[The incidence of heat tolerant Campylobacter in rivers and lakes of the Warsaw region]. / Wystepowanie termotolerancyjnych bakterii rodzaju Campylobacter w rzekach i jeziorach okolic Warszawy.

The presence of thermotolerant Campylobacter in rivers and lakes of Warsaw region was examined with the detectability of 1 c.f.u./ml. Samples were taken from depth of water and from the surface of different objects deposited on the bottom. The results indicate that about 70% of water samples are contaminated with Campylobacter, whereas the contamination of the underwater objects is less prevalent. The species distribution was as follows: C. jejuni-65%, C. coli-22%, C. lari-13%. In vitro experiment was also performed to test the ability of Campylobacter to create biofilms on the surface of wood, metal and plastic, however no such property was revealed. From the analysis of presented results it was established that localization of the highest contamination is connected mainly with presence of municipal sewage and in less extent with the presence of the droppings of wild animals. The samples of water give the better reflection of the examined reservoir contamination than solid samples.

Antibiotic resistance of Campylobacter jejuni with reference to plasmid profiles of clinical and chicken isolates.

A total of 47 clinical isolates and 52 poultry isolates of Campylobacter jejuni were characterized by their resistance to 16 antimicrobial agents and by plasmid profiles on agarose gel electrophoresis. Almost all isolates were susceptible to erythromycin, chloramphenicol, gentamycin and nitrofuratoin. Plasmids were detected in 19% of C. jejuni strains isolated from feces of children patients and in 36% of strains isolated from chicken. The presence of plasmid DNA was not found to be correlated with any definite resistance, despite of the number of resistant strains was also higher among poultry isolates. Plasmids, as well, does not seem to be essential for colonization of alimentary tract of pathogenic activity.

[Tests of Helicobacter pylori survival in milk]. / Badania przezywalnosci Helicobacter pylori w mleku.

The survival of microaerophilic bacteria Helicobacter pylori (Hp) isolated from gastric mucosa was studied in 2 ml and 10 ml samples of milk contaminated with a suspension of these bacteria in 20% glucose solution. Milk was kept at 5-7 degrees C temperature in refrigerator and at room temperature. Hp was found in samples kept at 5-7 degrees C during 3 days. On the other hand, Hp was not found after 24-hours keeping at room temperature.