del Tratamiento , Servicios de Salud Rural , Adulto , Apoyo Social , Población Rural , Factores de Tiempo , Femenino , Masculino , Practicantes de la Medicina TradicionalRESUMEN
Vitiligo is a common skin depigmentation disorder characterized by the patchy loss of skin color. Nowadays, it is recognized as being correlated with multiple genetic factors as well as the psychological conditions of individuals. Long noncoding RNAs have been reported to underlie the pathogenesis of vitiligo; however, the role of long noncoding RNAs in the stress-related depigmentation process remains largely unknown. In this study, the inhibition of melanocyte function was observed in C57BL/6J mice modeled through chronic restraint stress. Furthermore, downregulation of the expression of the long noncoding RNAs Mir17hg was identified using RNA sequencing. The regulatory role of Mir17hg in melanogenesis was also investigated in melanocytes and zebrafish embryos through overexpression or knockdown. Finally, TGFß receptor 2 was shown to be a downstream target in Mir17hg-mediated melanogenesis regulation, in which the classical TGFß/SMAD signaling cascade and the PI3K/AKT/mTOR signaling cascade play important roles. In conclusion, our results revealed an important regulatory role of Mir17hg in melanogenesis through inhibition of TGFßR2, which can provide a potential therapeutic target for treating skin depigmentation disorders.
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ARN Largo no Codificante , Vitíligo , Animales , Ratones , Melanocitos/metabolismo , Melanogénesis , Ratones Endogámicos C57BL , Fosfatidilinositol 3-Quinasas/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Estrés Psicológico/genética , Factor de Crecimiento Transformador beta/metabolismo , Vitíligo/patología , Pez Cebra/metabolismoRESUMEN
We extend our recently proposed theoretical framework for estimating cavity-modified equilibrium Fermi's golden rule (FGR) rate constants beyond the single cavity mode case to cases where the molecular system is coupled to multiple cavity modes. We show that the cumulative effect of simultaneous coupling to multiple modes can enhance FGR rate constants by orders of magnitude relative to the single mode case. We also present an analysis of the conditions necessary for maximizing this effect in the Marcus limit of FGR-based rate theory.
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Vitiligo is a common primary, limited or generalized skin depigmentation disorder. Its pathogenesis is complex, multifactorial and unclear. For this reason, few animal models can simulate the onset of vitiligo, and studies of drug interventions are limited. Studies have found that there may be a pathophysiological connection between mental factors and the development of vitiligo. At present, the construction methods of the vitiligo model mainly include chemical induction and autoimmune induction against melanocytes. Mental factors are not taken into account in existing models. Therefore, in this study, mental inducement was added to the monobenzone (MBEH)-induced vitiligo model. We determined that chronic unpredictable mild stress (CUMS) inhibited the melanogenesis of skin. MBEH inhibited melanin production without affecting the behavioral state of mice, but mice in the MBEH combined with CUMS (MC) group were depressed and demonstrated increased depigmentation of the skin. Further analysis of metabolic differences showed that all three models altered the metabolic profile of the skin. In summary, we successfully constructed a vitiligo mouse model induced by MBEH combined with CUMS, which may be better used in the evaluation and study of vitiligo drugs.
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Hipopigmentación , Vitíligo , Animales , Ratones , Vitíligo/patología , Hidroquinonas/farmacología , Piel/metabolismo , Melanocitos , Modelos Animales de EnfermedadRESUMEN
The field of nanozymes is developing rapidly. In particular, glutathione peroxidase (GPx)-like nanozymes, which catalytically reduce H2O2/organic hydroperoxides to H2O/alcohols, have attracted considerable attention. GPx-like nanozymes are powerful antioxidant enzymes known to combat oxidative stress. They have broad applications, including cytoprotection, anti-inflammation, neuroprotection, tumor therapy, and anti-aging. Although much progress has been made, GPx-like nanozymes have not been well discussed or fully reviewed as other nanozymes. This review aims to summarize recent advances on GPx-like nanozymes from the vantage point of mechanism, classification, and bioapplication. Future prospects for advancing their design and application are also discussed.
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Peróxido de Hidrógeno , Nanoestructuras , Glutatión Peroxidasa , Catálisis , Antioxidantes , Estrés OxidativoRESUMEN
Quantum master equations (QMEs) provide a general framework for describing electronic dynamics within a complex molecular system. Off-diagonal QMEs (OD-QMEs) correspond to a family of QMEs that describe the electronic dynamics in the interaction picture based on treating the off-diagonal coupling terms between electronic states as a small perturbation within the framework of second-order perturbation theory. The fact that OD-QMEs are given in terms of the interaction picture makes it non-trivial to obtain Schrödinger picture electronic coherences from them. A key experimental quantity that relies on the ability to obtain accurate Schrödinger picture electronic coherences is the absorption spectrum. In this paper, we propose using a recently introduced procedure for extracting Schrödinger picture electronic coherences from interaction picture inputs to calculate electronic absorption spectra from the electronic dynamics generated by OD-QMEs. The accuracy of the absorption spectra obtained this way is studied in the context of a biexciton benchmark model, by comparing spectra calculated based on time-local and time-nonlocal OD-QMEs to spectra calculated based on a Redfield-type QME and the non-perturbative and quantum-mechanically exact hierarchical equations of motion method.
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The 5-HT2A serotonin receptor (HTR2A) has been reported to be involved in the serotonin- or serotonin receptor 2A agonist-induced melanogenesis in human melanoma cells. However, the molecular mechanisms underlying HTR2A in regulating melanogenesis remain poorly understood. In this research, cultured mouse melanoma cell line B16F10, human skin, and zebrafish embryos were used to elucidate the downstream signaling of HTR2A in regulating melanogenesis and to verify the potential application of HTR2A in the treatment of pigment-associated cutaneous diseases. We demonstrated that HTR2A antagonists (AT1015 and ketanserin) attenuated the melanogenesis induction of serotonin in both mouse melanoma cells and zebrafish embryos. The agonists of HTR2A (DOI and TCB-2) increased melanin synthesis and transfer in B16F10 cells, human skin tissue, and zebrafish embryos. Furthermore, the HTR2A agonists increased the expression of proteins related to melanosome organization and melanocyte dendrites to facilitate the melanocyte migration and melanosome transport. HTR2A antagonists and genetic knockout of zebrafish htr2aa (the homologue of mammalian HTR2A gene) were also used to clarify that HTR2A mediates serotonin and DOI in regulating melanogenesis. Finally, through small scale screening of the candidate downstream pathway, we demonstrated that HTR2A mediates the melanogenesis induction of its ligands by activating the PKA/CREB signaling pathway. In this research, we further confirmed that HTR2A is a crucial protein to mediate melanocyte function. Meanwhile, this research supports that HTR2A could be designed as a drug target for the development of chemicals to treat cutaneous diseases with melanocytes or melanogenesis abnormality.
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Melaninas , Melanoma , Animales , Línea Celular Tumoral , Mamíferos/metabolismo , Melaninas/metabolismo , Melanocitos/metabolismo , Melanoma/metabolismo , Ratones , Serotonina/metabolismo , Serotonina/farmacología , Transducción de Señal , Pez Cebra/metabolismoRESUMEN
We show that combining the linearized semiclasscial approximation with Fermi's golden rule (FGR) rate theory gives rise to a general-purpose cost-effective and scalable computational framework that can accurately capture the cavity-induced rate enhancement of charge transfer reactions that occurs when the molecular system is placed inside a microcavity. Both partial linearization with respect to the nuclear and photonic degrees of freedom and full linerization with respect to nuclear, photonic, and electronic degrees of freedom (the latter within the mapping Hamiltonian approach) are shown to be highly accurate, provided that the Wigner transforms of the product (WoP) of operators at the initial time is not replaced by the product of their Wigner transforms. We also show that the partial linearization method yields the quantum-mechanically exact cavity-modified FGR rate constant for a model system in which the donor and acceptor potential energy surfaces are harmonic and identical except for a shift in the equilibrium energy and geometry, if WoP is applied.
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Teoría CuánticaRESUMEN
Quantum master equations provide a general framework for describing the dynamics of electronic observables within a complex molecular system. One particular family of such equations is based on treating the off-diagonal coupling terms between electronic states as a small perturbation within the framework of second-order perturbation theory. In this paper, we show how different choices of projection operators, as well as whether one starts out with the time-convolution or the time-convolutionless forms of the generalized quantum master equation, give rise to four different types of such off-diagonal quantum master equations (OD-QMEs), namely, time-convolution and time-convolutionless versions of a Pauli-type OD-QME for only the electronic populations and an OD-QME for the full electronic density matrix (including both electronic populations and coherences). The fact that those OD-QMEs are given in terms of the interaction picture makes it non-trivial to obtain Schrödinger picture electronic coherences from them. To address this, we also extend a procedure for extracting Schrödinger picture electronic coherences from interaction picture populations recently introduced by Trushechkin in the context of time-convolutionless Pauli-type OD-QME to the other three types of OD-QMEs. The performance of the aforementioned four types of OD-QMEs is explored in the context of the Garg-Onuchic-Ambegaokar benchmark model for charge transfer in the condensed phase across a relatively wide parameter range. The results show that time-convolution OD-QMEs can be significantly more accurate than their time-convolutionless counterparts, particularly in the case of Pauli-type OD-QMEs, and that rather accurate Schrödinger picture coherences can be obtained from interaction picture electronic inputs.
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Depression is the most frequent affective disorder and is the leading cause of disability worldwide. In order to screen antidepressants and explore molecular mechanisms, a variety of animal models were used in experiments, but there is no reliable high-throughput screening method. Zebrafish is a common model organism for mental illness such as depression. In our research, we established chronic unpredictable mild stress (CUMS) models in C57BL/6 mice and zebrafish; the similarities in behavior and pathology suggest that zebrafish can replace rodents as high-throughput screening organisms. Stress mice (ip., 1 mg/kg/d, 3 days) and zebrafish (10 mg/L, 20 min) were treated with reserpine. As a result, reserpine caused depression-like behavior in mice, which was consistent with the results of the CUMS mice model. Additionally, reserpine reduced the locomotor ability and exploratory behavior of zebrafish, which was consistent with the results of the CUMS zebrafish model. Further analysis of the metabolic differences showed that the reserpine-induced zebrafish depression model was similar to the reserpine mice model and the CUMS mice model in the tyrosine metabolism pathway. The above results showed that the reserpine-induced depression zebrafish model was similar to the CUMS model from phenotype to internal metabolic changes and can replace the CUMS model for antidepressants screening. Moreover, the results from this model were obtained in a short time, which can shorten the cycle of drug screening and achieve high-throughput screening. Therefore, we believe it is a reliable high-throughput screening model.
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Antidepresivos/farmacología , Conducta Animal/efectos de los fármacos , Depresión , Conducta Exploratoria/efectos de los fármacos , Locomoción/efectos de los fármacos , Estrés Psicológico , Animales , Depresión/inducido químicamente , Depresión/tratamiento farmacológico , Depresión/fisiopatología , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Masculino , Ratones , Reserpina/efectos adversos , Reserpina/farmacología , Estrés Psicológico/inducido químicamente , Estrés Psicológico/tratamiento farmacológico , Estrés Psicológico/fisiopatología , Pez CebraRESUMEN
To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment of skin depigmentation disorder, we have made use of Vernonia anthelmintica (L.) Willd., a traditional Chinese herbal medicine that has been proven to be effective in treating vitiligo. Here, we report that the extract of Vernonia anthelmintica (L.) Willd. effectively enhances melanogenesis responses in B16F10. In its compound library, we found three ingredients (butin, caffeic acid and luteolin) also have the activity of promoting melanogenesis in vivo and in vitro. They can reduce the accumulation of ROS induced by hydrogen peroxide and inflammatory response induced by sublethal concentrations of copper sulfate in wild type and green fluorescent protein (GFP)-labeled leukocytes zebrafish larvae. The overall objective of the present study aims to identify which compatibility proportions of the medicines may be more effective in promoting pigmentation. We utilized the D-optimal response surface methodology to optimize the ratio among three molecules. Combining three indicators of promoting melanogenesis, anti-inflammatory and antioxidant capacities, we get the best effect of butin, caffeic acid and luteolin at the ratio (butin:caffeic acid:luteolin = 7.38:28.30:64.32) on zebrafish. Moreover, the effect of melanin content recovery in the best combination is stronger than that of the monomer, which suggests that the three compounds have a synergistic effect on inducing melanogenesis. After simply verifying the result, we performed in situ hybridization on whole-mount zebrafish embryos to further explore the effects of multi-drugs combination on the proliferation and differentiation of melanocytes and the expression of genes (tyr, mitfa, dct, kit) related to melanin synthesis. In conclusion, the above three compatible compounds can significantly enhance melanogenesis and improve depigmentation in vivo.
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Melaninas/biosíntesis , Vernonia/química , Animales , Antiinflamatorios/farmacología , Benzopiranos/farmacología , Ácidos Cafeicos/farmacología , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Modelos Animales de Enfermedad , Quimioterapia Combinada , Regulación de la Expresión Génica/efectos de los fármacos , Inflamación/patología , Luteolina/farmacología , Melaninas/genética , Melanoma Experimental/patología , Fenotipo , Especies Reactivas de Oxígeno/metabolismo , Reproducibilidad de los Resultados , Pez Cebra/genéticaRESUMEN
In this paper, we compare the ability of different quasiclassical mapping Hamiltonian methods to accurately simulate the absorption spectra of multiexcitonic molecular systems. Two distinctly different approaches for simulating the absorption spectra are considered: (1) a perturbative approach, which relies on the first-order perturbation theory with respect to the field-matter interaction; (2) a nonperturbative approach, which mimics the experimental measurement of the absorption spectra from the free-induction decay that follows a short laser pulse. The methods compared are several variations of the linearized semiclassical (LSC) method, the symmetrical quasiclassical (SQC) method, and the mean-field (Ehrenfest) method. The comparison is performed in the context of a biexcitonic model and a seven-excitonic model of the Fenna-Matthews-Olson (FMO) complex. The accuracy of the various methods is tested by comparing their predictions to the quantum-mechanically exact results obtained via the hierarchy of the equations of motion (HEOM) method, as well as to the results based on the Redfield quantum master equation. The results show that the LSC-based quasiclassical mapping Hamiltonian methods can yield the accurate and robust absorption spectra in the high-temperature and/or slow-bath limit, where the nuclear degrees of freedom can be treated as classical.
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In this work, we investigate the ability of different quasiclassical mapping Hamiltonian methods to simulate the dynamics of electronic transitions through conical intersections. The analysis is carried out within the framework of the linear vibronic coupling (LVC) model. The methods compared are the Ehrenfest method, the symmetrical quasiclassical method, and several variations of the linearized semiclassical (LSC) method, including ones that are based on the recently introduced modified representation of the identity operator. The accuracy of the various methods is tested by comparing their predictions to quantum-mechanically exact results obtained via the multiconfiguration time-dependent Hartree (MCTDH) method. The LVC model is found to be a nontrivial benchmark model that can differentiate between different approximate methods based on their accuracy better than previously used benchmark models. In the three systems studied, two of the LSC methods are found to provide the most accurate description of electronic transitions through conical intersections.
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The tumor microenvironment has been recently recognized as a critical contributor to cancer progression and anticancer therapy-resistance. Cyclophosphamide (CTX) is a cytotoxic agent commonly used in clinics for the treatment of cancer. Previous reports demonstrated that CTX given at low continuous doses, known as metronomic schedule, mainly targets endothelial cells and circulating Tregs with unknown mechanisms. Here, we investigated the antitumor activity of two different metronomic schedules of CTX along with their corresponding MTD regimen and further explored their effect on immune function and tumor microenvironment. Toxicity evaluation was monitored by overall survival rate, weight loss, and histopathological analysis. A nude mouse model of Lewis lung cancer was established to assess the anti-metastatic effects of CTX in vivo. CD4+, CD8+, and CD4+CD25+FoxP3 T cells were selected by flow cytometry analysis. Low and continuous administration of CTX was able to restore immune function via increase of CD4+/CD8+ T cells and depletion of T regulatory cells, not only in circulatory and splenic compartments, but also at the tumor site. Low-dose CTX also reduced myofibroblasts, accompanied with an increased level of E-cadherin and low N-cadherin, both in the primary tumor and lung through the TGF-ß pathway by the downregulated expression of TGF-ß receptor 2. Our data may indicate that several other molecular mechanisms of CTX for tumor may be involved in metronomic chemotherapy, besides targeting angiogenesis and regulatory T cells.
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Antineoplásicos Alquilantes/administración & dosificación , Carcinoma Pulmonar de Lewis/tratamiento farmacológico , Ciclofosfamida/administración & dosificación , Receptor Tipo II de Factor de Crecimiento Transformador beta/metabolismo , Factor de Crecimiento Transformador beta/metabolismo , Administración Metronómica , Animales , Antineoplásicos Alquilantes/farmacología , Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD8-positivos/metabolismo , Carcinoma Pulmonar de Lewis/metabolismo , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Ciclofosfamida/farmacología , Regulación hacia Abajo , Ratones , Ratones Desnudos , Transducción de Señal/efectos de los fármacos , Microambiente Tumoral/efectos de los fármacosRESUMEN
A bioactive component, 2',3,4,4'-tetrahydrochalcone (RY3-a) was first isolated from Vernohia anthelmintica (L.) willd seeds, and a set of its analogs, RY3-a-1-RY3-a-15 and RY3-c were designed and synthesized. Biological activity assays showed that RY3-c exhibited better melanogenesis and antioxidant activity and lower toxicity in comparison with RY3-a and butin. Further study tests showed that RY3-c exhibited better melanogenesis activity compared with the positive control 8-methoxypsoralan (8-MOP) in a vitiligo mouse model, suggesting that RY3-c is a good candidate antivitiligo agent. Mechanistic studies showed that RY3-c could repair cell damage induced by excessive oxidative stress and may exert melanin synthesis activity in the mouse melanoma B16F10 cell line by activating the mitogen-activated protein kinase (MAPK) pathway and the upregulation of c-kit.
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Productos Biológicos/farmacología , Chalcona/farmacología , Diseño de Fármacos , Vernonia/química , Vitíligo/tratamiento farmacológico , Animales , Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Chalcona/análogos & derivados , Chalcona/química , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos C57BL , Estructura Molecular , Estrés Oxidativo/efectos de los fármacos , Relación Estructura-Actividad , Vitíligo/metabolismo , Vitíligo/patología , Pez CebraRESUMEN
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.