COMPARATIVE EFFECT OF FUCOIDAN FROM ALGA FUCUS VESICULOSUS AND ITS FRACTIONS, OBTAINED BY ANION-EXCHANGE CHROMATOGRAPHY, ON CELL LINES HeLa G-63, Hep G2 AND CHANG LIVER.

The aim of the present study was to compare the effect of sulfated fucopolysaccharides isolated from Fucus vesiculosus on cell lines HeLa G-63, Hep G2 and Chang liver. Native fucoidan F3 and two fractions (F3-0.5 and F3-1), obtained by anion-exchange chromatography, were analyzed using chemical methods and IR-spectroscopy. Their action on cells demonstrates that F3 and F3-1, characterized by higher content of sulfates, preferable location of sulfo-groups at C4 atom of fucose residue and low content of uronic acids, inhibit cell proliferation more efficiently. Human liver tumor cells Hep G2 appeared to be the most sensitive to fucoidan treatment whereas nonmalignant human cells Chang liver were the least sensitive.

[EFFECT OF FUCOIDANS ISOLATED FROM SEAWEEDS LAMINARIA DIGITATA AND FUCUS VESICULOSUS ON CELL LINES HELA G-63, ECV 304 AND PC 12].

The aim of the research was to investigate cytotoxicity of fucoidans on mammals cells. Three different samples of fucoidans were isolated from mechanically grounded brown algae Laminaria digitata and Fucus ve- siculosus. The sample F2 that differed from the others by higher sulfatation level and suppression of HeLa G-63 line culture growth was taken for further study in cell lines HeLa G-63, ECV 304 and PC 12. We have shown that fucoidan preparation F2 inhibits proliferation and induces cell death in a dose- and time-dependent manner for all investigated cell lines. Neuroendocrine tumor rat cell line PC 12 appeared to be the most sensitive to fucoidan treatment whereas endothelial human cells ECV 304 were the least sensitive.

[Experimental validation of using ozonized physiological solutions in intraocular infection]. / Eksperimental'noe obosnovanie tselesoobraznosti primeneniia ozonirovannykh fiziologicheskikh rastvorov pri vnutriglaznoi infektsii.

The study was aimed at assessing the efficacy of ozonized normal salines in experimental endophthalmitis. Experiments demonstrated that, as the control group developed grave endophthalmitis and the clinical picture deteriorated by the end of experiment, eventuating in melting of tissues both in the anterior and posterior segments of the eye, in experimental animals, to which ozonized normal saline was administered directly after infection, endophthalmitis either did not develop at all or was less severe. The anterior segment of the eyeball was not involved in the infectious process, and the changes in the vitreous body were not so grave as in the controls, and by the end of experiment the inflammation ceased completely. By the end of the infectious process in the experimental group the majority of tissue structures of the posterior pole of the eyeball, including the retina, were retained. After administration of ozonized normal saline to animals with full-blood endophthalmitis the symptoms of the inflammatory process abated and the disease took a more favorable course than in control. Hence, experiments demonstrated that even a single intravitreal instillation of ozonized solution during an infectious process brings about a high prophylactic and therapeutic effect.