RESUMEN
Kernel thickness is one of the most important traits in kernel structure, and is related to yield. To ascertain its genetic information more clearly, an immortal recombinant inbred line segregation population was used to map the quantitative trait loci (QTLs) for kernel thickness. As a result, two QTLs were identified on chromosome 9; both of them had negative additive effects, and could decrease kernel thickness to some extent. The QTLs explained 25.8% of the total phenotypic variation. These results advance our understanding of the genetic basis of kernel thickness in maize-breeding programs.
Asunto(s)
Sitios de Carácter Cuantitativo , Carácter Cuantitativo Heredable , Zea mays/genética , Mapeo Cromosómico , Cromosomas de las Plantas , Pruebas Genéticas , Endogamia , Escala de Lod , FenotipoRESUMEN
Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcription-polymerase chain reaction (RT-PCR). The result of sequence analysis for the amplified fragment revealed that the cloned actin gene was 1012 bp in length (GenBank accession No. KC751541.1) and contained a 924-bp coding region and encoded a protein consisting of 307 amino acids. Homologous alignment showed that it shared over 80 and 96% identity with the nucleotide and amino acid sequences of actin from other plants, respectively. In addition, the cloned gene was used for phylogenetic analyses based on the deduced amino acid sequences, and the results suggested that the actin gene is highly conserved in evolution. The findings of this study will be useful for investigating the expression patterns of other genes in Aloe.
Asunto(s)
Actinas/genética , Aloe/genética , Filogenia , Hojas de la Planta/genética , Proteínas de Plantas/genética , Actinas/química , Aloe/clasificación , Secuencia de Aminoácidos , Secuencia de Bases , Evolución Biológica , Clonación Molecular , Secuencia Conservada , Expresión Génica , Datos de Secuencia Molecular , Hojas de la Planta/clasificación , Proteínas de Plantas/química , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Alineación de SecuenciaRESUMEN
Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites, which make it difficult to isolate high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of aloe, respectively. The obtained samples presented good quality and integrity; thus, they could be further used for many downstream molecular experiments. These reported protocols for DNA and RNA extraction offered a valuable reference for other related studies.
Asunto(s)
Aloe/química , Fraccionamiento Químico/métodos , ADN de Plantas/aislamiento & purificación , ARN de Planta/aislamiento & purificaciónRESUMEN
Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.