Survey on common reference intervals for general chemistry analytes in Hong Kong.

INTRODUCTION: Reference intervals (RIs) are essential tool for proper interpretation of results. There is a global trend towards implementing common RIs to avoid confusion and enhance patient management across different laboratories. However, local practices with respect to RIs lack harmonisation. METHODS: We have conducted the first local survey regarding RIs for 14 general chemistry analytes in 10 chemical pathology laboratories that employ four different analytical platforms (Abbott Architect, Beckman Coulter AU, Roche Cobas, and Siemens Dimension EXL). Analytical bias was assessed by an inter-laboratory results comparison of external quality assurance programmes. RESULTS: Sufficient inter-laboratory and inter-platform agreement regarding the 10 analytes (albumin, alanine aminotransferase, aspartate aminotransferase, chloride, gamma-glutamyl transferase, phosphate, potassium, sodium, total protein, and urea) were demonstrated. However, the RIs were heterogeneous across all laboratories, with percentage differences of the upper RI value of up to 47% for aspartate aminotransferase (absolute difference of 16 U/L), 29% for urea (1.8 mmol/L), and 18% for potassium (0.8 mmol/L). The percentage difference between lower RI values was up to 24% for urea (0.6 mmol/L), 22% for phosphate (0.16 mmol/L), and 8% for total protein (5 g/L). The coefficients of variation of the upper RI values of potassium and sodium were 1.2 times and 1.0 times of their corresponding between-subject biological variation, respectively, representing unnecessary variations that are overlooked and unchecked in current practice. CONCLUSIONS: We recommend the use of common RIs for general chemistry analytes in Hong Kong to prevent interpreter confusion, improve electronic data transfer, and unite laboratory practice. This is the first local study on this topic, and our data can lay the groundwork for increasing harmonisation of RIs across more laboratory tests.

Dysmenorrhoea among Hong Kong university students: prevalence, impact, and management.

OBJECTIVE. To evaluate the prevalence of dysmenorrhoea, its impact, and management approaches in Hong Kong university students, and to compare between medical and non-medical students for any potential differences in coping strategies. DESIGN. Cross-sectional questionnaire survey. SETTING. The University of Hong Kong, Hong Kong. PARTICIPANTS. A total of 240 undergraduate (128 medical and 112 non-medical) students. MAIN OUTCOME MEASURES. Data on the presence and severity of dysmenorrhoea, its impact on daily life, management approaches, specific strategies, and their self-perceived effectiveness were obtained and analysed. RESULTS. In these subjects, the prevalence of dysmenorrhoea was 80% (95% confidence interval, 75-85%) with a mean (standard deviation) pain score of 5.0 (1.7). The most common impacts on daily life included reduced ability to concentrate and/or disturbance with study (75%) and changes in normal physical activity (60%). Only 6% sought medical advice, while 70% practised self-management. Pain scores and pain affecting normal physical activities were important predictive factors for self-management and for management based on pharmacological or non-pharmacological means. The commonest specific strategies used were a warm beverage (62%), paracetamol (57%), and sleeping (45%), while the most effective strategies were non-steroidal anti-inflammatory drugs (100%), traditional Chinese medicine (93%), and dietary/nutritional supplements (92%). Regarding the comparison of medical and non-medical students, the former used fewer pharmacological strategies among the various management approaches investigated. CONCLUSION. With data showing dysmenorrhoea as a very common condition having a significant impact in the Hong Kong community, primary care doctors should reassure young women with dysmenorrhoea that it is a common experience in the same age-group. Health education on the existence of effective treatment from medical practitioners could help women whose dysmenorrhoea was not controlled by self-management.

Sentence processing deficits in two Cantonese aphasic patients.

This paper reports the performances of two Cantonese aphasics on tasks examining their sentence processing deficits. The data on sentence comprehension show that thematically noncanonical sentences, full passives, and subject-gap sentences present greater difficulty to these patients than canonical sentences, truncated passives, and object-gap sentences, respectively. These patterns are consistent with previous observations on Chinese aphasics and are expected given the structural differences between Chinese and English. In a Cantonese grammaticality judgment test, a set of structures are identified that can elicit clear judgments from normal subjects and aphasics, contrary to the claim that grammaticality judgments in Chinese are probabilistic and fragile. Most interestingly, the patients' overall performance patterns reveal a double dissociation between sentence comprehension and judgment of sentence well-formedness, suggesting that the two tasks are supported by independent processing mechanisms.

The effect of trifluoperazine and R 24 571 on the K+-evoked release of 5-hydroxytryptamine from superfused synaptosomes.

The effect of trifluoperazine (10 microM) and of R 24571 (0.1 and 1.0 microM) on the release of [3H]-5-HT from superfused synaptosomes from rat brain was studied. Trifluoperazine, but not R 24571, produced a rapid transient increase in the basal efflux of [3H]-5-HT. This effect may be due to the cleavage of either Ca2+ or [3H]-5-HT from binding sites on the synaptosomal membrane. In contrast to the effects on the basal efflux of [3H]-5-HT, both drugs significantly inhibited the release of [3H]-5-HT in response to K+ depolarisation. This effect of trifluoperazine did not appear to be due to the known membrane stabilising effects of the drug, but appeared more likely to be due to the calmodulin-inhibiting effect of the drug. The results of this study support the view that calmodulin may be an important intermediate linking depolarisation with transmitter release in nerve terminals.