Effect of Unsymmetrical Dimethylhydrazine on Isolated Heart and Lymphatic Vessels.

Unsymmetrical dimethylhydrazine (UDMH, heptyl) and its derivatives are used in the rocket and space industry as components of rocket fuel. UDMH is a highly toxic compound exhibiting irritant, hepatotoxic, and neurotoxic properties. In this study, the toxic effect of heptyl on isolated rat heart and lymphatic vessels was demonstrated. Acute exposure to UDMH leads to vasoconstriction of the coronary vessels of the isolated heart and pronounced stimulation of isolated lymphangions starting from the concentration of 10-4 M. The changes are dose-dependent. After exposure to UDMH in high concentrations (10-2 M), the changes in the functional parameters became irreversible. The obtained results provide evidence for the organ-specific effect of UDMH on visceral muscle organs.

[INFLUENCE OF Ð -ENDORPHIN ON FUNCTIONAL ACTIVITY OF ISOLATED RAT HEART].

We have studied the effect of Р-endorphin (an endogenous opioid peptide) on the functional characteristics of isolated rat heart. In physiological concentrations (6.3 X 10-10 M) Р-endorphin induces a negative inotropic effect, accompanied by a slight decrease in the heart rate, and has no effect on the perfusion pressure of the coronary vessels. It is shown that the effect of Р-endorphin is mediated by 5-opioid receptors. ATP-dependent K+-channels contribute the greatest contribution to the implementation of the effect of Р-endorphin on the isolated heart of a rat. The role of voltage-dependent K+-channels and NO-mediated mechanisms in the implementation of the effects of Р-endorphin is minor.

[Effect of inert gas xenon on the functional state of nucleated cells of peripheral blood during freezing].

A new method of preservation of nucleated cells in the electric refrigerator with xenon. After slow freezing and storage is even one day at -80 °C persists for more than 60% leukocytes. Cell membranes are resistant to the vital dye. In 85% of granulocytes stored baseline lysosomal-cationic protein, reduced lipid peroxidation and antioxidant activity. Cryopreservation of biological objects in inert gases is a promising direction in the practice of medicine and can be an alternative to the traditional method using liquid nitrogen.

The use of inert gas xenon for cryopreservation of leukocytes.

We studied the possibility of cryopreservation of human blood nuclear cells under protection with inert gas xenon. A method for inducing clathrate anabiosis of leukocytes was developed that preserved the cells for practical use in biology and medicine.

Influence of pectins on NADPH oxidase and phagocytic activity of neutrophils during cryopreservation.

NADPH oxidase activation was studied by detecting the reactive oxygen species (ROS) and the dynamics of neutrophil phagocytic activity in blood before freezing and one day after exposure to -20 degrees C in the presence of pectic polysaccharides. Pectins (excluding lemnan) were found to increase the phagocytic activity of neutrophils, promote the formation of ROS in these cells and enhance NADPH oxidase activity in the following order: bergenan, comaruman, lemnan, apiuman, and potamogetonan. Cryoprotective action of lemnan and comaruman was proposed to be related to their unique chemical structures.

[Preservation of leukocytes at moderately low temperature].

It was shown that the use of little toxic cold-protective containing the glycerol protector endocellular action, HES--protector exocellular action, and "restored" component of the succinate 3-hydroxy-6-methyl-2- ethylpyridine preserves nuclear blood cells in a viable condition at -40 degrees C.

[Leucocytes conservation in the near-zero temperature].

The intensive metabolism and fast exhaustion of an energy potential of nuclear blood cells at positive temperatures essentially reduce terms of their storage in a viable condition. However for the clinical purposes given thensfusion environment is the extremely necessary. The popular method of preservation of cells at ultralow temperature (-196 degrees C) is limited in application since assumes use of the liquid nitrogen, the special equipment, trained engineering personnel. The work is devoted to development effective and simple in execution of a method of preservation of leukocytes in conditions of temperatures about zero (+2 degrees--0 degrees C) with use of a household elecrorefrigerator and new cryopreservation a solution.

A new method for preservation of viable leukocytes at near-zero temperatures.

We propose a new effective, available, and economic method for preservation of leukocytes at near-zero temperatures for 6 days using an original protecting solution.