Paediatric surgical reality in Germany and visions for the future.

Over the past 2 centuries paediatric surgery has developed into a well-recognised, independent surgical speciality comparable to plastic surgery or thoracic and vascular surgery. About 550 members of the GAPS (German Association of Paediatric Surgery) annually treat about 2-3% of all 0-15-year-old children of the FRG and operate 32.5% of all children who need a surgical intervention in paediatric surgical centres. There are 83 paediatric surgical centres in the FRG. Most of them perform more than 2000 operations per year. Three newer developments are likely to influence the future of paediatric surgery in Germany: 1. demographic factors, particularly the massive reduction of the reproduction rate from factor 1 to 0.59; 2. the new German National Health Care System, called "Strukturreform 2000", which will introduce the Australian DRG system and a new administrative structure permitting only a restricted care of the patients under tight economic control; and 3. the European regulations of the UEMS/EBPS which the national Landesärztekammern will be required to adopt.

Localization of the prostacyclin receptor in human kidney.

BACKGROUND: Prostacyclin is an important mediator of renal hemodynamics. Furthermore, recent studies argue for a role of this arachidonic acid metabolite in the regulation of salt and water handling in the distal nephron. To gain insight into the network of prostacyclin signal transduction, we analyzed the intrarenal distribution of the prostacyclin receptor (IP receptor) in adult human kidney. METHODS: Specific polyclonal antibodies against a synthetic peptide of the human IP receptor were generated. By means of immunohistology the localization of IP receptor protein was studied. The mRNA expression for IP receptor was analyzed by in situ hybridization using specific cRNA probes. RESULTS: In human kidney sections both IP receptor-immunoreactive protein and mRNA were expressed in smooth muscle cells and endothelial cells. Expression of the IP receptor was observed in glomerular cells, namely mesangial cells, endothelial cells, and podocytes. Both mRNA and protein expression for IP receptor was observable in Tamm-Horsfall-negative distal tubules and collecting ducts. CONCLUSIONS: The vascular expression of the IP receptor is consistent with the known vasodilatory effect of prostacyclin in vascular beds. Glomerular expression argues for a role of this autacoid in the regulation of glomerular hemodynamics. The tubular distribution might point towards the involvement of prostacyclin in renal salt and water handling.