RESUMEN
Interleukin-2 (IL-2) is recognized as a T cell growth factor. We have previously reported that human carcinoma cell lines are inhibited in growth by exogenous IL-2, which binds to the IL-2 receptor beta (IL-2Rbeta) chain ubiquitously expressed on the surface of tumor cells. A possibility was considered that IL-2Rbeta on carcinomas responsible for negative signaling was different from that expressed on hematopoietic cells. To investigate this possibility, mRNA for the IL-2Rbeta chain was amplified and compared in carcinoma and lymphoid cells. Using RT-PCR with pairs of sense-antisense oligonucleotide primers specific for the various regions of extracellular, transmembrane and intracellular domains of the IL-2Rbeta chain, we amplified mRNA obtained from three human carcinoma cell lines and human lymphoid cells as controls. The identity of the amplicons was confirmed by Southern analysis with the 32P-labeled cDNA probe coding for the entire span of the IL-2Rbeta chain. In addition, genomic DNA obtained from the tumor cell lines was sequenced to examine the possibility that a mutation is present in the gene coding for the intracellular IL-2Rbeta chain domain. No mutations or deletions were detected. The message for all three domains of the beta chain was identical in tumor cells and in normal lymphoid cells used as controls. Also, by Western blot and northern analyses no differences between IL-2Rbeta chain in tumors vs that expressed in lymphoid cells were demonstrable. The IL-2Rgamma chain, which participates in IL-2/IL-2R signaling pathway, was expressed in tumor cells. Expression of JAK1 transcripts in these cells was comparable to that in lymphocytes. However, RT-PCR analysis identified differences in expression of JAK3 splice variants (B and M) in tumor cells. These differences may be responsible for altered downstream signaling by IL-2. Overall, our data indicate that the same IL-2/IL-2R pathway is operative in human carcinomas and in normal epithelial or lymphoid cells.
Asunto(s)
Neoplasias/inmunología , Receptores de Interleucina-2/biosíntesis , Receptores de Interleucina-2/genética , Carcinoma de Células Renales , Cartilla de ADN , Neoplasias de Cabeza y Cuello , Humanos , Janus Quinasa 1 , Células Jurkat , Neoplasias Renales , Linfocitos/enzimología , Linfocitos/inmunología , Sustancias Macromoleculares , Neoplasias/genética , Oligonucleótidos Antisentido , Reacción en Cadena de la Polimerasa , Proteínas Tirosina Quinasas/biosíntesis , ARN Mensajero/biosíntesis , Receptores de Interleucina-2/química , Neoplasias Gástricas , Transcripción Genética , Células Tumorales CultivadasAsunto(s)
Actitud Frente a la Salud , Trastorno Bipolar/psicología , Trastorno Depresivo/psicología , Padres/psicología , Inventario de Personalidad/estadística & datos numéricos , Adolescente , Trastorno Bipolar/diagnóstico , Trastorno Bipolar/terapia , Niño , Trastorno Depresivo/diagnóstico , Trastorno Depresivo/terapia , Empatía , Terapia Familiar , Femenino , Humanos , Masculino , Padres/educación , Psicometría , Psicoterapia de GrupoRESUMEN
BACKGROUND: The cyclin-dependent kinase inhibitor gene p21Waf1/Cip1 plays a role in signaling cellular growth arrest. In response to DNA damage, p21 is induced by the p53 gene, thereby playing a direct role in mediating p53-induced G1 arrest. Alterations in this gene may adversely affect regulation of cellular proliferation and increase susceptibility for cancer. Two polymorphisms have previously been characterized in the p21 gene: a C-->A transversion at codon 31 (ser-->arg) and a C-->T transition 20 nucleotides downstream from the 3' end of exon 3. METHODS: The codon 31 polymorphism in exon 2 of the p21 gene was identified by restriction digestion (Alw26I) of products amplified by polymerase chain reaction (PCR). The polymorphism downstream from exon 3 of the p21 gene was identified by single strand conformation polymorphism (SSCP) analysis of PCR amplified products and was confirmed by PstI enzyme restriction digestion. DNA variant alleles were confirmed by direct DNA sequencing. The entire coding region and the promoter region (p53 binding domain) of the p21 gene were screened for mutations by SSCP analysis or DNA sequencing. RESULTS: The two polymorphisms were found in 18 of 96 tumor samples lacking p53 alterations (18.8%). Nine of 54 prostate adenocarcinoma samples (16.7%) contained both p21 variants, whereas 9 of 42 squamous cell carcinomas of the head and neck (21.4%) displayed both polymorphisms. Of the 110 controls examined, 10 (9.1%) had both alterations. Both p21 polymorphisms occurred together in all samples examined and there was no indication of mutation in the coding region of the p21 gene or in the p53 binding domain of the promoter region. CONCLUSIONS: These data suggest that p21 gene variants may play a role in increased susceptibility for the development of some types of cancer. In the current study, the authors demonstrated that the occurrence of these two polymorphisms is increased in prostate adenocarcinoma and squamous cell carcinoma of the head and neck. The polymorphic sites may be directly responsible for this apparent increased susceptibility or they may be linked to regulatory region alterations.
Asunto(s)
Ciclinas/genética , Neoplasias de Cabeza y Cuello/genética , Proteínas de Neoplasias/genética , Polimorfismo Genético/genética , Neoplasias de la Próstata/genética , Adenocarcinoma/genética , Carcinoma de Células Escamosas/genética , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , ADN de Neoplasias/genética , Humanos , Masculino , Polimorfismo Conformacional Retorcido-Simple , Análisis de Secuencia de ADNRESUMEN
Loss of heterozygosity (LOH) on chromosomes 3p and 9p has been documented in a variety of malignancies, which suggests the presence of tumor suppressor gene loci on these chromosomes. We have studied 77 oral carcinomas for LOH using 16 microsatellite markers distributed over 5 human chromosomes. Fifty-five (71%) of these tumors showed LOH at one or more loci. A significant proportion of LOH at the informative tumors was observed at chromosomes 3p and 9p: 58% and 48%, respectively. A majority of the tumors showed losses at multiple loci on chromosomes 3p or 9p or on both. Our results suggest that tumor suppressor genes located on the short arms of chromosomes 3 and 9 may be involved in the pathogenesis of oral carcinoma. These regions of deletion observed in oral cancers overlap those reported in other neoplasms. However, we did not find any evidence of these changes in tumor margins with early pathological changes.
Asunto(s)
Carcinoma de Células Escamosas/genética , Cromosomas Humanos Par 3 , Cromosomas Humanos Par 9 , Eliminación de Gen , Neoplasias de la Boca/genética , ADN de Neoplasias/análisis , ADN de Neoplasias/genética , ADN Satélite/análisis , ADN Satélite/genética , Heterocigoto , HumanosRESUMEN
Generalized genomic instability, detected as somatic changes in allele sizes at microsatellite loci in tumors compared to peripheral lymphocyte DNA, is a recently recognized mechanism of mutation in cancer. Such instability results from the somatic loss of DNA mismatch repair capability. Germ-line mutations at DNA mismatch repair loci confer susceptibility to colon cancer in hereditary non-polyposis colorectal cancer. Somatic loss of DNA mismatch repair has been reported in a large variety of other tumor types. Our goal was to determine the frequency of microsatellite instability in a large series of oral tumors. Out of 91 tumors analyzed for microsatellite instability, 6 (7%) showed microsatellite instability. Instability was observed at multiple loci with a range of 50-74% of loci affected. Alterations include both increase (74%) and decrease (26%) in allele sizes. The proportion of alleles affected ranged from 30-58% of all alleles. Our data suggest that somatic genomic instability plays a role in the pathogenesis of a small subset of oral tumors.
Asunto(s)
Carcinoma de Células Escamosas/genética , ADN de Neoplasias/genética , ADN Satélite/genética , Neoplasias de la Boca/genética , Adulto , Anciano , Alelos , Carcinoma de Células Escamosas/sangre , Cromosomas Humanos , Reparación del ADN , Replicación del ADN , Marcadores Genéticos , Humanos , Linfocitos/ultraestructura , Persona de Mediana Edad , Neoplasias de la Boca/sangreRESUMEN
Mutations of the p53 tumor suppressor gene are the most common genetic alterations associated with human cancer. Tumor-associated p53 mutations often show characteristic tissue-specific profiles which may infer environmentally induced mutational mechanisms. The p53 mutational frequency and spectrum were determined for 95 carcinomas of the upper and lower respiratory tract (32 lung and 63 upper respiratory tract). Mutations were identified at a frequency of 30% in upper respiratory tract (URT) tumors and 31% in lung tumors. All 29 identified mutations were single-base substitutions. Comparison of the frequency of specific base substitutions between lung and URT showed a striking difference. Transitions occurred at a frequency of 68% in URT, but only 30% in lung. Mutations involving G:C-->A:T transitions, which are commonly reported in gastric and esophageal tumors, were the most frequently identified alteration in URT (11/19). Mutations involving G:C-->T:A transversions, which were relatively common in lung tumors (3/10) and are representative of tobacco smoke-induced mutations were rare in URT tumors (1/19). Interestingly, G:C-->A:T mutations at CpG sites, which are characteristic of endogenous processes, were observed frequently in URT tumors (9/19) but only rarely in lung tumors (1/10), suggesting that both endogenous and exogenous factors are responsible for the observed differences in mutational spectra between the upper and lower respiratory systems.
Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/genética , Genes p53 , Neoplasias de Cabeza y Cuello/genética , Neoplasias Pulmonares/genética , Mutación Puntual , Neoplasias del Sistema Respiratorio/genética , Proteína p53 Supresora de Tumor/genética , Consumo de Bebidas Alcohólicas , Sustitución de Aminoácidos , Codón/genética , ADN de Neoplasias/genética , ADN de Neoplasias/aislamiento & purificación , Femenino , Humanos , Intrones , Masculino , Factores de Riesgo , Factores Sexuales , Fumar , Proteína p53 Supresora de Tumor/químicaRESUMEN
Thirty-two primary carcinomas of the lung and 17 carcinomas of the head and neck (HN) were systematically analyzed for p53 mutations in the highly conserved regions of the gene (exons 5-8). Frozen sections of the same tumors were stained immunohistochemically to assess the sensitivity and specificity of p53 expression as determined by the presence or absence of the protein. On the basis of histology, the lung tumors studied were divided into adenocarcinomas (AC; n = 15), squamous-cell carcinomas (SCC; n = 12), and large-cell carcinomas (LCC; n = 5). All the HN cancers were SCC. Mutations in the p53 gene were detected by direct sequencing of amplified polymerase chain reaction products in six AC of the lungs (40%), three SCC of the lungs (25%), and one LCC (20%), with an overall mutation frequency of 31%. Nine AC (60%) of the lungs, five SCC (42%), and four LCC (80%) were p53-positive by immunohistochemistry. Among HN cancers, p53 mutations were detected in seven tumors (41%). Nine HN tumors (53%) were positive for p53. Negative staining, despite the presence of p53 mutations, was confined to nonsense mutations with truncated p53 and to single-base mutations not causing any change in the amino acid. Although immunohistochemical staining for mutated p53 is sensitive and simple to perform as a screening method, it is not as specific for evaluation of p53 mutations in lung and HN cancers.
Asunto(s)
Adenocarcinoma/química , Carcinoma de Pulmón de Células no Pequeñas/química , Carcinoma de Células Escamosas/química , Neoplasias de Cabeza y Cuello/química , Neoplasias Pulmonares/química , Proteína p53 Supresora de Tumor/análisis , Secuencia de Bases , Humanos , Técnicas para Inmunoenzimas , Datos de Secuencia Molecular , Mutación Puntual/genética , Reacción en Cadena de la Polimerasa , Sensibilidad y Especificidad , Proteína p53 Supresora de Tumor/genéticaRESUMEN
A PstI polymorphism in the 3' flanking region of the p21Cip1/Waf1 cyclin-dependent kinase inhibitor gene is described. DNA sequencing analysis identified a C-->T base substitution in the 3' flanking region of the gene. This substitution leads to the destruction of a PstI site and results in a biallelic DNA polymorphism. This restriction fragment length polymorphism (RFLP) provides the first known genetic marker for this cell cycle regulatory gene.
Asunto(s)
Ciclinas/genética , Desoxirribonucleasas de Localización Especificada Tipo II/genética , Polimorfismo de Longitud del Fragmento de Restricción , Inhibidores de Proteínas Quinasas , Secuencia de Bases , Ciclo Celular/genética , Mapeo Cromosómico , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , ADN/análisis , Frecuencia de los Genes , Humanos , Datos de Secuencia MolecularRESUMEN
The K562 human chronic myelogenous leukemia (CML) cell line has attained widespread use as a model for studying hematologic malignancy and erythroid differentiation. Sequencing of the p53 gene in the K562 cell line demonstrated a mutation in exon 5 characterized by a single base insertion (cytosine) between codons 135 and 136. This frameshift mutation leads to an N-terminal truncated protein of 147 amino acids. Only the mutated sequence was present suggesting that the normal allele has been lost. Reverse transcription PCR (RT-PCR) detected a p53 transcript but Western blotting and immunohistochemical staining of cells failed to detect p53 protein. The identification of an inactivation mutation of p53 in the K562 cell line further supports the argument that p53 mutations play a role in myeloid blast transformation of CML.
Asunto(s)
Genes p53 , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Mutación , Proteína p53 Supresora de Tumor/biosíntesis , Secuencia de Aminoácidos , Secuencia de Bases , Western Blotting , Línea Celular , Codón , Citosina , Cartilla de ADN , Elementos Transponibles de ADN , ADN de Neoplasias/genética , ADN de Neoplasias/aislamiento & purificación , Exones , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Células Tumorales Cultivadas , Proteína p53 Supresora de Tumor/aislamiento & purificaciónAsunto(s)
Genes de Retinoblastoma , Gorilla gorilla/genética , Pan troglodytes/genética , Pongo pygmaeus/genética , Animales , Secuencia de Bases , Southern Blotting , ADN , Variación Genética , Humanos , Intrones , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Recombinación Genética , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
Human apolipoprotein A-IV (apoA-IV) exhibits a genetically determined structural polymorphism amenable to analysis by isoelectric focusing and immunoblotting techniques. We have determined the allele frequency and molecular basis of a unique ApoA-IV*5 allele which is widely distributed among blacks but is absent in other populations. The frequency of the ApoA-IV*5 allele in blacks (N = 308) was estimated to be 3.2%. In comparison to the common ApoA-IV*1 allele, analysis of coding and non-coding sequences of the ApoA-IV*5 allele revealed an in-frame insertion of 12 nucleotides near the carboxyl terminal region of the mature protein. The insertion involves an exact duplication of the second of the four repeats and codes for 4 amino acids glutamic acid (GAA), glutamine (CAG), glutamine (CAG), and glutamine (CAG) and is responsible for the charge shift of the the apoA-IV 5 isoform slightly toward the anode as compared to the wild type apoA-IV 1 isoform on the isoelectric focusing gel. This in-frame insertion occurs in a region which is highly conserved among rat, mouse, and humans. In addition to the 12 nucleotide insertion, the four individuals sequenced for the ApoA-IV*5 allele also revealed a same-sense mutation by replacing G to T at the third position of codon 316. Our preliminary data suggest that this unique black allele marker may be of potentially significance in studies of human lipid metabolism and in microevolution.
Asunto(s)
Apolipoproteínas A/genética , Apolipoproteínas A/fisiología , Población Negra/genética , Metabolismo de los Lípidos , Adolescente , Adulto , Alelos , Secuencia de Aminoácidos , Secuencia de Bases , ADN/análisis , Femenino , Humanos , Masculino , Datos de Secuencia Molecular , Mutación , Fenotipo , Reacción en Cadena de la Polimerasa , Polimorfismo Genético , South CarolinaRESUMEN
Germ line p53 point mutations have been reported for some families with Li-Fraumeni syndrome, a syndrome characterized by a dominantly inherited increased susceptibility for the development of early age of onset neoplasms of diverse origin in multiple family members. All of the initially reported p53 germ line mutations have been found exclusively within a single conserved, nonpolymorphic region of the gene between condons 245 and 258. The restricted distribution of these inherited mutations has led to speculation that germ line p53 mutations have unique properties [B. Vogelstein, Nature (Lond.), 348: 681-682, 1990]. We report here on the identification of a p53 germ line mutation at codon 133 (ATG----ACG) in nine members of an extended Li-Fraumeni syndrome family. This mutation leads to an amino acid substitution in the protein and is shown to completely cosegregate with Li-Fraumeni syndrome associated cancer in this family. Its location extends the region of the p53 gene where inherited mutations predisposing to cancer are observed and suggests that their distribution may be diverse.
Asunto(s)
Exones/genética , Genes p53/genética , Síndrome de Li-Fraumeni/genética , Mutación/genética , Adulto , Secuencia de Bases , Susceptibilidad a Enfermedades , Salud de la Familia , Femenino , Tamización de Portadores Genéticos , Humanos , Lactante , Masculino , Datos de Secuencia Molecular , Linaje , Reacción en Cadena de la PolimerasaRESUMEN
This study examines the association of Hirschsprung disease with Down syndrome and calculates the recurrence risk for families of Hirschsprung patients. Information was collected from 134 histologically diagnosed patients with Hirschsprung disease, from Children's Hospital in Pittsburgh, PA between 1950 and 1977. One hundred and three patients had short segment Hirschsprung disease which is defined as involvement up to and including the sigmoid colon. Thirty-one patients had the long segment type with aganglionosis extending in some cases to the small intestine. As in other studies, we found a significant association between Hirschsprung disease and Down syndrome in that 5.9% of probands had both. Mean maternal age of cases with both Hirschsprung disease and Down syndrome (33.5 years) was significantly different from controls (26.7 years) and non-Down syndrome Hirschsprung patients (26.6 years). The overall sex ratio for Hirschsprung disease was 3.6. Recurrence risks were dependent on proband sex and the degree of aganglionic involvement.