RESUMEN
Horse serum albumin has been shown to meet the requirements to protein preparations for microanalysis and thus to be suitable for use in kits of reagents for the radioimmunological determination of insulin and myoglobin, for the determination of tick-borne encephalitis virus antigen by the method of the enzyme immunoassay and for the stabilization of proteins in the hemagglutination test and the hemagglutination inhibition test.
Asunto(s)
Técnicas para Inmunoenzimas , Ovalbúmina , Radioinmunoensayo/métodos , Albúmina Sérica Bovina , Albúmina Sérica , Virología/métodos , Animales , Antígenos Virales/análisis , Virus de la Encefalitis Transmitidos por Garrapatas/inmunología , Estudios de Evaluación como Asunto , Pruebas de Inhibición de Hemaglutinación/métodos , Pruebas de Hemaglutinación/métodos , Caballos , Insulina/análisis , Mioglobina/análisisAsunto(s)
Encefalitis Transmitida por Garrapatas/diagnóstico , Animales , Eritrocitos , Fluoresceína-5-Isotiocianato , Fluoresceínas , Colorantes Fluorescentes , Pruebas de Hemaglutinación , Caballos , Sueros Inmunes , Inmunoglobulinas , Indicadores y Reactivos , Ratones , Conejos , Juego de Reactivos para Diagnóstico , TiocianatosRESUMEN
Virological and cytogenetic characterization of line RH and its two clones (RHk-20 and RHk-13/6), having different sensitivity to the tick-borne encephalitis virus (TBE), has been made. The RHk-13/6-cells are 10,000 times more sensitive to cytopathic effect of the tick-borne encephalitis virus in comparison with the RHk-20-cells. By its sensitivity to the virus the base line is in the intermediate position. Cytogenetic analysis revealed the variability interval decrease as to the chromosome number at cloning, and showed the structural stability of the line and clone karyotypes. The line RH and its clone K-20 have modal number of chromosomes equal to 65; the cell population with the chromosome number equal to 66 dominated in RHk-13/6. The application of methods of chromosome differential (G, C) staining and these for the detection of active nucleolar organizers (NO) permitted identifying the marker chromosomes and determining the difference between the clones and the line as associated with the presence of an additional homologue of chromosome 8 in the sensitive clone.
Asunto(s)
Aberraciones Cromosómicas , Virus de la Encefalitis Transmitidos por Garrapatas/patogenicidad , Riñón/ultraestructura , Línea Celular , Bandeo Cromosómico , Células Clonales/microbiología , Células Clonales/ultraestructura , Efecto Citopatogénico Viral , Humanos , Cariotipificación , Riñón/microbiología , MetafaseRESUMEN
A virulent strain of eastern equine encephalomyelitis virus produced in hamsters a lethal infection with severe lesions of nerve cells predominatly in the anterior parts of the brain. Parenchyma necroses occurred in the liver and lymphoid organs. Infectious virus and viral antigen were found in all the organs examined with the greatest accumulation in the brain. Attenuated variants of the virus produced in most hamsters an inapparent infection. In some animals without clinical signs, focal inflammatory-dystrophic lesions were found in the central nervous system (CNS) and the liver, as were immunomorphological changes in the lymphoid tissue. In the latter infectious virus could be detected for 6 days after inoculation (p.i.), whereas in the brain only viral antigen could be found up to 14 days. At 3 and 6 months p.i., no persistence of attenuated virus in the brain and lymphoid tissues could be established by organ culture and co-cultivation methods. Nor was virus antigen detected. No pathomorphological changes or proliferative-hypertrophic astrocyte reaction were found.